Crude oil degradation potential of bacteria isolated from oil-polluted soil and animal wastes in soil amended with animal wastes

Voke O. Urhibo; Bernard O. Ejechi; Voke O. Urhibo; Bernard O. Ejechi

doi:10.3934/environsci.2017.2.277

AIMS Environmental Science

2017, Volume 4, Issue 2: 277-286. doi: 10.3934/environsci.2017.2.277

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Crude oil degradation potential of bacteria isolated from oil-polluted soil and animal wastes in soil amended with animal wastes

Voke O. Urhibo ¹,
Bernard O. Ejechi ^{2
,
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1.
Department of Environmental Management and Toxicology, Federal University of Petroleum Resources, Effurun, Nigeria
2.
Department of Microbiology, Delta State University, Abraka, Nigeria

Received: 04 December 2016 Accepted: 07 March 2017 Published: 15 March 2017

The influence of animal wastes on crude oil degradation potential of strains of Proteus vulgaris and Bacillus subtilis isolated from animal wastes (poultry and pig droppings) and petroleum-polluted soil was compared in laboratory studies. Both bacterial strains were selected for high crude oil degradation ability after screening many isolates by the 2,6-dichlorophenol indophenol method. Analyses by gas chromatography (GC) showed that degradation of crude oil was markedly enhanced (88.3–97.3% vs 72.1–78.8%) in soil amended with animal wastes as indicated by the reduction of total petroleum hydrocarbon (TPH). TPH reduction by animal waste bacterial strains in animal waste-amended soil was more than the reduction by strains from soil contaminated with petroleum (P < 0.001). The greatest reduction of TPH (96.6–97.3% vs 80.4–95.9%) was by poultry waste strains and it occurred in soil amended with poultry waste. GC analyses of n-alkanes showed that although shorter chains were preferentially degraded [32.0–78.5% (C8–23) vs 6.3–18.5% (C24–36)] in normal soil, biodegradation of longer chains increased to 38.4–46.3% in animal waste-amended soil inoculated with the same animal wastes’ strains. The results indicate that these animal waste strains may be of potential application for bioremediation of oil-polluted soil in the presence of the wastes from where they were isolated.
- poultry wastes,
- pig wastes,
- crude oil,
- biodegradation,
- Proteus vulgaris,
- Bacillus subtilis
Citation: Voke O. Urhibo, Bernard O. Ejechi. Crude oil degradation potential of bacteria isolated from oil-polluted soil and animal wastes in soil amended with animal wastes[J]. AIMS Environmental Science, 2017, 4(2): 277-286. doi: 10.3934/environsci.2017.2.277

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Abstract

The influence of animal wastes on crude oil degradation potential of strains of Proteus vulgaris and Bacillus subtilis isolated from animal wastes (poultry and pig droppings) and petroleum-polluted soil was compared in laboratory studies. Both bacterial strains were selected for high crude oil degradation ability after screening many isolates by the 2,6-dichlorophenol indophenol method. Analyses by gas chromatography (GC) showed that degradation of crude oil was markedly enhanced (88.3–97.3% vs 72.1–78.8%) in soil amended with animal wastes as indicated by the reduction of total petroleum hydrocarbon (TPH). TPH reduction by animal waste bacterial strains in animal waste-amended soil was more than the reduction by strains from soil contaminated with petroleum (P < 0.001). The greatest reduction of TPH (96.6–97.3% vs 80.4–95.9%) was by poultry waste strains and it occurred in soil amended with poultry waste. GC analyses of n-alkanes showed that although shorter chains were preferentially degraded [32.0–78.5% (C8–23) vs 6.3–18.5% (C24–36)] in normal soil, biodegradation of longer chains increased to 38.4–46.3% in animal waste-amended soil inoculated with the same animal wastes’ strains. The results indicate that these animal waste strains may be of potential application for bioremediation of oil-polluted soil in the presence of the wastes from where they were isolated.

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