Research article

Influence of menthol on membrane-associated properties of tetracycline-resistant Escherichia coli

  • Received: 12 July 2024 Revised: 29 August 2024 Accepted: 03 September 2024 Published: 05 September 2024
  • In this investigation, we assessed the antibacterial properties of menthol using Escherichia coli strains, including a tetracycline-resistant (BN407) and a non-resistant reference (K12) strains. The disc-diffusion assay indicated remarkable antibacterial activity of menthol, warranting further exploration. The minimum inhibitory concentration (MIC) for menthol was determined to be 500 µg/ml for both strains. Despite identical MIC values, menthol exhibited different effects on the colony-forming units (CFUs) of the strains, reducing CFUs by 55% in E. coli K12 and 40% in E. coli BN407.

    Growth kinetics studies revealed that menthol extended the Lag phase by 50% and decreased the specific growth rate and mean generation time by nearly 50% for both strains. These findings illustrate menthol's significant impact on bacterial replication and adaptation processes. Additionally, menthol disrupted membrane-associated properties, as evidenced by reduced H+-flux through bacterial membranes, affecting both N,N-Dicyclohexylcarbodiimide (DCCD)-sensitive and non-sensitive proton flux rates. This indicates that menthol compromises the proton motive force critical for ATP synthesis and nutrient transport.

    In summary, menthol demonstrates potent antibacterial activity, influencing bacterial growth and survival. This activity is supposed to be due to the influence on membrane functionality. These effects are consistent across both tetracycline-resistant and non-resistant E. coli strains.

    Citation: Silvard Tadevosyan, Naira Sahakyan. Influence of menthol on membrane-associated properties of tetracycline-resistant Escherichia coli[J]. AIMS Biophysics, 2024, 11(3): 329-339. doi: 10.3934/biophy.2024018

    Related Papers:

  • In this investigation, we assessed the antibacterial properties of menthol using Escherichia coli strains, including a tetracycline-resistant (BN407) and a non-resistant reference (K12) strains. The disc-diffusion assay indicated remarkable antibacterial activity of menthol, warranting further exploration. The minimum inhibitory concentration (MIC) for menthol was determined to be 500 µg/ml for both strains. Despite identical MIC values, menthol exhibited different effects on the colony-forming units (CFUs) of the strains, reducing CFUs by 55% in E. coli K12 and 40% in E. coli BN407.

    Growth kinetics studies revealed that menthol extended the Lag phase by 50% and decreased the specific growth rate and mean generation time by nearly 50% for both strains. These findings illustrate menthol's significant impact on bacterial replication and adaptation processes. Additionally, menthol disrupted membrane-associated properties, as evidenced by reduced H+-flux through bacterial membranes, affecting both N,N-Dicyclohexylcarbodiimide (DCCD)-sensitive and non-sensitive proton flux rates. This indicates that menthol compromises the proton motive force critical for ATP synthesis and nutrient transport.

    In summary, menthol demonstrates potent antibacterial activity, influencing bacterial growth and survival. This activity is supposed to be due to the influence on membrane functionality. These effects are consistent across both tetracycline-resistant and non-resistant E. coli strains.


    Abbreviations

    EO

    essential oil

    CAGR

    compound annual growth rate

    SGR

    Specific growth rate

    WT

    wild type

    SD

    standard deviation

    MIC

    minimal inhibitory concentration

    DCCD

    N,N-Dicyclohexylcarbodiimide

    GS-MS

    gas chromatography mass spectrometry

    加载中

    Acknowledgments



    The research was supported by the Science Committee of MESCS RA, in the frames of the research projects №23AA-1F012.

    Conflict of interest



    There was no any conflict of interest.

    Author contributions



    ST carried out the research and created figures, NS supervised the research, analyzed data, and composed drafted version of article. Both authors approved the final version of article.

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