Isolation  and  characterization  of  the  <em>Staphylococcus  aureus</em> bacteriophage vB_SauS_SA2

Jia Wang; Feiyang Zhao; Huzhi Sun; Qian Wang; Can Zhang; Wenhua Liu; Ling Zou; Qiang Pan; Huiying Ren; Jia Wang; Feiyang Zhao; Huzhi Sun; Qian Wang; Can Zhang; Wenhua Liu; Ling Zou; Qiang Pan; Huiying Ren

doi:10.3934/microbiol.2019.3.285

AIMS Microbiology

2019, Volume 5, Issue 3: 285-307. doi: 10.3934/microbiol.2019.3.285

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Research article

Isolation and characterization of the Staphylococcus aureus bacteriophage vB_SauS_SA2

1.
Qingdao Agricultural University, College of Veterinary Medicine, Shandong 266109, China
2.
Qingdao Phagepharm Bio-tech Co, Ltd, Shandong 266109, China

Received: 30 June 2019 Accepted: 25 September 2019 Published: 27 September 2019

A novel bacteriophage vB_SauS_SA2 (hereafter designated SA2) that infects Staphylococcus aureus was isolated. At a multiplicity of infection (MOI) of 0.1, phage SA2 had a latent period of about 10 min with a burst size of 293 PFUs/infected cell (PFU, plaque forming unit). Phage SA2 had a double-stranded DNA genome with a length of 89,055 bp and a G + C content of 31.9%. The genome contained 130 open reading frames (ORFs), 28 of which had assigned functions, and 18 were unique. One tRNA gene (tRNA^Asn) was discovered, and no virulence genes were identified. Its genome showed very low similarity with phage genomes deposited in public databases (75% nucleotide identity and 7% query coverage). The unique characteristics of phage SA2 led to the proposal of a new Siphoviridae genus named ‘SA2likevirus’.
- Staphylococcus aureus,
- Bacteriophage vB_SauS_SA2,
- Genome analysis
Citation: Jia Wang, Feiyang Zhao, Huzhi Sun, Qian Wang, Can Zhang, Wenhua Liu, Ling Zou, Qiang Pan, Huiying Ren. Isolation and characterization of the Staphylococcus aureus bacteriophage vB_SauS_SA2[J]. AIMS Microbiology, 2019, 5(3): 285-307. doi: 10.3934/microbiol.2019.3.285

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Abstract

A novel bacteriophage vB_SauS_SA2 (hereafter designated SA2) that infects Staphylococcus aureus was isolated. At a multiplicity of infection (MOI) of 0.1, phage SA2 had a latent period of about 10 min with a burst size of 293 PFUs/infected cell (PFU, plaque forming unit). Phage SA2 had a double-stranded DNA genome with a length of 89,055 bp and a G + C content of 31.9%. The genome contained 130 open reading frames (ORFs), 28 of which had assigned functions, and 18 were unique. One tRNA gene (tRNA^Asn) was discovered, and no virulence genes were identified. Its genome showed very low similarity with phage genomes deposited in public databases (75% nucleotide identity and 7% query coverage). The unique characteristics of phage SA2 led to the proposal of a new Siphoviridae genus named ‘SA2likevirus’.

Acknowledgments

This work was supported by a grant from the Donkey Industry Innovation Team Program of Modern Agricultural Technology System from Shandong Province, China (SDAIT-27).

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

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