Cranberry (<i>Vaccinium macrocarpon</i>) dietary supplementation and fecal microbiota of Wistar rats

Rayane Chettaoui; Gilles Mayot; Loris De Almeida; Patrick Di Martino; Rayane Chettaoui; Gilles Mayot; Loris De Almeida; Patrick Di Martino

doi:10.3934/microbiol.2021016

AIMS Microbiology

2021, Volume 7, Issue 2: 257-270. doi: 10.3934/microbiol.2021016

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Cranberry (Vaccinium macrocarpon) dietary supplementation and fecal microbiota of Wistar rats

Laboratoire ERRMECe, CY Cergy Paris University, 1 rue Descartes 95000 Neuville-sur-Oise, France

Received: 06 May 2021 Accepted: 29 June 2021 Published: 30 June 2021

Cranberry (Vaccinium macrocarpon) dietary supplementation can help prevention of urinary tract infections through the supply of proanthocyanidin-type polyphenols (PAC). The main uropathogenic bacteria are members of the intestinal microbiota. A randomized cross-over experiment was done to investigate whether cranberry dietary supplementation affects concentrations of thermotolerant coliforms, Enterococcus spp. and Lactobacillus spp. in rat faeces. Thirteen rats, housed in individual cages, received successively two diets as pellets during 7 days each: a standard diet without polyphenols and the standard diet supplemented with cranberry powder containing 10.9 mg/100 g of PAC. There was a 7 days wash-out period in between with standard diet without polyphenols. Body weight and feed intake were recorded. Faeces were collected on the last day of treatment, and crushed to count the different bacterial populations using the most probable number method. Thermotolerant coliforms were grown in BGBLB tubes and on MacConkey agar. Enterococcus spp. were grown in Rothe and Litsky broths and on KF Streptococcus agar. Lactobacillus spp. were grown in Man Rogosa Sharpe broth. Body mass gains were not affected by cranberry supplementation. This is consistent with equal food intake, cranberry powder not providing significant energy supplement. Cranberry dietary supplementation was associated with changes in fecal concentrations of thermotolerant coliforms, and Enterococcus spp. in some rats, but did not induce significant changes in bacterial fecal concentrations in a global population of 13 rats. In conclusion, we did not observe any significant effect of dietary cranberry supplementation on the fecal microbiota of Wistars rats for a 7-day diet.
- cranberry,
- diet,
- fecal microbiota,
- Escherichia coli,
- Enterococcus,
- Lactobacillus
Citation: Rayane Chettaoui, Gilles Mayot, Loris De Almeida, Patrick Di Martino. Cranberry (Vaccinium macrocarpon) dietary supplementation and fecal microbiota of Wistar rats[J]. AIMS Microbiology, 2021, 7(2): 257-270. doi: 10.3934/microbiol.2021016

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Abstract

Cranberry (Vaccinium macrocarpon) dietary supplementation can help prevention of urinary tract infections through the supply of proanthocyanidin-type polyphenols (PAC). The main uropathogenic bacteria are members of the intestinal microbiota. A randomized cross-over experiment was done to investigate whether cranberry dietary supplementation affects concentrations of thermotolerant coliforms, Enterococcus spp. and Lactobacillus spp. in rat faeces. Thirteen rats, housed in individual cages, received successively two diets as pellets during 7 days each: a standard diet without polyphenols and the standard diet supplemented with cranberry powder containing 10.9 mg/100 g of PAC. There was a 7 days wash-out period in between with standard diet without polyphenols. Body weight and feed intake were recorded. Faeces were collected on the last day of treatment, and crushed to count the different bacterial populations using the most probable number method. Thermotolerant coliforms were grown in BGBLB tubes and on MacConkey agar. Enterococcus spp. were grown in Rothe and Litsky broths and on KF Streptococcus agar. Lactobacillus spp. were grown in Man Rogosa Sharpe broth. Body mass gains were not affected by cranberry supplementation. This is consistent with equal food intake, cranberry powder not providing significant energy supplement. Cranberry dietary supplementation was associated with changes in fecal concentrations of thermotolerant coliforms, and Enterococcus spp. in some rats, but did not induce significant changes in bacterial fecal concentrations in a global population of 13 rats. In conclusion, we did not observe any significant effect of dietary cranberry supplementation on the fecal microbiota of Wistars rats for a 7-day diet.

Acknowledgments

This study has been supported in part by a grant from the ‘Fondation Université de Cergy-Pontoise’, and by CNRS GDR 2088 ‘BIOMIM’. We thank the ‘département génie biologique’ of the ‘Institut Universitaire de Technologie’ of Cergy-Pontoise for the access to its conventional animal facility.

Authors contributions

Rayane Chettaoui: investigation, analysis and interpretation of the data. Gilles Mayot: methodology, statistical analysis, interpretation of the data, production of illustrations, writing review. Loris De Almeida: investigation. Patrick Di Martino: conceptualization, methodology, supervision, writing original draft.

Conflict of interest

The authors declare no conflict of interest.

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