Biotransformation of As (III) to As (V) and their stabilization in soil with <em>Bacillus</em> sp. XS2 isolated from gold mine tailing of Xinjiang, China

Santosh Kumar Karn; Xiangliang Pan; Santosh Kumar Karn; Xiangliang Pan

doi:10.3934/environsci.2016.4.592

AIMS Environmental Science

2016, Volume 3, Issue 4: 592-603. doi: 10.3934/environsci.2016.4.592

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Research article

Biotransformation of As (III) to As (V) and their stabilization in soil with Bacillus sp. XS2 isolated from gold mine tailing of Xinjiang, China

Santosh Kumar Karn ^{1,2
,},
Xiangliang Pan ^{1
,
,}

1.
Environmental Pollution and Bioremediation Laboratory, Xinjiang Institute of Ecology and Geography, Chinese Academy of Science, Urumqi-830011 Xinjiang, PR of China
2.
Department of Biotechnology, National Institute of Technology, Raipur 492010 (CG), India

Received: 16 May 2016 Accepted: 26 September 2016 Published: 27 September 2016

Xinjiang province is one of the most polluted region in China, this region affected by multi-metal problem, especially arsenic (As) affect very badly. Two major forms of As present in the environment As (III) and As (V) as compared to As (V), As (III) is much more toxic. Our aim is to remediate As (III) contaminated soil by using As (III) resistant microbe, having the high transforming ability for As (III) to As (V). An As (III) oxidizing bacterium Bacillus sp. XS2, isolated and selected from gold mine tailing which have shown high resistance up to 6400 mg L⁻¹ and efficiently transformed up to 4000 mg L⁻¹ in sucrose low phosphate medium (SLP), higher than any of the previous reported Bacillus sp.. In soil, we found that XS2 successfully transformed up to 81% of soluble exchangeable fraction within 10 d in contaminated soil (with 500 mg kg⁻¹), which makes it potential microbe for the removal of contaminated site with arsenic in the environment. Further XS2 was characterized for their molecular basis of resistance and we establish that XS2 having arsenic oxidase enzyme activity which is accountable for the detoxification of arsenic at high concentration and provide resistance to the bacterium. Gene encoding arsenic oxidase (aioA-gene) was also amplified from this bacterium using polymerase chain reaction (PCR) with degenerate primer. The aioA-gene is specific for the arsenite-oxidizing bacteria. The deduced amino acid sequence had shown 42% similarity with pseudomonas sp. arsenic oxidase. Further, the strain was characterized by 16s rRNA gene sequence analysis and shown maximum similarity with Bacillus sp.
- Gold mine tailings,
- Arsenite oxidase,
- aoxB gene,
- Arsenite oxidation,
- Bacillus sp. in-situ remediation
Citation: Santosh Kumar Karn, Xiangliang Pan. Biotransformation of As (III) to As (V) and their stabilization in soil with Bacillus sp. XS2 isolated from gold mine tailing of Xinjiang, China[J]. AIMS Environmental Science, 2016, 3(4): 592-603. doi: 10.3934/environsci.2016.4.592

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Abstract

Xinjiang province is one of the most polluted region in China, this region affected by multi-metal problem, especially arsenic (As) affect very badly. Two major forms of As present in the environment As (III) and As (V) as compared to As (V), As (III) is much more toxic. Our aim is to remediate As (III) contaminated soil by using As (III) resistant microbe, having the high transforming ability for As (III) to As (V). An As (III) oxidizing bacterium Bacillus sp. XS2, isolated and selected from gold mine tailing which have shown high resistance up to 6400 mg L⁻¹ and efficiently transformed up to 4000 mg L⁻¹ in sucrose low phosphate medium (SLP), higher than any of the previous reported Bacillus sp.. In soil, we found that XS2 successfully transformed up to 81% of soluble exchangeable fraction within 10 d in contaminated soil (with 500 mg kg⁻¹), which makes it potential microbe for the removal of contaminated site with arsenic in the environment. Further XS2 was characterized for their molecular basis of resistance and we establish that XS2 having arsenic oxidase enzyme activity which is accountable for the detoxification of arsenic at high concentration and provide resistance to the bacterium. Gene encoding arsenic oxidase (aioA-gene) was also amplified from this bacterium using polymerase chain reaction (PCR) with degenerate primer. The aioA-gene is specific for the arsenite-oxidizing bacteria. The deduced amino acid sequence had shown 42% similarity with pseudomonas sp. arsenic oxidase. Further, the strain was characterized by 16s rRNA gene sequence analysis and shown maximum similarity with Bacillus sp.

References

[1]	Rhine ED, Phelps CD, Young LY (2006) Anaerobic arsenite oxidation by novel denitrifying isolates. Environ Microbiol 8: 899-908. doi: 10.1111/j.1462-2920.2005.00977.x
[2]	Sun G (2004) Arsenic contamination and arsenicosis in China. Toxicol Applied Pharmacol 198: 268-271.
[3]	Wen D, Zhang F, Zhang E, et al. (2013) Arsenic, fluoride and iodine in groundwater of China. J Geochem Explor 135: 1-21. doi: 10.1016/j.gexplo.2013.10.012
[4]	WHO (1993) Guidelines for drinking water quality, Vol 1: Recommendations, 2^nd ed. WHO, Geneva. Available from: http://www.who.int/water_sanitation_health/dwq/2edvol1i.pdf.
[5]	Valls M, De Lorenzo V (2002) Exploiting the genetic and biochemical capacities of bacteria for the remediation of heavy metal pollution. FEMS Microbiolo Rev 26: 327-338.
[6]	Garcia-Dominguez E, Mumford A, Rhine ED, et al. (2009) Novel autotrophic arsenite-oxidizing bacteria isolated from soil and sediments. FEMS Microbio Ecol 66: 401-410.
[7]	Muller D, Lievremont D, Simeonova DD, et al. (2003) Arsenite oxidase aox genes from a metal-resistant β-Proteobacterium. J Bacteriol 185: 135-141. doi: 10.1128/JB.185.1.135-141.2003
[8]	Chang JS, Kim YH, Kim KW (2008) The ars genotype characterization of arsenic-resistant bacteria from arsenic-contaminated gold-silver mines in the Republic of Korea. App Microbiol Biotechnol 80: 155-165.
[9]	Simeonova DD, Lievremont D, Lagarde F, et al. (2004) Microplate screening assay for the detection of arsenite-oxidizing and arsenate-reducing bacteria. FEMS Microbiol Lett 237: 249-253. doi: 10.1111/j.1574-6968.2004.tb09703.x
[10]	Glaubig RA, Goldberg S (1988) Determination of inorganic arsenic (III) and arsenic (III plus V) using automated hydride-generation atomic absorption spectrometry. Soil Sci SOC Am J 52: 536-537. doi: 10.2136/sssaj1988.03615995005200020044x
[11]	Voth-Beach LM, Shrader DE (1985) Reduction of interferences in the determination of arsenic and selenium by hydride generation. Spectroscopy 1: 60-65.
[12]	Anderson GL, Williams R, Hille J (1992) The purification and characterization of arsenite oxidase from Alcaligenes faecalis, a molybdenum-containing hydroxylase. J Biolog Chem 267: 23674-23682.
[13]	Kapley A, Lampel K, Purohit HJ (2001) Rapid detection of Salmonella in water samples by multiplex PCR. W Environ Res 73: 461-465. doi: 10.2175/106143001X139515
[14]	Inskeep WP, Macur RE, Hamamura N, et al. (2007) Detection, diversity and expression of aerobic bacterial arsenite oxidase genes. Environ Microbiol 9: 934-943.
[15]	Weisberg WA, Barns SM, Pelletier DA, et al. (1991) 16S rDNA amplification for phylogenetic study. J Bacteriol 173: 697-703.
[16]	Tamura K, Dudley J, Nei M, et al. (2007) MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol Biol Evol 24: 1596-1599. doi: 10.1093/molbev/msm092
[17]	Tessier A, Campbell PGC, Bisson M (1979) Sequential Extraction Procedure for the Speciation of Particulate Trace Metals. Analy Chem 51: 844-851. doi: 10.1021/ac50043a017
[18]	Battaglia-Brunet F, Dictor MC, Garrido F, et al. (2002) An arsenic (III)-oxidizing bacterial population: selection, characterization and performance in reactors. J App Microbiol 93: 656-667. doi: 10.1046/j.1365-2672.2002.01726.x
[19]	Weeger W, Lievremont D, Perret M, et al. (1999) Oxidation of arsenite to arsenate by a bacterium isolated from an aquatic environment. BioMetals 12: 141-149.
[20]	Chang JS, Kim IS (2010) Arsenite Oxidation by Bacillus sp. Strain SeaH-As22w Isolated from Coastal Seawater in Yeosu Bay. Environ Engg Res 15: 15-21.
[21]	Osborne FH, Enrlich HL (1976) Oxidation of arsenite by a soil isolate of Alcaligenes. J App Bacteriol 41: 295-305. doi: 10.1111/j.1365-2672.1976.tb00633.x
[22]	Salmassi TM, Venkateswaren K, Satomi M, et al. (2002) Oxidation of arsenite by Agrobacterium albertimagni, AOL15, sp. nov., isolated from Hot Creek, California. Geomicrobiol J 19: 53-66.
[23]	Gihring TM, Druschel GK, McCleskey RB, et al. (2010) Rapid arsenite oxidation by Thermusaquaticus and Thermusthermophilus: field and laboratory investigations. Environ Sci Technol 35: 3857-3862.
[24]	Chitpirom K, Tanasupawat S, Akaracharanya A, et al. (2012) Comamonas terrae sp. nov., an arsenite-oxidizing bacterium isolated from agricultural soil in Thailand. J Gen App Microbio 58: 245-251.
[25]	Pepi M, Volterrani M, Renzi1 M, et al. (2007) Arsenic-resistant bacteria isolated from contaminated sediments of the Orbetello Lagoon, Italy, and their characterization. J App Microbiol 103: 2299-2308. doi: 10.1111/j.1365-2672.2007.03471.x
[26]	Santini JM, Hoven N, Vanden R (2004) Molybdenum-containing arsenite oxidase of the chemolithoautotrophic arsenite oxidizer NT- 26. J Bacteriol 86: 1614-1619.
[27]	Prasad KS, Subramanian V, Paul JS (2009) Purification and characterization of arsenite oxidase from Arthrobacter sp.. Biometals 22: 711-721. doi: 10.1007/s10534-009-9215-6
[28]	Ellis PJ, Conrads T, Hille R, et al. (2001) Crystal structure of the 100 kDa arsenite oxidase from Alcaligenes faecalis in two crystal forms at 1.64 angstrom and 2.03 angstrom. Structure 9: 125-132.
[29]	Srivastava S, Verma PC, Singh A, et al. (2012) Isolation and characterization of Staphylococcus sp. strain NBRIEAG-8 from arsenic contaminated site of West Bengal. App Microbiol Biotechnol 95: 1275-1291.
[30]	Kirkham MB (1977) Trace elements sludge on land: Effect on plants, soils, and ground water. pp. 209-247. In: R.C. Laehr (ed.) Land as a waste management alternative. Ann Arbor Science Publishers, Ann Arbor, MI.
[31]	Manning BA, Fendorf SE, Goldberg S (1998) Surface structures and stability of arsenic (III) on goethite: Spectroscopic evidence for inner-sphere complexes. Environ Sc Technol 32: 2383-2388. doi: 10.1021/es9802201
[32]	Clevenger TE, Mullins W (1982) The toxic extraction procedure for hazardous waste. In Trace substances in environmental health XVI. Univ. of Missouri, Columbia, MO, 77-82.

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