Characterization and analysis of myosin gene family in the whitefly (<i>Bemisia tabaci</i>)

Kui Wang; Zhifang Yang; Xiaohui Chen; Shunxiao Liu; Xiang Li; Liuhao Wang; Hao Yu; Hongwei Zhang; Kui Wang; Zhifang Yang; Xiaohui Chen; Shunxiao Liu; Xiang Li; Liuhao Wang; Hao Yu; Hongwei Zhang

doi:10.3934/molsci.2022006

AIMS Molecular Science

2022, Volume 9, Issue 2: 91-106. doi: 10.3934/molsci.2022006

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Research article

Characterization and analysis of myosin gene family in the whitefly (Bemisia tabaci)

1.
Department of Plant Protection, College of Resources and Environment, Henan Institute of Science and Technology, Xinxiang 453003, Henan Province, China
2.
College of Agrarian Technology and Natural Resources, Sumy National Agrarian University, Sumy, Ukraine

Received: 20 March 2022 Revised: 06 June 2022 Accepted: 15 June 2022 Published: 17 June 2022

Myosin is an actin-based motor protein that widely exists in muscle tissue and non-muscle tissue, and myosin of a diverse subfamily has obvious differences in structure and cell function. Many eukaryotes and even some unicellular organisms possess a variety of myosins. They have been well characterized in human, fungi and other organisms. However, the myosin gene family in Bemisia tabaci MEAM1 (Middle East-Asia Minor1 species) is poorly studied. In the study, we identified 15 myosin genes in B. tabaci MEAM1 based on a genome database. Myosin genes can be divided into ten classes, including subfamilies I, II, III, V, VI, VII, IX, XV, XVIII, XX in B. tabaci MEAM1. The amounts of myosin in Class I are the largest of the isoforms. Expression profiling of myosins by quantitative real-time PCR revealed that their expression differed among developmental stages and different tissues of B. tabaci MEAM1. The diversely may be related to the development characteristics of B. tabaci MEAM1. The BtaMyo-IIIb-like X1 was highly expressed in nymphs 4 instar which may be related to the development process before metamorphosis. Our outcome contributes to the basis for further research on myosin gene function in B. tabaci MEAM1 and homologous myosins in other biology.
- myosin,
- phylogenetic analysis,
- expression profiles,
- Bemisia tabaci
Citation: Kui Wang, Zhifang Yang, Xiaohui Chen, Shunxiao Liu, Xiang Li, Liuhao Wang, Hao Yu, Hongwei Zhang. Characterization and analysis of myosin gene family in the whitefly (Bemisia tabaci)[J]. AIMS Molecular Science, 2022, 9(2): 91-106. doi: 10.3934/molsci.2022006

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Abstract

Myosin is an actin-based motor protein that widely exists in muscle tissue and non-muscle tissue, and myosin of a diverse subfamily has obvious differences in structure and cell function. Many eukaryotes and even some unicellular organisms possess a variety of myosins. They have been well characterized in human, fungi and other organisms. However, the myosin gene family in Bemisia tabaci MEAM1 (Middle East-Asia Minor1 species) is poorly studied. In the study, we identified 15 myosin genes in B. tabaci MEAM1 based on a genome database. Myosin genes can be divided into ten classes, including subfamilies I, II, III, V, VI, VII, IX, XV, XVIII, XX in B. tabaci MEAM1. The amounts of myosin in Class I are the largest of the isoforms. Expression profiling of myosins by quantitative real-time PCR revealed that their expression differed among developmental stages and different tissues of B. tabaci MEAM1. The diversely may be related to the development characteristics of B. tabaci MEAM1. The BtaMyo-IIIb-like X1 was highly expressed in nymphs 4 instar which may be related to the development process before metamorphosis. Our outcome contributes to the basis for further research on myosin gene function in B. tabaci MEAM1 and homologous myosins in other biology.

Acknowledgments

This research was funded by the National Natural Science Foundation of China (31272103 and 31101497). I would like to thank comments from any anonymous reviewer helped improve the manuscript. All authors had full access to all data in the study and take responsibility for the integrity of the data. Hao Yu, Hongwei Zhang and Kui Wang conceived and designed the experiments. Kui Wang performed the study. Kui Wang, Zhifang Yang, Xiaohui Chen, Shunxiao Liu analyzed the data. Liuhao Wang contribution materials. Kui Wang, Zhifang Yang, Hao Yu, Hongwei Zhang edited and revised the manuscript.

Conflict of interest

The authors declare no competing or financial interests.

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