<i>Hypericum alpestre</i> extract affects the activity of the key antioxidant enzymes in microglial BV-2 cellular models

Mikayel Ginovyan; Pierre Andreoletti; Mustapha Cherkaoui-Malki; Naira Sahakyan; Mikayel Ginovyan; Pierre Andreoletti; Mustapha Cherkaoui-Malki; Naira Sahakyan

doi:10.3934/biophy.2022014

AIMS Biophysics

2022, Volume 9, Issue 2: 161-171. doi: 10.3934/biophy.2022014

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Research article

Hypericum alpestre extract affects the activity of the key antioxidant enzymes in microglial BV-2 cellular models

1.
Department of Biochemistry, Microbiology & Biotechnology, Yerevan State University, 1 A. Manoogian Str., 0025 Yerevan, Armenia
2.
Research Institute of Biology, Yerevan State University, 1 A. Manoogian Str., 0025 Yerevan, Armenia
3.
Laboratoire Bio-PeroxIL EA7270, University of Bourgogne Franche-Comté, 21000 Dijon, France

Received: 11 May 2022 Revised: 09 June 2022 Accepted: 15 June 2022 Published: 20 June 2022

In the presented work, we aimed to investigate the antioxidant and possible neuroprotective capacity of extract of the aerial parts of Hypericum alpestre, found in high altitude Armenian landscape. The neuroprotective activity was evaluated using BV-2 wild type (WT) cells and acyl-CoA oxidase 1 (ACOX1) deficient (Acox1^-/-) microglial cell lines. In the chemical-based tests, H. alpestre extract showed high antioxidant activity, which was maintained even after heat treatment at 121 °C for 30 min. MTT test showed that the sub-cytotoxic concentration of investigated extracts for both microglial cell lines was 40 µg/mL. There were no significant changes in catalase activity during all period of treatment in both cell lines, meanwhile, SOD activity increased (up to 30%) in WT cells during the 48 h treatment. Increase of SOD activity (up to 50%) in Acox^-/- cells was observed under the 24 h treatment. Significant modulation in activity of palmitoyl-CoA oxidase 1 was noticed only during the 48 h treatment of WT microglial cells. These results evidenced the pro-oxidant activity of the investigated extract. This finding can serve as a basis for further evaluation of plant extracts influence on cancer cell lines.
- plant extract,
- Armenian flora,
- total phenolic content,
- Hypericaceae,
- antioxidant enzymes,
- microglia
Citation: Mikayel Ginovyan, Pierre Andreoletti, Mustapha Cherkaoui-Malki, Naira Sahakyan. Hypericum alpestre extract affects the activity of the key antioxidant enzymes in microglial BV-2 cellular models[J]. AIMS Biophysics, 2022, 9(2): 161-171. doi: 10.3934/biophy.2022014

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Abstract

In the presented work, we aimed to investigate the antioxidant and possible neuroprotective capacity of extract of the aerial parts of Hypericum alpestre, found in high altitude Armenian landscape. The neuroprotective activity was evaluated using BV-2 wild type (WT) cells and acyl-CoA oxidase 1 (ACOX1) deficient (Acox1^-/-) microglial cell lines. In the chemical-based tests, H. alpestre extract showed high antioxidant activity, which was maintained even after heat treatment at 121 °C for 30 min. MTT test showed that the sub-cytotoxic concentration of investigated extracts for both microglial cell lines was 40 µg/mL. There were no significant changes in catalase activity during all period of treatment in both cell lines, meanwhile, SOD activity increased (up to 30%) in WT cells during the 48 h treatment. Increase of SOD activity (up to 50%) in Acox^-/- cells was observed under the 24 h treatment. Significant modulation in activity of palmitoyl-CoA oxidase 1 was noticed only during the 48 h treatment of WT microglial cells. These results evidenced the pro-oxidant activity of the investigated extract. This finding can serve as a basis for further evaluation of plant extracts influence on cancer cell lines.

Abbreviations

ACOX1

acyl-CoA oxidase 1

Hypericum alpestre extract

DMEM

Dulbecco's modified Eagle medium

DMSO

dimethyl sulfoxide

DPPH

1,1-diphenyl-2-picrylhydrazyl

EDTA

ethylenediamine tetraacetic acid

essential oil

FBS

fetal bovine serum

MTT

3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide

PBS

Phosphate-buffered saline

RIPA

radioimmunoprecipitation

ROS

reactive oxygen species

SOD

superoxide dismutase

VLCFA

very-long-chain fatty acids

wild type

gallic acid

Acknowledgments

This work was supported by the Science Committee of RA, in the frames of the research project № 21AG-4D027 as well as is based upon work from COST Action NutRedOx-CA16112 supported by COST (European Cooperation in Science and Technology) in the frames of STSM programs. The authors would like to acknowledge Ms. Ashkhen Abrahamyan (Russian-Armenian University) for re-editing the manuscript and improving English.

Conflict of interest

The authors declare no conflict of interest.

Author contributions

All authors contributed to the study's conception and design. NS and MG have carried out the investigations and analyzed the outcomes. NS and MG wrote the manuscript. All authors edited the manuscript. All authors revised and accepted the final version of the manuscript.

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