Research article Special Issues

Studies of rhizobial competitiveness for nodulation in soybean using a non-destructive split-root system

  • Received: 24 February 2017 Accepted: 26 April 2017 Published: 05 May 2017
  • Split-root systems (SRS) constitute an appropriate methodology for studying the relevance of both local and systemic mechanisms that participate in the control of rhizobia-legume symbioses. In fact, this kind of approach allowed to demonstrate the autoregulation of nodulation (AON) systemic response in soybean in the 1980s. In SRS, the plant main root is cut and two lateral roots that emerge from the seedlings after root-tip removal are confined into separate compartments. After several days of growth, these plants have two separate roots that can be inoculated with the same or with different bacteria, at the same or at different times. In this work, we have used a non-destructive SRS to study two different competitiveness relations between rhizobial strains in soybean roots. One of them is the competition for nodulation between two soybean-nodulating rhizobia: the slow-grower Bradyrhizobium japonicum USDA110 and the fast-grower Sinorhizobium fredii HH103. The second is the competitive blocking of S. fredii 257DH4 nodulation in the American soybean Osumi by Sinorhizobium fredii USDA257, which is unable to nodulate American soybeans. Our results showed that the competitiveness relationships studied in this work are mitigated or even avoided when the competitive strains are spatially separated in different compartments containing half-roots from the same plant, suggesting that competitive relations are more related to local plant responses. In our opinion, split-root systems are an appropriate approach to further study competitive relations among rhizobial strains.

    Citation: Ángeles Hidalgo, Francisco-Javier López-Baena, José-Enrique Ruiz-Sainz, José-María Vinardell. Studies of rhizobial competitiveness for nodulation in soybean using a non-destructive split-root system[J]. AIMS Microbiology, 2017, 3(2): 323-334. doi: 10.3934/microbiol.2017.2.323

    Related Papers:

  • Split-root systems (SRS) constitute an appropriate methodology for studying the relevance of both local and systemic mechanisms that participate in the control of rhizobia-legume symbioses. In fact, this kind of approach allowed to demonstrate the autoregulation of nodulation (AON) systemic response in soybean in the 1980s. In SRS, the plant main root is cut and two lateral roots that emerge from the seedlings after root-tip removal are confined into separate compartments. After several days of growth, these plants have two separate roots that can be inoculated with the same or with different bacteria, at the same or at different times. In this work, we have used a non-destructive SRS to study two different competitiveness relations between rhizobial strains in soybean roots. One of them is the competition for nodulation between two soybean-nodulating rhizobia: the slow-grower Bradyrhizobium japonicum USDA110 and the fast-grower Sinorhizobium fredii HH103. The second is the competitive blocking of S. fredii 257DH4 nodulation in the American soybean Osumi by Sinorhizobium fredii USDA257, which is unable to nodulate American soybeans. Our results showed that the competitiveness relationships studied in this work are mitigated or even avoided when the competitive strains are spatially separated in different compartments containing half-roots from the same plant, suggesting that competitive relations are more related to local plant responses. In our opinion, split-root systems are an appropriate approach to further study competitive relations among rhizobial strains.


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    [1] Peix A, Ramírez-Bahena MH, Velázquez E, et al. (2015) Bacterial associations with legumes. Crit Rev Plant Sci 34: 7–42.
    [2] Oldroyd GE (2013) Speak, friend, and enter: signalling systems that promote beneficial symbiotic associations in plants. Nat Rev Microbiol 11: 252–263. doi: 10.1038/nrmicro2990
    [3] Suzaki T, Kawaguchi M (2014) Root nodulation: a developmental program involving cell fate conversion triggered by symbiotic bacterial infection. Curr Opin Plant Biol 21: 16–22. doi: 10.1016/j.pbi.2014.06.002
    [4] López-Baena FJ, Ruiz-Sainz JE, Rodríguez-Carvajal MA, et al. (2016) Bacterial molecular signals in the Sinorhizobium fredii-soybean symbiosis. Int J Mol Sci 17: E755.
    [5] Graham PH, Vance CP (2000) Nitrogen fixation in perspective: an overview of research and extension needs. Field Crops Res 65: 93–106. doi: 10.1016/S0378-4290(99)00080-5
    [6] Graham PH, Vance CP (2003) Legumes: importance and constraints to greater use. Plant Physiol 131: 872–877. doi: 10.1104/pp.017004
    [7] Lindström K, Murwira M, Willems A, et al. (2010) The biodiversity of beneficial microbe-host mutualism: the case of rhizobia. Res Microbiol 161: 453–463. doi: 10.1016/j.resmic.2010.05.005
    [8] Rodríguez-Navarro DN, Margaret-Oliver I, Albareda-Contreras M, et al. (2011) Soybean interactions with soil microbes, agronomical and molecular aspects. Agronomy Sust Developm 31: 173–190.
    [9] Curatti L, Rubio LM (2014) Challenges to develop nitrogen-fixing cereals by direct nif-gene transfer. Plant Sci 225: 130–137. doi: 10.1016/j.plantsci.2014.06.003
    [10] Dowling DN, Broughton WJ (1986) Competition for nodulation of legumes. Annu Rev Microbiol 40: 131–157. doi: 10.1146/annurev.mi.40.100186.001023
    [11] Triplett EW, Sadowsky MJ (1992) Genetics of competition for nodulation of legumes. Annu Rev Microbiol 46: 399–428. doi: 10.1146/annurev.mi.46.100192.002151
    [12] Friesen ML (2012) Widespread fitness alignment in the legume-rhizobium symbiosis. New Phytol 194: 1096–1111. doi: 10.1111/j.1469-8137.2012.04099.x
    [13] Ruiz-Sainz JE, Zhou JC, Rodriguez-Navarro DN, et al. (2005) Soybean cultivation and BNF in China, In: Werner, D., Newton, W.E., Editors., Nitrogen fixation in agriculture, forestry, ecology, and the environment, Dordrecht: Springer, 67–87.
    [14] Thilakarathna MS, Raizada MN (2017) A meta-analysis of the effectiveness of diverse rhizobia inoculants on soybean traits under field conditions. Soil Biol Biochem 105: 177–196. doi: 10.1016/j.soilbio.2016.11.022
    [15] Delamuta JR, Ribeiro RA, Ormeño-Orrillo E, et al. (2013) Polyphasic evidence supporting the reclassification of Bradyrhizobium japonicum group Ia strains as Bradyrhizobium diazoefficiens sp. nov. Int J Syst Evol Microbiol 63: 3342–3351. doi: 10.1099/ijs.0.049130-0
    [16] Buendía-Clavería AM, Rodríguez-Navarro DN, Santamaría-Linaza C, et al. (1994) Evaluation of the symbiotic properties of Rhizobium fredii in European Soils. Syst Appl Microbiol 17: 155–160.
    [17] Yang SS, Bellogín RA, Buendía A, et al. (2001) Effect of pH and soybean cultivars on the quantitative analyses of soybean rhizobia populations. J Biotechnol 91: 243–255. doi: 10.1016/S0168-1656(01)00340-6
    [18] Pueppke SG, Broughton WJ (1999) Rhizobium sp. strain NGR234 and R. fredii USDA257 share exceptionally broad, nested host ranges. Mol Plant Microbe Interact 12: 293–318.
    [19] Annapurna K, Krishnan HB (2003) Molecular aspects of soybean cultivar-specific nodulation by Sinorhizobium fredii USDA257. Indian J Exp Biol 41: 1114–1123.
    [20] Yasuda M, Miwa H, Masuda S, et al. (2016) Effector-triggered immunity determines host genotype-specific incompatibility in legume-Rhizobium symbiosis. Plant Cell Physiol 57: 1791–1800. doi: 10.1093/pcp/pcw104
    [21] Meinhardt LW, Krishnan HB, Balatti PA, et al. (1993) Molecular cloning and characterization of a sym plasmid locus that regulates cultivar-specific nodulation of soybean by Rhizobium fredii USDA257. Mol Microbiol 9: 17–29. doi: 10.1111/j.1365-2958.1993.tb01665.x
    [22] Rodríguez-Navarro DN, Rodríguez-Carvajal MA, Acosta-Jurado S, et al. (2014) Structure and biological roles of Sinorhizobium fredii HH103 exopolysaccharide. PLoS One 9: e115391.
    [23] Vinardell JM, Ollero FJ, Hidalgo A, et al. (2004) NolR regulates diverse symbiotic signals of Sinorhizobium fredii HH103. Mol Plant-Microbe Interact 17: 676–685. doi: 10.1094/MPMI.2004.17.6.676
    [24] Vinardell JM, López-Baena FJ, Hidalgo A, et al. (2004) The effect of FITA mutations on the symbiotic properties of Sinorhizobium fredii varies in a chromosomal-background-dependent manner. Arch Microbiol 181: 144–154. doi: 10.1007/s00203-003-0635-3
    [25] Vincent JM (1970) Appendix III. The modified Fåhraeus slide technique, In: Vincent JM, editor, A Manual for the Practical Study of Root Nodule Bacteria, Oxford: Blackwell Scientific, 144–145.
    [26] Larrainzar E, Gil-Quintana E, Arrese-Igor C, et al. (2014) Split-root systems applied to the study of the legume-rhizobial symbiosis: what have we learned? J Integr Plant Biol 56: 1118–1124
    [27] Hidalgo A, Ruiz-Sainz JE, Vinardell JM, A new, non-destructive, split-root system for studies with soybean, In: Medina-Morillas C, Lopez Baena J, Editors, Host-Pathogen Interactions: Methods and Protocols, Springer.
    [28] Dowdle SF, Bohlool BB (1987) Intra- and inter-specific competition in Rhizobium fredii and Bradyrhizobium japonicum as indigenous and introduced organisms. Can J Microbiol 33: 990–995. doi: 10.1139/m87-174
    [29] McLoughlin TJ, Owens PA, Scott A (1985) Competition studies with fast-growing Rhizobium japonicum strains. Can J Microbiol 31: 220–223. doi: 10.1139/m85-042
    [30] Staehelin C, Krishnan HB (2015) Nodulation outer proteins: double-edged swords of symbiotic rhizobia. Biochem J 470: 263–274. doi: 10.1042/BJ20150518
    [31] Reid DE, Ferguson BJ, Hayashi S, et al. (2011) Molecular mechanisms controlling legume autoregulation of nodulation. Ann Bot 108: 789–795. doi: 10.1093/aob/mcr205
    [32] Kosslak RM, Bohlool BB (1985) Suppression of nodule development of one side of a split-root system of soybeans caused by prior inoculation of the other side. Plant Physiol 75: 125–130.
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