Comparative genome analysis of the first <i>Listeria monocytogenes</i> core genome multi-locus sequence types CT2050 AND CT2051 strains with their close relatives

Ogueri Nwaiwu; Ogueri Nwaiwu

doi:10.3934/microbiol.2022006

AIMS Microbiology

2022, Volume 8, Issue 1: 61-72. doi: 10.3934/microbiol.2022006

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Comparative genome analysis of the first Listeria monocytogenes core genome multi-locus sequence types CT2050 AND CT2051 strains with their close relatives

Ogueri Nwaiwu ^,

School of Biosciences, University of Nottingham, Sutton Bonington Campus, LE12 5RD

Received: 08 January 2022 Revised: 02 March 2022 Accepted: 15 March 2022 Published: 21 March 2022

Genome sequences of the three strains of L. monocytogenes, which are the first core genome multi-locus sequence types (cgMLST) 2050 and 2051 were reviewed and compared with 21 close relatives and reference genomes. Using a pan-genomic approach to analyse whole genome sequences, it was found that the strains consisted of approximately 2200 shared genes and a much greater pool of genes present as an accessory genome. An unknown transmissible sequence of approximately 91 kb harbouring bacitracin resistance genes found in strain LmNG2 (1/2b) was revealed to be an Inc18 plasmid. The CT2051, strain LmNG3 (1/2a) haboured more unique genes (252 vs 230) than the well-known reference strain LmEGD-e (1/2a). More studies to monitor new strains can help reduce food-borne outbreaks.
- Listeria monocytogenes,
- pan-genome,
- antimicrobial resistance genes,
- plasmid,
- bacitracin genes,
- genomics
Citation: Ogueri Nwaiwu. Comparative genome analysis of the first Listeria monocytogenes core genome multi-locus sequence types CT2050 AND CT2051 strains with their close relatives[J]. AIMS Microbiology, 2022, 8(1): 61-72. doi: 10.3934/microbiol.2022006

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Abstract

Genome sequences of the three strains of L. monocytogenes, which are the first core genome multi-locus sequence types (cgMLST) 2050 and 2051 were reviewed and compared with 21 close relatives and reference genomes. Using a pan-genomic approach to analyse whole genome sequences, it was found that the strains consisted of approximately 2200 shared genes and a much greater pool of genes present as an accessory genome. An unknown transmissible sequence of approximately 91 kb harbouring bacitracin resistance genes found in strain LmNG2 (1/2b) was revealed to be an Inc18 plasmid. The CT2051, strain LmNG3 (1/2a) haboured more unique genes (252 vs 230) than the well-known reference strain LmEGD-e (1/2a). More studies to monitor new strains can help reduce food-borne outbreaks.

Conflict of interest

The author declares no conflict of interest.

Author contributions

N/a

References

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