Optimization of factors influencing exopolysaccharide production by <em>Halomonas xianhensis</em> SUR308 under batch culture

Jhuma Biswas; Amal K. Paul; Jhuma Biswas; Amal K. Paul

doi:10.3934/microbiol.2017.3.564

AIMS Microbiology

2017, Volume 3, Issue 3: 564-579. doi: 10.3934/microbiol.2017.3.564

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Research article

Optimization of factors influencing exopolysaccharide production by Halomonas xianhensis SUR308 under batch culture

Jhuma Biswas ,
Amal K. Paul ^,

Microbiology Laboratory, Department of Botany, University of Calcutta, Kolkata-700019, West Bengal, India

Received: 19 April 2017 Accepted: 28 June 2017 Published: 06 July 2017

A moderately halophilic bacterium, Halomonas xianhensis SUR308 (GenBank Accession No. KJ933394) was isolated from multi-pond solar salterns of Odisha, India. Exopolysaccharide (EPS) production by this strain in malt extract yeast extract (MY) medium has been optimized under batch culture system. Among the different media tested, MY medium showed an EPS production of 2.55 g/L, which increased to 2.85 g/L under optimized aeration. An initial pH of 7.5 and incubation temperature of 32 °C were found to be most suitable for EPS production by the isolate under aerobic condition. An EPS production of 3.85 g/L was achieved when the growth medium was supplemented with 2.5% NaCl. Glucose was the most favourable carbon source for EPS production and maximum production (5.70 g/L) was recorded with 3% glucose. However, growth as well as production of EPS was remarkably affected when the growth medium was supplemented with hydrocarbons as sole source of carbon. Among different nitrogen sources, casein hydrolysate at 0.5% level was proved to be the best for EPS production and an initial inoculum dose of 7% (v/v) enhanced the EPS production to 7.78 g/L, while the divalent metal ions were in general toxic to growth and EPS production, EPS synthesis by SUR308 was enhanced with Cr (VI) supplementation.
- Halomonas xianhensis,
- solar salterns,
- moderately halophilic bacterium,
- exopolysaccharides,
- hydrocarbons
Citation: Jhuma Biswas, Amal K. Paul. Optimization of factors influencing exopolysaccharide production by Halomonas xianhensis SUR308 under batch culture[J]. AIMS Microbiology, 2017, 3(3): 564-579. doi: 10.3934/microbiol.2017.3.564

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Abstract

A moderately halophilic bacterium, Halomonas xianhensis SUR308 (GenBank Accession No. KJ933394) was isolated from multi-pond solar salterns of Odisha, India. Exopolysaccharide (EPS) production by this strain in malt extract yeast extract (MY) medium has been optimized under batch culture system. Among the different media tested, MY medium showed an EPS production of 2.55 g/L, which increased to 2.85 g/L under optimized aeration. An initial pH of 7.5 and incubation temperature of 32 °C were found to be most suitable for EPS production by the isolate under aerobic condition. An EPS production of 3.85 g/L was achieved when the growth medium was supplemented with 2.5% NaCl. Glucose was the most favourable carbon source for EPS production and maximum production (5.70 g/L) was recorded with 3% glucose. However, growth as well as production of EPS was remarkably affected when the growth medium was supplemented with hydrocarbons as sole source of carbon. Among different nitrogen sources, casein hydrolysate at 0.5% level was proved to be the best for EPS production and an initial inoculum dose of 7% (v/v) enhanced the EPS production to 7.78 g/L, while the divalent metal ions were in general toxic to growth and EPS production, EPS synthesis by SUR308 was enhanced with Cr (VI) supplementation.

References

[1]	Vandamme EJ, De Baets S, Steinbuchel A (2002) Biopolymers, Polysaccharides I: Polysaccharides from Prokaryotes, Wenham: Wiley-VCH.
[2]	Wan-Mohtar WAAQ, Young L, Abbott GM, et al. (2016) Antimicrobial properties and cytotoxicity of sulfated (1,3)-β-D-Glucan from the Mycelium of the mushroom Ganodermalucidum. J Microbiol Biotechnol 26: 999–1010. doi: 10.4014/jmb.1510.10018
[3]	Margesin R, Schinner F (2001) Potential of halotolerant and halophilic microorganisms for biotechnology. Extremophiles 5: 73–83. doi: 10.1007/s007920100184
[4]	Quesada E, Bejar V, Calvo C (1993) Exopolysaccharide production by Volcaniella eurihalina. Experientia 49: 1037–1041.
[5]	Bouchotroch S, Quesada E, del Moral A, et al. (2001) Halomonas maura sp. nov., a novel moderately halophilic, exopolysaccharide-producing bacterium. Int J Syst Evol Microbiol 51: 1625–1632.
[6]	Calvo C, Ferrer MR, Martinez-Checa F, et al. (1995) Some rheological properties of the extracellular polysaccharide produced by Volcaniella eurihalina F2-7. Appl Biochem Biotechnol 55: 45–54. doi: 10.1007/BF02788747
[7]	Calvo C, Martinez-Checa F, Mota A, et al. (1998) Effect of cations, pH and sulfate content on the viscosity and emulsifying activity of the Halomonas eurihalina exopolysaccharide. J Ind Microbiol Biotechnol 20: 205–209.
[8]	Martinez-Checa F, Bejar V, Martinez-Canovas MJ, et al. (2005) Halomonas almeriensis sp. nov., a moderately halophilic, exopolysaccharide-producing bacterium from Cabo de Gata, Almeria, south-east Spain. Int J Syst Evol Microbiol 55: 2007–2011.
[9]	Mata JA, Bejar V, Llamas I, et al. (2006) EPS produced by the recently described halophilic bacteria Halomonas ventosae and Halomonas anticariensis. Res Microbiol 157: 827–835. doi: 10.1016/j.resmic.2006.06.004
[10]	Amjres H, Bejar V, Quesada E, et al. (2011) Halomonas rifensis sp. nov., an exopolysaccharide-producing, halophilic bacterium isolated from a solar saltern. Int J Syst Evol Microbiol 61: 2600–2605.
[11]	Llamas I, Bejar V, Martinez-Checa F, et al. (2011) Halomonas stenophila sp. nov., a halophilic bacterium that produces sulphate exopolysaccharides with biological activity. Int J Syst Evol Microbiol 61: 2508–2514.
[12]	Biswas J, Mandal S, Paul AK (2015) Production, partial purification and some bio-physicochemical properties of EPS produced by Halomonas xianhensis SUR308 isolated from a saltern environment. J Biol Active Pdts Nat 5: 108–119.
[13]	Biswas J, Ganguly J, Paul AK (2015) Partial characterization of an extracellular polysaccharide produced by a moderately halophilic bacterium Halomonas xianhensis SUR308. Biofouling 31: 735–744.
[14]	Ventosa A, Quesada E, Rodriguez-Valera F, et al. (1982) Numerical taxonomy of moderately halophilic gram-negative rods. J Gen Microbiol 128: 1959–1986.
[15]	Dubois M, Gilles KA, Hamilton JK, et al. (1956) Colorimetric method for determination of sugars and related substances. Anal Chem 28: 350–356. doi: 10.1021/ac60111a017
[16]	Miller GL (1972) Use of DNS reagent for the determination of glucose. Anal Chem 31: 426–428.
[17]	Bejar V, Calvo C, Moliz J, et al. (1996) Effect of growth conditions on the rheological properties and chemical composition of Volcaniella eurihalina exopolysaccharide. Appl Biochem Biotechnol 59: 77–86. doi: 10.1007/BF02787859
[18]	Cerning J, Renard CMGC, Thibault JF, et al. (1994) Carbon source requirements for exopolysaccharide production by Lactobacillus casei CG11 and partial structure analysis of the polymer. Appl Environ Microbiol 60: 3914–3919.
[19]	Rehm BHA (2009) Microbial production of biopolymers and polymer precursors: applications and perspectives, Caister Academic Press.
[20]	Arias S, del Moral A, Ferrer MR, et al. (2003) Mauran, an exopolysaccharide produced by the halophilic bacterium Halomonas maura, with a novel composition and interesting properties for biotechnology. Extremophiles 7: 319–326. doi: 10.1007/s00792-003-0325-8
[21]	Martinez-Checa F, Toledo FL, El Mabrouki K, et al. (2007) Characteristics of bioemulsifier V2-7 synthesized in culture media added of hydrocarbons: chemical composition, emulsifying activity and rheological properties. Bioresource Technol 98: 3130–3135. doi: 10.1016/j.biortech.2006.10.026
[22]	Farres J, Caminal G, Lopez-Santin J (1997) Influence of phosphate on rhamnose-containing exopolysaccharide rheology and production by Klebsiella I-174. Appl Microbiol Biotechnol 48: 522–527. doi: 10.1007/s002530051090
[23]	Abe K, Hayashi H, Maloney PC (1996) Exchange of aspartate and alanine mechanism for development of a proton-motive force in bacteria. J Biol Chem 271: 3079–3084. doi: 10.1074/jbc.271.6.3079
[24]	Kim SY, Kim JH, Kim CJ, et al. (1996) Metal adsorption of the polysaccharide produced from Methylobacterium organophilum. Biotechnol Lett 18: 1161–1164. doi: 10.1007/BF00128585
[25]	Kazy SK, Sar P, Singh SP, et al. (2002) Extracellular polysaccharides of a copper-sensitive and a copper-resistant Pseudomonas aeruginosa strain: synthesis, chemical nature and copper binding. Word J Microbiol Biotechnol 18: 583–588.
[26]	Loaec M, Olier R, Guezennec J (1998) Chelating properties of bacterial polysaccharides from deep-sea hydrothermal vents. Carbohyd Polym 35: 65–70. doi: 10.1016/S0144-8617(97)00109-4
[27]	Hassen N, Saidi M, Cherif M (1998) Effects of heavy metals on Pseudomonas aeruginosa and Bacillus thuringiensis. Bioresource Technol 68: 73–82.
[28]	Poli A, Moriello VS, Esposito E, et al. (2004) Exopolysaccharide production by a new Halomonas strain CRSS isolated from saline lake Cape Russell in Antarctica growing on complex and defined media. Biotechnol Lett 26: 1635–1643. doi: 10.1007/s10529-004-3187-y
[29]	Romano I, Lama L, Nicolaus B, et al. (2006) Oceanobacillus oncorhynchi subsp. Incaldanensis subsp. nov., an alkalitolerant halophile isolated from an algal mat collected from a sulfurous spring in Campania (Italy), and emended description of Oceanobacillus oncorhynchi. Int J Syst Evol Microbiol 56: 805–810.
[30]	Llamas I, Amjres H, Mata JA, et al. (2012) The potential biotechnological applications of the exopolysaccharide produced by the halophilic bacterium Halomonas almeriensis. Molecules 17: 7103–7120. doi: 10.3390/molecules17067103
[31]	Poli A, Nicolaus B, Denizci AA, et al. (2013) Halomonas smyrnensis sp. nov., a moderately halophilic, exopolysaccharide-producing bacterium. Int J Syst Evol Microbiol 63: 10–18.
[32]	Amjres H, Bejar V, Quesada E, et al. (2015) Characterization of haloglycan, an exopolysaccharide produced by Halomonas stenophila HK30. Int J Biol Macromol 72: 117–124.
[33]	Poli A, Kazak H, Gurleyendag B, et al. (2009) High level synthesis of levan by a novel Halomonas species growing on defined media. Carbohyd Polym 78: 651–657. doi: 10.1016/j.carbpol.2009.05.031

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