Mini review Topical Sections

Application of biosurfactants in environmental biotechnology; remediation of oil and heavy metal

  • Many toxic substances have been introduced into environment through human activities. These compounds are danger to human health when they are ultimately or immediately in contact with soil particles. A conventional method to reduce, degrade and remove these substances is associated with some risk. In recent years, microorganisms have proved a unique role in the degradation and detoxification of polluted soil and water environments and, this process has been termed bio reclamation. The diversity of bioemulsifiers/biosurfactants makes them an attractive group and important key roles in various fields of industrial as well as biotechnological applications such as enhanced oil recovery, biodegradation of pollutants, and pharmaceutics. Environmental application of microbial surfactant has been shown as a promising due to solubilization of low solubility compounds, low toxicity observed and efficacy in improving biodegradation. However, it is important to note that full scale tests and more information is require to predict the behavior and model of surfactant function on the remediation process with biosurfactants. The purpose of this review is to describe the state of art in the potential applications of biosurfactants in remediation of environmental pollution caused by oil and heavy metal.

    Citation: Mohammed Maikudi Usman, Arezoo Dadrasnia, Kang Tzin Lim, Ahmad Fahim Mahmud, Salmah Ismail. Application of biosurfactants in environmental biotechnology; remediation of oil and heavy metal[J]. AIMS Bioengineering, 2016, 3(3): 289-304. doi: 10.3934/bioeng.2016.3.289

    Related Papers:

    [1] Chiara Ceresa, Francesco Tessarolo, Devid Maniglio, Iole Caola, Giandomenico Nollo, Maurizio Rinaldi, Letizia Fracchia . Inhibition of Candida albicans biofilm by lipopeptide AC7 coated medical-grade silicone in combination with farnesol. AIMS Bioengineering, 2018, 5(3): 192-208. doi: 10.3934/bioeng.2018.3.192
    [2] Chiara Ceresa, Maurizio Rinaldi, Letizia Fracchia . Synergistic activity of antifungal drugs and lipopeptide AC7 against Candida albicans biofilm on silicone. AIMS Bioengineering, 2017, 4(2): 318-334. doi: 10.3934/bioeng.2017.2.318
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    [4] Ana Meireles, Ana L. Gonçalves, Inês B. Gomes, Lúcia Chaves Simões, Manuel Simões . Methods to study microbial adhesion on abiotic surfaces. AIMS Bioengineering, 2015, 2(4): 297-309. doi: 10.3934/bioeng.2015.4.297
    [5] Merily Horwat, Meggie Tice, Birthe V. Kjellerup . Biofilms at work: Bio-, phyto- and rhizoremediation approaches for soils contaminated with polychlorinated biphenyls. AIMS Bioengineering, 2015, 2(4): 324-334. doi: 10.3934/bioeng.2015.4.324
    [6] Sara R Robertson, Robert JC McLean . Beneficial biofilms. AIMS Bioengineering, 2015, 2(4): 437-448. doi: 10.3934/bioeng.2015.4.437
    [7] Yung-Hua Li, Xingxing Huang, Xiao-Lin Tian . Recent advances in dental biofilm: impacts of microbial interactions on the biofilm ecology and pathogenesis. AIMS Bioengineering, 2017, 4(3): 335-350. doi: 10.3934/bioeng.2017.3.335
    [8] Lucie Semenec, Ashley E Franks . Delving through electrogenic biofilms: from anodes to cathodes to microbes. AIMS Bioengineering, 2015, 2(3): 222-248. doi: 10.3934/bioeng.2015.3.222
    [9] Frank B Dazzo, Youssef G Yanni, Ashley Jones, Abdelgawad Y Elsadany . CMEIAS bioimage informatics that define the landscape ecology of immature microbial biofilms developed on plant rhizoplane surfaces. AIMS Bioengineering, 2015, 2(4): 469-486. doi: 10.3934/bioeng.2015.4.469
    [10] Joe A. Lemire, Marc A. Demeter, Iain George, Howard Ceri, Raymond J. Turner . A novel approach for harnessing biofilm communities in moving bed biofilm reactors for industrial wastewater treatment. AIMS Bioengineering, 2015, 2(4): 387-403. doi: 10.3934/bioeng.2015.4.387
  • Many toxic substances have been introduced into environment through human activities. These compounds are danger to human health when they are ultimately or immediately in contact with soil particles. A conventional method to reduce, degrade and remove these substances is associated with some risk. In recent years, microorganisms have proved a unique role in the degradation and detoxification of polluted soil and water environments and, this process has been termed bio reclamation. The diversity of bioemulsifiers/biosurfactants makes them an attractive group and important key roles in various fields of industrial as well as biotechnological applications such as enhanced oil recovery, biodegradation of pollutants, and pharmaceutics. Environmental application of microbial surfactant has been shown as a promising due to solubilization of low solubility compounds, low toxicity observed and efficacy in improving biodegradation. However, it is important to note that full scale tests and more information is require to predict the behavior and model of surfactant function on the remediation process with biosurfactants. The purpose of this review is to describe the state of art in the potential applications of biosurfactants in remediation of environmental pollution caused by oil and heavy metal.


    1. Introduction

    Biosurfactants comprise a wide range of surface-active structurally different organic compounds produced by numerous prokaryotic and eukaryotic microorganisms. These compounds are generally extracellularly excreted or localized on microbial cell surfaces andare made of amphiphilic molecules in which the hydrophobic moiety may include an acid, mono-, di- or polysaccharides, peptide cations, or anions, while the hydrophobic moiety may be composed of saturated or unsaturated fatty acid or hydrocarbon chains [1]. Biosurfactants are generally grouped according to their chemical structure, molecular weight, and mode of action. The best studied biosurfactants are glycolipids such as rhamnolipids, trehalolipids, sophorolipids and mannosylerythritol lipids and lipopeptides such as surfactin and fengycin.

    These compounds orientation and behaviour on surfaces and interphases confers to these compounds a range of properties, such as the ability to decrease surface and interfacial tension of liquids and the formation of microemulsions and micelles between different phases [2,3].Biosurfactants tend to aggregate in heterogeneous systems and at interfaces or boundaries and to form molecular interfacial films that alters the original properties of these surfaces.

    In the past twenty years, a large volume of research activity has been dedicated to biosurfactants as potential replacement for synthetic surfactants in many industrial and environmental applications such as detergent, textile, paint, cosmetic, bioremediation, enhanced oil recovery, food, agrochemical fields and several commercial products have already been manufactured [4].

    More recently, numerous investigations have led to the discovery of several interesting biological and chemical properties of biosurfactants and several pharmaceutical and medical applications have been envisaged [5,6]. In particular, the ability to disturb membranes integrity destabilizing them and permeability leading to metabolite leakage and cell lysis [7,8,9,10], as well as their propensity to partition at the interfaces, modifying surface properties and thus affecting microorganisms adhesion, which are important functions for antimicrobial and anti-biofilm applications [11]. Additionally, some experimental results have suggested that they are non-toxic or less toxic when compared to synthetic surfactants [12,13], a valuable characteristic for biomedical applications.

    In this review, we focus on recent advances on biosurfactants as antimicrobial and anti-adhesive compounds, with a brief overview on the latest outcome on innovative therapeutic and biotechnological applications.

    2. Biosurfactants as Biological Control Agents

    The urgent need for new antimicrobial compounds nowadays remains of major concern due to the newly emerging pathogens and other conventional ones the majority of which have become almost insensitive to existing antibiotics [14]. Microbial metabolites are also known as a major source of compounds categorized with potent biological activities and, among these, some biosurfactants have been described as adjuvants or potential alternatives to antimicrobial agents and synthetic medicines [11]. Moreover, in addition to their ability to modulate the interaction of cells with surfaces, biosurfactants are able to interfere with microbial adhesion and biofilm formation, an important and frequently hazardous manifestations on medical devices, especially as such biofilms contain bacterial strains that often become highly resistant to adverse environmental challenges and antibiotics [15,16]. It would be useful therefore to increase the efficacy of known biocides and antibiotics with alternative strategies aimed at reducing the biofilm populations and decreasing bacterial adhesion to medical devices surfaces.

    2.1. Mechanisms of action

    Establishing the functional mechanisms of actions for biosurfactants is of immense importance to assist the discovery of interesting applications. Among biosurfactants, lipopeptides and glycolipids have the most potent antimicrobial activity and represent an important source for the identification of new antibiotics.

    2.1.1. Lipopeptide type compounds

    The antimicrobial activities of lipopeptides, such as surfactin [17] and fengycin [18], are due to their ability to self-associate and form micellular aggregates or pore-bearing channels inside the lipid membrane. Due to these properties, lipopeptides usually cause membrane disruption, increased membrane permeability, metabolite leakages and cell lysis. Furthermore, membrane structure changes and disruption of protein conformations alter vital membrane functions including energy generation and transport [19,20]. Studies carried out on daptomycin showed that the lipopeptide oligomer binding which can be Ca2+ dependent often leads to the formation of pores within the membranes [21]. These pores may lead to membrane disruption and cell death as a result of transmembrane ion influxes, including Na+ and K+ [22]. The bactericidal activity of lipopeptides increases with the presence of a lipid tail length of 10-12 carbons atoms whereas an enhanced antifungal activity is exhibited in lipopeptides with a lipid tail length of 14 or 16 carbon atoms [20]. In addition, due to the difficulty of the target cells to reorganize their membranes, the ability to develop resistant strains is significantly diminished [22].

    Surfactin, which is often described as a powerful biosurfactant has the capability to disturb the integrity and permeability of membranes destabilizing them. In fact, surfactin generates physical structural changes in the membrane or disrupts protein conformations, which can change some central membrane functions such as the generation of energy and transport [7,8,9,23]. One of the crucial steps for cellular membrane leakage and destabilization is the dimerization of surfactin into its bilayer [17]. Surfactin incorporation into membranes, in vitro, leads to the dehydration of the head groups of the phospholipid and bilayer instability due to the perturbation of lipid packing which ultimately leads to the alteration and distortion of the membrane barrier properties [17]. For antiviral activity, surfactin acts directly on the mainly lipidic viral envelope causing leakages or complete disintegration of the envelope exposing the capsid of the virus particles, which leads to loss infectivity.

    Mechanisms of action and activity of other lipopeptides have recently been reviewed by Cochrane and Vederas [24]. Polymyxins primarily exert their strong bactericidal effect against Gram-negative bacteria through the binding of the lipid A component of lipopolysaccharide (LPS) and disruption of the outer membrane, followed by the permeabilization and disruption of the inner membrane [25,26]. Octapeptins A and B display broad-spectrum activity against both Gram-positive and Gram-negative bacteria and have also antimicrobial activity against some filamentous fungi, protozoa and yeasts due to their ability to disrupt the cytoplasmic membrane. The iturin family compounds exerts fungicidal action through the interaction with sterol components in the fungal membrane, leading to an increase in K+ permeability [27]. It is generally believed that the first step in the interaction between a surfactant and a bacterial cell consists of an ionic adsorption to the bacterial cell wall which is followed by damage to cell membrane leading to inactivation of metabolic processes and cell lysis. The role or preferential attachment of surfactants to the Gram-positive or negative cell wall may be a potential explanation to their selective activity on either types of cells but yet remains to be established.

    2.1.2. Glycolipidic type compounds

    Concerning glycolipidic compounds mode of action, Sotirova et al. [28] demonstrated that the exposure of Pseudomonas aeruginosa to rhamnolipids causes a multi-component response of the bacterial cells characterized by a reduction of total cellular LPS content, an increase in cell hydrophobicity and changes in membrane proteins and surface morphology. In the same way, antimicrobial activity of sophorolipids involves mechanisms that cause destabilization and alteration of the permeability of the cellular membrane [29]. Furthermore, Ortiz et al. [7] have recently reported on the interactions of bacterial biosurfactants trehalose lipids with phosphatidylserine and phosphatidylethanolamine membranes. Their results demonstrated that trehalose lipids, when incorporated into the bilayers, increased hydrocarbon chain conformational disorder and decreased the hydration of the interfacial region of the bilayer, leading to structural perturbations that might affect membranes functions.

    The ability to reduce microbial cells adhesion to surfaces, thus limiting biofilm formation, is another well-known property of biosurfactants. Both numbers and initial deposition rates of microorganisms adhering to surfaces are determined by complex interactions of hydrophobicity (interfacial free energies), the presence of specific receptor sites on the microbial cell surfaces, electrostatic interactions and types of biosurfactants produced. In particular, biofilm formation on solid surfaces is generally directly proportional to the hydrophobicity of the surface, as long as the suspended medium is a simple buffer [30]. Microbial adhesion on hydrophobic substrates (e.g. silicone rubber) was speculated to be related to the removal of interfacial water between microorganism and interacting surfaces, which facilitates closer approach and adhesion [31]. The authors also advocated that biosurfactants reduce hydrophobic interactions which decrease surface hydrophobicity that ultimately hinders microbial adhesion to surfaces and subsequently interferes with biofilms development.

    2.2. Antimicrobial activity of biosurfactants

    2.2.1. Biosurfactant activity against human pathogenic bacteria and fungi

    The most commonly reported class of biosurfactants with antimicrobial activity, are lipopeptides [24]. Antimicrobial lipopeptides include surfactin, iturin, fengycin, mycosubtilins and bacillomycins produced by Bacillus subtilis, [32], cyclic lipopeptides such as daptomycin, from Streptomyces roseosporus [33], polymyxin B, pumilacidin and lichenysin produced by Bacillus polymyxa, Bacillus pumilus and Bacillus licheniformis, respectively [34] and finally viscosin, from Pseudomonads [35]. Glycolipids, have also been reported to display antimicrobial activities, in particular, rhamnolipids from P. aeruginosa [36], sophorolipids from Candida bombicola [37,38], mannosylerythritol lipids (MEL-A and MEL-B) from Candida antarctica [39].

    Ghribi et al. [40] reported a broad spectrum antimicrobial activity against bacteria and fungi and effects against multidrug-resistant microbial strains for a biosurfactant produced by B. subtilis SPB1. The compound showed less activity against Gram-negative bacilli and higher activity against Gram-positive cocci with particularly significant effects against Enterococcus faecalis.

    Using strain Paenibacillus elgii B69, Ding et al. [41] isolated two lipopeptide antibiotics, pelgipeptins C and D which were active against pathogenic Candida fungal strains and a number of Gram-negative and Gram-positive bacteria. In particular, pelgipeptin D exhibited effective rapid bactericidal action against a methicillin resistant strain of Staphylococcus aureus and with an intraperitoneal LD50 acute toxicity test values slightly higher than polymyxin B a structurally related antimicrobial agent. Tabbene et al. [42] also reported three anti-Candida albicans compounds (a1, a2 and a3) derived from B. subtilis B38 and resembling bacillomycin D-like lipopeptides. Compound a3 had strongest fungicidal activity exceeding amphotericin B activity against a pathogenic strain of C. albicans sp. 311 isolated from fingernail.

    More recently, a lipopeptide produced by B. licheniformis M104 were investigated as antimicrobial agent against Gram-positive bacteria (B. subtilis, B. thuringiensis, B. cereus, S. aureus and Listeria monocytogenes), Gram-negative bacteria (P. aeruginosa, Escherichia coli, Salmonella typhimurium, Proteus vulgaris, Klebsiella pneumoniae) and C. albicans [43]. All the tested microorganisms, with the exception of L. monocytogenes and K. pneumoniae, were affected by the biosurfactant and S. aureus was the most susceptible. The antimicrobial effect of the lipopeptide was time and concentration-dependent. The maximum inhibitory activity was observed at a concentration of 48 μg ml−1 after 12h of treatment. The lipopeptide 6-2 produced by Bacillus amyloliquefaciens was also found to have interesting antifungal activity against C. albicans, Metschnikowia bicuspidate, Candida tropicalis, Yarrowia lipolytica and Saccharomyces cerevisiae [44]. Scanning electronic microscopy revealed the mode of action of lipopeptide 6-2 against C. albicans showing the presence of invaginations in the cell wall, disruption of the whole cells followed by the loss of integrity of the cell wall. They also reported that lipopeptide 6-2 biosurfactant damaged the plasma membranes of C. albicans protoplast leading to its lysis [44].

    Very recently, Sharma et al. [45] purified and characterized a novel lipopeptide from Streptomyces amritsarensis sp. The antimicrobial activity of the biosurfactant was evaluated on a broad spectrum of bacteria and fungi. The MIC values of purified lipopeptide against B. subtilis, Staphylococcus epidermidis, Mycobacterium smegmatis strains and a methicillin resistant S. aureus (MRSA) were reported to be 10, 15, 25 and 45 μg ml−1, respectively. No activity against any of the tested Gram-negative bacteria and against fungi was observed. The surface-active lipopeptide heat stability test established that exposure to 100 °C or 121 °C for 15min reduced the antimicrobial action by 13.7% and 18.2% respectively. It also showed both non-cytotoxic and non-mutagenic properties, which are important prerequisite for drug development.

    Liang et al. [46] analysed the antimicrobial effect of a biosurfactant obtained by cultivating the strain Paenibacillus macerans TKU029 in a medium with 2% (w/v) squid pen powder as carbon/nitrogen source. The purified TKU029 biosurfactant displayed significant inhibitory effect on E. coli and S. aureus at concentrations of 2 and 1.5 mg ml-1 respectively and showed good antifungal activity against Fusarium oxysporum and Aspergillus fumigatus.

    Serrawettin W1, first described as serratamolide [47], is reported to be an antimicrobial, antitumor and plant-protecting molecule, making this biosurfactant an interesting candidate for cosmetics or pharmaceuticals applications [48,49,50]. Very recently, Kadouri and Shanks [51] demonstrated the inhibitory activity of this compound against MRSA strains and other Gram-positive organisms. Furthermore, despite the cytotoxic activity of serratamolide, the authors suggest that bacterial aminolipids may be a source for future antibiotics effective against MRSA and may play a role in microbial competition.

    Samadi et al. [52] evaluated some biological activities of mono and di-rhamnolipids produced by P. aeruginosa MN1 and reported that the mono-rhamnolipid fraction was a more potent antibacterial agent than the di-rhamnolipid fraction, in particular, against Gram-positive bacteria that were inhibited at 25 μg ml−1 concentration. Moreover, the rhamnolipids remarkably enhanced oxacillin inhibitory effects against MRSA strains lowering its minimum inhibitory concentrations to 3.12-6.25 μg ml−1.

    Rhamnolipids were also examined to evaluate their antimicrobial potential against alone and when combined with nisin (a food preservative) against two wild-type strains of L. monocytogenes [53]. Rhamnolipids alone had an MIC values ranging from 78 to 2500 mg ml−1, which was significantly reduced when in combination of nisin showing strong synergistic effect against L. monocytogenes isolates.

    In other works Luna et al. [54] and Rufino et al. [55] demonstrated antimicrobial activity of two biosurfactants derived respectively from Candida sphaerica UCP0995 and Candida lipolytica UCP 0988, known to produce sophorolipids (SL), against Gram-positive strains such as Streptococcus mutans, Streptococcus sanguis, Streptococcus agalactiae, S. epidermidis, Streptococcus oralis, and against C. albicans. Synergistic effects for sophorolipids biosurfactants (SL) with selected antibiotics were also reported by Joshi-Navare and Prabhune [56]. A strain of S. aureus was not totally inhibited by tetracycline at the concentration of 15 µg ml-1 after 6h exposure but was totally inhibited within 4h when combined with sophorolipids (at 300 µg ml−1). Similarly, Cefaclor antibiotic showed better effects on E. coli when administered in combination with SL. Scanning electron microscopy revealed that the cells treated with mixtures of SL and antibiotics were characterized by cell membrane damage and pore formation, leading to enhanced leakage of the cytoplasmic contents and accumulation of cell debris. Similarly, a glycolipid biosurfactant from Halomonas sp BS4, containing 1,2-Ethanediamine N, N, N’, N’- tetra and (Z)-9-octadecenamide, showed antibacterial activity against S. aureus, K. pneumoniae, Streptococcus pyrogenes and Salmonella typhi and antifungal activity against Aspergillus niger, Fusarium sp, Aspergillus flavus and Trichophyton rubrum [57].

    In spite of the high number of publication describing the antimicrobial activity of biosurfactants and of patents related to their usage, real applications in pharmaceutical, biomedical and health improvement related industries remains quite limited [4]. Some lipopeptides have reached a commercial antibiotic status, like echinocandins [58], micafungin [59], anidulafungin [60] and daptomycin [61]. Daptomycin a branched cyclic lipopeptide isolated from cultures of S. roseosporus and produced by Cubist Pharmaceuticals under the name Cubicin® [61], was approved in 2003 for skin infections treatment caused by MRSA and other Gram-positive pathogens and in 2006 for the treating endocarditis and bacteraemia usually caused by S. aureus. Daptomycin had also been reported to displays strong antibacterial activity against other important pathogens, such as penicillin-resistant Streptococcus pneumoniae, coagulase-negative Staphylococci (CNS), glycopeptide-intermediate-susceptible S. aureus (GISA) and vancomycin resistant Enterococci (VRE) [62].

    Other lipopeptides such as micafungin, echinocandins and anidulafungin are low-toxic synthetically modified lipopeptides, usually obtained from the fermentation broths of various fungi [63]. Echinocandins can inhibit fungal cell wall formation particularly against Aspergillus spp. Candida spp. and Pneumocystis carinii [64]. The first licensed echinocandin was caspofungin; approved since 2001 for the treatment of invasive aspergillosis, esophageal and invasive candidiasis particularly in difficult to treat cases [58]. Micafungin has been used to combat Candida and Aspergillus invasive infections in immune compromised children [59] whereas anidulafungin in the treatment of all forms of candidiasis [60]. Other lipopeptides suitable for the prevention or treatment of microbial infections have also been described as suitable antimicrobial agents with pharmaceutical applications [65]. For example, viscosin lipopeptides and congeners have been patented as therapeutic compounds capable of inhibiting Trypanosoma cruzi, Mycobacterium tuberculosis and a Herpes simplex virus [66].

    2.2.2. Antiviral activity of biosurfactants

    Antiviral activity of biosurfactants has also been observed, mostly against enveloped viruses, such as herpes viruses and retroviruses compared to non-enveloped viruses. This is believed to be due to the inhibitory action and physico-chemical interactions between the surfactants and the virus envelope [67]. Antiviral activity against bursal disease virus and newcastle disease virus was observed for lipopeptides produced by B. subtilis fmbj [68]. Similarly, sophorolipids and rhamnolipids alginate complex showed antiviral activity against HIV, human immunodeficiency virus [69] and herpes simplex viruses [70] respectively.

    2.3. Biosurfactants role in biofilms and as anti-adhesives

    The continuous increase in the use of medical devices is often associated with tangible risk of infectious complications, endocarditis, metastatic infections, septic thrombophlebitis and sepsis. These microbial infections are usually due to the formation of biofilms, complexbiological structures adhering to the medical device consisting of a sessile and multicellular community encapsulated in a hydrated matrix of proteins and polysaccharides. Once a mature biofilm is established, the bacterial strains embedded within become greatly resistant to both antimicrobial agents [71] and host immune response. The Gram-positive bacteria S. aureus, S. epidermidis, E. faecalis, constitute >50% of the species isolated from patients with infections related to medical device biofilms such as catheter associated infections. P. aeruginosa, Candida spp. and uropathogenic E. coli are th e remaining causal agents. Similarly, orthopedic metallic prostheses are associated with a significant risk of infection [72,73].

    Coating medical surfaces with antimicrobial agents are the most common current biofilm preventive strategies, a process not always successful [74]. Surface modification strategies based on plasma, UV and corona discharge treatment of typical catheter materials, such as silicone and polyurethanes, have been developed with the aim to increase material hydrophilicity, thus decreasing microbial adhesion and biofilm formation [75]. Such modifications have a temporary effect on silicone, due to the rapid rearrangement of macromolecular chains, leading to surface hydrophobicity recovery [76]. Surface coatings releasing biocides (e.g. nitric oxide, antibiotics or silver) have been developed on metallic and polymer biomaterials, as short term antimicrobial strategies [77]. The main drawbacks of antimicrobial coatings arise from time limited effectiveness as in the case of PEG-based coatings, which are susceptible to oxidative degradation [78], development of microorganism resistance and potential toxicity towards human cells as in the case of quaternary ammonium salts coatings [79].

    In this context, biosurfactants have recently emerged as a potential new generation of anti-adhesive agents with enhanced biocompatibility. Biosurfactants have demonstrated the ability to disrupt biofilm formation, controlling microbial interaction with interfaces by altering the chemical and physical condition of the developing biofilms environments [30,80].

    2.3.1. Anti-adhesives/biofilm lipopeptides biosurfactants

    Rivardo et al. [81], reported that a lipopeptide biosurfactant produced by the strain B. subtilis V9T14 in association with antibiotics synergistically increased the efficacy of antibiotics against biofilm formation of the pathogenic E. coli CFT073. Some of the combinations used led to the complete eradication of its biofilm. This has been used to obtain an international patent on this application [82]. Combinations of the biosurfactant with biocides to act as adjuvants were designed to effectively prevent biofilms formation on biotic and abiotic surfaces and/or eradicating planktonic bacterial growth.

    Janek et al. [83] investigated the role and applications of pseudofactin II, cyclic lipopeptide biosurfactant produced by Pseudomonas fluorescens BD5, as an anti-adhesive compound for therapeutic and medicinal applications. Pseudofactin II decreased the adhesion of Enterococcus hirae, Proteus mirabilis, S. epidermidis, E. faecalis, E. coli, and C. albicans to glass, polystyrene and silicone. In particular, pre-treatment of a polystyrene surface with pseudofactin II (0.5 mg ml−1) reduced C. albicans adhesion by 92-99% and other bacterial adhesion by 36-90%. It also led to increased biofilm removal ability on pre-existing biofilms grown on untreated surfaces. Pseudofactin II also caused a significant inhibition of the initial adhesion of E. coli, E. hirae, E. faecalis and C. albicans strains onto silicone urethral catheters. At the highest concentration tested (0.5 mg ml−1) total inhibition of growth was observed for S. epidermidis while partial growth inhibitions occurred on other bacteria and C. albicans yeast.

    In other work, Paenibacillus polymyxa lipopeptide biosurfactants were able to inhibit mixed and single species biofilms [84]. This biosurfactant complex mainly composed of fusaricidin B and polymyxin D1, reduced the biofilm biomass for P. aeruginosa, S. aureus, B. subtilis, Micrococcus luteus, and Streptococcus bovis. Sriram et al. [85] also reported antimicrobial activity and biofilm inhibition using a lipopeptide biosurfactant produced by a soil strain of Bacillus cereus resistant to the heavy metals lead, iron and zinc. It also inhibited biofilm formation in pathogenic strains of S. aureus and P. aeruginosa. Maximum biofilm inhibition (57%) was observed against S. epidermidis at 15 mg ml−1.

    Zeraik and Nitschke [86] evaluated the anti-adhesive and attachment properties for M. luteus, L. monocytogenes and S. aureus on polystyrene surfaces at various temperatures upon treatment with rhamnolipids and surfactin. Rhamnolipids showed a slight decrease in the attachment of S. aureus but were generally not effective. Surfactin in comparison effectively inhibited adhesion of tested bacterial strains at all conditions with increased activity as temperature decreased with maximum 63-66% reduction in adhesion at 4 °C.

    Prevention of C. albicans biofilm formation on silicone disks and on acrylic resins for denture prostheses by lipopeptide biosurfactants produced by Bacillus sp. were reported by Cochis et al. [12]. Pre-coating with biosurfactants resulted in greater biofilm reduction and drop in cell number viability than did chlorhexidine disinfectant. This anti-adhesion activity was detected at fairly low concentrations (78-156 μg ml−1) which were non-cytotoxic. In another work, the lipopeptide biosurfactant produced by Bacillus tequilensis CH (CHBS) was able to inhibit biofilm formation of pathogenic bacteria on both hydrophilic and hydrophobic surfaces [87]. E. coli and S. mutans biofilms were grown with different concentrations of biosurfactant on glass pieces or polyvinyl chloride surfaces. Biofilms of E. coli and S. mutans were observed on the surfaces co-incubated with 0 and 25 μg ml−1 CHBS, whereas there was a complete absence of biofilm on the surfaces incubated with 50 and 75 μg ml−1 CHBS. Interestingly, CHBS did not inhibit the growth of E. coli and S. mutans planktonic cells under all tested concentrations, demonstrating that CHBS was not a bactericidal agent but only contrasted bacterial adhesion to different surfaces [87].

    Recent research at the author’s laboratory reported on a biosurfactant produced by Lactobacillus brevis CV8LAC, which significantly reduced biofilm formation and adhesion of C. albicans on silicone elastomeric disks [88]. In particular, co-incubation with CV8LAC biosurfactant significantly reduced biofilm formation by about 90%, whereas pre-coating of silicone disks reduced fungal adhesion of about 60%. The growth of C. albicans in both sessile and planktonic form was not inhibited; suggesting that biosurfactant CV8LAC remarkably affected cell-surface interactions making the surface less supportive for microbial adhesion.

    Recent unpublished results from our laboratory also showed a significant reduction of biofilm formation by bacterial pathogens on polystyrene coated with a lipopeptide biosurfactant obtained from an endophytic strain, genotypically identified as Bacillus subtilis (Figure 1A). In particular, biofilms of three P. aeruginosa strains were reduced in a range of about 70-90%, whereas biofilms of E. coli and S. epidermidis strains were inhibited of about 70%.

    Figure 1. Anti-adhesion activity of a lipopeptide biosurfactant (LpBS) against bacterial and fungal biofilm. (A) Bacterial biofilm reduction on polystyrene coated by a LpBS after 24 hours incubation. The assay was carried out in Calgary Biofilm Device [MBEC Minimum Biofilm Eradication Concentration] AssayTM, Innovotech, St. Edmonton, Canada] by means of MTT method. The asterisks [*] indicate the level of statistical significance as determined by Student’s t-test [*** p < 0.001]; (B) C. albicans biofilm reduction on silicone disks coated with a lipopeptide biosurfactant at 24, 48 and 72 hours of incubation.

    The same lipopeptide also showed the ability to significantly reduce biofilm formation for C. albicans on biosurfactant-coated silicone elastomeric disks (Figure 1B). Chemical analysis of the crude extract revealed the presence of two families of lipopeptides, principally surfactin and a lower percentage of fengycin.

    2.3.2. Anti-adhesives/biofilm glycolipid biosurfactants

    Rhamnolipids and other surface-active plant oil extracts have recently been observed by some of the author’s laboratories to have a significant role in the inhibition of complex biofilms and to act as adjuvants enhancing selected antibiotics microbial inhibitors [15]. In another study, a glycolipid biosurfactant from P. aeruginosa DSVP20 was evaluated for its ability to disrupt C. albicans biofilm. The treatment with the di-rhamnolipid (RL-2) at concentrations ranging from 0.04-5.0 mg ml−1 significantly reduced C. albicans adhesion on polystyrene surfaces (PS) in a dose-dependent manner. Data showed a reduction of the number of adherent cells, after 2h of treatment, of about 50% with 0.16 mg ml−1 RL-2, that gradually increased up to a complete inhibition of adherence at a concentration of 5 mg ml−1. Moreover, C. albicans biofilm on PS surface was disrupted up to 70% and 90% with RL-2 treatment at concentrations of 2.5 and 5.0 mg ml−1, respectively [89]. Also recently, Pradhan et al. [90] reported a new glycolipid obtained from Lysinibacillus fusiformis S9 with remarkable anti-biofilm activity against pathogenic E. coli and S. mutans, while not affecting microbial cell viability. In particular, the biosurfactant was able to completely contain the biofilms formation at a concentration of 40 μg ml−1.

    Recent unpublished data obtained at the author’s laboratory investigating anti-biofilm activities of rhamnolipid biosurfactants against Gram-negative and Gram-positive pathogens on polystyrene are presented in Figure 2. The rhamnolipid extract, obtained from a P. aeruginosa isolated from cystic fibrosis patient (strain 89), was utilized at a concentration of 500 μg ml−1.

    Figure 2. Bacterial biofilm reduction on polystyrene coated by a rhamnolipid biosurfactant (RhBS) after 24 hours incubation. The assay was carried out in Calgary Biofilm Device [MBEC Minimum Biofilm Eradication Concentration] AssayTM, Innovotech, St. Edmonton, Canada] by means of MTT method. The asterisks [*] indicate the level of statistical significance as determined by Student’s t-test [* p < 0.05; ** p < 0.01].

    It was observed that rhamnolipid significantly reduced biofilm formation abilities of the Gram-positive S. epidermidis and the Gram-negative E. coli respectively of 75% and 82%. The observed reductions of three related P. aeruginosa strains were at average of 31%. It is known that rhamnolipids play an important role at different stages of P. aeruginosa biofilm development and that their effect is concentration-dependent. While low amounts of rhamnolipids increase initial adherence of cells to a surface and microcolonies formation, the presence of high concentrations in the medium (as in the case of the anti-adhesion assay), limits attachment of the cells and further microcolonies formation [91], most likely leading to a reduction of biofilm.

    Additional chemical analyses are underway to identify the type rhamnolipids produced by P. aeruginosa 89 strain, however it most likely will be a mixture of the mono and di rhamnolipids with the 10 carbon fatty acids side chains typical of P. aeruginosa strain. The encouraging results obtained against biofilm producer strains make this biosurfactant a good candidate to prevent adhesion on plastic surfaces.

    Padmapriya and Suganthi [92] have partially purified two biosurfactant produced by C. tropicalis and C. albicans and tested their anti-adhesive activity on different types of urinary and clinical pathogens. The results showed a reduction of adherent cells on the surface of urinary catheter pre-coated with biosurfactants and a higher activity of the biosurfactant synthesized by C. tropicalis in comparison with the biosurfactant synthesized by C. albicans.

    The effect of the Lactobacillus acidophilus DSM 20079 biosurfactant on adherence and on the expression level of the genes gtf B and gtf C in S. mutans biofilm cells was also analyzed by Tahmourespour et al. [93]. The L. acidophilus biosurfactant was able to interfere with the adhesion and biofilm formation of S. mutans to glass slide and led to shorter chains formation. Moreover, several properties of S. mutans cells (adhesion ability, biofilm formation, surface properties and gene expression) were altered as a result of treatment with L. acidophilus biosurfactant. A patent has been granted for Lactobacillus biosurfactants ability to inhibit bacterial pathogens attachment and colonization on medical devices particularly to prevent urogenital infection in mammals [94]. The anti-adhesive activity of a lipopeptide biosurfactant secreted by the probiotic strain Propionibacterium freudenreichii was analysed by Hajfarajollah et al. [95]. It showed a significant anti-adhesive action against a wide range of pathogenic bacteria and fungi (S. aureus, B. cereus, P. aeruginosa, E. coli). The highest adhesion reduction was obtained for P. aeruginosa (67.1%) at the concentration of 40 mg ml-1, whereas lower activities were observed for S. aureus (32.3%), B. cereus (39.1 %) and E. coli (47.7%), at the same concentration.

    2.4. Therapeutic and biotechnological applications

    Mannosylerythritol lipids (MELs), surfactin and trehalose lipids, all often reported as very powerful biosurfactant molecules, are known to have immunosuppressive and immunomodulating, anti-tumour and anti-inflammatory activity in addition to other properties such as cells stimulation and differentiation, cell-to-cell signalling, self-assembling, interaction with stratum corneum lipids, membrane perturbation and haemolytic activity [6]. Antitumor activities were described for surfactin by Cao et al. [96] and for other lipopeptides by Saini et al. [35]. Significant effects against tumour cell lines were also observed for serratamolide AT514, a cyclic depsipeptide from Serratia marcescens [97] and for glycolipids, in particular mannosylerythritol lipids (MELs) [98] and sophorolipid [99].

    Surfactin also showed interesting anti-inflammatory activities due to its inhibitory properties onphospholipase A2, on the release of Interleukin (LK-6) and the overproduction of nitric oxide [100]. Park et al. [101] explored the mechanisms by which surfactin induced anti-inflammatory actions in relation to serious gum infection caused by Porphyromonas gingivalis. These authors also observed that surfactin significantly reduced the pro-inflammatory cytokines, including interleukin IL-6, IL-12 and IL-1β andtumour necrosis factor-α, through suppression of nuclear factor κB activity in P. gingivalis. The role of surfactin in the inhibition of the immunostimulatory functions of macrophages through blocking the NK-κB (nuclear factor kappa-light-chain-enhancer of activated B cells) and MAPK (mitog en-activated protein kinases) and Akt (serine/threonine kinase Akt, also known as protein kinase) cell signalling pathway suggests important immunosuppressive capabilities for this molecule [102].

    Properties such as emulsification, foaming, detergency, and dispersion render biosurfactants curious molecules with several potential application in areas of drug delivery [103]. Rhamnolipids liposomes have been patented some time ago as drug and other molecules delivery system as microcapsules containing these drugs, proteins, nucleic acids and dyes and with an the ability to biomimetic biological membranes and acting as sensors for the detection of pH variations. Nguyen et al. [104] reported on using sophorolipids and rhamnolipids mixed with lecithins to prepare biocompatible micro-emulsions suitable for both cosmetic and drug delivery applications. Other biosurfactants such as fengycin and surfactin were also reported suitable as enhancers for the skin accumulation and transdermal penetration of antiviral drug acyclovir increasing its concentration in the epidermis by a factor of two [105].

    Finally, biosurfactant mediated nanomaterial synthesis and/or stabilization has recently been emerging as a “green chemistry” clean, non-toxic and environmentally acceptable procedure [106]. Reddy et al. [107] successfully synthesized gold and silver nanoparticles by using surfactin from the bacterium B. subtilis while Singh et al. [108] synthesized a highly stable cadmium sulphide nanoparticles using surfactin from B. amyloliquefaciens KSU-109 and both sophorolipids and rhamnolipids were successfully used in the synthesis and stabilization of metal-bound nanoparticles. Palanisamy and Raichur [109] and Kumar et al. [110] synthesized spherical nickel oxide and silver nanoparticles using rhamnolipids as alternative surfactant through microemulsion technique and reported antimicrobial activity with the silver nanoparticles. Sophorolipids were also successfully used to attach silver nanoparticles to polymer scaffolding and passing on antibacterial activity [111].

    3. Conclusions

    Microbial biofilms are recalcitrant environments often providing shelter and protection to producing and inhabiting microbial flora. They also are mainly responsible for many persistent infections in clinical environments, the dissemination of airborne pathogens and the fouling of industrial surfaces in clinical, food and environmental settings. These problems are progressively challenged by the increase in resistant microbial biofilm populations and the scarcity of alternative eradication solutions. Biosurfactants represent a group of emerging surface-active agents which have inherent anti-microbial (bacterial, fungal and viral) properties and ability to act as anti-adhesive, disruptive and dispersant for such biofilm structures. Their uses either on their own or as adjuvants to other antimicrobial, chemotherapies may represent a possible way forward in tackling infections, biofilms formation and microbial proliferation in the future.

    Conflict of Interest

    The authors report no conflicts of interest.

    [1] Megharaj M, Ramakrishnan B, Venkateswarlu K, et al. (2011) Bioremediation approaches for organic pollutants: A critical perspective. Environ Int 37: 1362–1375. doi: 10.1016/j.envint.2011.06.003
    [2] Bezza FA, Nkhalambayausi Chirwa EM (2016) Biosurfactant-enhanced bioremediation of aged polycyclic aromatic hydrocarbons (PAHs) in creosote contaminated soil. Chemosphere 144: 635–644. doi: 10.1016/j.chemosphere.2015.08.027
    [3] de la Cueva SC, Rodríguez CH, Cruz NOS, et al. (2016) Changes in Bacterial Populations During Bioremediation of Soil Contaminated with Petroleum Hydrocarbons. Water Air Soil Poll 227: 1–12. doi: 10.1007/s11270-015-2689-7
    [4] Dadrasnia A, Shahsavari N, Salmah I (2015) The top 101 cited articles in environmental clean-up: Oil spill remediation. Global NEST J 17: 692–700.
    [5] Chirwa E, Smit H (2010) Simultaneous Cr (VI) reduction and phenol degradation in a trickle bed bioreactor: shock loading response. Chem Eng Trans 20: 55–60.
    [6] Dixit R, Malaviya D, Pandiyan K, et al. (2015) Bioremediation of heavy metals from soil and aquatic environment: an overview of principles and criteria of fundamental processes. Sustainability 7: 2189–2212. doi: 10.3390/su7022189
    [7] Bustamante M, Durán N, Diez M (2012) Biosurfactants are useful tools for the bioremediation of contaminated soil: a review. J Soil Sci Plant Nut 12: 667–687.
    [8] Abdel-Moghny T, Mohamed RS, El-Sayed E, et al. (2012) Removing of hydrocarbon contaminated soil via air flushing enhanced by surfactant. Appl Petrochem Res 2: 51–59. doi: 10.1007/s13203-012-0008-4
    [9] Dadrasnia A, Ismail S (2015) Biosurfactant Production by Bacillus salmalaya for Lubricating Oil Solubilization and Biodegradation. Int J Environ Res Pub Heal 12: 9848. doi: 10.3390/ijerph120809848
    [10] Santos DK, Brandão YB, Rufino RD, et al. (2014) Optimization of cultural conditions for biosurfactant production from Candida lipolytica. Biocat Agr Biotechnol 3: 48–57.
    [11] Dyke MIV, Couture P, Brauer M, et al. (1993) Pseudomonas aeruginosa UG2 rhamnolipid biosurfactants: structural characterization and their use in removing hydrophobic compounds from soil. Can J Microbiol 39: 1071–1078. doi: 10.1139/m93-162
    [12] Lima ÁS, Alegre RM (2009) Evaluation of emulsifier stability of biosurfactant produced by Saccharomyces lipolytica CCT-0913. Braz Arch Biol Tech 52: 285–290. doi: 10.1590/S1516-89132009000200004
    [13] Nwachi AC, Onochie CC, Iroha IR, et al. (2016) Extraction of Biosurfactants Produced from Bacteria Isolated from Waste-Oil Contaminated Soil in Abakaliki Metropolis, Ebonyi State. J Biotechnol Res 2: 24–30.
    [14] Walter V, Syldatk C, Hausmann R (2013) Screening Concepts for the Isolation of Biosurfactant Producing Microorganisms. In: Madame Curie Bioscience Database [Internet]. Austin (TX): Landes Bioscience; 2000–2013.
    [15] Cortés-Sánchez AdJ, Hernández-Sánchez H, Jaramillo-Flores ME (2013) Biological activity of glycolipids produced by microorganisms: New trends and possible therapeutic alternatives. Microbiol Res 168: 22–32. doi: 10.1016/j.micres.2012.07.002
    [16] Vijayakumar S, Saravanan V (2015) Biosurfactants-Types, Sources and Applications. Res J Microbiol 10: 181. doi: 10.3923/jm.2015.181.192
    [17] Neu TR (1996) Significance of bacterial surface-active compounds in interaction of bacteria with interfaces. Microbiol Rev 60: 151–166.
    [18] Rosenberg E, Ron E (1999) High-and low-molecular-mass microbial surfactants. Appl Microbiol Biotechnol 52: 154–162. doi: 10.1007/s002530051502
    [19] Perfumo A, Smyth TJP, Marchant R, et al. (2010) Production and roles of biosurfactants and bioemulsifiers in accessing hydrophobic substrates. In: Kenneth N. Timmis (ed.), Handbook of Hydrocarbon and Lipid Microbiology, Springer. UK. 2, 1501–1512.
    [20] Mulligan CN, Gibbs BF (1990) Recovery of biosurfactants by ultrafiltration. J Chem Technol Biotechnol 47: 23–29.
    [21] Whang L-M, Liu P-WG, Ma C-C, et al. (2008) Application of biosurfactants, rhamnolipid, and surfactin, for enhanced biodegradation of diesel-contaminated water and soil. J Hazard Mater 151: 155–163. doi: 10.1016/j.jhazmat.2007.05.063
    [22] Souza EC, Vessoni-Penna TC, de Souza Oliveira RP (2014) Biosurfactant-enhanced hydrocarbon bioremediation: An overview. Int Biodeter Biodeg 89: 88–94. doi: 10.1016/j.ibiod.2014.01.007
    [23] Uzoigwe C, Burgess JG, Ennis CJ, et al. (2015) Bioemulsifiers are not biosurfactants and require different screening approaches. Front Microbiol 6: 245.
    [24] Dadrasnia A, Usman MM, Wei KSC, et al. (2016) Native soil bacterial isolate in Malaysia exhibit promising supplements on degrading organic pollutants. Process Saf Environ 100: 264–271.
    [25] Rahman PK, Gakpe E (2008) Production, characterisation and applications of biosurfactants-Review. Biotech 7: 360–370 doi: 10.3923/biotech.2008.360.370
    [26] Karanth N, Deo P, Veenanadig N (1999) Microbial production of biosurfactants and their importance. Curr Sci 77: 116–126.
    [27] Sáenz-Marta CI, de Lourdes Ballinas-Casarrubias M, Rivera-Chavira BE, et al. (2015) Biosurfactants as Useful Tools in Bioremediation. Advances in Bioremediation of Wastewater and Polluted Soil: INTECH. DOI: 10.5772/60751
    [28] Muthusamy K, Gopalakrishnan S, Ravi TK, et al. (2008) Biosurfactants: Properties, commercial production and application. Curr Sci (00113891) 94.
    [29] Gautam K, Tyagi V (2006) Microbial surfactants: a review. J Oleo Sci 55: 155–166. doi: 10.5650/jos.55.155
    [30] Rosenberg E, Ron EZ (2013) Biosurfactants. The Prokaryotes: Springer. pp. 281–294.
    [31] Silva RdCFS, Almeida DG, Rufino RD, et al. (2014) Applications of Biosurfactants in the Petroleum Industry and the Remediation of Oil Spills. Int J Mol Sci 15: 12523–12542. doi: 10.3390/ijms150712523
    [32] Kosaric N (2008) Biosurfactants. Biotechnology Set: Wiley-VCH Verlag GmbH. pp. 659–717.
    [33] Cooper DG, Paddock DA (1983) Torulopsis petrophilum and Surface Activity. Appl Environ Microbiol 46: 1426–1429.
    [34] Thavasi R, Subramanyam Nambaru VRM, Jayalakshmi S, et al. (2011) Biosurfactant Production by Pseudomonas aeruginosa from Renewable Resources. Indian J Microbiol 51: 30–36. doi: 10.1007/s12088-011-0076-7
    [35] Duvnjak Z, Cooper DG, Kosaric N (1982) Production of surfactant by Arthrobacter paraffineus ATCC 19558. Biotechnol Bioeng 24: 165–175. doi: 10.1002/bit.260240114
    [36] Müller MM, Hörmann B, Kugel M, et al. (2011) Evaluation of rhamnolipid production capacity of Pseudomonas aeruginosa PAO1 in comparison to the rhamnolipid over-producer strains DSM 7108 and DSM 2874. Appl Microbiol Biotechnol 89: 585–592. doi: 10.1007/s00253-010-2901-z
    [37] Hewald S, Josephs K, Bölker M (2005) Genetic analysis of biosurfactant production in Ustilago maydis. Appl Environ Microbiol 71: 3033–3040. doi: 10.1128/AEM.71.6.3033-3040.2005
    [38] Cooper DG, Paddock DA (1984) Production of a Biosurfactant from Torulopsis bombicola. Appl Environ Microbiol 47: 173–176.
    [39] Matvyeyeva OL, Vasylchenko, Aliiev§Ñ OR (2014) Microbial Biosurfactants Role in Oil Products Biodegradation. Int J Environ Bioremediat Biodegradat 2: 69–74.
    [40] Pacwa-Płociniczak M, Płaza GA, Piotrowska-Seget Z, et al. (2011) Environmental Applications of Biosurfactants: Recent Advances. Intl J Mol Sci 12: 633–654. doi: 10.3390/ijms12010633
    [41] Cameotra SS, Singh P (2009) Synthesis of rhamnolipid biosurfactant and mode of hexadecane uptake by Pseudomonas species. Microb Cell Fact 8: 1–7. doi: 10.1186/1475-2859-8-1
    [42] Berg G, Seech AG, Lee H, et al. (1990) Identification and characterization of a soil bacterium with extracellular emulsifying activity. J Environ Sci Heal A 25: 753–764.
    [43] Sen R (2010) Biosurfactants: Springer New York. 331 p.
    [44] Franzetti A, Gandolfi I, Bestetti G, et al. (2010) Production and applications of trehalose lipid biosurfactants. Eur J Lipid Sci Tech 112: 617–627. doi: 10.1002/ejlt.200900162
    [45] Chen Y-G, Wang Y-X, Zhang Y-Q, et al. (2009) Nocardiopsis litoralis sp. nov., a halophilic marine actinomycete isolated from a sea anemone. Int J Syst Evol Microbiol 59: 2708–2713.
    [46] Bennur T, Kumar AR, Zinjarde S, et al. (2015) Nocardiopsis species: Incidence, ecological roles and adaptations. Microbiol Res 174: 33–47. doi: 10.1016/j.micres.2015.03.010
    [47] Jennema GE, McInerney MJ, Knapp R, M., , et al. (1983) A halotolerant, biosurfactants-producing Bacillus species potentially useful for enhanced oil recovery. Dev Ind Microbiol 24: 485–492.
    [48] Cirigliano MC, Carman GM (1985) Purification and Characterization of Liposan, a Bioemulsifier from Candida lipolytica. Appl Environ Microbiol 50: 846–850.
    [49] Cameron DR, Cooper DG, Neufeld RJ (1988) The mannoprotein of Saccharomyces cerevisiae is an effective bioemulsifier. Appl Environ Microbiol 54: 1420–1425.
    [50] Appanna VD, Finn H, St Pierre M (1995) Exocellular phosphatidylethanolamine production and multiple-metal tolerance in Pseudomonas fluorescens. FEMS Microbiol Lett 131: 53–56. doi: 10.1111/j.1574-6968.1995.tb07753.x
    [51] Silva RdCFS, Almeida DG, Rufino RD, et al. (2014) Applications of Biosurfactants in the Petroleum Industry and the Remediation of Oil Spills. Intl J Mol Sci 15: 12523–12542. doi: 10.3390/ijms150712523
    [52] Reis RS, Pacheco GJ, Pereira AG, et al. (2013) Biosurfactants: Production and Applications, Biodegradation - Life of Science, Dr. Rolando Chamy (Ed.), InTech, DOI: 10.5772/56144.
    [53] Banat IM, Satpute SK, Cameotra SS, et al. (2014) Cost effective technologies and renewable substrates for biosurfactants’ production. Front Microbiol 5: 697.
    [54] Banat IM (1995) Biosurfactants production and possible uses in microbial enhanced oil recovery and oil pollution remediation: A review. Bioresource Technol 51: 1–12. doi: 10.1016/0960-8524(94)00101-6
    [55] Banat IM (1995) Characterization of biosurfactants and their use in pollution removal – State of the Art. (Review). Acta Biotechnologica 15: 251–267. doi: 10.1002/abio.370150302
    [56] McInerney MJ, Han SO, Maudgalya S, et al. (2003) Development of More Effective Biosurfactants for Enhanced Oil Recovery. U.S. Department of Energy, Tulsa, Oklahoma. DOE/BC/15113e2. Available online at: http://www.netl.doe.gov/KMD/cds/disk44/I-Microbial/BC15113_2.pdf (accessed 28.06.2016.).
    [57] Bachmann RT, Johnson AC, Edyvean RGJ (2014) Biotechnology in the petroleum industry: An overview. Int Biodeter Biodeg 86, Part C: 225–237.
    [58] Mulligan CN (2005) Environmental applications for biosurfactants. Environmental Pollution 133: 183–198. doi: 10.1016/j.envpol.2004.06.009
    [59] Cameotra S, Makkar R (1998) Synthesis of biosurfactants in extreme conditions. Appl Microbiol Biotechnol 50: 520–529. doi: 10.1007/s002530051329
    [60] Rodrigues L, Banat IM, Teixeira J, et al. (2006) Biosurfactants: potential applications in medicine. J Antimicrob Chemoth 57: 609–618.
    [61] Poremba K, Gunkel W, Lang S, et al. (1991) Marine biosurfactants, III. Toxicity testing with marine microorganisms and comparison with synthetic surfactants. Zeitschrift für Naturforschung C 46: 210–216.
    [62] Lima TMS, Procópio LC, Brandão FD, et al. (2011) Evaluation of bacterial surfactant toxicity towards petroleum degrading microorganisms. Bioresource Technol 102: 2957–2964. doi: 10.1016/j.biortech.2010.09.109
    [63] Gudiña EJ, Rodrigues AI, Teixeira JA, et al. (2015) New microbial surface-active compounds: the ultimate alternative to chemical surfactants? SINAFERM 2015 XX Simpósio Nacional de Bioprocessos. Fortaleza, Brazil, Sep. 1-4, 1-5, 2015.
    [64] Korcan SE, Ciğerci İH, Konuk M (2013) White-Rot Fungi in Bioremediation. Fungi as Bioremediators: Springer. pp. 371–390.
    [65] Barr DP, Aust SD (1994) Mechanisms white rot fungi use to degrade pollutants. Environ Sci Technol 28: 78A–87A. doi: 10.1021/es00051a724
    [66] Parmar B, Mervana P, Vyas B (2015) Degradation of textile dyes by white rot basidiomycetes. Lifesci Leaflet 59: 62–75.
    [67] Cookson J (1995) Bioremediation engineering: design and application: McGraw Hill, New York.
    [68] Peng X, Yuan X-z, Liu H, et al. (2015) Degradation of Polycyclic Aromatic Hydrocarbons (PAHs) by Laccase in Rhamnolipid Reversed Micellar System. Appl Biochem Biotechnol 176: 45–55. doi: 10.1007/s12010-015-1508-3
    [69] Liu Z-F, Zeng G-M, Zhong H, et al. (2012) Effect of dirhamnolipid on the removal of phenol catalyzed by laccase in aqueous solution. World J Microbiol Biot 28: 175–181. doi: 10.1007/s11274-011-0806-3
    [70] Shin K-H, Kim K-W, Ahn Y (2006) Use of biosurfactant to remediate phenanthrene-contaminated soil by the combined solubilization–biodegradation process. J Hazard Mater 137: 1831–1837. doi: 10.1016/j.jhazmat.2006.05.025
    [71] Santos D, Rufino R, Luna J, et al. (2016) Biosurfactants: Multifunctional Biomolecules of the 21st Century. Intl J Mol Sci 17: 401. doi: 10.3390/ijms17030401
    [72] Wuana RA, Okieimen FE (2011) Heavy Metals in Contaminated Soils: A Review of Sources, Chemistry, Risks and Best Available Strategies for Remediation. ISRN Ecology 2011: 20.
    [73] Miller RM (1995) Biosurfactant-facilitated remediation of metal-contaminated soils. Environ Health Persp 103: 59–62. doi: 10.1289/ehp.95103s459
    [74] Babich H, Stotzky G (1983) Temperature, pH, salinity, hardness, and particulates mediate nickel toxicity to eubacteria, an actinomycete, and yeasts in lake, simulated estuarine, and sea waters. Aquat Toxicol 3: 195–208. doi: 10.1016/0166-445X(83)90040-1
    [75] Babich H, Stotzky G (1982) Nickel toxicity to microbes: Influence of pH and implications for acid rain. Environ Res 29: 335–350. doi: 10.1016/0013-9351(82)90035-4
    [76] Sandrin TR, Maier RM (2002) Effect of pH on cadmium toxicity, speciation, and accumulation during naphthalene biodegradation. Environ Toxicol Chem 21: 2075–2079. doi: 10.1002/etc.5620211010
    [77] Olaniran AO, Balgobind A, Pillay B (2013) Bioavailability of Heavy Metals in Soil: Impact on Microbial Biodegradation of Organic Compounds and Possible Improvement Strategies. Intl J Mol Sci 14: 10197–10228. doi: 10.3390/ijms140510197
    [78] Batista S, Mounteer A, Amorim F, et al. (2006) Isolation and characterization of biosurfactant/bioemulsifier-producing bacteria from petroleum contaminated sites. Bioresource Technol 97: 868–875. doi: 10.1016/j.biortech.2005.04.020
    [79] Liu B, Liu J, Ju M, et al. (2016) Purification and characterization of biosurfactant produced by Bacillus licheniformis Y-1 and its application in remediation of petroleum contaminated soil. Mar Pollut Bull 107: 46–51. doi: 10.1016/j.marpolbul.2016.04.025
    [80] Yan P, Lu M, Yang Q, et al. (2012) Oil recovery from refinery oily sludge using a rhamnolipid biosurfactant-producing Pseudomonas. Bioresource Technol 116: 24–28. doi: 10.1016/j.biortech.2012.04.024
    [81] Liu W, Wang X, Wu L, et al. (2012) Isolation, identification and characterization of Bacillus amyloliquefaciens BZ-6, a bacterial isolate for enhancing oil recovery from oily sludge. Chemosphere 87: 1105–1110. doi: 10.1016/j.chemosphere.2012.01.059
    [82] Zhou W, Wang X, Chen C, et al. (2013) Enhanced soil washing of phenanthrene by a plant-derived natural biosurfactant, Sapindus saponin. Colloid Surface A 425: 122–128. doi: 10.1016/j.colsurfa.2013.02.055
    [83] Lau EV, Gan S, Ng HK, et al. (2014) Extraction agents for the removal of polycyclic aromatic hydrocarbons (PAHs) from soil in soil washing technologies. Environ Poll 184: 640–649. doi: 10.1016/j.envpol.2013.09.010
    [84] Deziel E, Paquette G, Villemur R, et al. (1996) Biosurfactant production by a soil pseudomonas strain growing on polycyclic aromatic hydrocarbons. Appl Environ Microbiol 62: 1908–1912.
    [85] Mao X, Jiang R, Xiao W, et al. (2015) Use of surfactants for the remediation of contaminated soils: A review. J Hazard Mater 285: 419–435. doi: 10.1016/j.jhazmat.2014.12.009
    [86] Ye M, Sun M, Wan J, et al. (2015) Evaluation of enhanced soil washing process with tea saponin in a peanut oil–water solvent system for the extraction of PBDEs/PCBs/PAHs and heavy metals from an electronic waste site followed by vetiver grass phytoremediation. J Chem Technol Biot 90: 2027–2035. doi: 10.1002/jctb.4512
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    7. Lakshmi Tripathi, Matthew S. Twigg, Aikaterini Zompra, Karina Salek, Victor U. Irorere, Tony Gutierrez, Georgios A. Spyroulias, Roger Marchant, Ibrahim M. Banat, Biosynthesis of rhamnolipid by a Marinobacter species expands the paradigm of biosurfactant synthesis to a new genus of the marine microflora, 2019, 18, 1475-2859, 10.1186/s12934-019-1216-8
    8. Breedge Callaghan, Helen Lydon, Sophie L. K. W. Roelants, Inge N. A. Van Bogaert, Roger Marchant, Ibrahim M. Banat, Christopher A. Mitchell, Chunming Liu, Lactonic Sophorolipids Increase Tumor Burden in Apcmin+/- Mice, 2016, 11, 1932-6203, e0156845, 10.1371/journal.pone.0156845
    9. Ibrahim M. Banat, Quentin Carboué, Gerardo Saucedo-Castañeda, José de Jesús Cázares-Marinero, Biosurfactants: The green generation of speciality chemicals and potential production using Solid-State fermentation (SSF) technology, 2021, 320, 09608524, 124222, 10.1016/j.biortech.2020.124222
    10. Sonam Gupta, Navdeep Raghuwanshi, Ritu Varshney, I.M. Banat, Amit Kumar Srivastava, Parul A. Pruthi, Vikas Pruthi, Accelerated in vivo wound healing evaluation of microbial glycolipid containing ointment as a transdermal substitute, 2017, 94, 07533322, 1186, 10.1016/j.biopha.2017.08.010
    11. T. P. Pirog, SYNERGISTIC ACTION OF ESSENTIAL OILS WITH THE BIOCIDES ON MICROORGANISMS, 2019, 12, 24107751, 5, 10.15407/biotech12.04.005
    12. Rachhpal S. Kahlon, 2016, Chapter 8, 978-3-319-31197-5, 281, 10.1007/978-3-319-31198-2_8
    13. Yuan-Seng Wu, Siew-Ching Ngai, Bey-Hing Goh, Kok-Gan Chan, Learn-Han Lee, Lay-Hong Chuah, Anticancer Activities of Surfactin and Potential Application of Nanotechnology Assisted Surfactin Delivery, 2017, 8, 1663-9812, 10.3389/fphar.2017.00761
    14. B. Jasim, S. Sreelakshmi, Jyothis Mathew, E. K. Radhakrishnan, Identification of endophytic Bacillus mojavensis with highly specialized broad spectrum antibacterial activity, 2016, 6, 2190-572X, 10.1007/s13205-016-0508-5
    15. C. Ceresa, L. Fracchia, M. Williams, I.M. Banat, M.A. Díaz De Rienzo, The effect of sophorolipids against microbial biofilms on medical-grade silicone, 2020, 309, 01681656, 34, 10.1016/j.jbiotec.2019.12.019
    16. T.P. Pirog, L.V. Nikituk, T.A. Shevchuk, Synergism of Antimicrobial Activity of Nocardia vaccinii IMV B-7405 Surfactants and Antibiotics, 2017, 79, 10280987, 30, 10.15407/microbiolj79.04.030
    17. Chiara Ceresa, Francesco Tessarolo, Devid Maniglio, Erica Tambone, Irene Carmagnola, Emanuele Fedeli, Iole Caola, Giandomenico Nollo, Valeria Chiono, Gianna Allegrone, Maurizio Rinaldi, Letizia Fracchia, Medical-Grade Silicone Coated with Rhamnolipid R89 Is Effective against Staphylococcus spp. Biofilms, 2019, 24, 1420-3049, 3843, 10.3390/molecules24213843
    18. Victória Akemi I. Silveira, Erick K Nishio, Christiane A.U.Q. Freitas, Ismael R. Amador, Renata K.T. Kobayashi, Talita Caretta, Fernando Macedo, Maria Antonia P.C. Celligoi, Production and antimicrobial activity of sophorolipid against Clostridium perfringens and Campylobacter jejuni and their additive interaction with lactic acid, 2019, 21, 18788181, 101287, 10.1016/j.bcab.2019.101287
    19. Bac V. G. Nguyen, Toshiki Nagakubo, Masanori Toyofuku, Nobuhiko Nomura, Andrew S. Utada, Synergy between Sophorolipid Biosurfactant and SDS Increases the Efficiency of P. aeruginosa Biofilm Disruption, 2020, 36, 0743-7463, 6411, 10.1021/acs.langmuir.0c00643
    20. Mohamed Elshikh, Roger Marchant, Ibrahim M. Banat, Geertje van Keulen, Biosurfactants: promising bioactive molecules for oral-related health applications, 2016, 363, 1574-6968, fnw213, 10.1093/femsle/fnw213
    21. T.P. Pirog, O.I. Paliichuk, G.O. Iutynska, T.A. Shevchuk, Prospects of Using Microbial Surfactants in Plant Growing, 2018, 80, 10280987, 115, 10.15407/microbiolj80.03.115
    22. Italo José Batista Durval, Ivison Amaro da Silva, Leonie Asfora Sarubbo, 2021, Chapter 1, 978-981-15-6606-6, 1, 10.1007/978-981-15-6607-3_1
    23. P.J. Naughton, R. Marchant, V. Naughton, I.M. Banat, Microbial biosurfactants: current trends and applications in agricultural and biomedical industries, 2019, 127, 1364-5072, 12, 10.1111/jam.14243
    24. Ana Letícia Silva Coelho, Paulo Emílio Feuser, Bruno Augusto Mattar Carciofi, Cristiano José de Andrade, Débora de Oliveira, Mannosylerythritol lipids: antimicrobial and biomedical properties, 2020, 104, 0175-7598, 2297, 10.1007/s00253-020-10354-z
    25. Chiara Ceresa, Maurizio Rinaldi, Francesco Tessarolo, Devid Maniglio, Emanuele Fedeli, Erica Tambone, Patrizio Caciagli, Ibrahim M. Banat, Mayri Alessandra Diaz De Rienzo, Letizia Fracchia, Inhibitory Effects of Lipopeptides and Glycolipids on C. albicans–Staphylococcus spp. Dual-Species Biofilms, 2021, 11, 1664-302X, 10.3389/fmicb.2020.545654
    26. Matthew L. Smith, Stefano Gandolfi, Philippa M. Coshall, Pattanathu K. S. M. Rahman, Biosurfactants: A Covid-19 Perspective, 2020, 11, 1664-302X, 10.3389/fmicb.2020.01341
    27. Constantina Eleni Drakontis, Samiul Amin, Biosurfactants: Formulations, properties, and applications, 2020, 48, 13590294, 77, 10.1016/j.cocis.2020.03.013
    28. Surekha K. Satpute, Gauri R. Kulkarni, Arun G. Banpurkar, Ibrahim M. Banat, Nishigandha S. Mone, Rajendra H. Patil, Swaranjit Singh Cameotra, Biosurfactant/s from Lactobacilli species: Properties, challenges and potential biomedical applications, 2016, 56, 0233111X, 1140, 10.1002/jobm.201600143
    29. Danyelle Santos, Raquel Rufino, Juliana Luna, Valdemir Santos, Leonie Sarubbo, Biosurfactants: Multifunctional Biomolecules of the 21st Century, 2016, 17, 1422-0067, 401, 10.3390/ijms17030401
    30. Benjamin Trudgeon, Markus Dieser, Narayanaganesh Balasubramanian, Mitch Messmer, Christine M. Foreman, Low-Temperature Biosurfactants from Polar Microbes, 2020, 8, 2076-2607, 1183, 10.3390/microorganisms8081183
    31. Zulfiqar Ali Raza, Zafar M. Khalid, Naseer Ahmad, Bushra Tehseen, Statistical Optimisation of Rhamnolipid Production using a Pseudomonas putida Strain Cultivated on Renewable Carbon Sources of Waste Vegetable Oils, 2020, 57, 0932-3414, 13, 10.3139/113.110664
    32. Farazul Haque, Mohd Sajjad Ahmad Khan, Naif AlQurashi, ROS-Mediated Necrosis by Glycolipid Biosurfactants on Lung, Breast, and Skin Melanoma Cells, 2021, 11, 2234-943X, 10.3389/fonc.2021.622470
    33. Amedea Perfumo, Michelle Rudden, Roger Marchant, Ibrahim M. Banat, 2018, Chapter 35, 978-3-319-50540-4, 75, 10.1007/978-3-319-50542-8_35
    34. Milena G. Rikalovic, Natasa S. Avramovic, Ivanka M. Karadzic, 2017, Chapter 6, 978-953-51-3291-2, 10.5772/66687
    35. A. Pele Milagre, Montero-Rodriguez Dayana, Rubio-Ribeaux Daylin, F. Souza Adriana, A. C. Luna Marcos, F. Santiago Michele, F. S. Andrade Rosileide, A. Lima e Silva Thayse, L. C. M. A. Santiago André, M. Campos-Takaki Galba, Development and improved selected markers to biosurfactant and bioemulsifier production by Rhizopus strains isolated from Caatinga soil, 2018, 17, 1684-5315, 150, 10.5897/AJB2017.16230
    36. Daniel Chakhalian, Robert B. Shultz, Catherine E. Miles, Joachim Kohn, Opportunities for biomaterials to address the challenges of COVID ‐19 , 2020, 108, 1549-3296, 1974, 10.1002/jbm.a.37059
    37. Basavaprabhu H. Nataraj, Syed Azmal Ali, Pradip V. Behare, Hariom Yadav, Postbiotics-parabiotics: the new horizons in microbial biotherapy and functional foods, 2020, 19, 1475-2859, 10.1186/s12934-020-01426-w
    38. Usharani Rathinam Krishnaswamy, Lakshmanaperumalsamy P, Achlesh D, 2021, Chapter 4, 978-981-15-8998-0, 81, 10.1007/978-981-15-8999-7_4
    39. Shanying He, Yaoqi Ni, Li Lu, Qiwei Chai, Tao Yu, Zhiqiang Shen, Chunping Yang, Simultaneous degradation of n-hexane and production of biosurfactants by Pseudomonas sp. strain NEE2 isolated from oil-contaminated soils, 2020, 242, 00456535, 125237, 10.1016/j.chemosphere.2019.125237
    40. T. P. Pirog, THE PROPERTIES OF SURFACTANTS SYNTHESIZED BY Acinetobacter calcoaceticus ІMV В-7241 ON REFINED AND WASTE SUNFLOWER OIL, 2018, 11, 24107751, 82, 10.15407/biotech11.06.082
    41. M. Elshikh, I. Moya-Ramírez, H. Moens, S. Roelants, W. Soetaert, R. Marchant, I.M. Banat, Rhamnolipids and lactonic sophorolipids: natural antimicrobial surfactants for oral hygiene, 2017, 123, 13645072, 1111, 10.1111/jam.13550
    42. Chiara Ceresa, Francesco Tessarolo, Devid Maniglio, Iole Caola, Giandomenico Nollo, Maurizio Rinaldi, Letizia Fracchia, Inhibition of Candida albicans biofilm by lipopeptide AC7 coated medical-grade silicone in combination with farnesol, 2018, 5, 2375-1495, 192, 10.3934/bioeng.2018.3.192
    43. Sweeta Akbari, Nour Hamid Abdurahman, Rosli Mohd Yunus, Fahim Fayaz, Oluwaseun Ruth Alara, Biosurfactants—a new frontier for social and environmental safety: a mini review, 2018, 2, 24520721, 81, 10.1016/j.biori.2018.09.001
    44. Surekha K. Satpute, Nishigandha S. Mone, Parijat Das, Ibrahim M. Banat, Arun G. Banpurkar, Inhibition of pathogenic bacterial biofilms on PDMS based implants by L. acidophilus derived biosurfactant, 2019, 19, 1471-2180, 10.1186/s12866-019-1412-z
    45. Thaina A. Lima, Augusto Etchegaray, M. Teresa Machini, Design, synthesis and valued properties of surfactin oversimplified analogues, 2020, 52, 0939-4451, 25, 10.1007/s00726-019-02806-2
    46. Geeta Rawat, Anupam Dhasmana, Vivek Kumar, Biosurfactants: the next generation biomolecules for diverse applications, 2020, 3, 2523-8922, 353, 10.1007/s42398-020-00128-8
    47. Louiza Derguine-Mecheri, Salima Kebbouche-Gana, Djamel Djenane, Biosurfactant production from newly isolated Rhodotorula sp.YBR and its great potential in enhanced removal of hydrocarbons from contaminated soils, 2021, 37, 0959-3993, 10.1007/s11274-020-02983-3
    48. T.P. Pirog, T.A. Shevchuk, N.M. Petrenko, O.I. Paliichuk, G.O. Iutynska, Influence of Cultivation Conditions of Rhodococcus erythropolis IMV Ac-5017 on the Properties of Synthesized Surfactants, 2018, 80, 10280987, 13, 10.15407/microbiolj80.04.013
    49. Mohamed Elshikh, Scott Funston, Alif Chebbi, Syed Ahmed, Roger Marchant, Ibrahim M. Banat, Rhamnolipids from non-pathogenic Burkholderia thailandensis E264: Physicochemical characterization, antimicrobial and antibiofilm efficacy against oral hygiene related pathogens, 2017, 36, 18716784, 26, 10.1016/j.nbt.2016.12.009
    50. Chiara Ceresa, Maurizio Rinaldi, Valeria Chiono, Irene Carmagnola, Gianna Allegrone, Letizia Fracchia, Lipopeptides from Bacillus subtilis AC7 inhibit adhesion and biofilm formation of Candida albicans on silicone, 2016, 109, 0003-6072, 1375, 10.1007/s10482-016-0736-z
    51. Scott J. Funston, Konstantina Tsaousi, Thomas J. Smyth, Matthew S. Twigg, Roger Marchant, Ibrahim M. Banat, Enhanced rhamnolipid production in Burkholderia thailandensis transposon knockout strains deficient in polyhydroxyalkanoate (PHA) synthesis, 2017, 101, 0175-7598, 8443, 10.1007/s00253-017-8540-x
    52. Amedea Perfumo, Michelle Rudden, Roger Marchant, Ibrahim M. Banat, 2017, Chapter 35-1, 978-3-319-20796-4, 1, 10.1007/978-3-319-20796-4_35-1
    53. Chiara Ceresa, Letizia Fracchia, Emanuele Fedeli, Chiara Porta, Ibrahim M. Banat, Recent Advances in Biomedical, Therapeutic and Pharmaceutical Applications of Microbial Surfactants, 2021, 13, 1999-4923, 466, 10.3390/pharmaceutics13040466
    54. Kenia Barrantes, Juan José Araya, Luz Chacón, Rolando Procupez‐Schtirbu, Fernanda Lugo, Gabriel Ibarra, Víctor H. Soto, 2021, 9781119671022, 245, 10.1002/9781119671022.ch11
    55. Maria H. Ribeiro, Eva Fahr, Sara Lopes, 2022, 9781119769576, 1, 10.1002/9781119769620.ch1
    56. Marcos André Moura Dias, Marcia Nitschke, Bacterial-derived surfactants: an update on general aspects and forthcoming applications, 2023, 54, 1517-8382, 103, 10.1007/s42770-023-00905-7
    57. JINDARAT EKPRASERT, SASIWIMON KANAKAI, SULADDA YOSPRASONG, Improved Biosurfactant Production by Enterobacter cloacae B14, Stability Studies, and its Antimicrobial Activity, 2020, 69, 2544-4646, 273, 10.33073/pjm-2020-030
    58. Neelam Mishra, Kavita Rana, Siva Deepthi Seelam, Rakesh Kumar, Vijyendra Pandey, Bharathi P. Salimath, Dayanand Agsar, Characterization and Cytotoxicity of Pseudomonas Mediated Rhamnolipids Against Breast Cancer MDA-MB-231 Cell Line, 2021, 9, 2296-4185, 10.3389/fbioe.2021.761266
    59. Zahraa Amer Hashim, Jean‐Yves Maillard, Melanie Jay Wilson, Rachel Jane Waddington, Determining the potential use of biosurfactants in preventing endodontic infections, 2022, 130, 0909-8836, 10.1111/eos.12900
    60. Monohar Hossain Mondal, Wasefa Begum, Ajaya Bhattarai, Dileep Kumar, Bula Singh, Bidyut Saha, 2023, 9780128242834, 57, 10.1016/B978-0-12-824283-4.00002-2
    61. Moumita Biswas (Sarkar), Abhijit Sarkar, 2022, 9780128229217, 303, 10.1016/B978-0-12-822921-7.00016-7
    62. Simms A. Adu, Matthew S. Twigg, Patrick J. Naughton, Roger Marchant, Ibrahim M. Banat, Characterisation of cytotoxicity and immunomodulatory effects of glycolipid biosurfactants on human keratinocytes, 2023, 107, 0175-7598, 137, 10.1007/s00253-022-12302-5
    63. Anna Parus, Tomasz Ciesielski, Marta Woźniak-Karczewska, Mariusz Ślachciński, Mikołaj Owsianiak, Łukasz Ławniczak, Andreas P. Loibner, Hermann J. Heipieper, Łukasz Chrzanowski, Basic principles for biosurfactant-assisted (bio)remediation of soils contaminated by heavy metals and petroleum hydrocarbons – A critical evaluation of the performance of rhamnolipids, 2023, 443, 03043894, 130171, 10.1016/j.jhazmat.2022.130171
    64. Victor E. Vera-Santander, Ricardo H. Hernández-Figueroa, María T. Jiménez-Munguía, Emma Mani-López, Aurelio López-Malo, Health Benefits of Consuming Foods with Bacterial Probiotics, Postbiotics, and Their Metabolites: A Review, 2023, 28, 1420-3049, 1230, 10.3390/molecules28031230
    65. Aarati Panchabhai, 2021, 9780128233801, 367, 10.1016/B978-0-12-823380-1.00010-1
    66. Shristi Handa, Yadu Aggarwal, Sanjeev Puri, Mary Chatterjee, Pharmaceutical prospects of biosurfactants produced from fungal species, 2022, 62, 0233-111X, 1307, 10.1002/jobm.202200327
    67. Bhoomika M. Karamchandani, Ameya A. Pawar, Sujit S. Pawar, Sahil Syed, Nishigandha S. Mone, Sunil G. Dalvi, Pattanathu K. S. M. Rahman, Ibrahim M. Banat, Surekha K. Satpute, Biosurfactants’ multifarious functional potential for sustainable agricultural practices, 2022, 10, 2296-4185, 10.3389/fbioe.2022.1047279
    68. Amrita Kumari Panda, Seeta Dewali, Rashi Miglani, Rojita Mishra, Surajit De Mandal, Satpal Singh Bisht, 2023, 9780323901130, 147, 10.1016/B978-0-323-90113-0.00006-7
    69. Sweeta Akbari, Nour Hamid Abdurahman, Viacheslau Kudrashou, 2023, Chapter 7, 978-3-031-21681-7, 137, 10.1007/978-3-031-21682-4_7
    70. Celia Abolnik, Christine Strydom, Debbie Landman, Reneé Pieterse, Identification of bacteria in the tracheal swabs of farmed ostriches and their effect on the viability of influenza A virus, 2021, 33, 1040-6387, 1089, 10.1177/10406387211034483
    71. Sherly Antony, T.U. Sukumaran, Prasanth Rathinam, Reshmy R., Parameswaran Binod, Ashok Pandey, Raveendran Sindhu, 2022, 9780323851466, 439, 10.1016/B978-0-323-85146-6.00017-6
    72. Marcia Nitschke, Crisiane Aparecida Marangon, Microbial surfactants in nanotechnology: recent trends and applications, 2022, 42, 0738-8551, 294, 10.1080/07388551.2021.1933890
    73. Charles Oluwaseun Adetunji, Olugbemi Tope Olaniyan, Osikemekha Anthony Anani, Abel Inobeme, Awotunde Oluwasegun Samson, Julius Kola Oloke, Wadzani Dauda Palnam, Sana Ali, 2022, 9780323851466, 175, 10.1016/B978-0-323-85146-6.00014-0
    74. N.V. Krishna Prasad, R. Jayakumar, P. Wagdevi, S. Ramesh, 2021, 9780128226964, 21, 10.1016/B978-0-12-822696-4.00021-8
    75. Soni Kumari Singh, Ashish Sachan, 2022, 9780128234433, 419, 10.1016/B978-0-12-823443-3.00007-7
    76. Manoj Kumar Sarangi, Sasmita Padhi, L.D. Patel, Goutam Rath, Sitansu Sekhar Nanda, Dong Kee Yi, Theranostic efficiency of biosurfactants against COVID-19 and similar viruses - A review, 2022, 76, 17732247, 103764, 10.1016/j.jddst.2022.103764
    77. O. V. Kisil, V. S. Trefilov, V. S. Sadykova, M. E. Zvereva, E. A. Kubareva, Surfactin: Its Biological Activity and Possibility of Application in Agriculture, 2023, 59, 0003-6838, 1, 10.1134/S0003683823010027
    78. John Adewole Alara, Oluwaseun Ruth Alara, 2024, 9780443132889, 307, 10.1016/B978-0-443-13288-9.00001-2
    79. John Adewole Alara, 2024, 9780443132889, 355, 10.1016/B978-0-443-13288-9.00022-X
    80. Ines Ayadi, Sarra Akermi, Mariem Louati, Ali Gargouri, Lotfi Mellouli, Mohamed Guerfali, Microbial bioactive compounds from oleaginous yeast culture: insights into molecular docking interactions and toxicity prediction, 2024, 2190-6815, 10.1007/s13399-024-05516-2
    81. Swapnashree Satapathy, Ananya Kuanar, Arun Kumar Pradhan, 2024, 9789815196924, 80, 10.2174/9789815196924124010007
    82. Md. Sekendar Ali, Eon-Bee Lee, Walter H. Hsu, Kyoungho Suk, Syed Al Jawad Sayem, H. M. Arif Ullah, Seung-Jin Lee, Seung-Chun Park, Probiotics and Postbiotics as an Alternative to Antibiotics: An Emphasis on Pigs, 2023, 12, 2076-0817, 874, 10.3390/pathogens12070874
    83. Subhodip Mondal, Animesh Acharjee, Bidyut Saha, Utilization of natural surfactants: An approach towards sustainable universe, 2022, 60, 2572-8288, 1, 10.1002/vjch.202100137
    84. Mariana Amaral Azevedo, Letícia Portugal do Nascimento, Maria dos Remédios Vieira-Neta, Iolanda Cristina Silveira Duarte, 2023, Chapter 6, 978-3-031-31229-8, 129, 10.1007/978-3-031-31230-4_6
    85. Madduri Madhuri, Shivaprakash M. Rudramurthy, Utpal Roy, Two promising Bacillus-derived antifungal lipopeptide leads AF4 and AF5 and their combined effect with fluconazole on the in vitro Candida glabrata biofilms, 2024, 15, 1663-9812, 10.3389/fphar.2024.1334419
    86. O. V. Kisil, V. S. Trefilov, V. S. Sadykova, M. E. Zvereva, Е. А. Kubareva, SURFACTIN: BIOLOGICAL ACTIVITY AND THE POSSIBILITY OF AGRICULTURE APPLICATION (REVIEW), 2023, 59, 0555-1099, 3, 10.31857/S0555109923010026
    87. Fatimazahra Kadiri, Abdelkarim Ezaouine, Mohammed Blaghen, Faiza Bennis, Fatima Chegdani, Antibiofilm potential of biosurfactant produced by Bacillus aerius against pathogen bacteria, 2024, 56, 18788181, 102995, 10.1016/j.bcab.2023.102995
    88. Twinkle Rout, Muchalika Satapathy, Pratyasha Panda, Sibani Sahoo, Arun Kumar Pradhan, 2024, 9789815196924, 54, 10.2174/9789815196924124010005
    89. Avinash P. Ingle, Shreshtha Saxena, Mangesh Moharil, Mahendra Rai, Silvio S. Da Silva, 2023, 9781119854364, 173, 10.1002/9781119854395.ch9
    90. Karolína Englerová, Radomíra Nemcová, Eva Styková, Biosurfactants and their role in the inhibition of the biofilmforming pathogens, 2018, 67, 12107816, 107, 10.36290/csf.2018.015
    91. Francine Melise dos Santos, Amanda Pasinato Napp, Carolina Pinto de Aguiar, William Lautert Dutra, Breno GONÇALVES, Felipe Dalla Vecchia, Raj Deo Tewari, Clarissa Melo, Carbon Dioxide as a Carbon Source for Biosurfactant Production, 2025, 1556-5068, 10.2139/ssrn.5066224
    92. Priya Yadav, Rahul Prasad Singh, Subhasha Nigam, Rajpal Srivastav, Rachana Singh, Abeer Hasem, Elsayed Fathi Abd_Allah, Amit Raj, Sandeep Kumar Singh, Ajay Kumar, 2025, 24689289, 10.1016/bs.apmp.2024.11.002
    93. Roshan Jaiswal, Padmanaban Velayudhaperumal Chellam, Rangabhashiyam Selvasembian, Critical review on revamping circular economy strategies for the co-production of biosurfactants and lipase from agro-industrial wastes through resource recovery and life cycle assessment, 2025, 196, 09619534, 107733, 10.1016/j.biombioe.2025.107733
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