Growth inhibition of cultured cancer cells by <i>Ribes nigrum</i> leaf extract

Mikayel Ginovyan; Agnieszka Bartoszek; Izabela Koss-Mikołajczyk; Barbara Kusznierewicz; Pierre Andreoletti; Mustapha Cherkaoui-Malki; Naira Sahakyan; Mikayel Ginovyan; Agnieszka Bartoszek; Izabela Koss-Mikołajczyk; Barbara Kusznierewicz; Pierre Andreoletti; Mustapha Cherkaoui-Malki; Naira Sahakyan

doi:10.3934/biophy.2022024

AIMS Biophysics

2022, Volume 9, Issue 3: 282-293. doi: 10.3934/biophy.2022024

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Growth inhibition of cultured cancer cells by Ribes nigrum leaf extract

1.
Department of Biochemistry, Microbiology & Biotechnology; Research Institute of Biology Yerevan State University, 1 A. Manoogian Str., 0025 Yerevan, Armenia
2.
Department of Food Chemistry, Technology and Biotechnology, Faculty of Chemistry, Gdańsk University of Technology, Narutowicza 11/12, 80–233, Gdańsk, Poland
3.
Laboratoire Bio-PeroxIL EA7270, University of Bourgogne Franche-Comté, 21000 Dijon, France

Received: 23 August 2022 Revised: 28 September 2022 Accepted: 10 October 2022 Published: 21 October 2022

The present article includes data on the possible selective cytotoxic effect of extract of Ribes nigrum L. growing at high Armenian landscape. For this purpose, different non-cancer (microglial BV-2 wild type (Wt), acyl-CoA oxidase 1 (ACOX1) deficient (Acox1^−/−) and cancer (human colon adenocarcinoma HT29 and human breast cancer MCF7) cell lines were applied. R. nigrum leaf ethanol extract showed a growth inhibition effect towards HT29 and MCF7 cells started from 6 h of treatment at the concentration of 0.5 mg/mL DW. The lowest concentration (0.125 mg/mL DW) of the investigated extract expressed cytotoxicity after 72 hours following cancer cell treatment. In contrast to the cancer cells, in the case of the tested non-cancer cells, cytotoxic effect was not observed at the applied concentrations. The extract sub-cytotoxic concentration, in this case, was reported to be the 1 mg/mL DW. Further investigations are needed to confirm the selective cytotoxicity and possible action mechanisms of the leaf extract of R. nigrum.
- plant extract,
- Armenian flora,
- cytotoxicity,
- microglia,
- human adenocarcinoma,
- breast cancer
Citation: Mikayel Ginovyan, Agnieszka Bartoszek, Izabela Koss-Mikołajczyk, Barbara Kusznierewicz, Pierre Andreoletti, Mustapha Cherkaoui-Malki, Naira Sahakyan. Growth inhibition of cultured cancer cells by Ribes nigrum leaf extract[J]. AIMS Biophysics, 2022, 9(3): 282-293. doi: 10.3934/biophy.2022024

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Abstract

The present article includes data on the possible selective cytotoxic effect of extract of Ribes nigrum L. growing at high Armenian landscape. For this purpose, different non-cancer (microglial BV-2 wild type (Wt), acyl-CoA oxidase 1 (ACOX1) deficient (Acox1^−/−) and cancer (human colon adenocarcinoma HT29 and human breast cancer MCF7) cell lines were applied. R. nigrum leaf ethanol extract showed a growth inhibition effect towards HT29 and MCF7 cells started from 6 h of treatment at the concentration of 0.5 mg/mL DW. The lowest concentration (0.125 mg/mL DW) of the investigated extract expressed cytotoxicity after 72 hours following cancer cell treatment. In contrast to the cancer cells, in the case of the tested non-cancer cells, cytotoxic effect was not observed at the applied concentrations. The extract sub-cytotoxic concentration, in this case, was reported to be the 1 mg/mL DW. Further investigations are needed to confirm the selective cytotoxicity and possible action mechanisms of the leaf extract of R. nigrum.

Abbreviations

ACOX1

acyl-CoA oxidase type 1

DMEM

Dulbecco's modified Eagle medium

DPPH

1,1-diphenyl-2-picrylhydrazyl

EMEM

Minimal Essential Medium Eagle

dry weight

EDTA

ethylenediamine tetraacetic acid

FBS

fetal bovine serum

GAE

gallic acid equivalent

MTT

3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide

PBS

phosphate-buffered saline

quercetin equivalent

VLCFA

very-long-chain fatty acids

wild type

Acknowledgments

This work was supported by the Science Committee of RA, in the frames of the research project № 21AG-4D027; Basic support from Science Committee of RA, Ministry of Education, Science, Culture and Sports of RA, as well as COST Action NutRedOx-CA16112 in the frames of STSM program.

Conflict of interest

All authors declare no conflict of interest.

Author contributions

All authors contributed to the article formation equally. Authors revised and approved the final version of the manuscript.

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