Identification of dysregulated pathways through <i>SLC30A8</i> protein interaction in type 1 diabetes mellitus

Afreen Bhatty; Zile Rubab; Hafiz Syed Mohammad Osama Jafri; Sheh Zano; Afreen Bhatty; Zile Rubab; Hafiz Syed Mohammad Osama Jafri; Sheh Zano

doi:10.3934/molsci.2021023

AIMS Molecular Science

2021, Volume 8, Issue 4: 301-310. doi: 10.3934/molsci.2021023

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Research article

Identification of dysregulated pathways through SLC30A8 protein interaction in type 1 diabetes mellitus

Department of Biochemistry, Ziauddin University, Karachi, Pakistan

Received: 29 October 2021 Accepted: 20 December 2021 Published: 27 December 2021

Objective
The aim of the current study was to explore the gene enrichment and dysregulated pathways on the basis of interaction network analysis of SLC30A8 in type 1 diabetes mellitus (T1DM). SLC30A8 polymorphism could be characterized as a beneficial tool to identify the interacting gene in developing T1DM.

Materials and methods
SLC30A8 interacting protein interaction network was obtained by String Interaction network Version 11.0. Ten proteins were identified interacting with SLC30A8 and were analysed by protein-protein interaction and enrichment network analysis along with Functional Enrichment analysis tool (FunRich 3.1.3) to map the gene data sets. In entire analysis, FunRich database was used as background against all annotated gene/protein list. Protein-protein interaction (PPI) and enrichment network analysis of the selected protein: SLC30A8 gene along with gene mapping and pathway enrichment were performed using FunRich 3.1.3 and String Interaction network Version 11.0.

Results
Biological pathway grouping displayed enriched proteins in TRAIL signalling pathway (p < 0.001). PTPRN, GAD2 and TCF7L2 were enriched in TRAIL Signalling pathway when INS was made focused gene and directly interacting with SLC30A8.

Conclusions
TRAIL signalling pathways were enriched in T1DM. Therefore, SLC30A8 along with PTPRN, GAD2 and TCF7L2 involved in TRAIL pathway must be further explored to understand their in vivo role in T1DM.
- TRAIL,
- SLC30A8,
- diabetes mellitus,
- type 1,
- pathways,
- protein interaction
Citation: Afreen Bhatty, Zile Rubab, Hafiz Syed Mohammad Osama Jafri, Sheh Zano. Identification of dysregulated pathways through SLC30A8 protein interaction in type 1 diabetes mellitus[J]. AIMS Molecular Science, 2021, 8(4): 301-310. doi: 10.3934/molsci.2021023

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Abstract

Objective

The aim of the current study was to explore the gene enrichment and dysregulated pathways on the basis of interaction network analysis of SLC30A8 in type 1 diabetes mellitus (T1DM). SLC30A8 polymorphism could be characterized as a beneficial tool to identify the interacting gene in developing T1DM.

Materials and methods

SLC30A8 interacting protein interaction network was obtained by String Interaction network Version 11.0. Ten proteins were identified interacting with SLC30A8 and were analysed by protein-protein interaction and enrichment network analysis along with Functional Enrichment analysis tool (FunRich 3.1.3) to map the gene data sets. In entire analysis, FunRich database was used as background against all annotated gene/protein list. Protein-protein interaction (PPI) and enrichment network analysis of the selected protein: SLC30A8 gene along with gene mapping and pathway enrichment were performed using FunRich 3.1.3 and String Interaction network Version 11.0.

Results

Biological pathway grouping displayed enriched proteins in TRAIL signalling pathway (p < 0.001). PTPRN, GAD2 and TCF7L2 were enriched in TRAIL Signalling pathway when INS was made focused gene and directly interacting with SLC30A8.

Conclusions

TRAIL signalling pathways were enriched in T1DM. Therefore, SLC30A8 along with PTPRN, GAD2 and TCF7L2 involved in TRAIL pathway must be further explored to understand their in vivo role in T1DM.

Conflict of interest

The authors declare no conflict of interest.

References

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