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Research article Special Issues

Effect of combined training in water on hippocampal neuronal Plasticity and memory function in healthy elderly rats

  • Running title: Effect of Aquatic Training on Hippocampal Plasticity
  • Received: 29 June 2024 Revised: 03 August 2024 Accepted: 15 August 2024 Published: 21 August 2024
  • Purpose 

    The cyclic AMP response element–binding protein (CREB) and nerve growth factor (NGF) have been proposed as key modulators of brain health and are involved in synaptic plasticity. The study investigates how combined water-based training affects hippocampal neuron plasticity and memory function in old rats.

    Methods 

    16 Wistar male rats 24-month-old were randomly divided into two groups: combined training (n = 8) and control (n = 8). Four sessions were performed per week for 10 weeks, and consisted of resistance and endurance training in water. The control group was placed in a water container during training for 30 minutes to be homogenized in terms of the stress conditions. The.NGF and CREB genes in the hippocampus were evaluated and the working memory was measured using real-time PCR and Y-maze tests. The SPSS 26 software was utilized in which independent t-tests were used to analyze the genes and the Mann-Whitney U test was used to analyze functional memory with a significant level of (P < 0.05).

    Results 

    The combined training resulted in a significant rise in NGF and CREB gene expression in the hippocampus tissue of elderly rats compared to the control group (P < 0.05); however, there was no notable difference in the Y maze performance test between the two groups (P < 0.05).

    Conclusions 

    These findings suggest that water-based combined training has beneficial effects on gene expression of NGF and CREB; however, it is necessary to conduct more studies to comprehend the effects of combined training on memory function.

    Citation: Roya. Askari, Mohadeseh. NasrAbadi, Amir Hossein. Haghighi, Mohammad Jahan Mahin, Rajabi Somayeh, Matteo. Pusceddu. Effect of combined training in water on hippocampal neuronal Plasticity and memory function in healthy elderly rats[J]. AIMS Neuroscience, 2024, 11(3): 260-274. doi: 10.3934/Neuroscience.2024017

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  • Purpose 

    The cyclic AMP response element–binding protein (CREB) and nerve growth factor (NGF) have been proposed as key modulators of brain health and are involved in synaptic plasticity. The study investigates how combined water-based training affects hippocampal neuron plasticity and memory function in old rats.

    Methods 

    16 Wistar male rats 24-month-old were randomly divided into two groups: combined training (n = 8) and control (n = 8). Four sessions were performed per week for 10 weeks, and consisted of resistance and endurance training in water. The control group was placed in a water container during training for 30 minutes to be homogenized in terms of the stress conditions. The.NGF and CREB genes in the hippocampus were evaluated and the working memory was measured using real-time PCR and Y-maze tests. The SPSS 26 software was utilized in which independent t-tests were used to analyze the genes and the Mann-Whitney U test was used to analyze functional memory with a significant level of (P < 0.05).

    Results 

    The combined training resulted in a significant rise in NGF and CREB gene expression in the hippocampus tissue of elderly rats compared to the control group (P < 0.05); however, there was no notable difference in the Y maze performance test between the two groups (P < 0.05).

    Conclusions 

    These findings suggest that water-based combined training has beneficial effects on gene expression of NGF and CREB; however, it is necessary to conduct more studies to comprehend the effects of combined training on memory function.



    The exploration of dynamic systems through mathematical models, particularly within the framework of Caputo-Fabrizio fractional-order models, has garnered significant attention in recent years due to its relevance to attractivity, stability, and periodic chaos in discrete dynamical systems. Through a series of research endeavors, including investigations into Caputo-Fabrizio fractional-order fuzzy competitive neural networks (CF-FCNNs, [1]), almost automorphic fuzzy neural networks [2], multi-delay fractional-order differential equations [3], and stochastic inertial neural networks with spatial diffusions [4], researchers have made strides in understanding the dynamics and convergence properties of these systems. These works have contributed new theories, methods, and computational techniques, shedding light on the existence of unique bounded asymptotically almost periodic solutions, global exponential stability, synchronization phenomena, and random periodicity. Collectively, these findings offer valuable insights into the behavior of difference equation systems and their applications across various scientific and engineering domains. Moreover, within the broader realm of mathematical frameworks, rational difference equations and systems emerge as powerful tools for capturing the evolution of discrete dynamical systems. Recently, there has been a noteworthy surge in mathematicians' interest in the intricate dynamics exhibited by rational difference equations and systems of difference equations. This research area has proven exceptionally fruitful, significantly contributing to the foundational theory surrounding the qualitative behavior of nonlinear rational difference equations and systems. Studying rational difference equations and systems is essential for several reasons. First, these equations serve as powerful tools for modeling dynamic systems across various fields, including electrical networks, biology genetics, sociology, probability theory, statistical analyses, stochastic time series, finance, economics, and more. Notably, references such as Ghezal et al. [5,6,7,8] offer insights into asymmetric time series models, while Ghezal et al. [9,10,11] provide valuable contributions to stochastic volatility time series models. Additionally, Ghezal et al. [12,13,14,15,16], as well as works by Elaydi [17], Grove and Ladas [18], Kocic and Ladas [19], and Kulenovic and Ladas [20], serve as exemplary references showcasing the diverse applications of these equations across various fields. By understanding the behavior of rational difference equations and systems, researchers can gain valuable insights into the underlying mechanisms governing complex phenomena observed in real-world systems. Second, rational difference equations and systems offer a versatile framework that can capture nonlinear interactions, delays, and other intricate dynamics present in many practical scenarios. This flexibility allows for the development of mathematical models that accurately represent the complexities of dynamic systems. Moreover, the study of rational difference equations and systems presents both theoretical challenges and practical applications. Analyzing the asymptotic stability and long-term behavior of solutions to rational difference equations and systems can be particularly challenging but is also rewarding in terms of understanding system behavior and making predictions. Additionally, advancements in the theory of rational difference equations and systems contribute to the advancement of mathematical knowledge and its applications. By developing new methods and techniques for analyzing rational difference equations and systems, researchers can expand the boundaries of mathematical theory and enhance our ability to model and predict the behavior of complex systems.

    This paper rigorously explores and analyzes the qualitative behaviors of solutions, placing particular emphasis on both local and global stability within a specific form of a system of rational difference equations, outlined as follows:

    n0,ξn+1=17ψns(7ξn2s1),ψn+1=17ξns(7ψn2s1), (1.1)

    where s is a fixed positive integer. The system is initialized with conditions denoted as ξv, and ψv, v{0,1,,2s+1}, and it is crucial that these initial conditions are real and nonzero.

    Understanding the stability of dynamic systems is not only about unraveling intricate behaviors but also discerning the fundamental elements governing their equilibrium. Interestingly, the solution form of some solvable type difference equations and systems can even be expressed in terms of well-known integer sequences such as Fibonacci numbers, Lucas numbers, generalized Fibonacci numbers, etc. Our aim in this paper is to show that system (1.1) is solvable by deriving its closed-form formulas elegantly and to describe the behavior and periodicity of well-defined solutions to system (1.1). One of the reasons that makes this study interesting is that the solution of system (1.1) is expressed in terms of co-balancing numbers. This investigation delves into the fascinating interplay between global stability and co-balancing numbers within the solutions of rational difference equations. As mathematical structures intimately linked to equilibrium states, co-balancing numbers offer a unique lens through which we can interpret and analyze the stability of the system. The specific number 7 holds significance in our study due to its relevance to the underlying mathematical framework and the properties of the system under investigation. However, we acknowledge that the rationale behind the selection of this particular number is that the solution of system (1.1) is expressed in terms of co-balancing numbers, enabling us to obtain real, non-complex solutions. Numerous researchers have dedicated their efforts to unraveling the qualitative nuances of solutions to rational difference equations, exploring aspects such as global attractivity, boundedness, and periodicity, shedding light on the rich and multifaceted nature of nonlinear mathematical models. For instance, Ghezal [21] investigated the convergence of positive solutions to a rational system of 4(k+1)order difference equations given by:

    n0,ξn+1=ξn(4k+3)1+ψnkψn(2k+1)ψn(3k+2),ψn+1=ψn(4k+3)1+ξnkξn(2k+1)ξn(3k+2),

    where k is a fixed positive integer. El-sayed [22] contributed to the exploration by investigating the behavior of solutions to the nonlinear difference equation:

    n0, ξn+1=αξn1+βξnξn1γξn+δξn1.

    In [23], Ghezal et al. explored the solvability of a bilinear system of difference equations with coefficients dependent on the Jacobsthal sequence. They studied the local stability of the positive solutions. Simșek et al. [24] investigated the solution of the following difference equation

    n0, ξn+1=ξn171+ξn5ξn11.

    In [25], the solution forms for the following nonlinear difference equations were elucidated:

    n0, ξn+1=1±1+ξn.

    Additionally, Zhang et al. [26] conducted an examination on the boundedness and global asymptotic stability of positive solutions within the system of nonlinear difference equations

    n0,ξn+1=α+1ψn+1p,ψn+1=α+ψnξn+1rψn+1s,

    where s is a fixed positive integer. Furthermore, Zhang et al. [27] delved into the dynamics of a system governed by the nonlinear difference equations

    n0,ξn+1=ξn2β+ψnψn1ψn2,ψn+1=ψn2α+ξnξn1ξn2.

    In a demonstration by Okumuș [28], it was shown that the following two-dimensional system of difference equations can be successfully solved:

    n0,ξn+1=±11+ψn(±1+ξn1),ψn+1=±11+ξn(±1ψn1).

    Our paper provides a comprehensive investigation into the local and global stability of a system of rational difference equations, elucidating its connection to co-balancing numbers. This work distinguishes itself through its thorough exploration of the intricate dynamics of mathematical models and their stability properties, with a particular emphasis on the broader implications of global stability. By delving into the role of co-balancing numbers, the paper reveals their significance in achieving equilibrium within the solutions of rational difference equations, thereby enriching our understanding of dynamic systems. In comparison to existing works such as [28] which focus on investigating the solutions, stability characteristics, and asymptotic behavior of rational difference equations associated with Tribonacci numbers, our paper broadens the scope to examine the broader implications of global stability and its connection to co-balancing numbers. This broader perspective offers a more comprehensive understanding of the dynamics and equilibrium properties of dynamic systems. Moreover, while [26,27] concentrate on studying the behavior and stability of specific systems of rational difference equations, our paper extends this analysis to explore the broader concept of global stability and its relationship with co-balancing numbers. Additionally, while [25] and [23] delve into the solutions and local stability of specific types of difference equations associated with Fibonacci and Jacobsthal sequences, respectively, our paper broadens the scope to examine the broader implications of global stability and its connection to co-balancing numbers. This extension enhances our understanding of the stability properties of rational difference equations and offers valuable insights into the factors influencing their equilibrium behavior.

    Recent advancements in computational methods for solving nonlinear evolution equations and partial differential equations (PDEs) have led to the development of innovative techniques such as the bilinear residual network method and the bilinear neural network method. While these methods primarily focus on obtaining exact analytical solutions for nonlinear PDEs, their underlying principles and computational frameworks share similarities with approaches used to solve systems of difference equations. Both systems of difference equations and PDEs describe the evolution of dynamic systems over discrete or continuous domains, respectively, and often exhibit nonlinear behavior. The computational methods proposed in the referenced works [29,30,31,32] leverage neural network architectures and advanced mathematical techniques to tackle the complexities inherent in solving nonlinear equations. Although the specific applications discussed in these references pertain to PDEs, the principles and methodologies underlying these methods can be adapted and extended to address systems of difference equations. By leveraging the flexibility and efficiency of neural networks and tensor-based approaches, researchers can explore new avenues for solving systems of difference equations, advancing our understanding of discrete dynamical systems and their behaviors. Therefore, while the referenced works focus on PDEs, their methodologies and computational frameworks hold promise for addressing challenges in systems of difference equations and related areas of research.

    This paper not only contributes to the academic understanding of mathematical modeling but also provides valuable insights for practitioners in fields where dynamic systems play a crucial role. Through a synthesis of theoretical exploration and illustrative examples, we aim to elucidate the profound implications of global stability and co-balancing numbers in the realm of rational difference equations. For further insights into the system of rational difference equations and related results, researchers can explore the extensive body of work referenced in Abo-Zeid [33], Elsayed et al. [34,35,36], Kara [37], Berkal and Abo-Zeid [38], as well as the contributions by Ghezal and Zemmouri [39,40,41]. These references offer valuable insights into various aspects of rational difference equations, including global behavior, solution structures, periodicity, and stability properties. Additionally, Kara's investigation [37] provides further exploration into the dynamics of exponential-form difference equation systems.

    We begin by recalling co-balancing numbers as defined in [42,43], which will be instrumental in our analysis of the solution to the system of difference equations (1.1).

    Definition 2.1. A positive integer n is termed a balancing number if

    1+2++(n1)=(n+1)+(n+2)++(n+r), (2.1)

    for some rN. Here, r is referred to as the balancer corresponding to the balancing number n. [44] demonstrated in a joint study that the balancing numbers satisfy the following recurrence relation:

    Bn+1=6BnBn1, n1,

    with B0=0 and B1=1. The Binet formula is Bn=(τnκn)/(τκ) for all n0, where κ=322 and τ=3+22.

    Definition 2.2. The quotient of two consecutive terms of balancing numbers tends to a, that is, limBn+1/Bn=τ.

    Definition 2.3. After a slight modification to (2.1), we call a natural number n a co-balancing number if it satisfies:

    1+2++n=(n+1)+(n+2)++(n+r), (2.2)

    and if the pair (n,r) N is a solution to (2.2), then n is called the co-balancing number and r is termed the co-balancer associated with n.

    Definition 2.4. The co-balancing sequence is defined by (bn) for nN, where

    bn+1=6bnbn1+2, n2,

    with b1=0 and b2=2. The Binet formula for bn is given by bn=1/2+(τn1/2κn1/2)/(τκ) for all n0.

    Our exploration of the solutions for system (1.1) involves a nuanced analysis of two distinct cases: one where s=0, and another where s>0.

    When s=0, system (1.1) takes on the following form

    n0,ξn+1=17ψn(7ξn1),ψn+1=17ξn(7ψn1). (3.1)

    In this context, we endeavor to derive closed-form solutions for system (3.1). To achieve this, we utilize the following change of variables, denoted by

    n,ξn=vn1un,ψn=un1vn, (3.2)

    which transforms system (3.1) into its equivalent counterpart:

    n0,{(vnun+1)1=7un1vn(7vn2un1)(unvn+1)1=7vn1un(7un2vn1)n0,un+1=7vn7un1+vn2,vn+1=7un7vn1+un2. (3.3)

    To further simplify the last system, we sum and subtract the two equations and introduce a new variable transformation:

    n, xn+1=un+1+vn+1 and yn+1=un+1vn+1. (3.4)

    Therefore, we arrive at an equivalent system given by: n0,

    xn+1=7xn7xn1+xn2, (3.5)
    yn+1=7yn7yn1yn2. (3.6)

    Through this, we can deduce closed-form expressions as outlined in the following three Lemmas.

    Lemma 3.1. Consider the linear difference equation (3.5) with initial values x2, x1, and x0R. The general solution {xn,n2} is delineated by the following expression:

    n,(κ1)2xn=κ(x2+x0)+(κ2+1)x1+κ2(1κ+1(x2+κx0)x1)Bn+1+(1κκ+1x2+(κτ)x1+τκ2κ+1x0)Bn(1κ+1(κx2+x0)x1)Bn1,

    where (Bn,n1) represents the sequence of balancing numbers.

    Proof. We commence the proof by employing the characteristic polynomial of Eq (3.5):

    λ37λ2+7λ1=(λ1)(λκ)(λτ)=0.

    The roots of this equation are λ1=1, λ2=κ, λ3=τ. Consequently, the closed form of the general solution for difference equation (3.5) is expressed as:

    n2, xn=θ1+θ2κn+θ3τn.

    Here, x2, x1, and x0 denote initial values satisfying:

    x0=θ1+θ2+θ3,x1=θ1+τθ2+κθ3,x2=θ1+τ2θ2+κ2θ3.

    By solving the standard system and conducting subsequent calculations, we obtain:

    (κ1)2θ1=κ(x2+x0)+(κ2+1)x1,(κ1)2θ2=κ2κ+1(x2+κx0)κ2x1,(κ1)2θ3=1κ+1(κx2+x0)x1.

    Hence, we derive the following expression:

    n, (κ1)2xn=κ(x2+x0)+(κ2+1)x1+κ2(1κ+1(x2+κx0)x1)κn+(1κ+1(κx2+x0)x1)τn,

    and after further calculations and simplification, we arrive at:

    n, (κ1)2xn=κ(x2+x0)+(κ2+1)x1+κ2(1κ+1(x2+κx0)x1)Bn+1+(1κκ+1x2+(κτ)x1+τκ2κ+1x0)Bn(1κ+1(κx2+x0)x1)Bn1.

    The lemma is thereby proven.

    Lemma 3.2. Consider the linear difference equation (3.6) with initial values y2, y1, and y0R. The general solution {yn,n2} can be succinctly expressed as:

    n,(κ1)2(1)nyn=κ(y2+y0)(κ2+1)y1+κ2(1κ+1(y2+κy0)+y1)Bn+1+(1κκ+1y2+(τκ)y1+τκ2κ+1y0)Bn(1κ+1(κy2+y0)+y1)Bn1.

    Proof. To establish the solution for the difference equation (3.5), we begin by utilizing its characteristic polynomial:

    λ3+7λ2+7λ+1=(λ+1)(λ+κ)(λ+τ)=0.

    The roots of this polynomial are denoted as λ1=1, λ2=κ, λ3=τ, then the closed form of the general solution for this difference equation is expressed as:

    n2, (1)nyn=ϑ1+ϑ2κn+ϑ3τn.

    Here, y2, y1, and y0 are defined by the standard system:

    y0=ϑ1+ϑ2+ϑ3,y1=ϑ1τϑ2κϑ3,y2=ϑ1+τ2ϑ2+κ2ϑ3.

    Upon solving the standard system and conducting subsequent calculations, the coefficients ϑ1, ϑ2 and ϑ3 are determined as follows:

    (κ1)2ϑ1=κ(y2+y0)(κ2+1)y1.(κ1)2ϑ2=κ2κ+1(y2+κy0)+κ2y1.(κ1)2ϑ3=1κ+1(κy2+y0)+y1.

    Consequently, the final expression for yn is given by:

    n, (κ1)2(1)nyn=κ(y2+y0)(κ2+1)y1+κ2(1κ+1(y2+κy0)+y1)κn+(1κ+1(κy2+y0)+y1)τn.

    Further simplification yields the key result:

    n, (κ1)2(1)nyn=κ(y2+y0)(κ2+1)y1+κ2(1κ+1(y2+κy0)+y1)Bn+1+(1κκ+1y2+(τκ)y1+τκ2κ+1y0)Bn(1κ+1(κy2+y0)+y1)Bn1.

    Thus, the lemma is effectively proven.

    Lemma 3.3. Consider the system of linear difference equations (3.3) with initial values u2, u1, u0, v2, v1, and v0R. The general solution {(un,vn),n2} can be succinctly expressed as:

    n,(κ1)2u2n=κ(u2+u0)+(κ2+1)v1+κ2(1κ+1(u2+κu0)v1)B2n+1+(1κκ+1u2+(κτ)v1+τκ2κ+1u0)B2n(1κ+1(κu2+u0)v1)B2n1,
    n,(κ1)2u2n+1=κ(v2+v0)+(κ2+1)u1+κ2(1κ+1(v2+κv0)u1)B2n+2+(1κκ+1v2+(κτ)u1+τκ2κ+1v0)B2n+1(1κ+1(κv2+v0)u1)B2n,
    n,(κ1)2v2n=κ(v2+v0)+(κ2+1)u1+κ2(1κ+1(v2+κv0)u1)B2n+1+(1κκ+1v2+(κτ)u1+τκ2κ+1v0)B2n(1κ+1(κv2+v0)u1)B2n1,
    n,(κ1)2v2n+1=κ(u2+u0)+(κ2+1)v1+κ2(1κ+1(u2+κu0)v1)B2n+2+(1κκ+1u2+(κτ)v1+τκ2κ+1u0)B2n+1(1κ+1(κu2+u0)v1)B2n.

    Proof. Through the equivalent linear systems (3.5) and (3.6) and by employing the conversely defined change of variables (3.4), un+1 is represented as (xn+1+yn+1)/2 and vn+1  is represented as (xn+1yn+1)/2. The subsequent application of Lemmas 3.1 and 3.2 allows us to deduce the closed-form solution for system (3.3) as:

    n, 2(κ1)2un=κ((x2+(1)ny2)+(x0+(1)ny0))+(κ2+1)(x1+(1)n+1y1)+κ2(1κ+1((x2+(1)ny2)+κ(x0+(1)ny0))(x1(1)ny1))Bn+1+(1κκ+1(x2+(1)ny2)+(κτ)(x1(1)ny1)+τκ2κ+1(x0+(1)ny0))Bn(1κ+1(κ(x2+(1)ny2)+(x0+(1)ny0))(x1+(1)n+1y1))Bn1,
    n, 2(κ1)2vn=κ(((1)ny2x2)+((1)ny0x0))+(κ2+1)(x1+(1)ny1)+κ2(1κ+1(((1)n+1y2+x2)+κ((1)n+1y0+x0))+((1)n+1y1x1))Bn+1+(1κκ+1(x2+(1)n+1y2)+(κτ)(x1(1)n+1y1)+τκ2κ+1(x0+(1)n+1y0))Bn+(1κ+1(κ((1)ny2x2)+((1)ny0x0))+(x1+(1)ny1))Bn1.

    By applying the variable substitution defined in (3.4) once again, the desired results can be obtained. Hence, the lemma is effectively demonstrated.

    Below, we derive a main result specific to this subsection and present it in the form of a theorem.

    Theorem 3.1. Consider the system of nonlinear difference equations (3.1) with initial values ξ1, ξ0, ψ1, and ψ0R. The general solution {(ξn,ψn),n1} can be succinctly expressed as:

    n,ξ2n={κ(ξ0ψ1+1)+(κ2+1)ξ0+κ2(1κ+1(ξ0ψ1+κ)ξ0)B2n+(1κκ+1ξ0ψ1+(κτ)ξ0+τκ2κ+1)B2n1(1κ+1(κξ0ψ1+1)ξ0)B2n2}×{κ(ξ0ψ1+1)+(κ2+1)ξ0+κ2(1κ+1(ξ0ψ1+κ)ξ0)B2n+1+(1κκ+1ξ0ψ1+(κτ)ξ0+τκ2κ+1)B2n(1κ+1(κξ0ψ1+1)ξ0)B2n1}1,
    n,ξ2n+1={κ(ψ0ξ1+1)+(κ2+1)ψ0+κ2(1κ+1(ψ0ξ1+κ)ψ0)B2n+1+(1κκ+1ψ0ξ1+(κτ)ψ0+τκ2κ+1)B2n(1κ+1(κψ0ξ1+1)ψ0)B2n1}×{κ(ψ0ξ1+1)+(κ2+1)ψ0+κ2(1κ+1(ψ0ξ1+κ)ψ0)B2n+2+(1κκ+1ψ0ξ1+(κτ)ψ0+τκ2κ+1)B2n+1(1κ+1(κψ0ξ1+1)ψ0)B2n}1,
    n,ψ2n={κ(ψ0ξ1+1)+(κ2+1)ψ0+κ2(1κ+1(ψ0ξ1+κ)ψ0)B2n+(1κκ+1ψ0ξ1+(κτ)ψ0+τκ2κ+1)B2n1(1κ+1(κψ0ξ1+1)ψ0)B2n2}×{κ(ψ0ξ1+1)+(κ2+1)ψ0+κ2(1κ+1(ψ0ξ1+κ)ψ0)B2n+1+(1κκ+1ψ0ξ1+(κτ)ψ0+τκ2κ+1)B2n(1κ+1(κψ0ξ1+1)ψ0)B2n1)1,
    n,ψ2n+1={κ(ξ0ψ1+1)+(κ2+1)ξ0+κ2(1κ+1(ξ0ψ1+κ)ξ0)B2n+1+(1κκ+1ξ0ψ1+(κτ)ξ0+τκ2κ+1)B2n(1κ+1(κξ0ψ1+1)ξ0)B2n1}×{κ(ξ0ψ1+1)+(κ2+1)ξ0+κ2(1κ+1(ξ0ψ1+κ)ξ0)B2n+2+(1κκ+1ξ0ψ1+(κτ)ξ0+τκ2κ+1)B2n+1(1κ+1(κξ0ψ1+1)ξ0)B2n}1.

    Proof. The proof of this theorem relies on Lemma 3.2 and employs the change of variables (3.2). Consequently, we establish the following relationships denoted as

    n,ξ2n=v2n1u2n,ξ2n+1=v2nu2n+1,ψ2n=u2n1v2n,ψ2n+1=u2nv2n+1,    

    then, for all n,

    n, ξ2n={κ(u2+u0)+(κ2+1)v1+κ2(1κ+1(u2+κu0)v1)B2n+(1κκ+1u2+(κτ)v1+τκ2κ+1u0)B2n1(1κ+1(κu2+u0)v1)B2n2}×{κ(u2+u0)+(κ2+1)v1+κ2(1κ+1(u2+κu0)v1)B2n+1+(1κκ+1u2+(κτ)v1+τκ2κ+1u0)B2n(1κ+1(κu2+u0)v1)B2n1}1={κ(ξ0ψ1+1)+(κ2+1)ξ0+κ2(1κ+1(ξ0ψ1+κ)ξ0)B2n+(1κκ+1ξ0ψ1+(κτ)ξ0+τκ2κ+1)B2n1(1κ+1(κξ0ψ1+1)ξ0)B2n2}×{κ(ξ0ψ1+1)+(κ2+1)ξ0+κ2(1κ+1(ξ0ψ1+κ)ξ0)B2n+1+(1κκ+1ξ0ψ1+(κτ)ξ0+τκ2κ+1)B2n(1κ+1(κξ0ψ1+1)ξ0)B2n1}1.

    The remaining details are straightforward and omitted for brevity.

    Example 3.1. Consider the system (3.1) with initial values ξ1=1.50, ξ0=1.20, ψ1=1.10, and ψ0=1.20. Figure 1 presents a graphical depiction of the sustained dynamics within this system.

    Figure 1.  The behavior of a specific solution of the system (3.1).

    For a 2(s+1)-order system with s>0, our aim is to deduce closed-form solutions for system (1.1). This system is viewed as an extension of system (3.1), represented as follows for all n0:

    ξ(s+1)(n+1)m=17ψ(s+1)nm(7ξ(s+1)(n1)m), ψ(s+1)(n+1)m=17ξ(s+1)nm(7ψ(s+1)(n1)m),

    for m{0,1,,s} and nN. Introducing the notations: Φ(s)n(m)=ξ(s+1)nm, Ψ(s)n(m)=ψ(s+1)nm, for m{0,1,,s} and n0, we can derive (s+1)systems analogous to system (3.1),

    n0, Φ(s)n+1(m)=17Ψ(s)n(m)(7Φ(s)n1(m)), Ψ(s)n+1(m)=17Φ(s)n(m)(7Ψ(s)n1(m)),

    for m{0,1,,s}. Based on this discussion, we present the first main result.

    Theorem 3.2. Consider the system of nonlinear difference equations (1.1) with initial values ξvR and ψvR, v{0,1,,2s+1}. The general solution {(ξn,ψn),n} can be succinctly expressed as: for m{0,1,,s},

    n,ξ(s+1)(2n)m={κ(ξmψ(s+1)m+1)+(κ2+1)ξm+κ2(1κ+1(ξmψ(s+1)m+κ)ξm)B2n+(1κκ+1ξmψ(s+1)m+(κτ)ξm+τκ2κ+1)B2n1(1κ+1(κξmψ(s+1)m+1)ξm)B2n2}×{κ(ξmψ(s+1)m+1)+(κ2+1)ξm+κ2(1κ+1(ξmψ(s+1)m+κ)ξm)B2n+1+(1κκ+1ξmψ(s+1)m+(κτ)ξm+τκ2κ+1)B2n(1κ+1(κξmψ(s+1)m+1)ξm)B2n1}1,
    n,ξ(s+1)(2n+1)m={κ(ψmξ(s+1)m+1)+(κ2+1)ψm+κ2(1κ+1(ψmξ(s+1)m+κ)ψm)B2n+1+(1κκ+1ψmξ(s+1)m+(κτ)ψm+τκ2κ+1)B2n(1κ+1(κψmξ(s+1)m+1)ψm)B2n1}×{κ(ψmξ(s+1)m+1)+(κ2+1)ψm+κ2(1κ+1(ψmξ(s+1)m+κ)ψm)B2n+2+(1κκ+1ψmξ(s+1)m+(κτ)ψm+τκ2κ+1)B2n+1(1κ+1(κψmξ(s+1)m+1)ψm)B2n}1,
    n,ψ(s+1)(2n)m={κ(ψmξ(s+1)m+1)+(κ2+1)ψm+κ2(1κ+1(ψmξ(s+1)m+κ)ψm)B2n+(1κκ+1ψmξ(s+1)m+(κτ)ψm+τκ2κ+1)B2n1(1κ+1(κψmξ(s+1)m+1)ψm)B2n2}×{κ(ψmξ(s+1)m+1)+(κ2+1)ψm+κ2(1κ+1(ψmξ(s+1)m+κ)ψm)B2n+1+(1κκ+1ψmξ(s+1)m+(κτ)ψm+τκ2κ+1)B2n(1κ+1(κψmξ(s+1)m+1)ψm)B2n1},
    n,ψ(s+1)(2n+1)m={κ(ξmψ(s+1)m+1)+(κ2+1)ξm+κ2(1κ+1(ξmψ(s+1)m+κ)ξm)B2n+1+(1κκ+1ξmψ(s+1)m+(κτ)ξm+τκ2κ+1)B2n(1κ+1(κξmψ(s+1)m+1)ξm)B2n1}×{κ(ξmψ(s+1)m+1)+(κ2+1)ξm+κ2(1κ+1(ξmψ(s+1)m+κ)ξm)B2n+2+(1κκ+1ξmψ(s+1)m+(κτ)ξm+τκ2κ+1)B2n+1(1κ+1(κξmψ(s+1)m+1)ξm)B2n}1.

    Proof. Utilizing the (s+1)systems analogous to system (3.1) and applying Theorem 3.1, we conclude the proof of Theorem 3.2.

    In the preceding outcome, the solutions of system (1.1) were represented using a balancing sequence, whereas the next result was articulated using a co-balancing sequence.

    Corollary 3.1. Consider the system of nonlinear difference equations (1.1) with initial values ξvR, and ψvR, v{0,1,,2s+1}. The general solution {(ξn,ψn),n} can be succinctly expressed as: for m{0,1,,s},

    n,ξ(s+1)(2n)m={2κ(ξmψ(s+1)m+1)+2(κ2+1)ξm+κ2(1κ+1(ξmψ(s+1)m+κ)ξm)b2n+1+(1κκ2κ+1ξmψ(s+1)m+(κ2+κτ)ξm+τκ2κ3κ+1)b2n(1κ+1ξmψ(s+1)m+(κτ1)ξm+τκ2+1κ+1)b2n1+(1κ+1(κξmψ(s+1)m+1)ξm)b2n2}×{2κ(ξmψ(s+1)m+1)+2(κ2+1)ξm+κ2(1κ+1(ξmψ(s+1)m+κ)ξm)b2n+2+(1κκ2κ+1ξmψ(s+1)m+(κ2+κτ)ξm+τκ2κ3κ+1)b2n+1(1κ+1ξmψ(s+1)m+(κτ1)ξm+τκ2+1κ+1)b2n+(1κ+1(κξmψ(s+1)m+1)ξm)b2n1}1,
    n,ξ(s+1)(2n+1)m={2κ(ψmξ(s+1)m+1)+2(κ2+1)ψm+κ2(1κ+1(ψmξ(s+1)m+κ)ψm)b2n+2+(1κκ2κ+1ψmξ(s+1)m+(κ2+κτ)ψm+τκ2κ3κ+1)b2n+1(1κ+1ψmξ(s+1)m+(κτ1)ψm+τκ2+1κ+1)b2n+(1κ+1(κψmξ(s+1)m+1)ψm)b2n1}×{2κ(ψmξ(s+1)m+1)+2(κ2+1)ψm+κ2(1κ+1(ψmξ(s+1)m+κ)ψm)b2n+3+(1κκ2κ+1ψmξ(s+1)m+(κ2+κτ)ψm+τκ2κ3κ+1)b2n+2(1κ+1ψmξ(s+1)m+(κτ1)ψm+τκ2+1κ+1)b2n+1+(1κ+1(κψmξ(s+1)m+1)ψm)b2n}1,
    n,ψ(s+1)(2n)m={2κ(ψmξ(s+1)m+1)+2(κ2+1)ψm+κ2(1κ+1(ψmξ(s+1)m+κ)ψm)b2n+1+(1κκ2κ+1ψmξ(s+1)m+(κ2+κτ)ψm+τκ2κ3κ+1)b2n(1κ+1ψmξ(s+1)m+(κτ1)ψm+τκ2+1κ+1)b2n1+(1κ+1(κψmξ(s+1)m+1)ψm)b2n2}×{2κ(ψmξ(s+1)m+1)+2(κ2+1)ψm+κ2(1κ+1(ψmξ(s+1)m+κ)ψm)b2n+2+(1κκ2κ+1ψmξ(s+1)m+(κ2+κτ)ψm+τκ2κ3κ+1)b2n+1(1κ+1ψmξ(s+1)m+(κτ1)ψm+τκ2+1κ+1)b2n+(1κ+1(κψmξ(s+1)m+1)ψm)b2n1}1,
    n,ψ(s+1)(2n+1)m={2κ(ξmψ(s+1)m+1)+2(κ2+1)ξm+κ2(1κ+1(ξmψ(s+1)m+κ)ξm)b2n+2+(1κκ2κ+1ξmψ(s+1)m+(κ2+κτ)ξm+τκ2κ3κ+1)b2n+1(1κ+1ξmψ(s+1)m+(κτ1)ξm+τκ2+1κ+1)b2n+(1κ+1(κξmψ(s+1)m+1)ξm)b2n1}×{2κ(ξmψ(s+1)m+1)+2(κ2+1)ξm+κ2(1κ+1(ξmψ(s+1)m+κ)ξm)b2n+3+(1κκ2κ+1ξmψ(s+1)m+(κ2+κτ)ξm+τκ2κ3κ+1)b2n+2(1κ+1ξmψ(s+1)m+(κτ1)ξm+τκ2+1κ+1)b2n+1+(1κ+1(κξmψ(s+1)m+1)ξm)b2n}1,

    where (bn,n1) represents the sequence of co-balancing numbers.

    Proof. The proof of this corollary establishes the following relation between balancing and co-balancing numbers: 2Bn=bn+1bn. Further details have been omitted.

    Remark 3.1. The general solution to the system of nonlinear difference equations (1.1) with initial values ξvR and ψvR, v{0,1,,2s+1} is of paramount importance. The general solution {(ξn,ψn), n} can be succinctly expressed as follows: for m{0,1,,s}, for every n, ξ(s+1)(2n)m, ξ(s+1)(2n+1)m, ψ(s+1)(2n)m, and ψ(s+1)(2n+1)m can be expressed using the proposed formulas, where (Bn) and (bn) represent balancing and cobalancing numbers. This remark illustrates the method by which solutions to the complex system of difference equations can be accurately expressed, highlighting the importance of employing co-balancing numbers in this context.

    Corollary 3.2. Consider the following nonlinear difference equation

    n0,ξn+1=17ξns(7ξn2s1),

    where s is a fixed positive integer with initial values ξvR, v{0,1,,2s+1}. The general solution {ξn,n} can be succinctly expressed as: for m{0,1,,s},

    n,ξ(s+1)(2n)m={κ(ξmξ(s+1)m+1)+(κ2+1)ξm+κ2(1κ+1(ξmξ(s+1)m+κ)ξm)B2n+(1κκ+1ξmξ(s+1)m+(κτ)ξm+τκ2κ+1)B2n1(1κ+1(κξmξ(s+1)m+1)ξm)B2n2}×{κ(ξmξ(s+1)m+1)+(κ2+1)ξm+κ2(1κ+1(ξmξ(s+1)m+κ)ξm)B2n+1+(1κκ+1ξmξ(s+1)m+(κτ)ξm+τκ2κ+1)B2n(1κ+1(κξmξ(s+1)m+1)ξm)B2n1}1,
    n,ξ(s+1)(2n+1)m={κ(ξmξ(s+1)m+1)+(κ2+1)ξm+κ2(1κ+1(ξmξ(s+1)m+κ)ξm)B2n+1+(1κκ+1ξmξ(s+1)m+(κτ)ξm+τκ2κ+1)B2n(1κ+1(κξmξ(s+1)m+1)ξm)B2n1}×{κ(ξmξ(s+1)m+1)+(κ2+1)ξm+κ2(1κ+1(ξmξ(s+1)m+κ)ξm)B2n+2+(1κκ+1ξmξ(s+1)m+(κτ)ξm+τκ2κ+1)B2n+1(1κ+1(κξmξ(s+1)m+1)ξm)B2n}1.

    Proof. The proof of the general solution for the nonlinear difference equation is derived from Theorem 3.2 when ξv=ψv for v{0,1,,2s+1}.

    Example 3.2. Consider the system (1.1) with s=1 and initial values ξ4=1.50, ξ3=1.20, ξ2=0.30, ξ1=0.40, ξ0=0.50, ψ4=1.10, ψ3=1.20, ψ2=1.30, ψ1=1.40, and ψ0=1.50. Figure 2 presents a graphical depiction of the sustained dynamics within this system.

    Figure 2.  The behavior of a specific solution of the system (1.1) with s=1.

    Example 3.3. Consider the system (1.1) with s=2 and initial values ξv=1v and ψv=1.2exp(v)+2, v{0,1,,6}. Figure 3 presents a graphical depiction of the sustained dynamics within this system.

    Figure 3.  The behavior of a specific solution of the system (1.1) with s=2.

    In this subsection, we first determine the equilibrium points and then proceed to analyze the local and global stability of the solutions of system (1.1). The system has three positive equilibrium points:

    Λ_1=(¯ξ1,¯ψ1)=(1,1), Λ_2=(¯ξ2,¯ψ2)=κ(1,1)and Λ_3=(¯ξ3,¯ψ3)=τ(1,1),

    which are solutions to the standard system:

    ¯ξ=17¯ψ(7¯ξ),¯ψ=17¯ξ(7¯ψ).

    The following theorem provides information on the local stability of the equilibrium point Λ_2 of system (1.1).

    Theorem 3.3. The positive equilibrium point Λ_2 of system (1.1) is locally asymptotically stable.

    Proof. In order to analyze the local stability of system (1.1) around the equilibrium point Λ_2, we employ linearized equations. Let n0, ξ_n+1=Γ(ξ_n), where ξ_n:=(x_n,y_n), x_n=(x(1)n,x(2)n,,x(2s+2)n), y_n=(y(1)n,y(2)n,,y(2s+2)n),

    {x(1)n=ξn,x(2)n=ξn1,,x(2l+2)n=ξn2s1y(1)n=ψn,y(2)n=ψn1,,y(2l+2)n=ψn2s1.

    The mapping Γ is defined as:

    Γ:[0,+)4(s+1)[0,+)4(s+1)Z=(x(1),,x(2s+2),y(1),,y(2s+2))Γ(Z)=(Γ1(Z),x(2),,x(2s+2),Γ2(Z),y(2),,y(2s+2))

    where

    Γ1(Z)=17y(s+1)(7x(2s+2)), Γ2(Z)=17x(s+1)(7y(2s+2)).

    The partial derivatives of Γ1(.) (resp., Γ2(.)) with respect to its components are given by:

    {y(s+1)Γ1(Z)=7x(2s+2)(7y(s+1)(7x(2s+2)))2, x(2s+2)Γ1(Z)=y(s+1)(7y(s+1)(7x(2s+2)))2x(s+1)Γ2(Z)=7y(2s+2)(7x(s+1)(7y(2s+2)))2, y(2s+2)Γ2(Z)=x(s+1)(7x(s+1)(7y(2s+2)))2.

    Given the symbols introduced earlier, we can express the linearized system around the equilibrium point Λ_2 as: Γ(ξ_n)=χsξ_n, where χs is the stability matrix defined as:

    χs=(000000κ(κ27κ+7)21000000010000000000100007κ(κ27κ+7)20000000000000000000000000007κ(κ27κ+7)2000000000000000000000000000000κ(κ27κ+7)2100000001000000000010).

    Upon performing initial computations, we find that the characteristic polynomial of χs is given by:

    Pχs(λ)=|χsλI4(s+1)×4(s+1)|=(λ2s+2)2(7κ)2(κ27κ+7)4λ2s+2κ2(κ27κ+7)4,

    where I4(s+1)×4(s+1) is the identity matrix of size 4(s+1)×4(s+1). By analyzing the characteristic polynomial, we can determine the eigenvalues of χs. After conducting meticulous calculations, we ascertain that the roots of χs satisfy λ2s+2=(174)(70299) or λ2s+2=(17+4)(99702). This implies that all the moduli of every eigenvalue of χs are less than one. Therefore, the positive equilibrium point Λ_2 of system (1.1) is concluded to be locally asymptotically stable.

    Theorem 3.4. Consider the system of nonlinear difference equations (1.1) with initial values ξvR, and ψvR, v{0,1,,2s+1}.

    1) Every positive solution {(ξn,ψn),n} of this system converges to the equilibrium point Λ_2 as n.

    2) The positive equilibrium point Λ_2 of system (1.1) is globally asymptotically stable.

    Proof. (ⅰ) Let {(ξn,ψn), n} be a positive solution of system (1.1). We are interested in calculating the limit of the sequences (ξ(s+1)(2n)m), (ξ(s+1)(2n+1)m), (ψ(s+1)(2n)m), and(ψ(s+1)(2n+1)m), for m{0,1,,s}. To proceed with this calculation, let's start by analyzing the behavior of ξ(s+1)(2n)m as n approaches infinity.

    limnξ(s+1)(2n)m=limn{(κ(ξmψ(s+1)m+1)+(κ2+1)ξm)1B2n+1+κ2(1κ+1(ξmψ(s+1)m+κ)ξm)B2nB2n+1+(1κκ+1ξmψ(s+1)m+(κτ)ξm+τκ2κ+1)B2n1B2n+1(1κ+1(κξmψ(s+1)m+1)ξm)B2n2B2n+1}×{(κ(ξmψ(s+1)m+1)+(κ2+1)ξm)1B2n+1+κ2(1κ+1(ξmψ(s+1)m+κ)ξm)+(1κκ+1ξmψ(s+1)m+(κτ)ξm+τκ2κ+1)B2nB2n+1(1κ+1(κξmψ(s+1)m+1)ξm)B2n1B2n+1}1.

    From the given limits: limnB2nB2n+1=κ, limnB2n1B2n+1=κ2, limnB2n2B2n+1=κ3, and limn1B2n+1=0, we get

    limnξ(s+1)(2n)m={κ3(1κ+1(ξmψ(s+1)m+κ)ξm)+κ2(1κκ+1ξmψ(s+1)m+(κτ)ξm+τκ2κ+1)κ3(1κ+1(κξmψ(s+1)m+1)ξm)}×{κ2(1κ+1(ξmψ(s+1)m+κ)ξm)+κ(1κκ+1ξmψ(s+1)m+(κτ)ξm+τκ2κ+1)κ2(1κ+1(κξmψ(s+1)m+1)ξm)}1=κ,

    for m{0,1,,s}. The same approach can be applied to (ξ(s+1)(2n+1)m), (ψ(s+1)(2n)m), and(ψ(s+1)(2n+1)m), for m{0,1,,s}.

    (ⅱ) The proof of the second assertion follows directly from Theorem 3.3 and the first assertion.

    Remark 3.2. Throughout the examples in this paper, Figures 13 demonstrate that the equilibrium point Λ_2 of system (1.1) is globally asymptotically stable.

    This study provided a comprehensive exploration of the dynamics of a system of rational difference equations, investigating both local and global stability aspects. The findings underscored the intricate relationship between stability properties and co-balancing numbers, revealing their significant impact on achieving equilibrium within the solutions of rational difference equations. By examining the role of co-balancing numbers, we gained insights into the interplay between global stability and co-balancing numbers, enhancing our understanding of dynamic systems' mathematical structures. Moreover, this article serves as a valuable resource for mathematicians, researchers, and scholars interested in the intersection of global stability and co-balancing sequences within rational difference equations. The consistently demonstrated global asymptotic stability of the equilibrium point, supported by examples and figures throughout the paper, underscores the significance of the findings. Further research in this direction holds promise for uncovering additional nuances in the intricate relationship between stability and co-balancing numbers within mathematical modeling.

    In the realm of integrable systems, the quest for analytical solutions to nonlinear PDEs has long been a challenge due to the absence of a universal method. However, recent breakthroughs in symbolic computation, particularly through the application of neural networks proposed by Zhang et al. [45,46,47,48], have opened a new avenue for the analytical treatment of nonlinear PDEs. These innovative approaches pave the way for symbolic computation to tackle the complexities of nonlinear PDEs, laying the groundwork for a universal method for obtaining analytical expressions. While the focus of these works is primarily on obtaining exact solutions for PDEs, the underlying methodologies and principles can inspire new ideas for future work. A potential open problem that arises from this research is the exploration of applying similar computational techniques to systems of difference equations. By adapting the neural network architectures and symbolic computation methods employed in solving nonlinear PDEs, researchers can investigate the feasibility of obtaining analytical solutions for systems of difference equations, thus advancing our understanding of discrete dynamical systems and their behaviors. This interdisciplinary approach holds promise for addressing longstanding challenges in the field and opens avenues for further exploration at the intersection of computational mathematics and dynamical systems theory.

    The authors declare they have not used Artificial Intelligence (AI) tools in the creation of this article.

    The authors extend their appreciation to the Deanship of Scientific Research, Vice Presidency for Graduate Studies and Scientific Research at King Faisal University, Saudi Arabia, for financial support under the annual funding track [5964].

    The authors declare there is no conflicts of interest.


    Acknowledgments



    The authors of this article would like to sincerely thank all those who helped in the implementation of this research.

    Conflict of interest



    The authors declare that there are no conflicts of interest in this research.

    Author contributions



    Conceptualization: Roya Askari
    Data curation: Mohadeseh Nasr Abadi
    Formal analysis: Mohadeseh Nasr Abadi, Roya Askari
    Funding acquisition: ----------
    Methodology: Mohammad Jahan Mahin
    Project administration: Roya Askari- Amir Hossein Haghighi
    Visualization: Roya Askari- Amir Hossein Haghighi, Matteo Pusceddu
    Writing - original draft: Somayeh Rajabi
    Writing - review & editing: Somayeh Rajabi- Roya Askari-Matteo Pusceddu

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