Decontamination of digital image sensors and assessment of electron microscope performance in a BSL-3 containment

Michael B. Sherman; Juan Trujillo; Benjamin E. Bammes; Liang Jin; Matthias W. Stumpf; Scott C. Weaver; Michael B. Sherman; Juan Trujillo; Benjamin E. Bammes; Liang Jin; Matthias W. Stumpf; Scott C. Weaver

doi:10.3934/biophy.2015.2.153

AIMS Biophysics

2015, Volume 2, Issue 2: 153-162. doi: 10.3934/biophy.2015.2.153

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Decontamination of digital image sensors and assessment of electron microscope performance in a BSL-3 containment

1.
Sealy Center for Structural Biology and Molecular Biophysics, University of Texas Medical Branch, Galveston, Texas, USA;
2.
Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, Texas, USA;
3.
Institute for Human Infections and Immunity, University of Texas Medical Branch, Galveston, Texas, USA;
4.
Center for Biodefense and Emerging Infectious Diseases, University of Texas Medical Branch, Galveston, Texas, USA;
5.
JEOL USA, Inc., Peabody, Massachusetts, USA;
6.
Direct Electron, LP, San Diego, California, USA;
7.
E. A. Fischione Instruments, Inc., Export, Pennsylvania, USA;
8.
Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, Texas, USA

Received: 02 March 2015 Accepted: 17 May 2015 Published: 21 May 2015

A unique biological safety level (BSL)-3 cryo-electron microscopy facility with a 200 keV high-end cryo-electron microscope has been commissioned at the University of Texas Medical Branch (UTMB) to study the structure of viruses and bacteria classified as select agents. We developed a microscope decontamination protocol based on chlorine dioxide gas with a continuous flow system. In this paper we report on testing digital camera sensors (both CCD and CMOS direct detector) in a BSL-3 environment, and microscope performance after chlorine dioxide (ClO₂) decontamination cycles.
- cryo-electron microscopy,
- single particle imaging,
- biological safety containment
Citation: Michael B. Sherman, Juan Trujillo, Benjamin E. Bammes, Liang Jin, Matthias W. Stumpf, Scott C. Weaver. Decontamination of digital image sensors and assessment of electron microscope performance in a BSL-3 containment[J]. AIMS Biophysics, 2015, 2(2): 153-162. doi: 10.3934/biophy.2015.2.153

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Abstract

A unique biological safety level (BSL)-3 cryo-electron microscopy facility with a 200 keV high-end cryo-electron microscope has been commissioned at the University of Texas Medical Branch (UTMB) to study the structure of viruses and bacteria classified as select agents. We developed a microscope decontamination protocol based on chlorine dioxide gas with a continuous flow system. In this paper we report on testing digital camera sensors (both CCD and CMOS direct detector) in a BSL-3 environment, and microscope performance after chlorine dioxide (ClO₂) decontamination cycles.

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