Research article

Effects of the maturity level and pod conditioning period of cocoa pods on the changes of physicochemical properties of the beans of Sulawesi 2 (S2) cocoa clone

  • Received: 01 January 2023 Revised: 30 March 2023 Accepted: 15 May 2023 Published: 29 May 2023
  • Cocoa quality largely depends on postharvest processing, including fruit maturity levels and practices, such as ripening (pod conditioning), fermentation, drying, and roasting. This study aimed to identify the effects of the maturity level and pod conditioning period of cocoa pods on the changes in the physicochemical properties of the beans of Sulawesi 2 (S2) cocoa clone from Pinrang Regency, South Sulawesi, Indonesia. Two treatment variables were applied and assessed: fruit maturity levels (treatment A) and duration of pod conditioning (treatment B). Results indicated that pulp weight ranged from 29.69–47.96 g/100 g fresh beans, pulp moisture was 74.43%–83.29%, total sugar content was 4.28%–11.91%, sucrose content was 0.33%–0.88%, glucose/fructose was 3.84%–11.09%, and cocoa pulp pH was 3.27–3.79. The bean moisture was 37.13%–58.67%, bean weight was 54.19–72.4 g/100 g fresh beans, and polyphenol content ranged from 4.76–13.05 mg/g in gallic acid equivalents. Statistical analyses indicated that fruit maturity level did not have a significant effect on the weight, moisture, total sugar content, glucose/fructose content or pH of the pulp or the moisture content and weight of the bean. However, fruit maturity level significantly affected the polyphenol and pulp sucrose contents of the bean. Furthermore, pod conditioning had significant effects on the weight, total sugar content, sucrose content, and glucose/fructose content of the pulp as well as the weight and polyphenol contents of the beans. The effects of pod conditioning on the moisture and pH of the pulp and the bean moisture content were insignificant. Pod conditioning for 6 days with an A2 maturity level not only reduced the acidity of the cocoa beans, but it also increased the polyphenol content and the weight of the beans. Increased bean weight will increase the yield of cocoa beans. Therefore, pod conditioning for 6 days with an A2 maturity level can be applied to the cocoa processing industry, especially before carrying out the cocoa bean fermentation.

    Citation: Nur Laylah, S. Salengke, Amran Laga, Supratomo Supratomo. Effects of the maturity level and pod conditioning period of cocoa pods on the changes of physicochemical properties of the beans of Sulawesi 2 (S2) cocoa clone[J]. AIMS Agriculture and Food, 2023, 8(2): 615-636. doi: 10.3934/agrfood.2023034

    Related Papers:

  • Cocoa quality largely depends on postharvest processing, including fruit maturity levels and practices, such as ripening (pod conditioning), fermentation, drying, and roasting. This study aimed to identify the effects of the maturity level and pod conditioning period of cocoa pods on the changes in the physicochemical properties of the beans of Sulawesi 2 (S2) cocoa clone from Pinrang Regency, South Sulawesi, Indonesia. Two treatment variables were applied and assessed: fruit maturity levels (treatment A) and duration of pod conditioning (treatment B). Results indicated that pulp weight ranged from 29.69–47.96 g/100 g fresh beans, pulp moisture was 74.43%–83.29%, total sugar content was 4.28%–11.91%, sucrose content was 0.33%–0.88%, glucose/fructose was 3.84%–11.09%, and cocoa pulp pH was 3.27–3.79. The bean moisture was 37.13%–58.67%, bean weight was 54.19–72.4 g/100 g fresh beans, and polyphenol content ranged from 4.76–13.05 mg/g in gallic acid equivalents. Statistical analyses indicated that fruit maturity level did not have a significant effect on the weight, moisture, total sugar content, glucose/fructose content or pH of the pulp or the moisture content and weight of the bean. However, fruit maturity level significantly affected the polyphenol and pulp sucrose contents of the bean. Furthermore, pod conditioning had significant effects on the weight, total sugar content, sucrose content, and glucose/fructose content of the pulp as well as the weight and polyphenol contents of the beans. The effects of pod conditioning on the moisture and pH of the pulp and the bean moisture content were insignificant. Pod conditioning for 6 days with an A2 maturity level not only reduced the acidity of the cocoa beans, but it also increased the polyphenol content and the weight of the beans. Increased bean weight will increase the yield of cocoa beans. Therefore, pod conditioning for 6 days with an A2 maturity level can be applied to the cocoa processing industry, especially before carrying out the cocoa bean fermentation.



    加载中


    [1] Utrilla-Vázquez M, Rodrigues-Campoz J, Avendano-Arazate CH, et al. (2020) Analysis of volatile compounds of five varieties of Maya cocoa during fermentation and drying processes by Venn diagram and PCA. Food Res Int 129: 108834. https://doi.org/10.1016/j.foodres.2019.108834 doi: 10.1016/j.foodres.2019.108834
    [2] Rojas KE, García MC, Cerón IX, et al. (2020) Identification of potential maturity indicators for harvesting cacao. Heliyon 6: E03416. https://doi.org/10.1016/j.heliyon.2020.e03416 doi: 10.1016/j.heliyon.2020.e03416
    [3] Biehl B, Meyer B, Said M Bin (1990) Bean spreading: A method for pulp preconditioning to impair strong nib acidification during cocoa fermentation in Malaysia. J Sci Food Agric 51: 35–45. https://doi.org/10.1002/jsfa.2740510105 doi: 10.1002/jsfa.2740510105
    [4] Afoakwa EO, Quao J, Budu AS, et al. (2012) Influence of pulp-preconditioning and fermentation on fermentative quality and appearance of Ghanaian cocoa (Theobroma cacao) beans. Int Food Res J 19: 127–133. https://docplayer.net/24269032
    [5] Hinneh M, Abotsi EE, Van de Walle D, et al. (2020) Pod storage with roasting: A tool to diversifying the flavor profiles of dark chocolates produced from 'bulk' cocoa beans? (Part Ⅱ: Quality and sensory profiling of chocolates). Food Res Int 132: 109–116. https://doi.org/10.1016/j.foodres.2020.109116 doi: 10.1016/j.foodres.2020.109116
    [6] Hanif N, Langkong J, Syarifuddin A (2020) Characterization of chemical and volatile compounds in cocoa beans (Theobroma cacao) from highland and lowland areas of Bantaeng, South Sulawesi. Canrea J: Food Technol, Nutr, and Culinary J 3: 94–103. https://doi.org/10.20956/canrea.v3i2.332 doi: 10.20956/canrea.v3i2.332
    [7] Meyer B, Biehl B, Said M, et al. (1989) Post‐harvest pod storage: A method for pulp preconditioning to impair strong nib acidification during cocoa fermentation in Malaysia. J Sci Food Agric 48: 285–304. https://doi.org/10.1002/jsfa.2740480305 doi: 10.1002/jsfa.2740480305
    [8] Sukendar NK, Tawali AB, Salengke S, et al. (2019) Changes in physical-chemical properties during the fresh cocoa fermentation process. Canrea J: Food Technol, Nutr, Culinary J 2: 98–105. https://doi.org/10.20956/canrea.v2i2.214 doi: 10.20956/canrea.v2i2.214
    [9] Association of Official Analytical Chemists (AOAC) (2016) Official methods of analysis. Available from: https://www.yumpu.com/en/document/read/65659513/free-download-pdf-official-methods-of-analysis-of-aoac-international-20th-aoac-2016-gigapaper.
    [10] Association of Official Analytical Chemists (AOAC) (2005) Official methods of analysis. Available from: https://www.researchgate.net/publication/292783651_AOAC_2005.
    [11] Christova-Bagdassrian VL, Chohadjieva D, Atanassova M (2014) Total phenolics and total flavonoids, nitrate contents and microbiological tests in dry extract of Bulgarian white birch leaves (Betula pendula). Int J Adv Res 2: 668–674. http://www.journalijar.com
    [12] Marrubini G, Papetti A, Genorini E, et al. (2017) Determination of the sugar content in commercial plant milks by near infrared spectroscopy and Luff-choorl total glucose titration. Food Anal Methods 10: 1556–1567. https://doi.org/10.1007/s12161-016-0713-1 doi: 10.1007/s12161-016-0713-1
    [13] Starch Institut International (2002) Denmark International Standard: ISO 5377ISI 28-Le Determination of Reducing Sugar, DE by Luff Schoorl's Method. Science Park Aarhus, Copenhagen. http://www.starch.dk/isi/methods/28luff.htm
    [14] Pradnyana IKDA, Parwata IMOA, Sudarma N (2014) Determination of sucrose in the coconut sap and sugar palm sap using Luff-Schoorl method. J Chem Lab 1: 33–37. http://erepo.unud.ac.id/id/eprint/749/1/c70ef0dfe3f16640de4a209044739cd8.pdf
    [15] Meersman E, Struyf N, Kyomugasho C, et al. (2017) Characterization and degradation of pectic polysaccharides in cocoa pulp. J Agric Food Chem 65: 9726–9734. https://doi.org/10.1021/acs.jafc.7b03854 doi: 10.1021/acs.jafc.7b03854
    [16] Afoakwa EO (2016) Chocolate Science and Technology, 2nd Ed., John Weley & Sons.Ltd, United Kingdom. Available from: https://zeabooks.com/book/chocolate-science-and-technology-2nd-edition/#download.
    [17] Nunes CSO, Da Silva MLC, Camilloto GP, et al. (2020) Potential applicability of cocoa pulp (Theobroma cacao L.) as an adjunct for beer production. Sci World J 2020: 3192585. https://doi.org/10.1155/2020/3192585 doi: 10.1155/2020/3192585
    [18] Aroyeun SO, Ogunbayo JO, Olaiya AO (2006) Effect of modified packaging and storage time of cocoa pods on the commercial quality of cocoa beans. Br Food J 108: 141–151. https://doi.org/10.1108/00070700610644951 doi: 10.1108/00070700610644951
    [19] Biehl B, Meyer B, Crone G, et al. (1989) Chemical and physical changes in the pulp during ripening and post‐harvest storage of cocoa pods. J Sci Food Agric 48: 189–208. https://doi.org/10.1002/jsfa.2740480207 doi: 10.1002/jsfa.2740480207
    [20] Hinneh M, Semanhyia E, Van de Walle D, et al. (2018) Assessing the influence of pod storage on sugar and free amino acid profiles and the implications on some Maillard reaction related flavor volatiles in Forastero cocoa beans. Food Res Int 111: 607–620. https://doi.org/10.1016/j.foodres.2018.05.064 doi: 10.1016/j.foodres.2018.05.064
    [21] Kou X, Feng Y, Yuan S, et al. (2021) Different regulatory mechanisms of plant hormones in the ripening of climacteric and non-climacteric fruits: A review. Plant Mol Biol 107: 477–497. https://doi.org/10.1007/s11103-021-01199-9 doi: 10.1007/s11103-021-01199-9
    [22] Amoa-Awua W, Madsen MTJ (2006) Quality manual for production and primary processing of cocoa. Council for Scientific and Industrial Research (CSIR), Food Research Institute, Ghana. https://csirspace.foodresearchgh.site/handle/123456789/1248
    [23] Afoakwa EO, Paterson A, Fowler M, et al. (2008) Modelling tempering behaviour of dark chocolates from varying particle size distribution and fat content using response surface methodology. Innovative Food Sci Emerging Technol 9: 527–533. https://doi.org/10.1016/j.ifset.2008.02.002. doi: 10.1016/j.ifset.2008.02.002
    [24] Pettipher GL (1986) Analysis of cocoa pulp and the formulation of a standardised artificial cocoa pulp medium. J Sci Food Agric 37: 297–309. https://doi.org/10.1002/jsfa.2740370315 doi: 10.1002/jsfa.2740370315
    [25] Cooper JM (2006) 7-Sucrose. In: Spillane WJ (Ed.), Optimising Sweet Taste in Foods—A volume in Woodhead Publishing Series in Food Science, Technology and Nutrition, Woodhead Publishing Limited, 135–152. https://doi.org/10.1533/9781845691646.2.135
    [26] Kader AA, Holcroft DM (2018) Postharvest: An introduction to the physiology and handling of fruit, vegetables and ornamentals. HortTechnology 9: 299. https://doi.org/10.21273/horttech.9.2.299 doi: 10.21273/horttech.9.2.299
    [27] Sakiroh, Taryono, Setyastuti P (2018) Dynamics of storage materials in cotyledon during cocoa seed germination. Agric Sci 3: 12–20. https://doi.org/10.22146/ipas.34594 doi: 10.22146/ipas.34594
    [28] Kim H, Keeney PG (1983) Method of analysis for (‐)‐epicatechin in cocoa beans by high performance liquid chromatography. J Food Sci 48: 548–551. https://doi.org/10.1111/j.1365-2621.1983.tb10787.x doi: 10.1111/j.1365-2621.1983.tb10787.x
    [29] Serra Bonvehí J, Ventura Coll F (1997) Evaluation of bitterness and astringency of polyphenolic compounds in cocoa powder. Food Chem 60: 365–370. https://doi.org/10.1016/S0308-8146(96)00353-6 doi: 10.1016/S0308-8146(96)00353-6
  • Reader Comments
  • © 2023 the Author(s), licensee AIMS Press. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0)
通讯作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

  1. 本站搜索
  2. 百度学术搜索
  3. 万方数据库搜索
  4. CNKI搜索

Metrics

Article views(1168) PDF downloads(205) Cited by(0)

Article outline

Figures and Tables

Figures(10)  /  Tables(6)

/

DownLoad:  Full-Size Img  PowerPoint
Return
Return

Catalog