Candida africana and Candida dubliniensis as causes of pediatric candiduria: A study using HWP1 gene size polymorphism

Bahram Nikmanesh; Kazem Ahmadikia; Muhammad Ibrahim Getso; Sanaz Aghaei Gharehbolagh; Shima Aboutalebian; Hossein Mirhendi; Shahram Mahmoudi; Bahram Nikmanesh; Kazem Ahmadikia; Muhammad Ibrahim Getso; Sanaz Aghaei Gharehbolagh; Shima Aboutalebian; Hossein Mirhendi; Shahram Mahmoudi

doi:10.3934/microbiol.2020017

AIMS Microbiology

2020, Volume 6, Issue 3: 272-279. doi: 10.3934/microbiol.2020017

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Research article

Candida africana and Candida dubliniensis as causes of pediatric candiduria: A study using HWP1 gene size polymorphism

1.
Department of Medical Laboratory Sciences, School of Allied Medical Sciences, Tehran University of Medical Sciences, Tehran, Iran
2.
Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
3.
Department of Medical Microbiology and Parasitology, Faculty of Clinical Sciences, College of Health Sciences, Bayero University Kano, PMB 3011, Kano-Nigeria
4.
Department of Medical Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
5.
Department of Medical Parasitology and Mycology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran

Received: 18 June 2020 Accepted: 17 August 2020 Published: 25 August 2020

Background Candida species cause a wide spectrum of disease entities. Candida africana and Candida dubliniensis–members of Candida albicans complex-are currently gaining both clinical and epidemiologic significance.
Materials and methods Totally, 150 pediatric isolates that had previously been identified as C. albicans species complex based on a positive germ tube test were included. The isolates were cultured on CHROMagar Candida medium to ensure their purity and the results of germ tube test. For definitive speciation, PCR amplification and size polymorphism of the hyphal wall protein 1 (HWP1) gene was used. The results of HWP1-PCR were confirmed by sequencing the amplified fragments for randomly selected isolates of C. africana and C. dubliniensis.
Results All 150 isolates included in this study were reconfirmed as C. albicans complex on chromogenic media. Based on the HWP1 gene size polymorphism, 141 (94%) isolates were identified as C. albicans, 2 (1.33%) as C. africana, and 1 (0.67%) as C. dubliniensis. The remaining 6 (4%) C. albicans complex isolates were a mix of C. albicans and C. africana. All isolates of C. africana and C. dubliniensis have been recovered from cases of candiduria.
Conclusion C. africana, either alone or mixed with C. albicans, could be a cause of candiduria among pediatric patients and should not be ignored.
- Candiduria,
- Candida albicans complex,
- Candida africana,
- Candida dubliniensis,
- pediatrics
Citation: Bahram Nikmanesh, Kazem Ahmadikia, Muhammad Ibrahim Getso, Sanaz Aghaei Gharehbolagh, Shima Aboutalebian, Hossein Mirhendi, Shahram Mahmoudi. Candida africana and Candida dubliniensis as causes of pediatric candiduria: A study using HWP1 gene size polymorphism[J]. AIMS Microbiology, 2020, 6(3): 272-279. doi: 10.3934/microbiol.2020017

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Abstract

Background Candida species cause a wide spectrum of disease entities. Candida africana and Candida dubliniensis–members of Candida albicans complex-are currently gaining both clinical and epidemiologic significance.

Materials and methods Totally, 150 pediatric isolates that had previously been identified as C. albicans species complex based on a positive germ tube test were included. The isolates were cultured on CHROMagar Candida medium to ensure their purity and the results of germ tube test. For definitive speciation, PCR amplification and size polymorphism of the hyphal wall protein 1 (HWP1) gene was used. The results of HWP1-PCR were confirmed by sequencing the amplified fragments for randomly selected isolates of C. africana and C. dubliniensis.

Results All 150 isolates included in this study were reconfirmed as C. albicans complex on chromogenic media. Based on the HWP1 gene size polymorphism, 141 (94%) isolates were identified as C. albicans, 2 (1.33%) as C. africana, and 1 (0.67%) as C. dubliniensis. The remaining 6 (4%) C. albicans complex isolates were a mix of C. albicans and C. africana. All isolates of C. africana and C. dubliniensis have been recovered from cases of candiduria.

Conclusion C. africana, either alone or mixed with C. albicans, could be a cause of candiduria among pediatric patients and should not be ignored.

Acknowledgments

This study was financially supported by Tehran University of Medical Sciences, Tehran, Iran (grant No. 96-03-27-37410).

Conflict of interest

All authors declare no conflicts of interest in this paper.

References

[1]	Pfaller MA, Diekema DJ (2007) Epidemiology of invasive candidiasis: a persistent public health problem. Clin Microbiol Rev 20: 133-163. doi: 10.1128/CMR.00029-06
[2]	Charsizadeh A, Mirhendi H, Nikmanesh B, et al. (2018) Microbial epidemiology of candidaemia in neonatal and paediatric intensive care units at the Children's Medical Center, Tehran. Mycoses 61: 22-29. doi: 10.1111/myc.12698
[3]	Odds FC, Bougnoux ME, Shaw DJ, et al. (2007) Molecular phylogenetics of Candida albicans. Eukaryotic cell 6: 1041-1052. doi: 10.1128/EC.00041-07
[4]	Romeo O, Criseo G (2008) First molecular method for discriminating between Candida africana, Candida albicans, and Candida dubliniensis by using hwp1 gene. Diagn Microbiol Infect Dis 62: 230-233. doi: 10.1016/j.diagmicrobio.2008.05.014
[5]	Zhu Y, Shi Y, Fan S, et al. (2019) Multilocus sequence typing analysis of Candida africana from vulvovaginal candidiasis. BMC Infect Dis 19: 461. doi: 10.1186/s12879-019-4071-7
[6]	Scordino F, Giuffrè L, Felice MR, et al. (2019) Genetic diversity of Candida albicans isolates recovered from hospital environments and patients with severe acquired brain injuries. Infect Genet Evol 76: 104068. doi: 10.1016/j.meegid.2019.104068
[7]	Sardi J, Scorzoni L, Bernardi T, et al. (2013) Candida species: current epidemiology, pathogenicity, biofilm formation, natural antifungal products and new therapeutic options. J Med Microbiol 62: 10-24. doi: 10.1099/jmm.0.045054-0
[8]	Das KH, Getso MI, Azeez-Ak, et al. (2016) Distribution of Candida albicans and non-albicans Candida in clinical samples and their intrinsic biofilm production status. Int J Med Sci Public Health 5: 2443-2447. doi: 10.5455/ijmsph.2016.26042016491
[9]	Hana S, Latifa M, Camilia C, et al. (2020) Characterization of the ‘Candida albicans Complex’: First Report of Candida africana in Tunisia. J Med Microb Diagn 9: 307.
[10]	Farahyar S, Izadi S, Razmjou E, et al. (2020) Low prevalence of antifungal resistant Candida africana, in the C. albicans complex causing vulvovaginal candidiasis. Heliyon 6: e03619. doi: 10.1016/j.heliyon.2020.e03619
[11]	Mucci MJ, Cuestas ML, Landanburu MF, et al. (2017) Prevalence of Candida albicans, Candida dubliniensis and Candida africana in pregnant women suffering from vulvovaginal candidiasis in Argentina. Rev Iberoam Micol 34: 72-76. doi: 10.1016/j.riam.2016.09.001
[12]	Aghaei Gharehbolagh S, Fallah B, Izadi A, et al. (2020) Distribution, antifungal susceptibility pattern and intra-Candida albicans species complex prevalence of Candida africana: A systematic review and meta-analysis. PLoS ONE 15: e0237046. doi: 10.1371/journal.pone.0237046
[13]	Sharifynia S, Badali H, Sharifi Sorkherizi M, et al. (2016) In vitro antifungal susceptibility profiles of Candida albicans complex isolated from patients with respiratory infections. Acta Med Iran 54: 376-381.
[14]	Mokaddas E, Khan ZU, Ahmad S (2011) Prevalence of Candida dubliniensis among cancer patients in Kuwait: a 5-year retrospective study. Mycoses 54: e29-e34. doi: 10.1111/j.1439-0507.2009.01822.x
[15]	Loreto ÉS, Scheid LA, Nogueira CW, et al. (2010) Candida dubliniensis: epidemiology and phenotypic methods for identification. Mycopathologia 169: 431-443. doi: 10.1007/s11046-010-9286-5
[16]	Ngouana TK, Krasteva D, Drakulovski P, et al. (2015) Investigation of minor species Candida africana, Candida stellatoidea and Candida dubliniensis in the Candida albicans complex among Yaoundé (Cameroon) HIV-infected patients. Mycoses 58: 33-39. doi: 10.1111/myc.12266
[17]	Ciardo D, Schär G, Böttger E, et al. (2006) Internal transcribed spacer sequencing versus biochemical profiling for identification of medically important yeasts. J Clin Microbiol 44: 77-84. doi: 10.1128/JCM.44.1.77-84.2006
[18]	Romeo O, Criseo G (2011) Candida africana and its closest relatives. Mycoses 54: 475-486. doi: 10.1111/j.1439-0507.2010.01939.x
[19]	Theill L, Dudiuk C, Morano S, et al. (2016) Prevalence and antifungal susceptibility of Candida albicans and its related species Candida dubliniensis and Candida africana isolated from vulvovaginal samples in a hospital of Argentina. Rev Argent Microbiol 48: 43-49.
[20]	Rezazadeh E, Moazeni M, Sabokbar A (2016) Use of cost effective and rapid molecular tools for identification of Candida species, opportunistic pathogens. Curr Med Mycol 2: 1-4. doi: 10.18869/acadpub.cmm.2.3.1
[21]	Gajdács M, Dóczi I, Ábrók M, et al. (2019) Epidemiology of candiduria and Candida urinary tract infections in inpatients and outpatients: Results from a 10-year retrospective survey. Cent European J Urol 72: 209-214.
[22]	Hsu JF, Lai MY, Lee CW, et al. (2018) Comparison of the incidence, clinical features and outcomes of invasive candidiasis in children and neonates. BMC Infect Dis 18: 194. doi: 10.1186/s12879-018-3100-2
[23]	Noni M, Stathi A, Vaki I, et al. (2019) Changing epidemiology of invasive candidiasis in children during a 10-year period. J Fungi (Basel) 5: 19. doi: 10.3390/jof5010019
[24]	Haddadi P, Zareifar S, Badiee P, et al. (2014) Yeast colonization and drug susceptibility pattern in the pediatric patients with neutropenia. Jundishapur J Microbiol 7: e11858.
[25]	Mohammadi R, Ataei B (2016) Candidiasis in pediatrics; identification and in vitro antifungal susceptibility of the clinical isolates. Iran J Ped Hematol Oncol 6: 43-51.
[26]	Romeo O, Criseo G (2009) Molecular epidemiology of Candida albicans and its closely related yeasts Candida dubliniensis and Candida africana. J Clin Microbiol 47: 212-214. doi: 10.1128/JCM.01540-08
[27]	Solimani P, Salari S, Hassanzad M, et al. (2014) Use of PCR-RFLP and PCR-HWP1 for identification of Candida species isolated from cystic fibrosis patients. Res Mol Med 2: 23-27. doi: 10.18869/acadpub.rmm.2.3.23
[28]	Yazdanparast SA, Khodavaisy S, Fakhim H, et al. (2015) Molecular characterization of highly susceptible Candidaafricana from vulvovaginal candidiasis. Mycopathologia 180: 317-323. doi: 10.1007/s11046-015-9924-z
[29]	Gumral R, Sancak B, Guzel AB, et al. (2011) Lack of Candida africana and Candida dubliniensis in vaginal Candida albicans isolates in Turkey using HWP1 gene polymorphisms. Mycopathologia 172: 73-76. doi: 10.1007/s11046-011-9401-2

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