An alkali-tolerant strain <em>Microbacterium esteraromaticum</em> C26 produces a high yield of cation-independent bioflocculant

Weijie Liu; Rongnan He; Cong Liu; Weijie Liu; Rongnan He; Cong Liu

doi:10.3934/environsci.2016.3.408

AIMS Environmental Science

2016, Volume 3, Issue 3: 408-419. doi: 10.3934/environsci.2016.3.408

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Research article

An alkali-tolerant strain Microbacterium esteraromaticum C26 produces a high yield of cation-independent bioflocculant

School of Life Science, The Key Laboratory of Biotechnology for Medicinal Plant of Jiangsu Province, Jiangsu Normal University, Xuzhou 221116, Jiangsu Province, China

Received: 05 May 2016 Accepted: 02 July 2016 Published: 18 July 2016

A novel cation-independent bioflocculant BF-C26 produced by an alkali-tolerant strain Microbacterium esteraromaticum C26 was investigated in this study. The fermentation conditions for BF-C26 production were analyzed, including initial pH, carbon source, nitrogen source and the ratio of carbon source to nitrogen source. The result showed that 4.92 g/L bioflocculant BF-C26 was obtained at the following optimized conditions: 3 g/Lglucose as carbon source, 10 g/L peptone as nitrogen source and initial pH 8.1. The component assay showed that BF-C26 was a complex of 52.8% proteins and 28.1% polysaccharides. The effects of physicochemical parameters including metal ions, temperature, pH and bioflocculant dosage on flocculating properties of BF-C26 were optimized. The highest flocculating rate of 94.82% was achieved when 4 mg/L BF-C26 was added into the kaolin suspension at pH 8.23 and 30 °C. In this study, Microbacterium esteraromaticum was reported for the first time as a cation-independent bioflocculant producing strain.
- bioflocculant,
- cation-independent,
- Microbacterium esteraromaticum,
- polysaccharide
Citation: Weijie Liu, Rongnan He, Cong Liu. An alkali-tolerant strain Microbacterium esteraromaticum C26 produces a high yield of cation-independent bioflocculant[J]. AIMS Environmental Science, 2016, 3(3): 408-419. doi: 10.3934/environsci.2016.3.408

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Abstract

A novel cation-independent bioflocculant BF-C26 produced by an alkali-tolerant strain Microbacterium esteraromaticum C26 was investigated in this study. The fermentation conditions for BF-C26 production were analyzed, including initial pH, carbon source, nitrogen source and the ratio of carbon source to nitrogen source. The result showed that 4.92 g/L bioflocculant BF-C26 was obtained at the following optimized conditions: 3 g/Lglucose as carbon source, 10 g/L peptone as nitrogen source and initial pH 8.1. The component assay showed that BF-C26 was a complex of 52.8% proteins and 28.1% polysaccharides. The effects of physicochemical parameters including metal ions, temperature, pH and bioflocculant dosage on flocculating properties of BF-C26 were optimized. The highest flocculating rate of 94.82% was achieved when 4 mg/L BF-C26 was added into the kaolin suspension at pH 8.23 and 30 °C. In this study, Microbacterium esteraromaticum was reported for the first time as a cation-independent bioflocculant producing strain.

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