Investigating the role of caveolin-2 in prostate cancer cell line

Jin-Yih Low; Helen D. Nicholson; Jin-Yih Low; Helen D. Nicholson

doi:10.3934/molsci.2017.1.82

AIMS Molecular Science

2017, Volume 4, Issue 1: 82-88. doi: 10.3934/molsci.2017.1.82

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Investigating the role of caveolin-2 in prostate cancer cell line

Jin-Yih Low ^,,
Helen D. Nicholson

Department of Anatomy, Otago School of Medical Sciences, University of Otago, Dunedin, New Zealand

Received: 15 October 2016 Accepted: 16 February 2016 Published: 20 February 2017

Prostate cancer is a worldwide problem. While the role of caveolin-1 has been extensively studied, little is known about the role of caveolin-2 (CAV2) in prostate cancer. Up-regulation of CAV2 in androgen independent PC3 cells compared to normal prostate cell line and androgen dependent prostate cancer cell lines has been observed. Recent studies suggest that up-regulation of CAV2 plays an important role in androgen independent prostate cancer. This study investigates whether CAV2 is important in mediating the aggressive phenotypes seen in androgen independent prostate cancer cells. The androgen independent prostate cancer cell line, PC3 was used that has been shown to express CAV2, and CAV2 knock down was performed using siRNA system. Changes to cell number, migration and invasion were assessed after knocking down CAV2. Our results showed that down-regulating CAV2 resulted in reduced cell numbers, migration and invasion in PC3 cells. This preliminary study suggests that CAV2 may act to promote malignant behavior in an androgen independent prostate cancer cell line. Further studies are required to fully elucidate the role of CAV2 in androgen independent prostate cancer.
- caveolae,
- caveolin-2,
- migration,
- invasion
Citation: Jin-Yih Low, Helen D. Nicholson. Investigating the role of caveolin-2 in prostate cancer cell line[J]. AIMS Molecular Science, 2017, 4(1): 82-88. doi: 10.3934/molsci.2017.1.82

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Abstract

Prostate cancer is a worldwide problem. While the role of caveolin-1 has been extensively studied, little is known about the role of caveolin-2 (CAV2) in prostate cancer. Up-regulation of CAV2 in androgen independent PC3 cells compared to normal prostate cell line and androgen dependent prostate cancer cell lines has been observed. Recent studies suggest that up-regulation of CAV2 plays an important role in androgen independent prostate cancer. This study investigates whether CAV2 is important in mediating the aggressive phenotypes seen in androgen independent prostate cancer cells. The androgen independent prostate cancer cell line, PC3 was used that has been shown to express CAV2, and CAV2 knock down was performed using siRNA system. Changes to cell number, migration and invasion were assessed after knocking down CAV2. Our results showed that down-regulating CAV2 resulted in reduced cell numbers, migration and invasion in PC3 cells. This preliminary study suggests that CAV2 may act to promote malignant behavior in an androgen independent prostate cancer cell line. Further studies are required to fully elucidate the role of CAV2 in androgen independent prostate cancer.

References

[1]	Anderson RG (1998) The caveolae membrane system. Annu Rev Biochem 67: 199-225. doi: 10.1146/annurev.biochem.67.1.199
[2]	Parat MO (2009) The biology of caveolae: achievements and perspectives. Int Rev Cell Mol Biol 273: 117-162. doi: 10.1016/S1937-6448(08)01804-2
[3]	Scherer PE, Okamoto T, Chun M, et al. (1996) Identification, sequence, and expression of caveolin-2 defines a caveolin gene family. Proc Natl Acad Sci U S A 93: 131-135. doi: 10.1073/pnas.93.1.131
[4]	Li S, Galbiati F, Volonte D, et al. (1998) Mutational analysis of caveolin-induced vesicle formation. Expression of caveolin-1 recruits caveolin-2 to caveolae membranes. FEBS Lett 434: 127-134.
[5]	Razani B, Wang XB, Engelman JA, et al. (2002) Caveolin-2-deficient mice show evidence of severe pulmonary dysfunction without disruption of caveolae. Mol Cell Biol 22: 2329-2344. doi: 10.1128/MCB.22.7.2329-2344.2002
[6]	Das K, Lewis RY, Scherer PE, et al. (1999) The membrane-spanning domains of caveolins-1 and -2 mediate the formation of caveolin hetero-oligomers. Implications for the assembly of caveolae membranes in vivo. J Biol Chem 274: 18721-18728.
[7]	Rybin VO, Grabham PW, Elouardighi H, et al. (2003) Caveolae-associated proteins in cardiomyocytes: caveolin-2 expression and interactions with caveolin-3. Am J Physiol Heart Circ Physiol 285: H325-332. doi: 10.1152/ajpheart.00946.2002
[8]	Razani B, Woodman SE, Lisanti MP (2002) Caveolae: from cell biology to animal physiology. Pharmacol Rev 54: 431-467. doi: 10.1124/pr.54.3.431
[9]	Savage K, Leung S, Todd SK, et al. (2008) Distribution and significance of caveolin 2 expression in normal breast and invasive breast cancer: an immunofluorescence and immunohistochemical analysis. Breast Cancer Res Treat 110: 245-256. doi: 10.1007/s10549-007-9718-1
[10]	Elsheikh SE, Green AR, Rakha EA, et al. (2008) Caveolin 1 and Caveolin 2 are associated with breast cancer basal-like and triple-negative immunophenotype. Br J Cancer 99: 327-334. doi: 10.1038/sj.bjc.6604463
[11]	Fong A, Garcia E, Gwynn L, et al. (2003) Expression of caveolin-1 and caveolin-2 in urothelial carcinoma of the urinary bladder correlates with tumor grade and squamous differentiation. Am J Clin Pathol 120: 93-100. doi: 10.1309/292NHAYNWAVREJ37
[12]	Ando T, Ishiguro H, Kimura M, et al. (2007) The overexpression of caveolin-1 and caveolin-2 correlates with a poor prognosis and tumor progression in esophageal squamous cell carcinoma. Oncol Rep 18: 601-609.
[13]	Sugie S, Mukai S, Yamasaki K, et al. (2015) Significant Association of Caveolin-1 and Caveolin-2 with Prostate Cancer Progression. Cancer Genomics Proteomics 12: 391-396.
[14]	Gould ML, Williams G, Nicholson HD (2010) Changes in caveolae, caveolin, and polymerase 1 and transcript release factor (PTRF) expression in prostate cancer progression. Prostate 70: 1609-1621. doi: 10.1002/pros.21195
[15]	Parsons BD, Schindler A, Evans DH, et al. (2009) A direct phenotypic comparison of siRNA pools and multiple individual duplexes in a functional assay. PLoS One 4: e8471. doi: 10.1371/journal.pone.0008471
[16]	Van den Eynden GG, Van Laere SJ, Van der Auwera I, et al. (2006) Overexpression of caveolin-1 and -2 in cell lines and in human samples of inflammatory breast cancer. Breast Cancer Res Treat 95: 219-228. doi: 10.1007/s10549-005-9002-1
[17]	Yamasaki T, Seki N, Yoshino H, et al. (2013) MicroRNA-218 Inhibits Cell Migration and Invasion in Renal Cell Carcinoma Through Targeting Caveolin-2 Involved in Focal Adhesion Pathway. J Urol 190: 1059-1068. doi: 10.1016/j.juro.2013.02.089

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