Selective adhesion of wastewater bacteria to Pleurotus ostreatus mycelium in a trickle-bed bioreactor

Kateřina Svobodová; Denisa Petráčková; Hana Szabad; Čeněk Novotný; Kateřina Svobodová; Denisa Petráčková; Hana Szabad; Čeněk Novotný

doi:10.3934/environsci.2016.3.395

AIMS Environmental Science

2016, Volume 3, Issue 3: 395-407. doi: 10.3934/environsci.2016.3.395

Previous Article Next Article

Research article Special Issues

Selective adhesion of wastewater bacteria to Pleurotus ostreatus mycelium in a trickle-bed bioreactor

1.
Laboratory of Environmental Biotechnology, Institute of Microbiology of the CAS, v.v.i., Vídeňská 1083, 14220 Prague, Czech Republic
2.
Laboratory of Cell Signalization, Institute of Microbiology of the CAS, v.v.i., Vídeňská 1083, 14220 Prague, Czech Republic

Received: 03 May 2016 Accepted: 12 July 2016 Published: 15 July 2016

The work is focused on spontaneous colonization of fungal mycelium by invading microorganisms in a trickle-bed fungal bioreactor operating under semi-sterile conditions. Pleurotus ostreatus was grown under the flow of synthetic wastewater containing activated sludge bacteria and the microbial consortium developed in the reactor was characterized. Genotype and phenotype profile of the reactor-invading, bacterial consortium was clearly distinctive from that of the original activated sludge. The bacterial consortium from the reactor contained a higher portion of bacteria capable of cellobiose utilization and a small amount of bacteria with the ability to utilize benzoic acids. The invading bacteria had no effect on the dye decolorization performance of the fungal reactor. Five bacterial strains colonizing P. ostreatus reactor cultures were isolated and identified as species of the genera Pseudomonas and Bacillus. Except for Bacillus cereus all strains displayed a potential to inhibit fungal growth on solid media (14 to 51 % inhibition) which was comparable or higher than that of the reference bacterial strains. The pH- and media composition-dependence of the growth inhibition was demonstrated.
- Pleurotus ostreatus,
- activated sludge,
- fungal/bacterial co-culture,
- trickle-bed bioreactor,
- dye decolorization capacity,
- microbial community structure,
- fungal growth inhibition
Citation: Kateřina Svobodová, Denisa Petráčková, Hana Szabad, Čeněk Novotný. Selective adhesion of wastewater bacteria to Pleurotus ostreatus mycelium in a trickle-bed bioreactor[J]. AIMS Environmental Science, 2016, 3(3): 395-407. doi: 10.3934/environsci.2016.3.395

Related Papers:

Abstract

The work is focused on spontaneous colonization of fungal mycelium by invading microorganisms in a trickle-bed fungal bioreactor operating under semi-sterile conditions. Pleurotus ostreatus was grown under the flow of synthetic wastewater containing activated sludge bacteria and the microbial consortium developed in the reactor was characterized. Genotype and phenotype profile of the reactor-invading, bacterial consortium was clearly distinctive from that of the original activated sludge. The bacterial consortium from the reactor contained a higher portion of bacteria capable of cellobiose utilization and a small amount of bacteria with the ability to utilize benzoic acids. The invading bacteria had no effect on the dye decolorization performance of the fungal reactor. Five bacterial strains colonizing P. ostreatus reactor cultures were isolated and identified as species of the genera Pseudomonas and Bacillus. Except for Bacillus cereus all strains displayed a potential to inhibit fungal growth on solid media (14 to 51 % inhibition) which was comparable or higher than that of the reference bacterial strains. The pH- and media composition-dependence of the growth inhibition was demonstrated.

References

[1]	Andleeb S, Atiq N, Robson GD, et al. (2012) An investigation of anthraquinone dye biodegradation by immobilized Aspergillus flavus in fluidized bed bioreactor. Env Sci Poll Res 19: 1728-1737. doi: 10.1007/s11356-011-0687-x
[2]	Rodriguez-Rodriguez CE, Baron E, Gago-Ferrero P, et al. (2012) Removal of pharmaceuticals, polybrominated flame retardants and UV-filters from sludge by the fungus Trametes versicolor in bioslurry reactor. J Haz Mat 233: 235-243.
[3]	Rosales E, Perez-Paz A, Vazquez X, et al. (2012) Isolation of novel benzo[a]anthracene-degrading microorganisms and continuous bioremediation in an expanded-bed bioreactor. Bioprocess Biosys Eng 35: 851-855. doi: 10.1007/s00449-011-0669-x
[4]	Yadav M, Srivastva N, Shukla AK, et al. (2015) Efficacy of Aspergillus sp. for degradation of chlorpyrifos in batch and continuous aerated packed bed bioreactors. Appl Biochem Biotech 175: 16-24.
[5]	Knapp JS, Vantoch-Wood EJ, Zhang F (2008) Use of wood-rotting fungi for the decolorization of dyes and industrial effluents, In: Gadd, G.M. (Ed.) Fungi in Bioremediation, Cambridge University Press, 242-304.
[6]	Cruz-Morato C, Ferrando-Climent L, Rodriguez-Mozaz S, et al. (2013) Degradation of pharmaceuticals in non-sterile urban wastewater by Trametes versicolor in a fluidized bed bioreactor. Water Res 47: 5200-5210. doi: 10.1016/j.watres.2013.06.007
[7]	Spina F, Romagnolo A, Prigione V, et al. (2014) A scaling-up issue: the optimal bioreactor configuration for effective fungal treatment of textile wastewaters. Chem Eng Trans 38: 37-42.
[8]	Gros M, Cruz-Morato C, Marco-Urrea E, et al. (2014) Biodegradation of the X-ray contrast agent iopromide and the fluoroquinolone antibiotic ofloxacin by the white rot fungus Trametes versicolor in hospital wastewaters and identification of degradation products. Water Res 60: 228-241. doi: 10.1016/j.watres.2014.04.042
[9]	Li X, de Toledo RA, Wang SP, et al. (2015) Removal of carbamazepine and naproxen by immobilized Phanerochaete chrysosporium under non-sterile condition. New Biotechnol 32: 282-289. doi: 10.1016/j.nbt.2015.01.003
[10]	Lu XM, Ma LH, Wang ZH, et al. (2010) Application of polymerase chain reaction-denaturing gradient gel electrophoresis to resolve taxonomic diversity in white rot fungus reactors. Env Eng Sci 27: 493-503. doi: 10.1089/ees.2010.0007
[11]	Hogan DA, Wargo MJ, Beck N, Bacterial biofilm on fungal surfaces, In: Kjelleberg S, Givskov M, eds., The Biofilm Mode of Life: Mechanisms and Adaptations, Horizon Scientific Press, 2007, 248.
[12]	Cho YS, Kim JS, Crowley DE, et al. (2003) Growth promotion of the edible fungus Pleurotus ostreatus by fluorescent pseudomonads. FEMS Microbiol Lett 218: 271-276. doi: 10.1016/S0378-1097(02)01144-8
[13]	Radtke C, Cook WS, Anderson A (1994) Factors affecting antagonism of the growth of Phanerochaete chrysosporium by bacteria isolated from soils. Appl Microbiol Biotechnol 41: 274-280. doi: 10.1007/BF00186972
[14]	Matsumura E, Yamamoto E, Numata A, et al. (1986) Structure of the laccase-catalyzed oxidation products of hydroxy-benzoic acids in the presence of ABTS (2,2´-azino-di-(3-ethylbenzothiazoline-6-sulfatic acid)). Agr Biol Chem 50: 1355-1357.
[15]	DeJong E, Cazemier AE, Field JA, et al. (1994) Physiological role of chlorinated aryl alcohols biosynthesized de-novo by the white-rot fungus Bjerkandera sp. strain Bos55. Appl Env Microbiol 60: 271-277.
[16]	Svobodová K, Majcherczyk A, Novotný Č, et al. (2008) Implication of mycelium-associated laccase from Irpex lacteus in decolorization of synthetic dyes. Biores Technol 99: 463-471. doi: 10.1016/j.biortech.2007.01.019
[17]	Altun O, Botero-Kleiven S, Carlsson S, et al. (2015) Rapid identification of bacteria from positive blood culture bottles by MALDI-TOS MS following short-term incubation on solid media. J Med Microbiol 64: 1346-1352. doi: 10.1099/jmm.0.000168
[18]	Garland JL, Mills AL (1991) Classification and characterization of heterotrophic microbial communities on the basis of patterns of community-level sole-carbon-source utilization. Appl Env Microbiol 57: 2351-2359.
[19]	Stach JEM, Bathe S, Clapp JP, et al. (2001) PCR-SSCP comparison of 16S rDNA sequence diversity in soil DNA obtained using different isolation and purification methods. FEMS Microbiol Ecol 36: 139-151. doi: 10.1111/j.1574-6941.2001.tb00834.x
[20]	Heuer H, Krsek M, Baker P, et al. (1997) Analysis of actinomycete communities by specific amplification of genes encoding 16S rRNA and gel-electrophoretic separation in denaturing gradients. Appl Env Microbiol 63: 3233-3241.
[21]	Prabhu MV, Karthikeyan R, Shanmugaprakash M (2016) Modeling and optimization by response surface methodology and neural network-genetic algorithm for decolorization of real textile dye effluent using Pleurotus ostreatus: a comparison study. Desal Wat Treatment 57: 13005-13019. doi: 10.1080/19443994.2015.1059372
[22]	Karas PA, Makri S, Papadopoulou ES, et al. (2016) The potential of organic substrates based on mushroom substrate and straw to dissipate fungicides contained in effluents from the fruit-packaging industry—Is there a role for Pleurotus ostreatus? Ecotoxicol Env Safety 124: 447-454. doi: 10.1016/j.ecoenv.2015.11.022
[23]	Skariyachan S, Prasanna A, Manjunath SP, et al. (2016) Environmental assessement of the degradation potential of mushroom fruit bodies of Pleurotus ostreatus (Jacq.:Fr.)P.Kumm. towards synthetic azo dyes and contaminating effluents collected from textile industries in Karnataka, India. Env Monitor Assessement 188: article nr. 121.
[24]	Svobodová K, Petráčková D, Kozická B, et al. (2016) Mutual interactions of Pleurotus ostreatus with bacteria of activated sludge in solid-bed bioreactors. World J Microbiol Biotechnol 32: 94.
[25]	Nakatsu CH (2007) Soil microbial community analysis using denaturing gradient gel electrophoresis. Soil Sci Soc Am J 71: 562-571. doi: 10.2136/sssaj2006.0080
[26]	Ruthes AC, Smiderle FR, Iacomini M (2016) Mushroom heteropolysaccharides: a review on their sources, structure and biological effects. Carbohydr Polymers 136: 358-375. doi: 10.1016/j.carbpol.2015.08.061
[27]	Deng YJ, Wang SY (2016) Synergistic growth in bacteria depends on substrate complexity. J Microbiol 54: 23-30. doi: 10.1007/s12275-016-5461-9
[28]	Baldrian P (2004) Increase of laccase activity during interspecific interactions of white-rot fungi. FEMS Microbiol Ecol 50: 245-253.
[29]	Kilani-Feki O, Ben Khedher S, Dammak M, et al. (2016) Improvement of antifungal metabolites production by Bacillus subtilis V26 for biocontrol of tomato postharvest disease. Biolog Control 95: 73-82.
[30]	Ligon JM, Hill DS, Hammer PE, et al. (2000) Natural products with antifungal activity from Pseudomonas biocontrol bacteria. Pest Management Sci 56: 688-695.
[31]	Pauliuc I, Botau D (2013) Antibacterial activity of Pleurotus ostreatus gemmotherapic extract. J Hortic Forest Biotech 17: 242-245.
[32]	Deng P, Wang XQ, Baird SM, et al. (2015) Complete genome of Pseudomonas chlororaphis strain UFB2, a soil bacterium with antibacterial activity against bacterial canker pathogen of tomato. Stand Gen Sci 10: article No. 117.
[33]	Etesami H, Alikhani HA (2016) Rhizosphere and endorhiza of oilseed rape (Brassica napus L.) plant harbor bacteria with multifaceted beneficials effects. Biol Control 94: 11-24.

Reader Comments

Your name:*

Email:*
© 2016 the Author(s), licensee AIMS Press. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0)