Plant beneficial traits of endophytic bacteria associated with fennel (<i>Foeniculum vulgare</i> Mill.)

Vyacheslav Shurigin; Li Li; Burak Alaylar; Dilfuza Egamberdieva; Yong-Hong Liu; Wen-Jun Li; Vyacheslav Shurigin; Li Li; Burak Alaylar; Dilfuza Egamberdieva; Yong-Hong Liu; Wen-Jun Li

doi:10.3934/microbiol.2024022

AIMS Microbiology

2024, Volume 10, Issue 2: 449-467. doi: 10.3934/microbiol.2024022

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Research article

Plant beneficial traits of endophytic bacteria associated with fennel (Foeniculum vulgare Mill.)

1.
State Key Laboratory of Desert and Oasis Ecology, Xinjiang Key Laboratory of Biodiversity Conservation and Application in Arid Lands, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, Xinjiang, China
2.
Department of Molecular Biology and Genetics, Faculty of Arts and Sciences, Agri Ibrahim Cecen University, Agri 04100, Turkey
3.
Institute of Fundamental and Applied Research, National Research University TIIAME, Tashkent 100000, Uzbekistan
4.
Faculty of Biology, National University of Uzbekistan, Tashkent 100174, Uzbekistan
5.
State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Stress Biology, Sun Yat-Sen University, Guangzhou, 510275, China

Received: 26 November 2023 Revised: 03 June 2024 Accepted: 12 June 2024 Published: 18 June 2024

In this study, we used 16S rRNA gene sequence analysis to describe the diversity of cultivable endophytic bacteria associated with fennel (Foeniculum vulgare Mill.) and determined their plant-beneficial traits. The bacterial isolates from the roots of fennel belonged to four phyla: Firmicutes (BRN1 and BRN3), Proteobacteria (BRN5, BRN6, and BRN7), Gammaproteobacteria (BRN2), and Actinobacteria (BRN4). The bacterial isolates from the shoot of fennel represented the phyla Proteobacteria (BSN1, BSN2, BSN3, BSN5, BSN6, BSN7, and BSN8), Firmicutes (BSN4, BRN1, and BRN3), and Actinobacteria (BRN4). The bacterial species Bacillus megaterium, Bacillus aryabhattai, and Brevibacterium frigoritolerans were found both in the roots and shoots of fennel. The bacterial isolates were found to produce siderophores, HCN, and indole-3-acetic acid (IAA), as well as hydrolytic enzymes such as chitinase, protease, glucanase, and lipase. Seven bacterial isolates showed antagonistic activity against Fusarium culmorum, Fusarium solani, and Rhizoctonia. solani. Our findings show that medicinal plants with antibacterial activity may serve as a source for the selection of microorganisms that exhibit antagonistic activity against plant fungal infections and may be considered as a viable option for the management of fungal diseases. They can also serve as an active part of biopreparation, improving plant growth.
- medicinal plant,
- plant beneficial,
- antagonism,
- endophytes
Citation: Vyacheslav Shurigin, Li Li, Burak Alaylar, Dilfuza Egamberdieva, Yong-Hong Liu, Wen-Jun Li. Plant beneficial traits of endophytic bacteria associated with fennel (Foeniculum vulgare Mill.)[J]. AIMS Microbiology, 2024, 10(2): 449-467. doi: 10.3934/microbiol.2024022

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Abstract

In this study, we used 16S rRNA gene sequence analysis to describe the diversity of cultivable endophytic bacteria associated with fennel (Foeniculum vulgare Mill.) and determined their plant-beneficial traits. The bacterial isolates from the roots of fennel belonged to four phyla: Firmicutes (BRN1 and BRN3), Proteobacteria (BRN5, BRN6, and BRN7), Gammaproteobacteria (BRN2), and Actinobacteria (BRN4). The bacterial isolates from the shoot of fennel represented the phyla Proteobacteria (BSN1, BSN2, BSN3, BSN5, BSN6, BSN7, and BSN8), Firmicutes (BSN4, BRN1, and BRN3), and Actinobacteria (BRN4). The bacterial species Bacillus megaterium, Bacillus aryabhattai, and Brevibacterium frigoritolerans were found both in the roots and shoots of fennel. The bacterial isolates were found to produce siderophores, HCN, and indole-3-acetic acid (IAA), as well as hydrolytic enzymes such as chitinase, protease, glucanase, and lipase. Seven bacterial isolates showed antagonistic activity against Fusarium culmorum, Fusarium solani, and Rhizoctonia. solani. Our findings show that medicinal plants with antibacterial activity may serve as a source for the selection of microorganisms that exhibit antagonistic activity against plant fungal infections and may be considered as a viable option for the management of fungal diseases. They can also serve as an active part of biopreparation, improving plant growth.

Acknowledgments

This research was supported by Xinjiang Uygur Autonomous Region regional coordinated innovation project (Shanghai cooperation organization science and technology partnership program) (Nos.: 2020E01047 and 2021E01018), National Natural Science Foundation of China (Nos.: 32050410306 and 32000084) and the Third Xinjiang Scientific Expedition Program (Grant No. 2022xjkk1204).

Conflict of interest

The authors declare no conflict of interest.

Author contributions

DE designed the experiment. VS and BA conducted the laboratory experiments. VS and LL analysed the results of experiments. DE, VS, and YHL wrote the manuscript. WJL revised the manuscript and made critical comments. All authors read and approved the manuscript.

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