Down-regulation of IRF3 expression in Relapse-Remitting MS patients

Sobhan Helbi; Zahra Engardeh; Sahar Nickbin Poshtamsary; Zaynab Aminzadeh; Nahid Jivad; Sobhan Helbi; Zahra Engardeh; Sahar Nickbin Poshtamsary; Zaynab Aminzadeh; Nahid Jivad

doi:10.3934/medsci.2019.2.140

AIMS Medical Science

2019, Volume 6, Issue 2: 140-147. doi: 10.3934/medsci.2019.2.140

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Down-regulation of IRF3 expression in Relapse-Remitting MS patients

1.
School of Medicine, Dezful University of Medical Sciences, Dezful, Iran
2.
Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran
3.
School of Nursing and Midwifery, Guilan University of Medical Sciences, Rasht, Iran
4.
Department of Biochemistry, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
5.
Department of Neurology, Faculty of Medicine, Shahrekord University of Medical Sciences, Shahrekord, Iran

Received: 25 February 2019 Accepted: 24 April 2019 Published: 14 May 2019

Background: Relapsing-Remitting (RRMS) is the most common Multiple Sclerosis disease course. Interferon regulatory factor 3 (IRF3) as major regulators of immune system genes plays a critical role in the activation of type I interferons promoters, in particular IFNβ promoter. Hence we aimed to evaluate the expression rate of IRF3 in RRMS patients under different type of IFNβ treatment. Material and methods: In the present study total of 100 subjects participated. Blood samples of 25 patients with RRMS newly diagnosed who have not been treated with interferon components, 25 patients with RRMS treated with Interferon beta-1α (B1a), 25 patients with RRMS treated with Interferon beta-1β (B1b) and 25 control samples were collected. The samples were transferred at standard conditions to the Cellular and Molecular Research Center of Shahrekord University of Medical Sciences, RNA was extracted and converted to cDNA. To evaluate the expression of IRF3 the Real-Time PCR method using SYBR Green dye was done. The level of gene expression was measured by a comparative threshold cycle formula. The obtained data were analyzed using SPSS v15 software. Results: In the study we compared the IRF3 mRNA expression of all subjects in association with gender, which no significant difference was seen (P > 0.05). Also assessment of the gene mRNA level in study groups revealed that the B1b, B1a and new case group had the lowest expression respectively. Moreover, comparison of the mRNA level between new case and B1b groups showed remarkable difference (P < 0.05). According to age and sex factors, no remarkable differences between study groups were seen (P > 0.05). Conclusion: Perhaps the IFNβ recombinants decreases the IRF3 expression as a negative feedback mechanism. Overall the data reported here, supports the previous studies in important role of IRF3 in autoimmune inflammatory disease of CNS and Multiple Sclerosis.
- multiple sclerosis,
- IRF3,
- IFNβ
Citation: Sobhan Helbi, Zahra Engardeh, Sahar Nickbin Poshtamsary, Zaynab Aminzadeh, Nahid Jivad. Down-regulation of IRF3 expression in Relapse-Remitting MS patients[J]. AIMS Medical Science, 2019, 6(2): 140-147. doi: 10.3934/medsci.2019.2.140

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Abstract

Background: Relapsing-Remitting (RRMS) is the most common Multiple Sclerosis disease course. Interferon regulatory factor 3 (IRF3) as major regulators of immune system genes plays a critical role in the activation of type I interferons promoters, in particular IFNβ promoter. Hence we aimed to evaluate the expression rate of IRF3 in RRMS patients under different type of IFNβ treatment. Material and methods: In the present study total of 100 subjects participated. Blood samples of 25 patients with RRMS newly diagnosed who have not been treated with interferon components, 25 patients with RRMS treated with Interferon beta-1α (B1a), 25 patients with RRMS treated with Interferon beta-1β (B1b) and 25 control samples were collected. The samples were transferred at standard conditions to the Cellular and Molecular Research Center of Shahrekord University of Medical Sciences, RNA was extracted and converted to cDNA. To evaluate the expression of IRF3 the Real-Time PCR method using SYBR Green dye was done. The level of gene expression was measured by a comparative threshold cycle formula. The obtained data were analyzed using SPSS v15 software. Results: In the study we compared the IRF3 mRNA expression of all subjects in association with gender, which no significant difference was seen (P > 0.05). Also assessment of the gene mRNA level in study groups revealed that the B1b, B1a and new case group had the lowest expression respectively. Moreover, comparison of the mRNA level between new case and B1b groups showed remarkable difference (P < 0.05). According to age and sex factors, no remarkable differences between study groups were seen (P > 0.05). Conclusion: Perhaps the IFNβ recombinants decreases the IRF3 expression as a negative feedback mechanism. Overall the data reported here, supports the previous studies in important role of IRF3 in autoimmune inflammatory disease of CNS and Multiple Sclerosis.

Acknowledgments

The results of this article are extracted from a dissertation approved by council of Shahrekord University of Medical Sciences (with code of 1965). The researcher, herby, appreciated project colleagues, the staff of the Student Research Committee and members of the research council of Shahrekord University of Medical Sciences who approved the project and presented their sincere cooperation.

Conflict of interest

The author declares no conflicts of interest in this paper.

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