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AIMS Environmental Science

2015, Volume 2, Issue 3: 565-576. doi: 10.3934/environsci.2015.3.565
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Urinary DNA lesions as a biomarker for assessing male reproductive health

  • 1.
    School of Community and Environmental Health, College of Health Sciences, Old Dominion University, Norfolk, VA, USA;
  • 2.
    Institute of Labor, Occupational Safety and Health, Ministry of Labor, Taipei, Taiwan;
  • 3.
    Department of Occupational Safety and Health, Chung Shan Medical University, Taichung, Taiwan
  • Received: 26 March 2015 Accepted: 02 June 2015 Published: 15 June 2015

  • The study aimed to examine whether urinary 8-hydroxy-2'-deoxyguanosine (8-OHdG) could serve as a biomarker for assessing sperm DNA integrity. Urine and semen samples were collected from 124 coke-oven workers, who had chronically been exposed to polycyclic aromatic hydrocarbons (PAHs), from a steel plant in Kaohsiung, Taiwan. The coke-oven workers were divided into two subgroups: topside-oven workers and side-oven workers. Sperm DNA integrity was assessed in terms of DNA fragmentation and 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodGuo). Urine samples were used to detect 1-hydroxypyrene (1-OHP) and urinary 8-OHdG, which served to assess exposure to PAHs and the whole body oxidative stress, respectively. Urinary 1-OHP concentrations were significantly higher in the topside-oven workers than the side-oven workers (p = 0.02). Sperm 8-oxodGuo concentrations were correlated with percentages of sperm fragmentation (p = 0.044), but urinary 8-OHdG levels failed to correlate with sperm 8-oxodGuo levels and with percentages of fragmentation. In conclusion, exposure to PAHs was linked to increased oxidative stress on sperm. However, urinary 8-OHdG may not be a suitable biomarker for examining sperm DNA damage associated with oxidative stress.

    Citation: Hueiwang Anna Jeng, Chih-Hung Pan, Chao Mu-Rong. Urinary DNA lesions as a biomarker for assessing male reproductive health[J]. AIMS Environmental Science, 2015, 2(3): 565-576. doi: 10.3934/environsci.2015.3.565

    Related Papers:

  • The study aimed to examine whether urinary 8-hydroxy-2'-deoxyguanosine (8-OHdG) could serve as a biomarker for assessing sperm DNA integrity. Urine and semen samples were collected from 124 coke-oven workers, who had chronically been exposed to polycyclic aromatic hydrocarbons (PAHs), from a steel plant in Kaohsiung, Taiwan. The coke-oven workers were divided into two subgroups: topside-oven workers and side-oven workers. Sperm DNA integrity was assessed in terms of DNA fragmentation and 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodGuo). Urine samples were used to detect 1-hydroxypyrene (1-OHP) and urinary 8-OHdG, which served to assess exposure to PAHs and the whole body oxidative stress, respectively. Urinary 1-OHP concentrations were significantly higher in the topside-oven workers than the side-oven workers (p = 0.02). Sperm 8-oxodGuo concentrations were correlated with percentages of sperm fragmentation (p = 0.044), but urinary 8-OHdG levels failed to correlate with sperm 8-oxodGuo levels and with percentages of fragmentation. In conclusion, exposure to PAHs was linked to increased oxidative stress on sperm. However, urinary 8-OHdG may not be a suitable biomarker for examining sperm DNA damage associated with oxidative stress.


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    [1] IARC Monographs Polynuclear Aromatic Compounds, Part 1, Chemical, Environmental and Experimental Data. Vol. 32. Lyon, France: International Agency for Research on Cancer (1983).
    [2] Knuckles ME, Inyang F, Ramesh A. (2001) Acute and subchronic oral toxicities of benzo[a]pyrene in F-344 rats. Toxicol Sci 61: 382-388. doi: 10.1093/toxsci/61.2.382
    [3] Inyang F, Ramesh A, Kopsombut P, et al. (2003) Disruption of testicular steroidogenesis and epididymal function by inhaled benzo[a]pyrene. Reprod Toxicol 17: 527-537. doi: 10.1016/S0890-6238(03)00071-6
    [4] Perera FP, Santella RM, Brenner D, et al. (1989) Application of biological markers to the study of lung cancer causation and prevention. IARC Sci 89: 451-459.
    [5] Chao MR, Wang CJ, Wu MT, et al. (2008) Repeated measurements of urinary methylated/oxidative DNA lesions, acute toxicity, and mutagenicity in coke-oven workers. Cancer Epidemiol Biomarkers Prev 17: 3381-3389. doi: 10.1158/1055-9965.EPI-08-0721
    [6] Liu AL, Lu WQ, Wang ZZ (2006) Elevated levels of urinary 8-hydroxy-2-deoxyguanosine, lymphocytic micronuclei, and serum glutathione S-transferase in workers exposed to coke oven emissions. Environ Health Perspect 114: 673-677.
    [7] Park JH, Troxel AB, Harvey RG, et al. (2006) Polycyclic aromatic hydrocarbon (PAH) o-quinones produced by the aldo-keto-reductases (AKRs) generate abasic sites, oxidized pyrimidines, and 8-oxo-dGuo via reactive oxygen species. Chem Res Toxicol 19: 719-728. doi: 10.1021/tx0600245
    [8] Cavalieri EL, Rogan EG (1995) Central role of radical cations in metabolic activation of polycyclic aromatic hydrocarbons. Xenobiotica 25: 677-678. doi: 10.3109/00498259509061885
    [9] Cooper CS, Grover PL, Sim P (1983) The metabolism and activation of benzo[a]pyrene. Prog Drug Metab 7: 297-396.
    [10] Grollman AP, Moriya M (1993) Mutagenesis by 8-oxoguanine: an enemy within. Trends Genet 9: 246-249. doi: 10.1016/0168-9525(93)90089-Z
    [11] Nuernberg AM, Boyce PD, Cavallari JM, et al. (2008) Urinary 8-isoprostane and 8-OHdG concentrations in boilermakers with welding exposure. J Occup Environ Med 50: 182-189. doi: 10.1097/JOM.0b013e31815cf6cc
    [12] Singh R, Sram RJ, Binkova B, et al. (2007) The relationship between biomarkers of oxidative DNA damage, polycyclic aromatic hydrocarbon DNA adducts, antioxidant status and genetic susceptibility following exposure to environmental air pollution in humans. Mutat Res 620: 83-92. doi: 10.1016/j.mrfmmm.2007.02.025
    [13] Cheng KC, Cahhill DS, Kasai H, et al. (1992) 8-hydroxyguanine, an abundant form of oxidative DNA damage, causes G-T and A-C substitutions. J Biol Chem 267: 166-172.
    [14] Singh R, Sram RJ, Binkova B, et al. (2007) The relationship between biomarkers of oxidative DNA damage, polycyclic aromatic hydrocarbon DNA adducts antioxidant status and genetic susceptibility following exposure to environmental air pollution in humans. Mutat Res 620: 83-92. doi: 10.1016/j.mrfmmm.2007.02.025
    [15] Guz J, Gachowski F M, Rozalski R, et al. (2013) Comparison of Oxidative Stress/DNA Damage in Semen and Blood of Fertile and Infertile Men. PLoS One 8: e68490. doi: 10.1371/journal.pone.0068490
    [16] Evans MD, Olinski R, Loft S, et al. (2010) Toward consensus in the analysis of urinary 8-oxo-7, 8-dihydro-29-deoxyguanosine as a noninvasive biomarker of oxidative stress. FASEB J 24: 1249-1260. doi: 10.1096/fj.09-147124
    [17] Zenzes MT, Bielechi R, Reed TE (1999). Detection of benzo(a)pyrene diol epoxides-DNA adducts in sperm of men exposed to cigarette smoke. Fertil Steril 72: 330-335. doi: 10.1016/S0015-0282(99)00230-7
    [18] Binková B, Lewtas J, Misková I, et al. (1995) DNA adducts and personal air monitoring of carcinogenic polycyclic aromatic hydrocarbons in an environmentally exposed population. Carcinogenesis 16: 1037-1046. doi: 10.1093/carcin/16.5.1037
    [19] Revel A, Raanani H, Younglai E, et al. (2001) Resveratrol, a natural aryl hydrocarbon receptor antagonist, protects sperm from DNA damage and apoptosis caused by benzo(a)pyrene. Reprod Toxicol 15: 479-486. doi: 10.1016/S0890-6238(01)00149-6
    [20] Park JH, Troxel AB, Harvey RG, et al. (2006) Polycyclic aromatic hydrocarbon (PAH) o-quinones produced by the aldo-keto-reductases (AKRs) generate abasic sites, oxidized pyrimidines, and 8-oxo-dGuo via reactive oxygen species. Chem Res Toxicol 19: 719-728. doi: 10.1021/tx0600245
    [21] Cheng KC, Cahhill DS, Kasai H, et al. (1993) 8-hydroxyguanine, an abundant form of oxidative DNA damage, causes G-T and A-C substitutions. J Biol Chem 267: 166-172.
    [22] Hu CW, Chao MR, Sie CH (2010) Urinary analysis of 8-oxo-7,8-dihydroguanine and 8-oxo-7,8-dihydro-2'-deoxyguanosine by isotope-dilution LC-MS/MS with automated solid-phase extraction: Study of 8-oxo-7,8-dihydroguanine stability. Free Radic Biol Med 48: 89-97. doi: 10.1016/j.freeradbiomed.2009.10.029
    [23] Sawyer DE, Mercer BG, Wiklendt AM, et al. (2003) Quantitative analysis of gene-specific DNA damage in human spermatozoa. Mutat Res 529: 21-34. doi: 10.1016/S0027-5107(03)00101-5
    [24] Paracchini V, Chang SS, et al. (2005) GSTM1 [corrected] deletion modifies the levels of polycyclic aromatic hydrocarbon-DNA adducts in human sperm. Mutat Res 586: 97-101. doi: 10.1016/j.mrgentox.2005.06.008
    [25] Gaspari L, Chang SS, Santella RM, et al. (2003) Polycyclic aromatic hydrocarbon-DNA adducts in human sperm as a marker of DNA damage and infertility. Mutat Res 535: 155-160. doi: 10.1016/S1383-5718(02)00297-8
    [26] Spano M, Bonde JP, Hjollund HI (2000) Sperm chromatin damage impairs human fertility. Fertil Steril 73: 43-50. doi: 10.1016/S0015-0282(99)00462-8
    [27] Klungland A, Bjelland S (2007) Oxidative damage to purines in DNA: role of mammalian Ogg1. DNA Repair (Amst) 6: 481-488. doi: 10.1016/j.dnarep.2006.10.012
    [28] Gedik CM, Collins A (2005) ESCODD (European Standards Committee on Oxidative DNA Damage) Establishing the background level of base oxidation in humanlymphocyte DNA: results of an interlaboratory validation study. FASEB J 19: 82-84.
    [29] Guetens G, De Boech, G, Highley M, et al. (2002) Oxidative DNA damage: biological significance and methods of analysis. Crit Rev Clin Lab Sci 39:3310-457.
    [30] Holmberg I, Stal P, Hamberg M (1999) Quantitative determination of 8-hydroxy-2'-deoxyguanosine in human urine by isotope dilution mass spectrometry normal levels in hemochromatosis. Free Radical Bio Med 26: 126-129.
    [31] Dizdaroglu M, Jaruga P, Birincioglu M, et al. (2002) Free radical-induced damage to DNA mechanisms and measurement. Free Radical Biol Med 32: 1102-1115. doi: 10.1016/S0891-5849(02)00826-2
    [32] Cooke MS, Singh R, Hall GK, et al. (2006) Evaluation of enzyme-linked immunosorbent assay and liquid chromatography-tandem mass spectrometry methodology for the analysis of 8-oxo-7, 8-dihydro-2'-deoxyguanosine in saliva and urine. Free Radic Biol Med 41: 1829-1836. doi: 10.1016/j.freeradbiomed.2006.09.009
    [33] Ravanat JL, Duretz B, Guiller A, et al. (1998) Isotope dilution high-performance liquid chromatography-electrospray tandem mass spectrometry assay for the measurement of 8-oxo-7,8-dihydro-2'-deoxy-guanosine in biological samples. J Chromatogr B 715: 349-356. doi: 10.1016/S0378-4347(98)00259-X
    [34] World Health Organization. (2010) WHO laboratory manual for the examination and processing of human semen. WHO, 5.
    [35] Evenson DP, Wixon R (2005) Environmental toxicants cause sperm DNA fragmentation as detected by the sperm chromatin structure assay. Toxicol Appl Pharmacol 207: S532-537. doi: 10.1016/j.taap.2005.03.021
    [36] Evenson DP, Larson KL, Jost LK (2002) Sperm chromatin structure assay: its clinical use for detecting sperm DNA fragmentation in male infertility and comparisons with other techniques. J Androl 23: 25-43.
    [37] Gedik CM, Collins A. ESCODD (European Standards Committee on Oxidative DNA Damage) (2005) Establishing the background level of base oxidation in human lymphocyte DNA: results of an interlaboratory validation study. FASEB J 19: 82-84.
    [38] Chao MR, Yen CC, Hu CW (2008) Prevention of artifact oxidation in determination of cellular 8-oxo-7,8-dihydro-2'-deoxyguanosine by isotope-dilution LC-MS/MS with automated solid-phase extraction. Free Radic Biol Med 44: 464-473. doi: 10.1016/j.freeradbiomed.2007.10.003
    [39] Rubes J, Selevan SG, Evenson D P, et al. (2005). Episodic air pollution is associated with increased DNA fragmentation in human sperm without other changes in semen quality. Hum Reprod 20: 2776-2783. doi: 10.1093/humrep/dei122
    [40] Zribi N, Chakroun NF, Elleuch H, et al. (2011) Sperm DNA fragmentation and oxidation are independent of malondialdheyde. Reprod Biol Endocrinol 9: 47. doi: 10.1186/1477-7827-9-47
    [41] Singh S, Sharma S, Jain M, et al. (2011) Importance of papanicolaou staining for sperm morphologic analysis: comparison with an automated sperm quality analyzer. Am J Clin Pathol 136: 247-251. doi: 10.1309/AJCPCLCSPP24QPHR
    [42] Sheridan J, Wang LM, Tosetto M, et al. (2009) Nuclear oxidative damage correlates with poor survival in colorectal cancer. British J Cancer 100: 381-388. doi: 10.1038/sj.bjc.6604821
    [43] Twigg J, Irvine DS, Houston P, et al. (1998) Iatrogenic DNA damage induced in human psermatozoa during sperm preparation: protective significance of seminal plasma. Mol Hum Reprod 4: 439-445. doi: 10.1093/molehr/4.5.439
    [44] Kao SH, Chao HT, Chen HW, et al. (2008) Increase of oxidative stress in human sperm with lower motility. Fertil Steril 89: 1183-1190. doi: 10.1016/j.fertnstert.2007.05.029
    [45] Aitken RJ, Baker MA, De Iuliis GN, et al. (2010) New insights into sperm physiology and pathology. Handb Exp Pharmacol 198: 99-115. doi: 10.1007/978-3-642-02062-9_7
    [46] De Vries M, Ramos L, Housein Z, et al. (2012) Chromatin remodelling initiation during human spermiogenesis. Biol Open 1: 446-457. doi: 10.1242/bio.2012844
    [47] Köberle B, Masters JRW, Hartley JA, et al. (1999) Defective repair of cisplatin-induced DNA damage caused by reduced XPA protein in testicular germ cell tumors. Current Bio 9: 273-278. doi: 10.1016/S0960-9822(99)80118-3
    [48] Smith R, Vantman D, Ponce J, et al. (1996) Total antioxidant capacity of human seminal plasma. Hum Reprod 11:1655-60. doi: 10.1093/oxfordjournals.humrep.a019465
    [49] Thiele JJ, Friesleben HJ, Fuchs J, et al. (1995) Ascorbic acid and urate in human seminal plasma: determination and interrelationships with chemiluminescence in washed semen. Hum Reprod 10: 110-115. doi: 10.1093/humrep/10.1.110
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