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AIMS Genetics

2016, Volume 3, Issue 4: 196-204. doi: 10.3934/genet.2016.4.196
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Analysis of class 2 integrons as a marker for multidrug resistance among Gram negative bacilli

  • 1.
    Instituto de Microbiología y Parasitología Médica, Universidad de Buenos Aires-Consejo Nacional de Investigaciones Científicas y Técnicas (IMPaM, UBA-CONICET), Buenos Aires, Argentina
  • 2.
    Centro de Referencia para Lactobacilos (CERELA-CONICET), Tucumán, Argentina
  • 3.
    Grupo GEMA, DCB, Universidad Nacional de Luján, Buenos Aires, Argentina y Laboratorio de Biología del Comportamiento, IBYME, Buenos Aires, Argentina
  • 4.
    Hospital del Niño Jesús, Tucumán, Argentina
  • 5.
    Center for Applied Biotechnology Studies, Department of Biological Science, California State University Fullerton, Fullerton, California
  • Received: 25 April 2016 Accepted: 25 September 2016 Published: 09 October 2016

  • Class 1 and 2 integrons are considered the paradigm of multidrug resistant (MDR) integrons. Although class 1 integrons have been found statistically associated to Enterobacteriaceae MDR isolates, this type of study has not been conducted for class 2 integrons. and 3 species that were found that harbored more than 20% of class 2 integrons in clinical isolates, were selected to determine the role of intI2 as MDR marker. A total of 234 MDR/191 susceptible non-epidemiologically related isolates were analyzed. Seventy-four intI2 genes were found by PCR and sequencing. An intI2 relationship with MDR phenotypes in Acinetobacter baumannii and Enterobacter cloacae was found. No statistical association was identified with MDR E. coli and Helicobacter pylori isolates. In other words, the likelihood of finding intI2 is the same in susceptible and in MDR E. coli and H. pylori strains, suggesting a particular affinity between the mobile element Tn7 and some species. The use of intI2 as MDR marker was species-dependent, with fluctuating epidemiology at geographical and temporal gradients. The use of intI2 as MDR marker is advisable in A. baumannii, a species that can reach high frequencies of this genetic element.

    Citation: Cecilia Rodríguez, Marcelo H. Cassini, Gabriela del V. Delgado, Argentinian Integron Study Group, María S. Ramírez, D. Centrón. Analysis of class 2 integrons as a marker for multidrug resistance among Gram negative bacilli[J]. AIMS Genetics, 2016, 3(4): 196-204. doi: 10.3934/genet.2016.4.196

    Related Papers:

  • Class 1 and 2 integrons are considered the paradigm of multidrug resistant (MDR) integrons. Although class 1 integrons have been found statistically associated to Enterobacteriaceae MDR isolates, this type of study has not been conducted for class 2 integrons. and 3 species that were found that harbored more than 20% of class 2 integrons in clinical isolates, were selected to determine the role of intI2 as MDR marker. A total of 234 MDR/191 susceptible non-epidemiologically related isolates were analyzed. Seventy-four intI2 genes were found by PCR and sequencing. An intI2 relationship with MDR phenotypes in Acinetobacter baumannii and Enterobacter cloacae was found. No statistical association was identified with MDR E. coli and Helicobacter pylori isolates. In other words, the likelihood of finding intI2 is the same in susceptible and in MDR E. coli and H. pylori strains, suggesting a particular affinity between the mobile element Tn7 and some species. The use of intI2 as MDR marker was species-dependent, with fluctuating epidemiology at geographical and temporal gradients. The use of intI2 as MDR marker is advisable in A. baumannii, a species that can reach high frequencies of this genetic element.


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  • © 2016 the Author(s), licensee AIMS Press. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0)
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