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Research article Special Issues

Cell growth on electrospun nanofiber mats from polyacrylonitrile (PAN) blends

  • Received: 14 January 2020 Accepted: 12 March 2020 Published: 16 March 2020
  • Nanofiber mats can be produced by electrospinning from diverse polymers and polymer blends as well as with embedded ceramics, metals, etc. The large surface-to-volume ratio makes such nanofiber mats a well-suited substrate for tissue engineering and other cell growth experiments. Cell growth, however, is not only influenced by the substrate morphology, but also by the sterilization process applied before the experiment as well as by the chemical composition of the fibers. A former study showed that cell growth and adhesion are supported by polyacrylonitrile/gelatin nanofiber mats, while both factors are strongly reduced on pure polyacrylonitrile (PAN) nanofibers. Here we report on the influence of different PAN blends on cell growth and adhesion. Our study shows that adding ZnO to the PAN spinning solution impedes cell growth, while addition of maltodextrin/pea protein or casein/gelatin supports cell growth and adhesion.

    Citation: Daria Wehlage, Hannah Blattner, Al Mamun, Ines Kutzli, Elise Diestelhorst, Anke Rattenholl, Frank Gudermann, Dirk Lütkemeyer, Andrea Ehrmann. Cell growth on electrospun nanofiber mats from polyacrylonitrile (PAN) blends[J]. AIMS Bioengineering, 2020, 7(1): 43-54. doi: 10.3934/bioeng.2020004

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  • Nanofiber mats can be produced by electrospinning from diverse polymers and polymer blends as well as with embedded ceramics, metals, etc. The large surface-to-volume ratio makes such nanofiber mats a well-suited substrate for tissue engineering and other cell growth experiments. Cell growth, however, is not only influenced by the substrate morphology, but also by the sterilization process applied before the experiment as well as by the chemical composition of the fibers. A former study showed that cell growth and adhesion are supported by polyacrylonitrile/gelatin nanofiber mats, while both factors are strongly reduced on pure polyacrylonitrile (PAN) nanofibers. Here we report on the influence of different PAN blends on cell growth and adhesion. Our study shows that adding ZnO to the PAN spinning solution impedes cell growth, while addition of maltodextrin/pea protein or casein/gelatin supports cell growth and adhesion.



    Goncharov was the first to find relations between the Grassmannian complex of projective configurations and Bloch-Suslin complex for weight n=2, and to the Goncharov's motivic complex for weight n=3 (see [3]). This idea leads to the remarkable proof of Zagier's conjecture for weights n=2,3 (see [4]). On the other hand, Cathelineau introduced the tangent form of the Bloch-Suslin complex and provided some suggestions about the tangent form of Goncharov's complex (see [1]).

    The main idea of this article is to view geometric features of tangent groups, TB2(F) and TB3(F), where TB2(F) is the tangent form of Bloch group B2(F) (see [1]), and TB3(F) is the tangent form of Goncharov's group B3(F) (see §3.2) for any field F. To accomplish this task, we define morphisms τ20,ε, τ21,ε, (between the Grassamannian complex of geometric configurations and tangent to the Bloch-Suslin complex) and τ30,ε, τ31,ε, τ32,ε (between the Grassamannian complex of geometric configurations and tangent to the Goncharov's complex) for weights n=2,3. Due to these morphisms, we get diagrams which are shown to be commutative (main result Theorem 3.7). The major techniques for showing our main result, are to invoke combinatorics in the symmetric group S6 and to rewrite triple ratios in the product of two projected cross-ratios. Here, we use permutations of symmetric group S6 in the alternation sums. The alternation sum Alt6 in our map τ32,ε has 6! terms, but due to inversion and cyclic symmetry, it reduces to 6!/(3!)=120 terms.

    The cross ratio identity over a field F was first defined by Siegel (see [6]). To view the geometry of configurations in tangent groups, it is required to introduce an analogue to the Siegel cross-ratio identity for the determinants of matrices of order 2×2 (see Lemma 2.1) that can also be extended to 3×3 determinants of matrices. These analogues and Lemma 2.1 enabled us to produce the analogues of cross-ratios and triple ratios.

    On the basis of these analogues, we find morphisms between the Grassmannian subcomplex C(AnF[ε]2,d) and tangent to the Bloch-Suslin and to Goncharov complexes (see §3.1 and §3.2). The proof of the main result requires projected five term relation in TB2(F). To serve this purpose, we prove the existence of the projected five term relation in TB2(F) (see Lemma 3.4). This relation is also an analogue of Goncharov's projected five term relation in B2(F).

    In §3.2, we define the tangent group TB3(F) which was first hypothetically defined in §9 of [1]. On the basis of our definition, we mimic construction of TB3(F) with the F-vector space βD3(F) ([5]) and reproduce Cathelineau's 22-term functional equation for TB3(F).

    Let F be a field of characteristic 0. For ν1, we denote the νth truncated polynomial ring over F by F[ε]ν:=F[ε]/εν. Further define Cm(AnF[ε]ν) as a free abelian group generated by m generic points in AnF[ε]ν (an n dimensional affine space over F[ε]ν). Here, we are not considering degenerate points and are also assuming that no two points coincide and no three points lie on a line. Now for n=2 and ν=2, any ηi=(aibi)A2F{(00)} and ηi,ε:=(ai,εbi,ε)A2F, we put ηi=(ai+ai,εεbi+bi,εε)=(aibi)+(ai,εbi,ε)ε=ηi+ηi,εε and define a boundary map

    d:Cm+1(A2F[ε]2)Cm(A2F[ε]2)
    d:(η0,,ηm)mi=0(1)i(η0,,ˆηi,,ηm).

    Let ωV2 be a volume element formed in V2:=A2F and Δ(ηi,ηj)=ω,ηiηj, where ηi,ηjA2F. Here we define

    Δ(ηi,ηj)=Δ(ηi,ηj)ε0+Δ(ηi,ηj)ε1ε

    where

    Δ(ηi,ηj)ε0=Δ(ηi,ηj) and Δ(ηi,ηj)ε1=Δ(ηi,ηj,ε)+Δ(ηi,ε,ηj).

    More generally for ν=n+1, we have

    ηi=ηi+ηi,εε+ηi,ε2ε2++ηi,εnεn and ηi,ε0=ηi

    and we get

    Δ(ηi,ηj)=Δ(ηi,ηj)+Δ(ηi,ηj)εε+Δ(ηi,ηj)ε2ε2++Δ(ηi,ηj)εnεn,

    where

    Δ(ηi,ηj)εn=Δ(ηi,ηj,εn)+Δ(ηi,ε,ηj,εn1)++Δ(ηi,εn,ηj)

    Consider the Siegel cross-ratio identity for the 2×2 determinants of four vectors in C4(A2F) (see [3], [6])

    Δ(η0,η1)Δ(η2,η3)=Δ(η0,η2)Δ(η1,η3)Δ(η0,η3)Δ(η1,η2) (2.1)

    With the above notation, an analogue to the Siegel cross-ratio identity turns out to be true for A2F[ε]n+1, and we can extract further results which are essential for the proof of our main results. Throughout this section we will assume that Δ(ηi,ηj)0 for ij.

    Lemma 2.1. For (η0,η1,η2,η3)C4(A2F[ε]n+1), we have

    Δ(η0,η1)Δ(η2,η3)=Δ(η0,η2)Δ(η1,η3)Δ(η0,η3)Δ(η1,η2) (2.2)

    where

    ηi=ηi+ηi,εε+ηi,ε2ε2++ηi,εnεnandηi,ε0=ηi
    Δ(ηi,ηj)=Δ(ηi,ηj)+Δ(ηi,ηj)εε+Δ(ηi,ηj)ε2ε2++Δ(ηi,ηj)εnεn

    for

    Δ(ηi,ηj)εn=Δ(ηi,ηj,εn)+Δ(ηi,ε,ηj,εn1)++Δ(ηi,εn,ηj)

    Proof. For r=0,,n, we can write η=(r0ηrεrr0ηrεr) and m=(r0mrεrr0mrεr).

    Now we have

    Δ(η,m)=|r0ηrεrr0mrεrr0ηrεrr0mrεr|=r0(rk=0ηkmrkrk=0ηkmrk)εr=r0(rk=0Δ(ηk,mrk))εr

    Hence

    Δ(η0,η1)Δ(η2,η3)=r0(rk=0Δ(η0,k,η1,rk))εrs0(rj=0Δ(η0,j,η1,rj))εs=t0εt(tr=0(rk=0Δ(η0,k,η1,rk)trj=0Δ(η2,j,η3,trj)))=t0εt(tr=0(rk=0trj=0Δ(η0,k,η1,rk)Δ(η2,j,η3,trj))),

    and similarly for Δ(η0,η2)Δ(η1,η3) and Δ(η0,η3)Δ(η1,η2). Hence we use the validity of (2.1) to deduce the analogue for Δ(ηi,ηj)'s in place of Δ(ηi,ηj) passing from the ring F[[ε]] of power series to a truncated polynomial ring, say to F[ε]n+1.

    For the special cases; we find the identity (2.1) for n=0, while for n=1 we have the following identity which will be used extensively below:

    Δ(η0,η1)Δ(η2,η3)ε+Δ(η2,η3)Δ(η0,η1)ε={Δ(η0,η2)Δ(η1,η3)ε+Δ(η1,η3)Δ(η0,η2)ε}{Δ(η0,η3)Δ(η1,η2)ε+Δ(η1,η2)Δ(η0,η3)ε}. (2.3)

    if we write

    (ab)εn:=aεnbε0+aεn1bε++aε0bεn

    then (2.3) can be more concisely written as

    {Δ(η0,η1)Δ(η2,η3)}ε={Δ(η0,η2)Δ(η1,η3)}ε{Δ(η0,η3)Δ(η1,η2)}ε.

    Now we have enough tools to find the cross-ratios of four points over the truncated polynomial ring F[ε]ν. The identity (2.2) of Lemma 2.1 enables us to compute this ratio in F[ε]ν for ν=n+1. First we define the cross-ratio of four points (η0,,η3)C4(A2F[ε]n+1) as

    r(η0,,η3)=Δ(η0,η3)Δ(η1,η2)Δ(η0,η2)Δ(η1,η3)

    We also expand r(η0,,η3) as a truncated polynomial over F[ε]n+1

    r(η0,,η3)=(rε0+rεε+rε2ε2++rεnεn)(η0,,η3) (2.4)

    After truncating this for n=0, one gets

    r(η0,,η3)=rε0(η0,,η3)=r(η0,,η3)=Δ(η0,η3)Δ(η1,η2)Δ(η0,η2)Δ(η1,η3) (2.5)

    If we truncate (2.4) for n=1 then the coefficient of ε0 will remain the same as for n=0, thus we only need to compute the coefficient of ε in the following way:

    After considering (η0,,η3)C4(A2F[ε]2) in a generic position, we get

    r(η0,,η3)=Δ(η0,η3)Δ(η1,η2)Δ(η0,η2)Δ(η1,η3)={Δ(η0,η3)+Δ(η0,η3)εε}{Δ(η1,η2)+Δ(η1,η2)εε}{Δ(η0,η2)+Δ(η0,η2)εε}{Δ(η1,η3)+Δ(η1,η3)εε}

    If a0F then 1a+bε=1aba2εF[ε]2 (this is the same as the inversion relation in TB2(F) discussed later in §3.3).

    Let us simplify the above obtained result by multiplying the inverses of denominators and separate the coefficients of ε0 and ε. The coefficient of ε becomes

    rε(η0,,η3)={Δ(η0,η3)Δ(η1,η2)}εΔ(η0,η2)Δ(η1,η3)r(η0,,η3){Δ(η0,η2)Δ(η1,η3)}εΔ(η0,η2)Δ(η1,η3) (2.6)

    Let us trancate it for n=2, i.e., (η0,,η3)C4(A2F[ε]3). To make computations easy, we write (ηi,ηj) instead of Δ(ηi,ηj)

    r(η0,,η3)={(η0,η3)+(η0,η3)εε+(η0,η3)ε2ε2}{(η1,η2)+(η1,η2)εε+(η1,η2)ε2ε2}{(η0,η2)+(η0,η2)εε+(η0,η3)ε2ε2}{(η1,η3)+(η1,η3)εε+(η1,η3)ε2ε2}

    simplify and then separate the coefficients of ε0, ε1 and ε2. The coefficients of ε0 and ε1 are the same as we computed in (2.5) and (2.6) respectively, and the coefficient of ε2 is

    rε2(η0,,η3)={(η0,η3)(l1,l2)}ε2(η0,η2)(η1,η3)rε(η0,,η3){(η0,η2)(η1,η3)}ε(η0,η2)(η1,η3)r(η0,,η3){(η0,l2)(η1,η3)}ε2(η0,η2)(η1,η3) (2.7)

    Remark 2.2. The computation of coefficient of εn, which is rεn(η0,,η3), in the truncated polynomial (2.4) will give us the following:

    nk=0({Δ(η0,η2)Δ(η1,η3)}εkrεnk(η0,,η3))={Δ(η0,η3)Δ(η1,η2)}εn,

    where Δ(ηi,ηj)0 for ij and (η0,,η3)C4(A2F[ε]n+1).

    First, we define a triple-ratio r3:C6(A3F)F as (see [4])

    r3(η0,,η5)=Alt6Δ(η0,η1,η3)Δ(η1,η2,η4)Δ(η2,η0,η5)Δ(η0,η1,η4)Δ(η1,η2,η5)Δ(η2,η0,η3)

    where C6(A3F) is a free abelian group generated by the configurations of six points in A3F and A3F is a three dimensional affine space over a field F. Here, we will discuss triple-ratio (generalized cross-ratio) of 6 points, i.e., (η0,,η5)C6(A3F[ε]ν) for ν=n+1. The calculations in triple-ratio are similar to the cross-ratio of 4 points (η0,,η3)C4(A2F[ε]ν). Let's consider ν=2 since the other cases are not required.

    We take (η0,,η5)C6(A3F[ε]2), for any ηi(η0,,η5)

    li=(ai+ai,εεbi+bi,εεci+ci,εε)=(aibici)+(ai,εbi,εci,ε)ε=ηi+ηi,εε
    Δ(ηi,ηj,ηk)=Δ(ηi,ηj,ηk)+Δ(ηi,ηj,ηk)εε

    where Δ(ηi,ηj,ηk) is a 3×3-determinant,

    Δ(ηi,ηj,ηk)ε=Δ(ηi,ε,ηj,ηk)+Δ(ηi,ηj,ε,ηk)+Δ(ηi,ηj,ηk,ε)

    and

    Δ(ηi,ηj,ηk)ε0=Δ(ηi,ηj,ηk)

    As we can expand, we also get the equalities.

    r3(η0,,η5)=r3(η0,,η5)+r3,ε(η0,,η5)ε

    for Δ(ηi,ηj,ηj)0, the multiplicative inverse of Δ(ηi,ηj,ηk) is 1Δ(ηi,ηj,ηj)Δ(ηi,ηj,ηk)εΔ(ηi,ηj,ηj)2ε and from now on, If simplify the previous equalities, we may use (ηiηjηk) instead of Δ(ηi,ηj,ηk) unless specified.

    r(η0,,η5)=Alt6(η0η1η3)(η1η2η4)(η2η0η5)(η0η1η4)(η1η2η5)(η2η0η3)=Alt6{{(η0η1η3)+(η0η1η3)εε}{(η1η2η4)+(η1η2η4)εε}{(η2η0η5)+(η2η0η5)εε}{(η0η1η4)+(η0η1η4)εε}{(η1η2η5)+(η1η2η5)εε}{(η2η0η3)+(η2η0η3)εε}}

    Simplifying the above and separating the coefficients of ε0 and ε1, we see that the coefficient of ε0 is the triple-ratio of six points (η0,,η5)C6(A3F) and the coefficient of ε is the following:

    r3,ε(η0,,η5)=Alt6{{(η0η1η3)(η1η2η4)(η2η0η5)}ε(η0η1η4)(η1η2η5)(η2η0η3)(η0η1η3)(η1η2η4)(η2η0η5)(η0η1η4)(η1η2η5)(η2η0η3){(η0η1η4)(η1η2η5)(η2η0η3)}ε(η0η1η4)(η1η2η5)(η2η0η3)} (2.8)

    Let F be an algebraically closed field of characteristic 0. Let F[ε]2=F[ε]/ε2 be the truncated polynomial ring (or a ring of dual numbers) for an arbitrary field F. We can define an F×-action in F[ε]2 as follows. For λF×,

    λ:F[ε]2F[ε]2,ϕ+ϕεϕ+λϕε

    we denote this action by , so we use λ(ϕ+ϕε)=ϕ+λϕε.

    The tangent group TB2(F) is defined as a Z-module generated by the combinations [ϕ+ϕε][ϕ]Z[F[ε]2],(ϕ,ϕF): For which we put shorthand ϕ;ϕ]:=[ϕ+ϕε][ϕ] and quotient by the subetaoup generated by the following relation

    ϕ;ϕ]ψ;ψ]+ψϕ;(ψϕ)]1ψ1ϕ;(1ψ1ϕ)]+ϕ(1ψ)ψ(1ϕ);(ϕ(1ψ)ψ(1ϕ))],ϕ,ψ0,1,ϕψ (2.9)

    where

    (ψa)=ϕψϕψϕ2,
    (1ψ1ϕ)=(1ψ)ϕ(1ϕ)ψ(1ϕ)2

    and

    (ϕ(1ψ)ψ(1ϕ))=ψ(1ψ)ϕϕ(1ϕ)ψ(ψ(1ϕ))2

    Remark 2.3. See [1] for a discussion of TB2(F), where the definition of TB2(F) was justified using Lemma 3.1 of [1]

    We give a list of relations in TB2(F) from [1].

    (1) The two-term relation:

    ϕ;ψ]2=1ϕ;ψ]2

    (2) The inversion relation:

    ϕ;ψ]2=1ϕ;ψϕ2]2

    (3) Four-term relation:

    If we use ϕ=ϕ(1ϕ) and ψ=ψ(1ψ) then (2.9) becomes four-term relation (see [1]).

    ϕ;ϕ(1ϕ)]2ψ;ψ(1ψ)]2+ϕψϕ;ψϕ(1ψϕ)]2+(1ϕ)1ψ1ϕ;1ψ1ϕ(11ψ1ϕ)]2=0,

    where ϕ,ψ0,1,ϕψ.

    The following map is an infinitesimal analogue of δ (defined in [4]) and (defined in [1] and [5]), Cathelineau called it the tangential map.

    TB2(F)ε(FF×)(2F)

    with

    ε(ϕ;ψ]2)=(ψϕ(1ϕ)+ψ1ϕϕ)+(ψ1ϕψϕ)

    First term of the complex is in degree one and ε has a degree +1.

    Note that we get the direct sum of two spaces on the right side.

    In this section, we will connect the Grassmannian bicomplex to the tangent to the Bloch-Suslin complex.

    We will use the following notations throughout this section

    Δ(ηi,ηj)ε=Δ(ηi,ε,ηj)+Δ(ηi,ηj,ε)andΔ(ηi,ηj)ε0=Δ(ηi,ηj)

    and we will assume that Δ(ηi,ηj)0 (as we often want to divide by such determinants).

    Let Cm(A2F[ε]2) be the free abelian group generated by the configuration of m points in A2F[ε]2, where A2F[ε]2 is defined as an affine space over F[ε]2. The configurations of m points in A2F[ε]2 are 2-tuples of vectors over F[ε]2 modulo GL2(F[ε]2). In this case, one can write the Grassmannian complex as follows:

    dC5(A2F[ε]2)dC4(A2F[ε]2)dC3(A2F[ε]2)
    d:(η0,,ηm1)mi=0(1)i(η0,,^ηi,,ηm1)

    where ηi=(ϕi+ϕi,εεψi+ψi,εε)=(ϕiψi)+(ϕi,εψi,ε)ε=ηi+ηi,εε and ϕi,ψi,ϕi,ε,ψi,εF, (ϕiψi)(00).

    The diagram below gives the relation between Grassmannian complex and tangent to the Bloch-Suslin complex.

    (4.2a)

    where

    ε:ϕ;ψ]2(ψϕ(1ϕ)+ψ1ϕϕ)+(ψ1ϕψϕ)

    τ20,ε can be written as a sum of two morphisms

    τ(1):C3(A2F[ε]2)FF×

    and

    τ(2):C3(A2F[ε]2)2F

    where

    τ(1)(η0,η1,η2)=Δ(η1,η2)εΔ(η1,η2)Δ(η0,η2)Δ(η0,η1)Δ(η0,η2)εΔ(η0,η2)Δ(η1,η2)Δ(η1,η0)+Δ(η0,η1)εΔ(η0,η1)Δ(η2,η1)Δ(η2,η0)

    and

    τ(2)(η0,η1,η2)=Δ(η0,η1)εΔ(η0,η1)Δ(η1,η2)εΔ(η1,η2)Δ(η0,η1)εΔ(η0,η1)Δ(η0,η2)εΔ(η0,η2)+Δ(η1,η2)εΔ(η1,η2)Δ(η0,η2)εΔ(η0,η2)

    Furthermore, we put

    τ21,ε(η0,,η3)=r(η0,,η3);rε(η0,,η3)]

    where r(η0,,η3) and rε(η0,,η3) are the coefficients of ε0 and ε1 respectively.

    Our maps τ20,ε and τ21,ε are based on ratios of determinants and cross-ratios respectively, so there is enough evidence that they are well-defined. This independence can be seen directly through the definition of maps.

    We will also use shorthand (ηiηj) instead of Δ(ηi,ηj) wherever we find less space to accommodate long expressions.

    Now we calculate,

    1r(η0,,η3)=Δ(η0,η1)Δ(η2,η3)Δ(η0,η2)Δ(η1,η3)=(η0η1)(η2η3)(η0η2)(η1η3)+y(η0η2)2(η1η3)2ε (3.1)

    where

    y=+(η0η2)(η1η3)(η0η1)(η2η3,ε)+(η0η2)(η1η3)(η0η1)(η2,εη3)+(η0η2)(η1η3)(η2η3)(η0η1,ε)+(η0η2)(η1η3)(η2η3)(η0,εη1)(η0η1)(η2η3)(η0η2)(η1η3,ε)(η0η1)(η2η3)(η0η2)(η1,εη3)(η0η1)(η2η3)(η1η3)(η0η2,ε)(η0η1)(η2η3)(η1η3)(η0,εη2)

    Remark 3.1. The F×-action of TB2(F) lifts to an F×-action on C4(A2F[ε]2) in the obvious way:

    The F×-action is defined above for F[ε]2 induces an F×-action in A2F[ε]2 diagonally as

    λ(a+aεεb+bεε)=(a+λaεεb+λbεε)A2F[ε]2,λF×

    Lemma 3.2. The diagram (4.2a) is commutative.

    Proof. The proof follows directly from calculation.

    In the remainder of this section we prove that the following diagram is a bicomplex.

    (4.2b)

    To prove that the above diagram is bicomplex, we will give the next results.

    Proposition 3.3. The map C4(A3F[ε]2)dC3(A2F[ε]2)τ20,ε(FF×)(2F) is zero.

    Proof. Let ωdetV3 be the volume form in three-dimensional vector space V3, i.e., Δ(ηi,ηj,ηk)=ω,ηiηjηk. Then Δ(ηi,,) is a volume form in V3/ηi. Use

    Δ(ηi,ηj,ηk)=Δ(ηi,ηj,ηk)+{Δ(ηi,ηj,ηk)ε}ε

    where

    Δ(ηi,ηj,ηk)ε=Δ(ηi,ε,ηj,ηk)+Δ(ηi,ηj,ε,ηk)+Δ(ηi,ηj,ηk,ε)

    We can directly compute τ20,εd which gives zero.

    The following result is very important for proving Theorem 3.7. Through this result we are able to see the projected-five term relation for TB2(F).

    Lemma 3.4. Let x0,,x4P2F[ε]2 be 5 points in generic position. Then

    4i=0(1)ir(xi|x0,,ˆxi,,x4);rε(xi|x0,,ˆxi,,x4)]=0TB2(F), (3.2)

    where xi=xi+xiε and xi,xiP2F

    r(xi|x0,,ˆxi,,x4)=r(xi|x0,,ˆxi,,x4)+rε(xi|x0,,ˆxi,,x4)ε,

    Proof. Consider five points y0,,y4P1F in generic position. We can write the five-term relation in terms of cross-ratios in B2(F) as (see Proposition 4.5 (2)b in [2]):

    4i=0(1)i[r(y0,,ˆyi,,y4)]2=0

    These five points depend on 2 parameters modulo the action of PGL2(F), whose action on P1F is 3-fold transitive. So we can express these five points with two variables modulo this action as, we can put

    (y0,,y4)=((10),(01),(11),(1ϕ1),(1ψ1)).

    Then we can get the five-term relation in two variables (by using inversion relation in the last two terms).

    [ϕ]2[ψ]2+[ψϕ]2+[1ϕ1ψ]2[11ϕ11b]2=0.

    Now we consider five points y0,,y4P1F[ε]2, in generic position, where yi=yi+yiε for yi,yiP1F. A generic 2×2 matrix in PGL2(F[ε]2) depends on 6=2(2×2)2(1) parameters, while each point in P1F[ε]2 depends on 2 parameters, so these five points in P1F[ε]2 modulo the action of PGL2(F[ε]2) have 4 parameters. Now we can express them by using four variables we choose:

    (y0,,y4)=((10),(01),(11),(1ϕϕϕ2ε1),(1ψψψ2ε1)).

    We calculate all possible determinants which are the following:

    Δ(y0,y1)=Δ(y0,y2)=Δ(y0,y3)=Δ(y0,y4)=1,Δ(y1,y2)=1,Δ(y1,y3)=1ϕ,Δ(y1,y4)=1ψ,Δ(y2,y3)=11ϕ,Δ(y2,y4)=11ψΔ(y0,y1)ε=Δ(y0,y2)ε=Δ(y0,y3)ε=Δ(y0,y4)ε=Δ(y1,y2)ε=0Δ(y1,y3)ε=Δ(y2,y3)ε=ϕϕ2,Δ(y1,y4)ε=Δ(y2,y4)ε=ψψ2

    For y0,,y4P1F[ε]2, we can write the following expression in TB2(F)

    4i=0(1)ir(y0,,ˆyi,,y4);rε(y0,,ˆyi,,y4)]2

    If we expand the above expression and substitute all the determinants in it, we will get the following expression in two variables.

    ϕ;ϕ]2ψ;ψ]2+ψϕ;ϕψϕψϕ2]21ψ1ϕ;(1ψ)ϕ(1ϕ)ψ(1ϕ)2]2+ϕ(1ψ)ψ(1ϕ);ψ(1ψ)ϕϕ(1ϕ)ψ(ψ(1ϕ))2]2

    From (3.2) it is clear that the above is the LHS of the five-term relation in TB2(F). We first show underneath, that this claim is valid, then later we reduce it to the five-term relation.

    Consider x0,,x4P2F in generic position. These five points also depend on 2 parameters modulo the action of PGL2(F), so we can express these five points in terms of two variables by the following choice:

    (x0,,x4)=((100),(010),(001),(111),(1ψ1ϕ1))

    We compute all possible 3×3 determinants of the above and put them in the expansion of the following:

    4i=0(1)i[r(xi|x0,,ˆxi,,x4)]2B2(F),

    we get the following expression in two variables

    [ϕ]2ψ]2+[ψϕ]2+[1ϕ1ψ]2[11ϕ11ψ]2,

    clearly the above is the LHS of one version of five-term relation in B2(F).

    Since by assumption x0,,x4P2F[ε]2 are 5 points in generic position, we can express them as modulo the action of PGL3(F[ε]2) into 4 parameters, then we can choose these points in terms of four variables in the following way:

    (x0,,x4)=((100),(010),(001),(111),(1bbb2ε1ϕϕϕ2ε1))

    We compute all possible 3×3 determinants and substitute them in an expansion of the following:

    4i=0(1)ir(xi|x0,,ˆxi,,x4);rε(xi|x0,,ˆxi,,x4)]2TB2(F),

    we get

    ϕ;ϕ]2ψ;ψ]2+ψϕ;ϕψϕψϕ2]21ψ1ϕ;(1ψ)ϕ(1ϕ)ψ(1ϕ)2]2+ϕ(1ψ)ψ(1ϕ);ψ(1ψ)ϕϕ(1ϕ)ψ(ψ(1ϕ))2]2

    which is the five-term expression in TB2(F) up to invoking the inversion relation for the last two terms, which also holds in TB2(F).

    Lemma 3.4 indicates that we now have the projected five-term relation for TB2(F) and this relation will help us to prove the commutative diagram for weight n=3 in the tangential case.

    Proposition 3.5. The map C5(A3F[ε]2)dC4(A2F[ε]2)τ21,εTB2(F) is zero.

    Proof. We can directly calculate τ21εd.

    τ21,εd(η0,,η4)=τ21,ε(4i=0(1)i(ηi|η0,,ˆηi,,η4))=4i=0(1)ir(ηi|η0,,ˆηi,η4);rε(ηi|η0,,ˆηi,,η4)]2 (3.3)

    The above is the projected five term relation in TB2(F) by Lemma 3.4.

    Theorem 3.2 shows that the diagram (4.2a) is commutative and Propositions 3.3 and 3.5 shows that we have formed a bicomplex between the Grassmannian complex and Cathelineau's tangent complex.

    We have already discussed the tangent group (or Z-module) TB2(F) over F[ε]2 in §3.1. In this section we will discuss group TB3(F) and its functional equations and will connect Grassmannian complex and tangential complex to Goncharov complex.

    The Z-module TB3(F) over F[ε]2 is defined as the group generated by:

    a;b]=[a+bε][a]Z[F[ε]2],a,bF,a0,1

    and quotient by the kernel of the following map

    ε,3:Z[F[ε]2]TB2(F)F×FB2(F),a;b]a;b]2a+ba[a]2

    Now we say that a;b]3TB3(F)Z[F[ε]2]/kerε,3.

    We have the following relations which are satisfied in TB3(F).

    (1) The three-term relation.

    1a;(1a)ε]3a;aε]311a;(11a)ε]3=0TB3(F)

    (2) The inversion relation

    a;aε]3=1a;(1a)ε]3

    (3) The Cathelineau 22-term relation ([2])

    This relation J(a,b,c) for the indeterminates a,b,c can be written in this way:

    J(a,b,c)=[[a,c]][[b,c]]+a[[ba,c]]+(1a)[[1b1a,c]], (3.4)

    where

    [[a,b]]=(ba)τ(a,b)+1b1aσ(a)+1a1bσ(b),

    while τ(a,b) is defined via five term relation and -action. We take xi;xi,ε]3 with coefficient 11xi which is handled by -action.

    τ(a,b)=a;aε11a]3b;bε11b]3+ba;(ba)ε1ab]31b1a;(1b1a)ε1ba]3a(1b)b(1a);(a(1b)b(1a))ε1ba]3

    and

    σ(a)=a;aεa]3+1a;(1a)ε(1a)]3.

    Then we can calculate Cathelineau's 22-term expression by substituting all values in (3.4).

    J(a,b,c)=a;aεc]3b;bεc]3+c;cε(ab+1)]3+1a;(1a)ε(1c)]31b;(1b)ε(1c)]3+1c;(1c)ε(ba)]3ca;(ca)ε]3+cb;(cb)ε]3+ba;(ba)εc]31c1a;(1c1a)ε]3+1c1b;(1c1b)ε]3+1b1a;(1b1a)εc]3+a(1c)c(1a);(a(1c)c(1a))ε]3cab;(cab)ε]3b(1c)c(1b);(b(1c)c(1b))ε]3+aba;(aba)ε(1c)]3+ba1a;(ba1a)ε(1c)]3+c(1a)1b;(c(1a)1b)ε]3(1c)aab;((1c)aab)ε]3(1c)(1a)ba;((1c)(1a)ba)ε]3+(1c)bc(ab);((1c)bc(ab))ε]3+(1c)(1b)c(ba);((1c)(1b)c(ba))ε]3 (3.5)

    For the special condition aε=a(1a), bε=b(1b) and cε=c(1c), this 22-term expression becomes zero in TB3(F).

    One can write the following complex for TB3(F).

    TB3(F)εTB2(F)F×FB2(F)ε(F2F×)(3F)

    In this section, we will introduce morphisms between the Grassmannian complex and the tangent to Goncharov's complex for weight n=3. Consider the following diagram

    (4.3a)

    Here we define the projected cross-ratio

    r(η0|η1,η2,η3,η4)=Δ(η0,η1,η4)Δ(η0,η2,η3)Δ(η0,η1,η3)Δ(η0,η2,η4)

    which can be further simplifed to

    r(η0|η1,η2,η3,η4)=r(η0|η1,η2,η3,η4)+rε(η0|η1,η2,η3,η4)ε

    where

    r(η0|η1,η2,η3,η4)=Δ(η0,η1,η4)Δ(η0,η2,η3)Δ(η0,η1,η3)Δ(η0,η2,η4)
    rε(η0|η1,η2,η3,η4)=uΔ(η0,η1,η3)2Δ(η0,η2,η4)2
    u=Δ(η0,η1,η4)Δ(η0,η2,η3){Δ(η0,η1,η3)Δ(η0,η2,η4)ε+Δ(η0,η2,η4)Δ(η0,η1,η3)ε}+Δ(η0,η1,η3)Δ(η0,η2,η4){Δ(η0,η1,η4)Δ(η0,η2,η3)ε+Δ(η0,η2,η3)Δ(η0,η1,η4)ε}

    where the morphisms between the two complexes are defined as follows:

    τ30,ε(η0,,η3)=3i=0(1)i(Δ(η0,,ˆηi,,η3)εΔ(η0,,ˆηi,,η3)Δ(η0,,ˆηi+1,,η3)Δ(η0,,ˆηi+2,,η3)Δ(η0,,ˆηi+3,,η3)Δ(η0,,ˆηi+2,,η3)+3j=0jiΔ(η0,,ˆηj,,η3)εΔ(η0,,ˆηj,,η3)),imod4,
    τ31,ε(η0,,η4)=134i=0(1)i(r(ηi|η0,,ˆηi,,η4);rε(ηi|η0,,^ηi,,η4)]2ijΔ(ˆηi,ˆηj)+4j=0ji(Δ(η0,,ˆηi,,ˆηj,,η4)εΔ(η0,,ˆηi,,ˆηj,,η4))[r(ηi|η0,,ˆηi,,η4)]2)

    and

    τ32ε(η0,,η5)=245Alt6r3(η0,,η5);r3,ε(η0,,η5)]3

    where

    r3(η0,,η5)=(η0η1η3)(η1η2η4)(η2η0η5)(η0η1η4)(η1η2η5)(η2η0η3)

    and

    r3,ε(η0,,η5)={(η0η1η3)(η1η2η4)(η2η0η5)}ε(η0η1η4)(η1η2η5)(η2η0η3)(η0η1η3)(η1η2η4)(η2η0η5)(η0η1η4)(η1η2η5)(η2η0η3){(η0η1η4)(η1η2η5)(η2η0η3)}ε(η0η1η4)(η1η2η5)(η2η0η3) (3.6)

    the map ε is defined as

    ε(a;b]2c+x[y]2)=(b1aacba(1a)c+x(1y)y)+(b1abax)

    and

    ε(a;b]3)=a;b]2a+ba[a]2

    Theorem 3.6. Diagram (4.3a), i.e.,

    is commutative, i.e., τ30,εd=ετ31,ε

    Proof. First we divide the map τ30,ε=τ(1)+τ(2) then calculate τ(1)d(η0,,η4)

    τ(1)d(η0,,η4)=τ30,ε(4i=0(1)i(η0,,ˆηi,,η4))
    =~Alt(01234)(3i=0(1)i(Δ(η0,,ˆηi,,η3)εΔ(η0,,ˆηi,,η3)Δ(η0,,ˆηi+1,,η3)Δ(η0,,ˆηi+2,,η3)Δ(η0,,ˆηi+3,,η3)Δ(η0,,ˆηi+2,,η3)),imod4) (3.7)

    Now, we expand the inner sum that contains 12 terms and pass them through the this alternation to the inner sum, gives us 60 different terms overall. We collect terms involving the same Δ(ηi,ηj,ηk)Δ(ηi,ηj,ηk) together for calculation purposes. On the other hand the second part of the map is:

    τ(1)d(η0,,η4)=~Alt(01234)(3i=0(1)i3j=0jiΔ(η0,,ˆηj,,η3)εΔ(η0,,ˆηj,,η3)) (3.8)

    The other side of the proof requires tedious computations. For the calculation of ετ31,ε we will use the short hand (ηiηjηk)ε for Δ(ηi,ηj,ηk)ε and (ηiηjηk) for Δ((ηi,ηj,ηk). First we write ετ31,ε(η0,,η4) by using the definitions above.

    ετ31,ε(η0,,η4)=ε(134i=0(1)i(r(ηi|η0,,ˆηi,,η4);rε(ηi|η0,,^ηi,,η4)]2ijΔ(ˆηi,ˆηj)+4j=0ji(Δ(η0,,ˆηi,,ˆηj,,η4)εΔ(η0,,ˆηi,,ˆηj,,η4))[r(ηi|η0,,ˆηi,,η4)]2))

    then we divide ε=(1)+(2). The first part (1)τ31,ε(η0,,η4) is

    =134i=0(1)i(rε(ηi|η0,,ˆηi,,η4)1r(ηi|η0,,ˆηi,,η4)r(ηi|η0,,ˆηi,,η4)ij(ˆηi,ˆηj)rε(ηi|η0,,ˆηi,,η4)r(ηi|η0,,ˆηi,,η4)(1r(ηi|η0,,ˆηi,,η4))ij(ˆηi,ˆηj)+4j=0ji(Δ(η0,,ˆηi,,ˆηj,,η4)εΔ(η0,,ˆηi,,ˆηj,,η4))(1r(ηi|η0,,ˆηi,,η4))r(ηi|η0,,ˆηi,,η4)) (3.9)

    The second part (2)τ31,ε(η0,,η4) is

    =134i=0(1)i(rε(ηi|η0,,ˆηi,,η4)r(ηi|η0,,ˆηi,,η4)rε(ηi|η0,,ˆηi,,η4)1r(ηi|η0,,ˆηi,,η4)4j=0ji(Δ(η0,,ˆηi,,ˆηj,,η4)εΔ(η0,,ˆηi,,ˆηj,,η4))) (3.10)

    then we calculate bεa and bε1a. i.e., all the values of the form rε(η0|η1,η2,η3,η4)r(η0|η1,η2,η3,η4) and rε(η0|η1,η2,η3,η4)1r(η0|η1,η2,η3,η4). By using formula (2.6) we get

    rε(η0|η1,η2,η3,η4)r(η0|η1,η2,η3,η4)=(η0η1η4)ε(η0η1η4)+(η0η2η3)ε(η0η2η3)(η0η2η4)ε(η0η2η4)(η0η1η3)ε(η0η1η3)

    Similarly, we can find this ratio for each value of i=0,,4. Now use formula (2.6) with the identities (2.1) and (2.3);

    rε(η0|η1,η2,η3,η4)1r(η0|η1,η2,η3,η4)=(η0η2η4)ε(η0η2η4)+(η0η1η3)ε(η0η1η3)(η0η3η4)ε(η0η3η4)(η0η1η2)ε(η0η1η2)

    After calculating all these values, expand the sums (3.9) and (3.10) and put all values that we have calculated above. For instance, let us calculate (3.9). In this sum we have a large number of terms, so we group them in a suitable way. First collect all the terms involving (η0η1η2)ε(η0η1η2), we find that there are 6 different terms with coefficient -3 involving (η0η1η2)ε(η0η1η2)

    3(η0η1η2)ε(η0η1η2)((η0η1η3)(η1η2η3)+(η0η2η4)(η1η2η3)+(η0η1η4)(η0η2η4)(η0η1η3)(η0η2η3)(η0η1η4)(η1η2η4)(η0η2η3)(η1η2η3))

    There are exactly 10 possible terms of (ηiηjηk)ε(ηiηjηk). Compute all of them individually. We will see that each will have the coefficient 3 that will be cancelled by 13 in (3.9) and then combine 60 different terms with 6 in a group of the same (ηiηjηk)ε(ηiηjηk), write in the sum form then we will note that it will be the same as (3.7).

    Computation for the second part is relatively easy and direct. We need to put all values of the form rε(η0|η1,η2,η3,η4)r(η0|η1,η2,η3,η4) and rε(η0|η1,η2,η3,η4)1r(η0|η1,η2,η3,η4) in (3.10), expand the sums, use aa=0 modulo 2 torsion. Here we will have simplified result which can be recombined in the sum notation which will be the same as (3.8).

    Theorem 3.7. The following diagram (4.3a), i.e.,

    is commutative i.e., τ32,εε=dτ31,ε

    Proof. The map τ32,ε gives 720 terms and due to symmetry (cyclic and inverse) we find 120 different ones (up to the inverse). By definition, we have

    τ32,ε(η0,,η5)=245Alt6r3(η0,,η5);r3,ε(η0,,η5)]3

    For convenience, and similar to our previous conventions, we will abbreviate our notation by dropping Δ and commas.

    ετ32ε(η0η5)=245Alt6{r3(η0η5);r3,ε(η0η5)]2r3(η0η5)+r3,ε(η0η5)r3(η0η5)[r3(η0η5)]2} (3.11)

    We need to compute the value of r3,ε(η0η5)r3(η0η5) which is

    =(η0η1η3)ε(η0η1η3)+(η1η2η4)ε(η1η2η4)+(η2η0η5)ε(η2η0η5)(η0η1η4)ε(η0η1η4)(η1η2η5)ε(η1η2η5)(η2η0η3)ε(η2η0η3)

    Formula (3.11) can also be written as

    =245Alt6{r3(η0η5);r3,ε(η0η5)]2(η0η1η3)(η1η2η4)(η2η0η5)(η0η1η4)(η1η2η5)(η2η0η3)+((η0η1η3)ε(η0η1η3)+(η1η2η4)ε(η1η2η4)+(η2η0η5)ε(η2η0η5)(η0η1η4)ε(η0η1η4)(η1η2η5)ε(η1η2η5)(η2η0η3)ε(η2η0η3))[r3(η0η5)]2}

    We will consider here only the first part of the above relation.

    245Alt6{r3(η0η5);r3,ε(η0η5)]2(η0η1η3)(η1η2η4)(η2η0η5)(η0η1η4)(η1η2η5)(η2η0η3)

    Further,

    =Alt6{r3(η0η5);r3,ε(η0η5)]2(η0η1η3)}+Alt6{r3(η0η5);r3,ε(η0η5)]2(η1η2η4)}+Alt6{r3(η0η5);r3,ε(η0η5)]2(η2η0η5)}Alt6{r3(η0η5);r3,ε(η0η5)]2(η0η1η4)}Alt6{r3(η0η5);r3,ε(η0η5)]2(η1η2η5)}Alt6{r3(η0η5);r3,ε(η0η5)]2(η2η0η3)} (3.12)

    We use the even cycle (η0η1η2)(η3η4η5) (or (η0η1η2)(η3η4η5)) to obtain

    Alt6{r3(η0η1η2η3η4η5);r3,ε(η0η1η2η3η4η5)]2(η0η1η3)}=Alt6{r3(η1η2η0η4η5η3);r3,ε(η1η2η0η4η5η3)]2(η1η2η4)}

    We can also use here the symmetry

    r3(η0η1η2η3η4η5);r3,ε(η0η1η2η3η4η5)]2=r3(η1η2η0η4η5η3);r3,ε(η1η2η0η4η5η3)]2

    since

    r3,ε(η0η1η2η3η4η5)=r3,ε(η1η2η0η4η5η3)precisely both have the same factors

    and similar for the others as well so that (3.12) will be

    =215Alt6{r3(η0η1η2η3η4η5);r3,ε(η0η1η2η3η4η5)]2(η0η1η3)r3(η0η1η2η3η4η5);r3,ε(η0η1η2η3η4η5)]2(η0η1η4)}

    If we apply the odd permutation (η3η4) (or (η3η4)), then we have

    =2152Alt6{r3(η0η1η2η3η4η5);r3,ε(η0η1η2η3η4η5)]2(η0η1η3)}

    Again apply the odd permutation (η0η3) (or (η0η3))

    =215Alt6{r3(η0η1η2η3η4η5);r3,ε(η0η1η2η3η4η5)]2(η0η1η3)r3(η3η1η2η0η4η5);r3,ε(η3η1η2η0η4η5)]2(η3η1η0)}

    but up to 2-torsion, which we ignore here, we have (η0η1η3)=(η3η1η0) and then the above will become

    =215Alt6{(r3(η0η1η2η3η4η5);r3,ε(η0η1η2η3η4η5)]2r3(η3η1η2η0η4η5);r3,ε(η3η1η2η0η4η5)]2)(η0η1η3)} (3.13)

    Recall from the triple-ratio

    r3(η0η1η2η3η4η5)=(η0η1η3)(η1η2η4)(η2η0η5)(η0η1η4)(η1η2η5)(η2η0η3)

    can be expressed as the ratio of two projected cross-ratios.

    We will see here that r3,ε(η0η1η2η3η4η5) can also be converted into the ratio of two first order cross-ratios.

    Let a and b be two projected cross-ratios whose ratio is the triple-ratio

    r3(η0η1η2η3η4η5)=(η0η1η3)(η1η2η4)(η2η0η5)(η0η1η4)(η1η2η5)(η2η0η3)

    then r3,ε(η0η1η2η3η4η5) will be written as (ab)ε. Since we can also write as

    r3(η0η1η2η3η4η5)=r3(η0η1η2η3η4η5)+r3,ε(η0η1η2η3η4η5)ε

    or

    r3(η0η1η2η3η4η5)=r3(η0η1η2η3η4η5)+(r3(η0η1η2η3η4η5))εε

    we get

    r3,ε(η0η1η2η3η4η5)=((η0η1η3)(η1η2η4)(η2η0η5)(η0η1η4)(η1η2η5)(η2η0η3))ε

    Now it is clear that r3,ε(η0η1η2η3η4η5) can also be written as the ratio or product of two projected cross-ratios. There are exactly three ways to write it (projected by (η0 and η1), (η1 and η2) and (η0 and η2)) but we will use here η1 and η2. The last expression can be written as

    r3,ε(η0η1η2η3η4η5)=(r(η2|η1η0η5η3)r(η1|η0η2η3η4))ε

    and (3.13) can be written as

    =215Alt6{r(η2|η1η0η5η3)r(η1|η0η2η3η4);(r(η2|η1η0η5η3)r(η1|η0η2η3η4))ε]2(η0η1η3)r(η2|η1η3η5η0)r(η1|η3η2η0η4);(r(η2|η1η3η5η0)r(η1|η3η2η0η4))ε]2(η0η1η3)}

    Applying five-term relations in TB2(F) which are analogous to the one in (2.9).

    =215Alt6{(r(η2|η1η0η5η3);rε(η2|η1η0η5η3)]2r(η1|η0η2η3η4);rε(η1|η0η2η3η4)]2r(η2|η1η5η3η0)r(η1|η0η3η4η2);(r(η2|η1η5η3η0)r(η1|η0η3η4η2))ε]2)(η0η1η3)} (3.14)

    For each individual determinant, e.g., (η0η1η3) will have three terms. First consider the third term of (3.14)

    215Alt6{r(η2|η1η5η3η0)r(η1|η0η3η4η2);(r(η2|η1η5η3η0)r(η1|η0η3η4η2))ε]2(η0η1η3)}=215Alt6{136Alt(η0η1η3)(η2η4η5)(r(η2|η1η5η3η0)r(η1|η0η3η4η2);(r(η2|η1η5η3η0)r(η1|η0η3η4η2))ε]2(η0η1η3))}

    We need a subetaoup in S6 which fixes (η0η1η3) as a determinant i.e., (η0η1η3)(η3η1η0)(η3η0η1)

    Here in this case S3 permuting {η0,η1,η3} and another one permuting {η2,η4,η5} i.e., S3×S3. Now consider

    Alt(η0η1η3)(η2η4η5){r(η2|η1η5η3η0)r(η1|η0η3η4η2);(r(η2|η1η5η3η0)r(η1|η0η3η4η2))ε]2(η0η1η3)}=Alt(η0η1η3)(η2η4η5){(η2η5η3)(η1η0η4)(η2η5η0)(η1η3η4);((η2η5η3)(η1η0η4)(η2η5η0)(η1η3η4))ε]2(η0η1η3)}

    By using the odd permutation (η2η5) the above becomes zero.

    then (3.14) becomes

    =215Alt6{(r(η2|η1η0η5η3);rε(η2|η1η0η5η3)]2r(η1|η0η2η3η4);rε(η1|η0η2η3η4)]2)(η0η1η3)} (3.15)

    Consider the first term now,

    215Alt6{r(η2|η1η0η5η3);rε(η2|η1η0η5η3)]2(η0η1η3)}
    =215Alt6{136Alt(η0η1η3)(η2η4η5){r(η2|η1η0η5η3);rε(η2|η1η0η5η3)]2(η0η1η3)}}

    The permutation (η0η2η3) does not have any role because the ratio is projected by 2. So, it will be reduced to S3.

    =215Alt6{16Alt(η2η4η5){r(η2|η1η0η5η3);rε(η2|η1η0η5η3)]2(η0η1η3)}}

    Write all possible inner alternation, then

    =145Alt6{(r(η4|η1η0η2η3);rε(η4|η1η0η2η3)]2r(η2|η1η0η4η3);rε(η2|η1η0η4η3)]2+r(η5|η1η0η4η3);rε(η5|η1η0η4η3)]2r(η4|η1η0η5η3);rε(η4|η1η0η5η3)]2+r(η2|η1η0η5η3);rε(η2|η1η0η5η3)]2r(η5|η1η0η2η3);rε(η5|η1η0η2η3)]2)(η0η1η3)}

    Now we can use projected five-term relation in here,

    (3.16)

    The second term of the relation (3.15) can also be written as

    (3.16) can be combined with the above so we get

    (3.17)

    Use the permutation to get

    The Bloch group also holds the five-term relation, thus we write the following:

    (3.18)

    Now go to the other side. Map can also be written in the alternation sum form

    Compute and apply cycle for and then expand Alt from the definition of ;

    Use the odd permutation , then

    Finally use the two-term relation in and the Bloch group to get the required sign. The final answer will be the same as (3.18)

    There are some more related results.

    Proposition 3.18. The map is zero.

    Proof. The proof of this is obtained directly by calculation. Let where

    Let be the volume formed in four-dimensional vector space, and be the volume form in .

    (3.19)

    First, we expand inner sum that gives us 12 different terms after simplification. By applying alternation sum, we get 60 terms and there is direct cancellation which leads to zero. Now consider the second coordinate, which gives us

    Again if we expand the inner sum, then we get only four different terms, but after the application of alternation we get zero.

    As an analogy of Proposition 3.8 in higher weight, we present the following result.

    Proposition 3.9. The map is zero, where

    Proof. Let . We have

    Now use the definition of alternation to represent this sum then we have

    (3.20)

    Expanding the inner sum gives us number of terms. Expand again by using the properties of wedge that gives terms. Applying the alternation sum on that, gives us terms, so there are sets each consisting terms and each term in term has sets of terms which cancel off set by set.

    Now expand the inner sum in the second term of (3.20) that gives terms and then apply the alternation sum which gives sets of terms, we now find cancellation in the expansion of sum accordingly, which gives a zero as well.

    Many studies have been done on Scissor's congruence and Bloch's groups. Bringing geometry of configurations in Bloch's and Goncharov's groups plays a vital role in proving Zagier's conjucutre for weights . In this article, we introduced the tangent to Goncharov's complex and view them by means of geometric configurations. This leads to the idea at the higher orders of tangent groups.

    Theorem 3.6 proves the commutativity of the right hand side square of the diagram (4.3a) and Theorem 3.7 shows the commutativity of the left hand square of the diagram (4.3a).

    This article consists on major part of the author's Doctoral thesis completed at University of Durham, UK. The author would like to thank to his advisor Dr. Herbert Gangl. The author would also like to thank to Spencer Bloch for his valuable suggestions.

    The author declares no conflict of interest in this paper.


    Acknowledgments



    This study was partly funded by the PhD funds and the HiF funds of Bielefeld University of Applied Sciences.

    Conflict of interest



    All authors declare no conflicts of interest in this paper.

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