Research article

Establishment of a method to measure the intracellular potassium ion concentration of brain tissue using a simple device

  • Received: 23 May 2024 Revised: 06 August 2024 Accepted: 20 August 2024 Published: 26 August 2024
  • Intracellular potassium ion (K+) concentration is higher than extracellular K+ concentration. Some cells maintain intracellular potassium levels by taking up extracellular potassium. However, investigating these details requires techniques to measure intracellular potassium concentrations. We established a method to easily measure intracellular potassium concentrations using a simpler electrode. The LAQUAtwin K-11 was capable of linearly quantifying potassium concentrations and was not affected by cellular constituents other than nucleic acids; however, it did not tolerate low temperatures. Interference caused by a high concentration of nucleic acids was eliminated by the addition of cations. It was also suggested that the concentration of nucleic acids in the cell suspension was not sufficiently high to interfere with the measurements. Intracellular potassium concentrations increased and decreased in response to extracellular potassium concentrations. Exposure to valinomycin did not decrease the potassium concentration, suggesting that re-uptake of the potassium released outside the cells occurred immediately. Additionally, potassium concentrations could be measured in the brain tissue homogenates using the device. This measurement method can track the relative changes in potassium concentration in cells under various conditions and in tissues of various disease models.

    Citation: Takaya Iwamoto, Minori Fujita, Yukiko Futamata, Teruki Okada, Ryuta Morinaga, Airi Nishi, Toshihiko Kinjo, Koichi Kawada, Kyosuke Uno, Nobuyuki Kuramoto. Establishment of a method to measure the intracellular potassium ion concentration of brain tissue using a simple device[J]. AIMS Neuroscience, 2024, 11(3): 275-288. doi: 10.3934/Neuroscience.2024018

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  • Intracellular potassium ion (K+) concentration is higher than extracellular K+ concentration. Some cells maintain intracellular potassium levels by taking up extracellular potassium. However, investigating these details requires techniques to measure intracellular potassium concentrations. We established a method to easily measure intracellular potassium concentrations using a simpler electrode. The LAQUAtwin K-11 was capable of linearly quantifying potassium concentrations and was not affected by cellular constituents other than nucleic acids; however, it did not tolerate low temperatures. Interference caused by a high concentration of nucleic acids was eliminated by the addition of cations. It was also suggested that the concentration of nucleic acids in the cell suspension was not sufficiently high to interfere with the measurements. Intracellular potassium concentrations increased and decreased in response to extracellular potassium concentrations. Exposure to valinomycin did not decrease the potassium concentration, suggesting that re-uptake of the potassium released outside the cells occurred immediately. Additionally, potassium concentrations could be measured in the brain tissue homogenates using the device. This measurement method can track the relative changes in potassium concentration in cells under various conditions and in tissues of various disease models.



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    Acknowledgments



    This work was supported by the Japan Society for the Promotion of Science (JSPS) Grant-in-Aid for Scientific Research (C) (Grant Numbers: 21K06723 [NK], 21K06681 [TK], and 19K07190 [KU]) of the Ministry of Education, Culture, Sports, Science, and Technology of Japan. We would like to thank Editage (www.editage.jp) for English language editing.

    Conflict of interests



    All authors declare no conflicts of interest in this paper.

    Authors' contribiton



    Conceptualization: NK; Data curation: NK; Formal analysis: TI, HO, RM, AN, NK; Funding acquisition: TK, KU, NK; Investigation: TI, HO, RM, AN; Methodology: TI, HO, RM, AN, NK; Project administration: NK; Resources: NK; Software: NK; Supervision: TK, KK, UK, NK; Validation: KK, UK, NK; Visualization: TI, HO, RM, AN, NK; Roles/Writing - original draft: NK; Writing - review & editing: KK, NK; The full meaning of the initials are as follows: Takaya Iwamoto (TI), Haruki Okada (HO), Ryuta Morinaga (RM), Airi Nishi (AN), Toshihiko Kinjo (TK), Koichi Kawada (KK), Kyosuke Uno (KU), and Nobuyuki Kuramoto (NK).

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