The use of metabolic engineering to produce fatty acid-derived biofuel and chemicals in <em>Saccharomyces cerevisiae</em>: a review

Liwei Chen; Jaslyn Lee; Wei Ning Chen; Liwei Chen; Jaslyn Lee; Wei Ning Chen

doi:10.3934/bioeng.2016.4.468

AIMS Bioengineering

2016, Volume 3, Issue 4: 468-492. doi: 10.3934/bioeng.2016.4.468

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The use of metabolic engineering to produce fatty acid-derived biofuel and chemicals in Saccharomyces cerevisiae: a review

School of Chemical and Biomedical Engineering, Nanyang Technological University, 62 Nanyang Drive, 637459, Singapore

Received: 30 September 2016 Accepted: 21 November 2016 Published: 25 November 2016

Production of fatty acid-derived biofuels and chemicals have garnered attention in recent years owing to their potential to replace petroleum and plant oil-derived products. Through the metabolic engineering of the fatty acid metabolism pathway, advanced fuels and chemicals such as free fatty acid, triacylglycerol, biodiesel, fatty alcohols, alkanes/alkene, R-3-hydroxybutyric acid, polyhydroxyalkanoates and flavonoids have been produced. The robustness, high tolerance to organic solvent, good reputation in industrial fermentations and excellent availability of genetic tools make the yeast Saccharomyces cerevisiae a suitable cell factory for fatty acid-derived biofuels and chemicals production. This review will describe the successful metabolic engineering strategies employed to produce the fatty acid-derived bio-products in S. cerevisiae, including the enhancement of precursors and co-factors supply, promotion of the enzyme expression and activity, elimination of competing pathways, and the improvement of strain tolerance.
- Saccharomyces cerevisiae,
- metabolic engineering,
- fatty acid metabolism,
- biofuels and chemicals
Citation: Liwei Chen, Jaslyn Lee, Wei Ning Chen. The use of metabolic engineering to produce fatty acid-derived biofuel and chemicals in Saccharomyces cerevisiae: a review[J]. AIMS Bioengineering, 2016, 3(4): 468-492. doi: 10.3934/bioeng.2016.4.468

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Abstract

Production of fatty acid-derived biofuels and chemicals have garnered attention in recent years owing to their potential to replace petroleum and plant oil-derived products. Through the metabolic engineering of the fatty acid metabolism pathway, advanced fuels and chemicals such as free fatty acid, triacylglycerol, biodiesel, fatty alcohols, alkanes/alkene, R-3-hydroxybutyric acid, polyhydroxyalkanoates and flavonoids have been produced. The robustness, high tolerance to organic solvent, good reputation in industrial fermentations and excellent availability of genetic tools make the yeast Saccharomyces cerevisiae a suitable cell factory for fatty acid-derived biofuels and chemicals production. This review will describe the successful metabolic engineering strategies employed to produce the fatty acid-derived bio-products in S. cerevisiae, including the enhancement of precursors and co-factors supply, promotion of the enzyme expression and activity, elimination of competing pathways, and the improvement of strain tolerance.

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