Naringin ameliorates motor dysfunction and exerts neuroprotective role against vanadium-induced neurotoxicity

Adeshina O. Adekeye; Adedamola A. Fafure; Ayoola E. Ogunsemowo; Linus A. Enye; Olusola S. Saka; Oluwatosin O. Ogedengbe; Adeshina O. Adekeye; Adedamola A. Fafure; Ayoola E. Ogunsemowo; Linus A. Enye; Olusola S. Saka; Oluwatosin O. Ogedengbe

doi:10.3934/Neuroscience.2022031

AIMS Neuroscience

2022, Volume 9, Issue 4: 536-550. doi: 10.3934/Neuroscience.2022031

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Naringin ameliorates motor dysfunction and exerts neuroprotective role against vanadium-induced neurotoxicity

1.
Department of Anatomy, College of Medicine and Health Sciences, Afe Babalola University, Ado-Ekiti, Ekiti State, Nigeria
2.
Department of Anatomy, Faculty of Basic Medical Sciences, Federal University Oye-Ekiti, Ekiti State, Nigeria

Received: 05 September 2022 Revised: 19 December 2022 Accepted: 21 December 2022 Published: 26 December 2022

Exposure to vanadium has been known to lead to a progressive neurodegenerative disorder like Parkinson's disease. Naringin is a known flavonoid glycoside that is mostly seen in the flesh of grapefruit and orange and is believed to have protective effects for the treatment of neurodegenerative disorders. This study sought to investigate the role of Naringin in the treatment of vanadium-induced neurotoxicity. Vanadium (10 mg/kg BW) was injected intraperitoneally to induce motor dysfunction, followed by treatment with Naringin (30 mg/kg BW) intraperitoneally for 14 days. Oxidative stress imbalance was monitored by checking Glutathione Peroxidase (GPX) and Catalase levels. Histological and immunohistochemical alterations were observed using RBFOX3 polyclonal antibody to determine neuronal cell distribution and NLRP3 inflammasome antibody as a marker of inflammation. Exposure to vanadium induces neurotoxicity by significantly increasing the Catalase and Glutathione Peroxidase (GPX) levels. Vanadium administration also led to increased inflammatory cells and a significant reduction of the viable neuronal cells in the SNc and CPu. Treatment with Naringin showed a neuroprotective role by dependently restoring the Catalase and Glutathione Peroxidase (GPX) levels, inflammasome activation, and neuronal damage in the SNc and CPu. Naringin demonstrated anti-oxidative, and anti-inflammatory responses by inhibiting oxidative stress, and inflammation and exerts neuroprotective effects by inhibiting apoptosis following vanadium-induced neurotoxicity in adult Wistar rats.
- substantia nigra,
- caudate putamen,
- Parkinson's disease,
- vanadium,
- Naringin
Citation: Adeshina O. Adekeye, Adedamola A. Fafure, Ayoola E. Ogunsemowo, Linus A. Enye, Olusola S. Saka, Oluwatosin O. Ogedengbe. Naringin ameliorates motor dysfunction and exerts neuroprotective role against vanadium-induced neurotoxicity[J]. AIMS Neuroscience, 2022, 9(4): 536-550. doi: 10.3934/Neuroscience.2022031

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Abstract

Exposure to vanadium has been known to lead to a progressive neurodegenerative disorder like Parkinson's disease. Naringin is a known flavonoid glycoside that is mostly seen in the flesh of grapefruit and orange and is believed to have protective effects for the treatment of neurodegenerative disorders. This study sought to investigate the role of Naringin in the treatment of vanadium-induced neurotoxicity. Vanadium (10 mg/kg BW) was injected intraperitoneally to induce motor dysfunction, followed by treatment with Naringin (30 mg/kg BW) intraperitoneally for 14 days. Oxidative stress imbalance was monitored by checking Glutathione Peroxidase (GPX) and Catalase levels. Histological and immunohistochemical alterations were observed using RBFOX3 polyclonal antibody to determine neuronal cell distribution and NLRP3 inflammasome antibody as a marker of inflammation. Exposure to vanadium induces neurotoxicity by significantly increasing the Catalase and Glutathione Peroxidase (GPX) levels. Vanadium administration also led to increased inflammatory cells and a significant reduction of the viable neuronal cells in the SNc and CPu. Treatment with Naringin showed a neuroprotective role by dependently restoring the Catalase and Glutathione Peroxidase (GPX) levels, inflammasome activation, and neuronal damage in the SNc and CPu. Naringin demonstrated anti-oxidative, and anti-inflammatory responses by inhibiting oxidative stress, and inflammation and exerts neuroprotective effects by inhibiting apoptosis following vanadium-induced neurotoxicity in adult Wistar rats.

Abbreviations

GPX

Glutathione Peroxidase

Endoplasmic Reticulum

VAN

Vanadium

NAR

Naringin

SNc

Substantia nigra pars compacta

CPu

Caudate putamen

GDNF

Glial cell line-derived neurotrophic factor

TNF

Tumour Necrosis Factor

Conflict of interest

All author declare no conflict of interest.

Author contributions

AOA conceived the conceptual idea. AAF, AEO and OSS conducted the experiments and planned the literature search. AAF and OOO analyzed and interpreted the data. AAF, AOA and LAE drafted the manuscript, read, and revised the manuscript. All authors gave the final approval for the manuscript to be published.

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