Mathematical analysis and modeling of DNA segregation mechanisms

  • Received: 02 January 2017 Accepted: 04 May 2017 Published: 01 April 2018
  • MSC : Primary: 46N60, 34A09, 35K57, 32W50; Secondary: 46N20

  • The precise regulation of cell life division is indispensable to the reliable inheritance of genetic material, i.e. DNA, in successive generations of cells. This is governed by dedicated biochemical networks which ensure that all requirements are met before transition from one phase to the next. The Spindle Assembly Checkpoint (SAC) is an evolutionarily mechanism that delays mitotic progression until all chromosomes are properly linked to the mitotic spindle. During some asymmetric cell divisions, such as those observed in budding yeast, an additional mechanism, the Spindle Position Checkpoint (SPOC), is required to delay exit from mitosis until the mitotic spindle is correctly aligned. These checkpoints are complex and their elaborate spatiotemporal dynamics are challenging to understand intuitively. In this study, bistable mathematical models for both activation and silencing of mitotic checkpoints were constructed and analyzed. A one-parameter bifurcation was computed to show the realistic biochemical switches considering all signals. Numerical simulations involving systems of ODEs and PDEs were performed over various parameters, to investigate the effect of the diffusion coefficient. The results provide systems-level insights into mitotic transition and demonstrate that mathematical analysis constitutes a powerful tool for investigation of the dynamic properties of complex biomedical systems.

    Citation: Bashar Ibrahim. Mathematical analysis and modeling of DNA segregation mechanisms[J]. Mathematical Biosciences and Engineering, 2018, 15(2): 429-440. doi: 10.3934/mbe.2018019

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  • The precise regulation of cell life division is indispensable to the reliable inheritance of genetic material, i.e. DNA, in successive generations of cells. This is governed by dedicated biochemical networks which ensure that all requirements are met before transition from one phase to the next. The Spindle Assembly Checkpoint (SAC) is an evolutionarily mechanism that delays mitotic progression until all chromosomes are properly linked to the mitotic spindle. During some asymmetric cell divisions, such as those observed in budding yeast, an additional mechanism, the Spindle Position Checkpoint (SPOC), is required to delay exit from mitosis until the mitotic spindle is correctly aligned. These checkpoints are complex and their elaborate spatiotemporal dynamics are challenging to understand intuitively. In this study, bistable mathematical models for both activation and silencing of mitotic checkpoints were constructed and analyzed. A one-parameter bifurcation was computed to show the realistic biochemical switches considering all signals. Numerical simulations involving systems of ODEs and PDEs were performed over various parameters, to investigate the effect of the diffusion coefficient. The results provide systems-level insights into mitotic transition and demonstrate that mathematical analysis constitutes a powerful tool for investigation of the dynamic properties of complex biomedical systems.


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    [1] [ S. F. Bakhoum,G. Genovese,D. A. Compton, Deviant kinetochore microtubule dynamics underlie chromosomal instability, Curr Biol, 19 (2009): 1937-1942.
    [2] [ A. K. Caydasi, B. Ibrahim and G. Pereira, Monitoring spindle orientation: Spindle position checkpoint in charge Cell Div 5 (2010), p28.
    [3] [ A. K. Caydasi,B. Kurtulmus,M. I. L. Orrico,A. Hofmann,B. Ibrahim,G. Pereira, Elm1 kinase activates the spindle position checkpoint kinase Kin4, J Cell Biol, 190 (2010): 975-989.
    [4] [ A. K. Caydasi, M. Lohel, G. Grünert, P. Dittrich, G. Pereira and B. Ibrahim, A dynamical model of the spindle position checkpoint Mol Syst Biol 8 (2012), p582.
    [5] [ L. M. Cherry,A. J. Faulkner,L. A. Grossberg,R. Balczon, Kinetochore size variation in mammalian chromosomes: An image analysis study with evolutionary implications, J Cell Sci, 92 (1989): 281-289.
    [6] [ E. J. Doedel, AUTO: A program for the automatic bifurcation analysis of autonomous systems, Congr Numer, 30 (1981): 265-284.
    [7] [ A. Doncic,E. Ben-Jacob,N. Barkai, Evaluating putative mechanisms of the mitotic spindle checkpoint, Proc Natl Acad Sci U S A, 102 (2005): 6332-6337.
    [8] [ B. Ermentrout, Simulating, Analyzing, and Animating Dynamical Systems: A Guide to Xppaut for Researchers and Students (society for industrial and applied mathematics, philadelphia), 2002.
    [9] [ D. Görlich, G. Escuela, G. Gruenert, P. Dittrich and B. Ibrahim, Molecular codes through complex formation in a model of the human inner kinetochore Biosemiotics 7 (2014), p223.
    [10] [ G. Gruenert, B. Ibrahim, T. Lenser, M. Lohel, T. Hinze and P. Dittrich, Rule-based spatial modeling with diffusing, geometrically constrained molecules BMC Bioinf 11 (2010), p307.
    [11] [ G. Gruenert,J. Szymanski,J. Holley,G. Escuela,A. Diem,B. Ibrahim,A. Adamatzky,J. Gorecki,P. Dittrich, Multi-scale modelling of computers made from excitable chemical droplets, IJUC, 9 (2013): 237-266.
    [12] [ R. Henze,J. Huwald,N. Mostajo,P. Dittrich,B. Ibrahim, Structural analysis of in silico mutant experiments of human inner-kinetochore structure, Bio Systems, 127 (2015): 47-59.
    [13] [ B. Ibrahim, In silico spatial simulations reveal that MCC formation and excess BubR1 are required for tight inhibition of the anaphase-promoting complex, Mol Biosyst, 11 (2015): 2867-2877.
    [14] [ B. Ibrahim, Spindle assembly checkpoint is sufficient for complete Cdc20 sequestering in mitotic control, Comput Struct Biotechnol J, 13 (2015): 320-328.
    [15] [ B. Ibrahim, Systems biology modeling of five pathways for regulation and potent inhibition of the anaphase-promoting complex (APC/C): Pivotal roles for MCC and BubR1, Omics, 19 (2015): 294-305.
    [16] [ B. Ibrahim, Toward a systems-level view of mitotic checkpoints, Prog Biophys Mol Biol, 117 (2015): 217-224.
    [17] [ B. Ibrahim, A mathematical framework for kinetochore-driven activation feedback in the mitotic checkpoint, Bull Math Biol, 79 (2017): 1183-1200.
    [18] [ B. Ibrahim, S. Diekmann, E. Schmitt and P. Dittrich, In-silico modeling of the mitotic spindle assembly checkpoint PLoS One 3 (2008), e1555.
    [19] [ B. Ibrahim,P. Dittrich,S. Diekmann,E. Schmitt, Stochastic effects in a compartmental model for mitotic checkpoint regulation, J Integr Bioinform, 4 (2007): 77-88.
    [20] [ B. Ibrahim,P. Dittrich,S. Diekmann,E. Schmitt, Mad2 binding is not sufficient for complete Cdc20 sequestering in mitotic transition control (an in silico study), Biophys Chem, 134 (2008): 93-100.
    [21] [ B. Ibrahim,R. Henze, Active transport can greatly enhance Cdc20:Mad2 formation, Int J Mol Sci, 15 (2014): 19074-19091.
    [22] [ B. Ibrahim,R. Henze,G. Gruenert,M. Egbert,J. Huwald,P. Dittrich, Spatial rule-based modeling: A method and its application to the human mitotic kinetochore, Cells, 2 (2013): 506-544.
    [23] [ B. Ibrahim,E. Schmitt,P. Dittrich,S. Diekmann, In silico study of kinetochore control, amplification, and inhibition effects in MCC assembly, Bio Systems, 95 (2009): 35-50.
    [24] [ G. J. Kops,B. A. Weaver,D. W. Cleveland, On the road to cancer: Aneuploidy and the mitotic checkpoint, Nat Rev Cancer, 5 (2005): 773-785.
    [25] [ P. Kreyssig, G. Escuela, B. Reynaert, T. Veloz, B. Ibrahim and P. Dittrich, Cycles and the qualitative evolution of chemical systems PLoS One 7 (2012), e45772.
    [26] [ P. Kreyssig,C. Wozar,S. Peter,T. Veloz,B. Ibrahim,P. Dittrich, Effects of small particle numbers on long-term behaviour in discrete biochemical systems, Bioinformatics, 30 (2014): i475-i481.
    [27] [ M. Lohel,B. Ibrahim,S. Diekmann,P. Dittrich, The role of localization in the operation of the mitotic spindle assembly checkpoint, Cell Cycle, 8 (2009): 2650-2660.
    [28] [ S. Marques,J. Fonseca,P. MA Silva,H. Bousbaa, Targeting the spindle assembly checkpoint for breast cancer treatment, Curr Cancer Drug Targets, 15 (2015): 272-281.
    [29] [ H. B. Mistry,D. E. MacCallum,R. C. Jackson,M. A. J. Chaplain,F. A. Davidson, Modeling the temporal evolution of the spindle assembly checkpoint and role of Aurora B kinase, Proc Natl Acad Sci U S A, 105 (2008): 20215-20220.
    [30] [ A. Musacchio,E. D. Salmon, The spindle-assembly checkpoint in space and time, Nat Rev Mol Cell Biol, 8 (2007): 379-393.
    [31] [ C. L. Rieder,R. W. Cole,A. Khodjakov,G. Sluder, The checkpoint delaying anaphase in response to chromosome monoorientation is mediated by an inhibitory signal produced by unattached kinetochores, J Cell Biol, 130 (1995): 941-948.
    [32] [ C. L. Rieder,A. Schultz,R. Cole,G. Sluder, Anaphase onset in vertebrate somatic cells is controlled by a checkpoint that monitors sister kinetochore attachment to the spindle, J Cell Biol, 127 (1994): 1301-1310.
    [33] [ A. D. Rudner,A. W. Murray, The spindle assembly checkpoint, Curr Opin Cell Biol, 8 (1996): 773-780.
    [34] [ R. P. Sear,M. Howard, Modeling dual pathways for the metazoan spindle assembly checkpoint, Proc Natl Acad Sci U S A, 103 (2006): 16758-16763.
    [35] [ L. F. Shampine,M. W. Reichelt, The matlab ode suite, SIAM J Sci Comput, 18 (1997): 1-22.
    [36] [ R. D. Skeel,M. Berzins, A method for the spatial discretization of parabolic equations in one space variable, SIAM J Sci Comput, 11 (1990): 1-32.
    [37] [ F. Stegmeier,M. Rape,V. M. Draviam,G. Nalepa,M. E. Sowa,X. L. Ang,E. R. McDonald,M. Z. Li,G. J. Hannon,P. K. Sorger,M. W. Kirschner,J. W. Harper,S. J. Elledge, Anaphase initiation is regulated by antagonistic ubiquitination and deubiquitination activities, Nature, 446 (2007): 876-881.
    [38] [ S. Tschernyschkow,S. Herda,G. Gruenert,V. Döring,D. Görlich,A. Hofmeister,C. Hoischen,P. Dittrich,S. Diekmann,B. Ibrahim, Rule-based modeling and simulations of the inner kinetochore structure, Prog Biophys Mol Biol, 113 (2013): 33-45.
    [39] [ A. Verdugo, P. K. Vinod, J. J. Tyson and B. Novak, Molecular mechanisms creating bistable switches at cell cycle transitions Open Biol 3 (2013), 120179.
    [40] [ Z. Wang,J. V. Shah,M. W. Berns,D. W. Cleveland, In vivo quantitative studies of dynamic intracellular processes using fluorescence correlation spectroscopy, Biophys J, 91 (2006): 343-351.
    [41] [ T. Wilhelm, The smallest chemical reaction system with bistability BMC Syst Biol 3 (2009), p90.
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