Search Advanced

AIMS Microbiology

2019, Volume 5, Issue 3: 272-284. doi: 10.3934/microbiol.2019.3.272
Previous Article Next Article
Research article

Acinetobacter baumannii in sheep, goat, and camel raw meat: virulence and antibiotic resistance pattern

  • 1.
    Ph.D Student of Microbiology, Department of Microbiology, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
  • 2.
    Department of Microbiology, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
  • Received: 13 May 2019 Accepted: 27 August 2019 Published: 23 September 2019

  • Acinetobacter genus belongs to a group of Gram-negative coccobacillus. These bacteria are isolated from human and animal origins. Antimicrobial agents play a vital role in treating infectious diseases in both humans and animals, and Acinetobacter in this regard is defined as an organism of low virulence. The current study aimed to evaluate antibiotic resistance properties and virulence factor genes in Acinetobacter baumannii strains isolated from raw animal meat samples. Fresh meat samples from 124 sheep, 162 goat, and 95 camels were randomly collected from Isfahan and Shahrekord cities in Iran. Most A. baumannii strains isolated from sheep meat samples represented fimH (82.35%), aac(3)-IV (78.43%), sul1 (78.43%) and Integron Class I (96.07%) genes. Moreover, more than 50% of A. baumannii strains isolated from sheep samples were resistant to streptomycin (54.90%), gentamycin (74.50%), co-trimoxazole (70.58%), tetracycline (82.35%), and trimethoprim (62.74%). Current findings revealed significant association between the presence of fimH, cnfI, afa/draBC, dfrA1, sulI, aac(3)-IV genes in sheep samples. Furthermore, significant association was observed between fimH, cnfI, sfa/focDE and dfrA1genes in goat meat samples. In sheep meat samples, significant differences were identified in resistance to gentamicin, tetracycline, and co-trimoxazole in comparison with other antibiotics. Finally, there were statistically significant differences between the incidences of resistance to gentamicin, tetracycline, and co-trimoxazole in comparison with other antibiotics in all strains. In conclusion, the presence of virulence factors and antibiotic resistance in A. baumannii strains isolated from animal meat samples showed that animals should be considered as a potential reservoir of multidrug-resistant A. baumannii.

    Citation: Neda Askari, Hassan Momtaz, Elahe Tajbakhsh. Acinetobacter baumannii in sheep, goat, and camel raw meat: virulence and antibiotic resistance pattern[J]. AIMS Microbiology, 2019, 5(3): 272-284. doi: 10.3934/microbiol.2019.3.272

    Related Papers:

  • Acinetobacter genus belongs to a group of Gram-negative coccobacillus. These bacteria are isolated from human and animal origins. Antimicrobial agents play a vital role in treating infectious diseases in both humans and animals, and Acinetobacter in this regard is defined as an organism of low virulence. The current study aimed to evaluate antibiotic resistance properties and virulence factor genes in Acinetobacter baumannii strains isolated from raw animal meat samples. Fresh meat samples from 124 sheep, 162 goat, and 95 camels were randomly collected from Isfahan and Shahrekord cities in Iran. Most A. baumannii strains isolated from sheep meat samples represented fimH (82.35%), aac(3)-IV (78.43%), sul1 (78.43%) and Integron Class I (96.07%) genes. Moreover, more than 50% of A. baumannii strains isolated from sheep samples were resistant to streptomycin (54.90%), gentamycin (74.50%), co-trimoxazole (70.58%), tetracycline (82.35%), and trimethoprim (62.74%). Current findings revealed significant association between the presence of fimH, cnfI, afa/draBC, dfrA1, sulI, aac(3)-IV genes in sheep samples. Furthermore, significant association was observed between fimH, cnfI, sfa/focDE and dfrA1genes in goat meat samples. In sheep meat samples, significant differences were identified in resistance to gentamicin, tetracycline, and co-trimoxazole in comparison with other antibiotics. Finally, there were statistically significant differences between the incidences of resistance to gentamicin, tetracycline, and co-trimoxazole in comparison with other antibiotics in all strains. In conclusion, the presence of virulence factors and antibiotic resistance in A. baumannii strains isolated from animal meat samples showed that animals should be considered as a potential reservoir of multidrug-resistant A. baumannii.


    加载中

    Acknowledgments


    The authors would like to thank Dr. Abbas Farahani for his assistance in sample collection and PCR genetic alignments. This work was financially supported by the Islamic Azad University, Shahrekord Branch, Shahrekord, Iran.

    Conflict of interest


    The authors declare that they have no competing interests.

    [1] Huang L, Sun L, Yan Y (2013) Clonal spread of carbapenem resistant Acinetobacter baumannii ST92 in a Chinese Hospital during a 6-year period. J Microbiol 51: 113–117. doi: 10.1007/s12275-013-2341-4
    [2] Almasaudi SB (2018) Acinetobacter spp. As nosocomial pathogens: Epidemiology and resistance features. Saudi J Biol Sci 25: 586–596.
    [3] Peleg AY, Seifert H, Paterson DL (2008) Acinetobacter baumannii: emergence of a successful pathogen. Clin Microbiol Rev 21: 538–582. doi: 10.1128/CMR.00058-07
    [4] Dahiru M, Enabulele O (2015) Acinetobacter baumannii in birds' feces: A public health threat to vegetables and irrigation farmers. Adv Microbiol 5: 693–698. doi: 10.4236/aim.2015.510072
    [5] Guardabassi L, Schwarz S, Lloyd DH (2004) Pet animals as reservoirs of antimicrobial-resistant bacteria. J Antimicrob Chemother 54: 321–332. doi: 10.1093/jac/dkh332
    [6] Jung J, Park W (2015) Acinetobacter species as model microorganisms in environmental microbiology: current state and perspectives. Appl Microbiol Biotechnol 99: 2533–2548. doi: 10.1007/s00253-015-6439-y
    [7] APIC guide (2010) Guide to the elimination of multidrug-resistant Acinetobacter baumannii transmission in healthcare settings. Washington, DC: APIC Headquarters. Available from: https://apic.org/Resource_/EliminationGuideForm/b8b0b11f-1808-4615-890b-f652d116ba56/File/APIC-AB-Guide.pdf
    [8] Kurcik-Trajkovska B (2009) Acinetobacter spp.-A serious enemy threatening hospitals worldwide. Macedonian J Med Sci 2: 157–162.
    [9] Barnhart MM, Chapman MR (2006) Curli biogenesis and function. Annu Rev Microbiol 60: 131–147. doi: 10.1146/annurev.micro.60.080805.142106
    [10] Müller S, Janssen T, Wieler LH (2014) Multidrug resistant Acinetobacter baumannii in veterinary medicine-emergence of an underestimated pathogen? Berl Munch Tierarztl Wochenschr 127: 435–446.
    [11] Vaneechoutte M, Devriese LA, Dijkshoorn L, et al. (2000) Acinetobacter baumannii-infected vascular catheters collected from horses in an equine clinic. J Clin Microbiol 38: 4280–4281.
    [12] Francey T, Gaschen F, Nicolet J, et al. (2000) The role of Acinetobacter baumannii as a nosocomial pathogen for dogs and cats in an intensive care unit. J Vet Intern Med 14: 177–183. doi: 10.1111/j.1939-1676.2000.tb02233.x
    [13] Espinal P, Seifert H, Dijkshoorn L, et al. (2012) Rapid and accurate identification of genomic species from the Acinetobacter baumannii (Ab) group by MALDI-TOF MS. Clin Microbiol Infect 18: 1097 –1103. doi: 10.1111/j.1469-0691.2011.03696.x
    [14] Momtaz H, Seifati SM, Tavakol M (2015) Determining the prevalence and detection of the most prevalent virulence genes in Acinetobacter baumannii isolated from hospital infections. Int J Med Lab 2: 87–97.
    [15] Tavakol M, Momtaz H, Mohajeri P, et al. (2018) Genotyping and distribution of putative virulence factors and antibiotic resistance genes of Acinetobacter baumannii strains isolated from raw meat. Antimicrob Resist Infect Control 7: 120. doi: 10.1186/s13756-018-0405-2
    [16] CLSI (2017) Performance standards for antimicrobial susceptibility testing; twenty-fifth informational supplement. Wayne: Clinical and Laboratory Standards Institute.
    [17] Kiani S, Momtaz H, Serajian AA, et al. (2016) Detection of integrons in Acinetobacter baumannii strains isolated from the nosocomial infections of Ahvaz city and their relation with the resistance pattern. Int J Med Lab 3: 50–63.
    [18] Endimiani A, Hujer KM, Hujer AM, et al. (2011) Acinetobacter baumannii isolates from pets and horses in Switzerland: molecular characterization and clinical data. J Antimicrob Chemother 66: 2248–2254. doi: 10.1093/jac/dkr289
    [19] Al Atrouni A, Joly-Guillou ML, Hamze M, et al. (2016) Reservoirs of non-baumannii acinetobacter species. Front Microbiol 7: 49.
    [20] Hamouda A, Vali L, Amyes SG (2008) Gram-negative non-fermenting bacteria from food-producing animals are low risk for hospital-acquired infections. J Chemother 20: 702–708. doi: 10.1179/joc.2008.20.6.702
    [21] Hamouda A, Findlay J, Al Hassan L, et al. (2011) Epidemiology of Acinetobacter baumannii of animal origin. Int J Antimicrob Agents 38: 314–318. doi: 10.1016/j.ijantimicag.2011.06.007
    [22] Rafei R, Hamze M, Pailhoriès H, et al. (2015) Extrahuman epidemiology of Acinetobacter baumannii in Lebanon. Appl Environ Microbiol 81: 2359–2367. doi: 10.1128/AEM.03824-14
    [23] Nam HM, Lim SK, Kim JM, et al. (2010) In vitro activities of antimicrobials against six important species of gram-negative bacteria isolated from raw milk samples in Korea. Foodborne Pathog Dis 7: 221–224. doi: 10.1089/fpd.2009.0406
    [24] Gurung M, Nam HM, Tamang MD, et al. (2013) Prevalence and antimicrobial susceptibility of Acinetobacter from raw bulk tank milk in Korea. J Dairy Sci 96: 1997–2002. doi: 10.3168/jds.2012-5965
  • Reader Comments
  • © 2019 the Author(s), licensee AIMS Press. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0)
通讯作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

  1. 本站搜索
  2. 百度学术搜索
  3. 万方数据库搜索
  4. CNKI搜索
AIMS Microbiology
2.7 7.0

Metrics

Article views(4509) PDF downloads(613) Cited by(18)

Preview PDF
Download XML
Export Citation
Article outline

Figures and Tables

Tables(8)

Other Articles By Authors

Related pages

Tools

/

DownLoad:  Full-Size Img  PowerPoint
Return
Return

Catalog