Cell growth on electrospun nanofiber mats from polyacrylonitrile (PAN) blends

Daria Wehlage; Hannah Blattner; Al Mamun; Ines Kutzli; Elise Diestelhorst; Anke Rattenholl; Frank Gudermann; Dirk Lütkemeyer; Andrea Ehrmann; Daria Wehlage; Hannah Blattner; Al Mamun; Ines Kutzli; Elise Diestelhorst; Anke Rattenholl; Frank Gudermann; Dirk Lütkemeyer; Andrea Ehrmann

doi:10.3934/bioeng.2020004

AIMS Bioengineering

2020, Volume 7, Issue 1: 43-54. doi: 10.3934/bioeng.2020004

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Cell growth on electrospun nanofiber mats from polyacrylonitrile (PAN) blends

1.
Faculty of Engineering and Mathematics, Bielefeld University of Applied Sciences, Bielefeld, Germany
2.
Institute of Food Science and Biotechnology, Department of Food Physics and Meat Science, University of Hohenheim, Stuttgart, Germany

Received: 14 January 2020 Accepted: 12 March 2020 Published: 16 March 2020

Nanofiber mats can be produced by electrospinning from diverse polymers and polymer blends as well as with embedded ceramics, metals, etc. The large surface-to-volume ratio makes such nanofiber mats a well-suited substrate for tissue engineering and other cell growth experiments. Cell growth, however, is not only influenced by the substrate morphology, but also by the sterilization process applied before the experiment as well as by the chemical composition of the fibers. A former study showed that cell growth and adhesion are supported by polyacrylonitrile/gelatin nanofiber mats, while both factors are strongly reduced on pure polyacrylonitrile (PAN) nanofibers. Here we report on the influence of different PAN blends on cell growth and adhesion. Our study shows that adding ZnO to the PAN spinning solution impedes cell growth, while addition of maltodextrin/pea protein or casein/gelatin supports cell growth and adhesion.
- electrospinning,
- nanofiber mat,
- autoclaving,
- cell growth,
- adherent cells,
- CHO cells,
- DMSO
Citation: Daria Wehlage, Hannah Blattner, Al Mamun, Ines Kutzli, Elise Diestelhorst, Anke Rattenholl, Frank Gudermann, Dirk Lütkemeyer, Andrea Ehrmann. Cell growth on electrospun nanofiber mats from polyacrylonitrile (PAN) blends[J]. AIMS Bioengineering, 2020, 7(1): 43-54. doi: 10.3934/bioeng.2020004

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Abstract

Nanofiber mats can be produced by electrospinning from diverse polymers and polymer blends as well as with embedded ceramics, metals, etc. The large surface-to-volume ratio makes such nanofiber mats a well-suited substrate for tissue engineering and other cell growth experiments. Cell growth, however, is not only influenced by the substrate morphology, but also by the sterilization process applied before the experiment as well as by the chemical composition of the fibers. A former study showed that cell growth and adhesion are supported by polyacrylonitrile/gelatin nanofiber mats, while both factors are strongly reduced on pure polyacrylonitrile (PAN) nanofibers. Here we report on the influence of different PAN blends on cell growth and adhesion. Our study shows that adding ZnO to the PAN spinning solution impedes cell growth, while addition of maltodextrin/pea protein or casein/gelatin supports cell growth and adhesion.

Acknowledgments

This study was partly funded by the PhD funds and the HiF funds of Bielefeld University of Applied Sciences.

Conflict of interest

All authors declare no conflicts of interest in this paper.

References

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