Construction of lncRNA regulatory networks reveal the key lncRNAs associated with Pituitary adenomas progression

Yonghua Xue; Yiqin Ge; Yonghua Xue; Yiqin Ge

doi:10.3934/mbe.2020113

Mathematical Biosciences and Engineering

2020, Volume 17, Issue 3: 2138-2149. doi: 10.3934/mbe.2020113

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Construction of lncRNA regulatory networks reveal the key lncRNAs associated with Pituitary adenomas progression

Yonghua Xue ,
Yiqin Ge ^,

Department of Neurosurgery, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200062, China

Received: 20 May 2019 Accepted: 28 November 2019 Published: 06 January 2020

Pituitary adenomas (PA) is one of the most frequent types of intracranial neoplasms. Long noncoding RNAs (lncRNAs) played important roles in the progression of human cancers, including PA. However, the roles of lncRNAs in PA remained to be further investigated. We performed analysis of GSE26966 dataset to identify differently expressed lncRNAs in PA. Co-expression network, lncRNA-RNA binding proteins network, and competing endogenous RNA networks were constructed. Moreover, we performed RT-qPCR assay to validate four key lncRNAs expression in PA. This study identified differently expressed mRNAs and lncRNAs by using GSE26966 database. Furthermore, we constructed lncRNA-mRNA co-expression, lncRNA-RBP interaction and ceRNA networks. Bioinformatics analysis showed these lncRNAs were involved in regulating mechanical stimulus, gene expression, JAK-STAT cascade, cell cycle arrest, FoxO signaling, HIF-1 signaling, Insulin signaling, Oxytocin signaling, and MAPK signaling. We also showed KCNQ1OT1, SNHG7, MEG3, and SNHG5 were down-regulated in PA. Our findings could provide a novel insight to understand the mechanisms of lncRNAs underlying PA pathogenesis and identify new biomarkers for PA.
- long non-coding RNAs,
- Pituitary adenomas,
- biomarker, co-expression,
- competing endogenous RNA
Citation: Yonghua Xue, Yiqin Ge. Construction of lncRNA regulatory networks reveal the key lncRNAs associated with Pituitary adenomas progression[J]. Mathematical Biosciences and Engineering, 2020, 17(3): 2138-2149. doi: 10.3934/mbe.2020113

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Abstract

Pituitary adenomas (PA) is one of the most frequent types of intracranial neoplasms. Long noncoding RNAs (lncRNAs) played important roles in the progression of human cancers, including PA. However, the roles of lncRNAs in PA remained to be further investigated. We performed analysis of GSE26966 dataset to identify differently expressed lncRNAs in PA. Co-expression network, lncRNA-RNA binding proteins network, and competing endogenous RNA networks were constructed. Moreover, we performed RT-qPCR assay to validate four key lncRNAs expression in PA. This study identified differently expressed mRNAs and lncRNAs by using GSE26966 database. Furthermore, we constructed lncRNA-mRNA co-expression, lncRNA-RBP interaction and ceRNA networks. Bioinformatics analysis showed these lncRNAs were involved in regulating mechanical stimulus, gene expression, JAK-STAT cascade, cell cycle arrest, FoxO signaling, HIF-1 signaling, Insulin signaling, Oxytocin signaling, and MAPK signaling. We also showed KCNQ1OT1, SNHG7, MEG3, and SNHG5 were down-regulated in PA. Our findings could provide a novel insight to understand the mechanisms of lncRNAs underlying PA pathogenesis and identify new biomarkers for PA.

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