Hydrogen production by newly isolated <em>Clostridium</em> species from cow rumen in pure- and co-cultures on a broad range of carbon sources

Vui Thi Hoang; Diem Huong Hoang; Ngoc Duc Pham; Hanh My Tran; Ha Thi Viet Bui; Tien Anh Ngo; Vui Thi Hoang; Diem Huong Hoang; Ngoc Duc Pham; Hanh My Tran; Ha Thi Viet Bui; Tien Anh Ngo

doi:10.3934/energy.2018.5.846

AIMS Energy

2018, Volume 6, Issue 5: 846-865. doi: 10.3934/energy.2018.5.846

Previous Article Next Article

Research article

Hydrogen production by newly isolated Clostridium species from cow rumen in pure- and co-cultures on a broad range of carbon sources

1.
Department of Microbiology, Hanoi University of Science, Vietnam National University, Hanoi, Vietnam
2.
Current address: Laboratory of Applied Micro and Nanotechnology (LAMINATE), Division of Microbiology and Production, National Food Institute, Technical University of Denmark. Kemitorvet, Building 204, DK 2800 Lyngby Denmark

Received: 11 August 2018 Accepted: 11 October 2018 Published: 18 October 2018

Three novel hydrogen-generating strains, ST1, ST4, and ST5, were isolated from the rumen of cow in Vietnam, and respectively identified as Clostridium beijerinckii ST1, Clostridium bifermentans ST4, and Clostridium butyricum ST5, based on 16S rDNA gene sequence analysis and physiobiochemical characteristics. The dark fermentative hydrogen production of these isolated Clostridium strains was performed and characterized in both pure- and co-cultures from various carbon sources including sucrose, glucose, lactose, xylose, molasses, cassava stumps, and rice distillers wet grains with soluble. The highest hydrogen production was achieved from a co-culture with three Clostridium strains. To optimize the operational conditions of temperature, time, and substrate concentration for the high-level production of hydrogen, response surface methodology in a Box-Behnken design was used. The results revealed a maximum hydrogen production of 1.13 ± 0.015 L H₂/L medium by the three-strain co-culture under the following fermentation conditions: 11.63 g/L sucrose, 36.1 °C, in 51.13 h.
- hydrogen production,
- Clostridium,
- co-culture,
- cow rumen bacteria,
- response surface methodology,
- food industry wastes
Citation: Vui Thi Hoang, Diem Huong Hoang, Ngoc Duc Pham, Hanh My Tran, Ha Thi Viet Bui, Tien Anh Ngo. Hydrogen production by newly isolated Clostridium species from cow rumen in pure- and co-cultures on a broad range of carbon sources[J]. AIMS Energy, 2018, 6(5): 846-865. doi: 10.3934/energy.2018.5.846

Related Papers:

Abstract

Three novel hydrogen-generating strains, ST1, ST4, and ST5, were isolated from the rumen of cow in Vietnam, and respectively identified as Clostridium beijerinckii ST1, Clostridium bifermentans ST4, and Clostridium butyricum ST5, based on 16S rDNA gene sequence analysis and physiobiochemical characteristics. The dark fermentative hydrogen production of these isolated Clostridium strains was performed and characterized in both pure- and co-cultures from various carbon sources including sucrose, glucose, lactose, xylose, molasses, cassava stumps, and rice distillers wet grains with soluble. The highest hydrogen production was achieved from a co-culture with three Clostridium strains. To optimize the operational conditions of temperature, time, and substrate concentration for the high-level production of hydrogen, response surface methodology in a Box-Behnken design was used. The results revealed a maximum hydrogen production of 1.13 ± 0.015 L H₂/L medium by the three-strain co-culture under the following fermentation conditions: 11.63 g/L sucrose, 36.1 °C, in 51.13 h.

References

[1]	Krishna RH (2013) Review of research on production methods of hydrogen : future fuel. Eur J Biotechnol Biosci 1: 84–93.
[2]	Das D, Veziroglu TN (2008) Advances in biological hydrogen production processes. Int J Hydrogen Energy 33: 6046–6057. doi: 10.1016/j.ijhydene.2008.07.098
[3]	Gupta SK, Kumari S, Reddy K, et al. (2013) Trends in biohydrogen production: major challenges and state-of-the-art developments. Environ Technol 34: 1653–1670. doi: 10.1080/09593330.2013.822022
[4]	Ntaikou I, Antonopoulou G, Lyberatos G (2010) Biohydrogen production from biomass and wastes via dark fermentation: A review. Waste Biomass Valori 1: 21–39 . doi: 10.1007/s12649-009-9001-2
[5]	Rittmann S, Herwig C (2012) A comprehensive and quantitative review of dark fermentative biohydrogen production. Microb Cell Fact 11: 115. doi: 10.1186/1475-2859-11-115
[6]	Peters JW, Schut GJ, Boyd ES, et al. (2015) [FeFe]- and [NiFe]-hydrogenase diversity, mechanism, and maturation. BBA-Mol Cell Res 1853: 1350–1369.
[7]	Hallenbeck PC (2009) Fermentative hydrogen production: Principles, progress, and prognosis. Int J Hydrogen Energy 34: 7379–7389. doi: 10.1016/j.ijhydene.2008.12.080
[8]	Lee KS, Hsu YF, Lo YC, et al. (2008) Exploring optimal environmental factors for fermentative hydrogen production from starch using mixed anaerobic microflora. Int J Hydrogen Energy 33: 1565–1572. doi: 10.1016/j.ijhydene.2007.10.019
[9]	Masset J, Calusinska M, Hamilton C, et al. (2012) Fermentative hydrogen production from glucose and starch using pure strains and artificial co-cultures of Clostridium spp. Biotechnol Biofuels 5: 35. doi: 10.1186/1754-6834-5-35
[10]	Pan CM, Fan YT, Zhao P, et al. (2008) Fermentative hydrogen production by the newly isolated Clostridium beijerinckii Fanp3. Int J Hydrogen Energy 33: 5383–5391. doi: 10.1016/j.ijhydene.2008.05.037
[11]	Wang H, Ma S, Bu H (2014) Fermentative hydrogen production by newly isolated Clostridium perfringens ATCC 13124. J Renew Sustain Energy 6: 013130. doi: 10.1063/1.4863085
[12]	Zhao X, Xing D, Fu N, et al. (2011) Hydrogen production by the newly isolated Clostridium beijerinckii RZF-1108. Bioresour Technol 102: 8432–8436.
[13]	Zhang H, Bruns MA, Logan BE (2006) Biological hydrogen production by Clostridium acetobutylicum in an unsaturated flow reactor. Water Res 40: 728–734. doi: 10.1016/j.watres.2005.11.041
[14]	Wong YM, Wu TY, Ling TC, et al. (2018) Evaluating new bio-hydrogen producers: Clostridium perfringens strain JJC, Clostridium bifermentans strain WYM and Clostridium sp. strain Ade.TY. J Biosci Bioeng 125: 590–598. doi: 10.1016/j.jbiosc.2017.12.012
[15]	Wong YM, Juan JC, Ting A, et al. (2014) High efficiency bio-hydrogen production from glucose revealed in an inoculum of heat-pretreated landfill leachate sludge. Energy 72: 628–635. doi: 10.1016/j.energy.2014.05.088
[16]	Puniya AK, Singh R, Kamra DN (2015) Rumen Microbiology: From Evolution to Revolution, New Delhi: Springer, 1–379.
[17]	Krause DO, Denman SE, Mackie RI, et al. (2003) Opportunities to improve fiber degradation in the rumen: Microbiology, ecology, and genomics. Fems Microbiol Rev 27: 663–693. doi: 10.1016/S0168-6445(03)00072-X
[18]	Nissilä ME, Tähti HP, Rintala JA, et al. (2011) Thermophilic hydrogen production from cellulose with rumen fluid enrichment cultures: Effects of different heat treatments. Int J Hydrogen Energy 36: 1482–1490. doi: 10.1016/j.ijhydene.2010.11.010
[19]	Ratti RP, Botta LS, Sakamoto IK, et al. (2014) Production of H₂ from cellulose by rumen microorganisms: Effects of inocula pre-treatment and enzymatic hydrolysis. Biotechnol Lett 36: 537–546. doi: 10.1007/s10529-013-1395-z
[20]	Vos P, Garrity G, Jones D, et al. (2009) Bergey's Manual of Systematic Bacteriology, Vol 3: The Firmicutes, New York: Springer-Verlag.
[21]	Miller GL (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem 31: 426–428. doi: 10.1021/ac60147a030
[22]	Lin PY, Whang LM, Wu YR, et al. (2007) Biological hydrogen production of the genus Clostridium: Metabolic study and mathematical model simulation. Int J Hydrogen Energy 32: 1728–1735. doi: 10.1016/j.ijhydene.2006.12.009
[23]	Chong ML, Sabaratnam V, Shirai Y, et al. (2009) Biohydrogen production from biomass and industrial wastes by dark fermentation. Int J Hydrogen Energy 34: 3277–3287. doi: 10.1016/j.ijhydene.2009.02.010
[24]	Karube I, Urano N, Matsunaga T, et al. (1982) Hydrogen production from glucose by imoobilized growing cells of Clostridium butyricum. Eur J Appl Microbiol Biotechnol 16: 5–9. doi: 10.1007/BF01008235
[25]	Karube I, Matsunaga T, Tsuru S, et al. (1976) Continuous hydrogen production by immobilized whole cells of Clostridium Butyricum. BBA-Gen Subj 444: 338–343. doi: 10.1016/0304-4165(76)90376-7
[26]	Beckers L, Hiligsmann S, Hamilton C, et al. (2010) Fermentative hydrogen production by Clostridium butyricum CWBI1009 and Citrobacter freundii CWBI952 in pure and mixed cultures. Biotechnol Agron Soc Environ 14: 541–548.
[27]	Beckers L, Hiligsmann S, Lambert SD, et al. (2013) Improving effect of metal and oxide nanoparticles encapsulated in porous silica on fermentative biohydrogen production by Clostridium butyricum. Bioresour Technol 133: 109–117. doi: 10.1016/j.biortech.2012.12.168
[28]	Beckers L, Masset J, Hamilton C, et al. (2015) Investigation of the links between mass transfer conditions, dissolved hydrogen concentration and biohydrogen production by the pure strain Clostridium butyricum CWBI1009. Biochem Eng J 98: 18–28. doi: 10.1016/j.bej.2015.01.008
[29]	Calusinska M, Hamilton C, Monsieurs P, et al. (2015) Genome-wide transcriptional analysis suggests hydrogenase- and nitrogenase-mediated hydrogen production in Clostridium butyricum CWBI 1009. Biotechnol Biofuels 8: 27. doi: 10.1186/s13068-015-0203-5
[30]	Hiligsmann S, Beckers L, Masset J, et al. (2014) Improvement of fermentative biohydrogen production by Clostridium butyricum CWBI1009 in sequenced-batch, horizontal fixed bed and biodisc-like anaerobic reactors with biomass retention. Int J Hydrogen Energy 39: 6899–6911. doi: 10.1016/j.ijhydene.2014.02.139
[31]	Masset J, Hiligsmann S, Hamilton C, et al. (2010) Effect of pH on glucose and starch fermentation in batch and sequenced-batch mode with a recently isolated strain of hydrogen-producing Clostridium butyricum CWBI1009. Int J Hydrogen Energy 35: 3371–3378. doi: 10.1016/j.ijhydene.2010.01.061
[32]	Jenol MA, Ibrahim MF, Yee PL, et al. (2014) Sago biomass as a sustainable source for biohydrogen production by Clostridium butyricum A1. Bioresources 9: 1007–1026.
[33]	Seelert T, Ghosh D, Yargeau V (2015) Improving biohydrogen production using Clostridium beijerinckii immobilized with magnetite nanoparticles. Appl Microbiol Biotechnol 99: 4107–4116. doi: 10.1007/s00253-015-6484-6
[34]	Wang X, Hoefel D, Saint CP, et al. (2007) The isolation and microbial community analysis of hydrogen producing bacteria from activated sludge. J Appl Microbiol 103: 1415–1423. doi: 10.1111/j.1365-2672.2007.03370.x
[35]	Koskinen PEP, Beck SR, Örlygsson J, et al. (2008) Ethanol and hydrogen production by two thermophilic, anaerobic bacteria isolated from Icelandic geothermal areas. Biotechnol Bioeng 101: 679–690. doi: 10.1002/bit.21942
[36]	Mitchell RJ, Kim JS, Jeon BS, et al. (2009) Continuous hydrogen and butyric acid fermentation by immobilized Clostridium tyrobutyricum ATCC 25755: Effects of the glucose concentration and hydraulic retention time. Bioresour Technol 100: 5352–5355. doi: 10.1016/j.biortech.2009.05.046
[37]	Chen WM, Tseng ZJ, Lee KS, et al. (2005) Fermentative hydrogen production with CGS5 isolated from anaerobic sewage sludge. Int J Hydrogen Energy 30: 1063–1070. doi: 10.1016/j.ijhydene.2004.09.008
[38]	Takara K, Ushijima K, Wada K, et al. (2007) Phenolic compounds from sugarcane molasses possessing antibacterial activity against cariogenic bacteria. J Oleo Sci 56: 611–614. doi: 10.5650/jos.56.611
[39]	Ding J, Liu BF, Ren NQ, et al. (2009) Hydrogen production from glucose by co-culture of Clostridium Butyricum and immobilized Rhodopseudomonas faecalis RLD-53. Int J Hydrogen Energy 34: 3647–3652.
[40]	Geng A, He Y, Qian C, et al. (2010) Effect of key factors on hydrogen production from cellulose in a co-culture of Clostridium thermocellum and Clostridium thermopalmarium. Bioresour Technol 101: 4029–4033. doi: 10.1016/j.biortech.2010.01.042
[41]	Liu Y, Yu P, Song X, et al. (2008) Hydrogen production from cellulose by co-culture of Clostridium thermocellum JN4 and Thermoanaerobacterium thermosaccharolyticum GD17. Int J Hydrogen Energy 33: 2927–2933. doi: 10.1016/j.ijhydene.2008.04.004
[42]	Chang JJ, Chou CH, Ho CY, et al. (2008) Syntrophic co-culture of aerobic Bacillus and anaerobic Clostridium for bio-fuels and bio-hydrogen production. Int J Hydrogen Energy 33: 5137–5146. doi: 10.1016/j.ijhydene.2008.05.021
[43]	Reid SJ, Abratt VR (2005) Sucrose utilisation in bacteria: Genetic organisation and regulation. Appl Microbiol Biotechnol 67: 312–321. doi: 10.1007/s00253-004-1885-y
[44]	Chen SD, Lee KS, Lo YC, et al. (2008) Batch and continuous biohydrogen production from starch hydrolysate by Clostridium species. Int J Hydrogen Energy 33: 1803–1812. doi: 10.1016/j.ijhydene.2008.01.028
[45]	O-Thong S, Hniman A, Prasertsan P, et al. (2011) Biohydrogen production from cassava starch processing wastewater by thermophilic mixed cultures. Int J Hydrogen Energy 36: 3409–3416.
[46]	Su H, Cheng J, Zhou J, et al. (2009) Improving hydrogen production from cassava starch by combination of dark and photo fermentation. Int J Hydrogen Energy 34: 1780–1786. doi: 10.1016/j.ijhydene.2008.12.045
[47]	Wang J, Wan W (2009) Factors influencing fermentative hydrogen production: A review. Int J Hydrogen Energy 34: 799–811. doi: 10.1016/j.ijhydene.2008.11.015
[48]	Wang X, Monis PT, Saint CP, et al. (2009) Biochemical kinetics of fermentative hydrogen production by Clostridium butyricum W5. Int J Hydrogen Energy 34: 791–798. doi: 10.1016/j.ijhydene.2008.11.023
[49]	An D, Li Q, Wang X, et al. (2014) Characterization on hydrogen production performance of a newly isolated Clostridium beijerinckii YA001 using xylose. Int J Hydrogen Energy 39: 19928–19936. doi: 10.1016/j.ijhydene.2014.10.014
[50]	Yin Y, Wang J (2017) Isolation and characterization of a novel strain Clostridium butyricum INET1 for fermentative hydrogen production. Int J Hydrogen Energy 42: 12173–12180. doi: 10.1016/j.ijhydene.2017.02.083
[51]	Junghare M, Subudhi S, Lal B (2012) Improvement of hydrogen production under decreased partial pressure by newly isolated alkaline tolerant anaerobe, Clostridium butyricum TM-9A: Optimization of process parameters. Int J Hydrogen Energy 37: 3160–3168. doi: 10.1016/j.ijhydene.2011.11.043
[52]	Chong ML, Rahim RA, Shirai Y, et al. (2009) Biohydrogen production by Clostridium butyricum EB6 from palm oil mill effluent. Int J Hydrogen Energy 34: 764–771. doi: 10.1016/j.ijhydene.2008.10.095
[53]	Yu HQ, Fang HH (2001) Acidification of mid- and high-strength dairy wastewaters. Water Res 35: 3697–3705. doi: 10.1016/S0043-1354(01)00077-X
[54]	Lo YC, Chen WM, Hung CH, et al. (2008) Dark H₂ fermentation from sucrose and xylose using H₂-producing indigenous bacteria: Feasibility and kinetic studies. Water Res 42: 827–842. doi: 10.1016/j.watres.2007.08.023
[55]	Ginkel SV, Sung S, Lay JJ (2001) Biohydrogen production as a function of pH and substrate concentration. Environ Sci Technol 35: 4726–4730. doi: 10.1021/es001979r
[56]	Hawkes FR, Dinsdale R, Hawkes DL, et al. (2002) Sustainable fermentative hydrogen production: Challenges for process optimisation. Int J Hydrogen Energy 27: 1339–1347. doi: 10.1016/S0360-3199(02)00090-3
[57]	Lay JJ (2001) Biohydrogen generation by mesophilic anaerobic fermentation of microcrystalline cellulose. Biotechnol Bioeng 74: 280–287. doi: 10.1002/bit.1118
[58]	Lin CY, Chang CC, Hung CH (2008) Fermentative hydrogen production from starch using natural mixed cultures. Int J Hydrogen Energy 33: 2445–2453.
[59]	Chen WH, Chen SY, Khanal SK, et al. (2006) Kinetic study of biological hydrogen production by anaerobic fermentation. Int J Hydrogen Energy 31: 2170–2178.
[60]	Park JH, Kim DH, Kim SH, et al. (2018) Effect of substrate concentration on the competition between Clostridium and Lactobacillus during biohydrogen production. Int J Hydrogen Energy 43: 11460–11469. doi: 10.1016/j.ijhydene.2017.08.150
[61]	Guo WQ, Ren NQ, Wang XJ, et al. (2009) Optimization of culture conditions for hydrogen production by Ethanoligenens harbinense B49 using response surface methodology. Bioresour Technol 100: 1192–1196. doi: 10.1016/j.biortech.2008.07.070

Reader Comments

Your name:*

Email:*
© 2018 the Author(s), licensee AIMS Press. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0)