Influence of arabinoxylan and crosslinked arabinoxylan consumption on blood serum lipids and glucose levels of Wistar rats

Figueroa-Pizano María Dolores; Campa-Mada Alma Consuelo; Canett-Romero Rafael; Paz-Samaniego Rita; Martínez-López Ana Luisa; Carvajal-Millan Elizabeth; Figueroa-Pizano María Dolores; Campa-Mada Alma Consuelo; Canett-Romero Rafael; Paz-Samaniego Rita; Martínez-López Ana Luisa; Carvajal-Millan Elizabeth

doi:10.3934/bioeng.2021018

AIMS Bioengineering

2021, Volume 8, Issue 3: 208-220. doi: 10.3934/bioeng.2021018

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Influence of arabinoxylan and crosslinked arabinoxylan consumption on blood serum lipids and glucose levels of Wistar rats

1.
Biopolymers-CTAOA, Research Center for Food and Development (CIAD, AC). Carretera Gustavo Enrique Astiazarán Rosas No. 46, Hermosillo, Sonora 83304, Mexico
2.
University of Sonora, Rosales y Blvd. Luis D. Colosio, Hermosillo, Sonora 83000, Mexico
3.
NANO-VAC Research Group, Department of Chemistry and Pharmaceutical Technology, University of Navarra, 31008 Pamplona, Spain

Received: 18 May 2021 Accepted: 01 July 2021 Published: 12 July 2021

Several studies have described the health benefits of arabinoxylan as prebiotics; however, other authors have related them with an anti-nutrient effect as arabinoxylan increases the viscosity of the alimentary bolus. In this work, the impact of arabinoxylan and crosslinked arabinoxylan on blood serum lipids and glucose levels of Wistar rats was investigated. Arabinoxylan was extracted from maize bran, presented a Fourier Transform Infra-Red spectrum typical for this polysaccharide, and a molecular weight of 250 kDa. Arabinoxylan solution at 4% (w/v) formed covalent gels induced by laccase. Male Wistar rats were fed a standard diet supplemented with 5% (w/w) lyophilized arabinoxylan or crosslinked arabinoxylan. Blood glucose levels were determined, collecting a drop of blood from the tail vein of rats at 0, 2, and 10 h after food consumption. Total cholesterol, triglycerides, high-density lipoprotein (HDL) cholesterol, and low-density lipoprotein (LDL) cholesterol were also determined. Postprandial blood glucose of the treatment groups was maintained at the same level as the control group. The serum lipid profile levels also remained close to the control group, excepting total cholesterol and LDL-cholesterol, which were higher in crosslinked arabinoxylan treatment but in the range reported for this murine model. The obtained results revealed that consumption of arabinoxylan and crosslinked arabinoxylan at moderated levels does not interfere with the absorption of these nutrients.
- arabinoxylan,
- gels,
- blood glucose,
- serum lipids,
- viscosity
Citation: Figueroa-Pizano María Dolores, Campa-Mada Alma Consuelo, Canett-Romero Rafael, Paz-Samaniego Rita, Martínez-López Ana Luisa, Carvajal-Millan Elizabeth. Influence of arabinoxylan and crosslinked arabinoxylan consumption on blood serum lipids and glucose levels of Wistar rats[J]. AIMS Bioengineering, 2021, 8(3): 208-220. doi: 10.3934/bioeng.2021018

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Abstract

Several studies have described the health benefits of arabinoxylan as prebiotics; however, other authors have related them with an anti-nutrient effect as arabinoxylan increases the viscosity of the alimentary bolus. In this work, the impact of arabinoxylan and crosslinked arabinoxylan on blood serum lipids and glucose levels of Wistar rats was investigated. Arabinoxylan was extracted from maize bran, presented a Fourier Transform Infra-Red spectrum typical for this polysaccharide, and a molecular weight of 250 kDa. Arabinoxylan solution at 4% (w/v) formed covalent gels induced by laccase. Male Wistar rats were fed a standard diet supplemented with 5% (w/w) lyophilized arabinoxylan or crosslinked arabinoxylan. Blood glucose levels were determined, collecting a drop of blood from the tail vein of rats at 0, 2, and 10 h after food consumption. Total cholesterol, triglycerides, high-density lipoprotein (HDL) cholesterol, and low-density lipoprotein (LDL) cholesterol were also determined. Postprandial blood glucose of the treatment groups was maintained at the same level as the control group. The serum lipid profile levels also remained close to the control group, excepting total cholesterol and LDL-cholesterol, which were higher in crosslinked arabinoxylan treatment but in the range reported for this murine model. The obtained results revealed that consumption of arabinoxylan and crosslinked arabinoxylan at moderated levels does not interfere with the absorption of these nutrients.

Abbreviations

arabinoxylan

AX-Gel

crosslinked arabinoxylan

ferulic acid

HDL

high-density lipoprotein

LDL

low-density lipoprotein

A/X

arabinose to xylose ratio

FT-IR

Fourier transform infrared

KBr

potassium bromide

CIAD

Research Center for Food and Development

G′

storage modulus

G″

loss modulus

Acknowledgments

M.D Figueroa-Pizano acknowledges CONACyT for the support provided for postdoctoral research. This research was funded by ‘Fund to support research on the Sonora-Arizona region 2019’, Mexico. Grant number 70309.

Conflicts of interest

The authors declare no conflict of interest.

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