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Determining complex aggregate distributions of macromolecules using photobleaching image correlation microscopy

  • Received: 12 February 2015 Accepted: 10 March 2015 Published: 16 March 2015
  • Aggregation of cell surface receptors is believed to be linked to their biological function. In this paper, we disclose the explicit formulation for macromolecular aggregation distribution by means of photobleaching Image Correlation Spectroscopy (pbICS).

    Citation: Lajevardipour Alireza, W. M. Chon James, H. A. Clayton Andrew. Determining complex aggregate distributions of macromolecules using photobleaching image correlation microscopy[J]. AIMS Biophysics, 2015, 2(1): 1-7. doi: 10.3934/biophy.2015.1.1

    Related Papers:

  • Aggregation of cell surface receptors is believed to be linked to their biological function. In this paper, we disclose the explicit formulation for macromolecular aggregation distribution by means of photobleaching Image Correlation Spectroscopy (pbICS).


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    [1] Douglas Magde, Elliot Elson, W. Webb (1972) Thermodynamic Fluctuations in a Reacting System——Measurement by Fluorescence Correlation Spectroscopy.Phys Rev Let 19: 705-708.
    [2] N. O. Petersen, P. L. H?ddelius, P. W. Wiseman, et al. (1993) Quantitation of membrane receptor distributions by image correlation spectroscopy: concept and application.Biophys J 65: 1135-1146.
    [3] Yan Chen, Joachim D. Müller, Peter T.C. So, et al. (1999) The Photon Counting Histogram in Fluorescence Fluc- tuation Spectroscopy.Biophys J 77: 553-567.
    [4] Antoine G. Godin, Santiago Costantino, Louis-Etienne Lorenzo, et al. (2011) Revealing protein oligomerization and densities in situ using spatial intensity distribution analysis.Proc Natl Acad Sci U S A 108: 7010-7015.
    [5] Michelle A. Digman, Rooshin Dalal, Alan F. Horwitz, et al. (2008) Mapping the number of molecules and brightness in the laser scanning microscope.Biophy J 94: 2320-2332.
    [6] Tedman Torres, Marcia Levitus. (2007) Measuring conformational dynamics: a new FCS-FRET approach.J Phys Chem B 111: 7392-7400.
    [7] Joseph R. Lakowicz (2006) Principles of Fluorescence Spectroscopy.Springer US .
    [8] Claire Robertson and Steven C. George (2012) Theory and practical recommendations for autocorrelation-based image correlation spectroscopy.J Biomedical Optics 17: 80801-1.
    [9] Norman R. Davidson (1962) Statistical mechanics.McGraw-Hill .
    [10] Elliot L. Elson and Douglas Magde (1974) Fluorescence correlation spectroscopy. i. conceptual basis and theory.Biopolymers 13: 1-27.
    [11] N. O. Petersen (1986) Scanning uorescence correlation spectroscopy. i. theory and simulation of aggregation mea- surements.Biophys J 49: 809-815.
    [12] Giuseppe D. Ciccotosto, Noga Kozer, Timothy T. Y. Chow, et al. (2013) Aggregation distributions on cells determined by photobleaching image correlation spectroscopy.Biophys J 104: 1056-1064.
    [13] Henry Margenau, George Moseley MurphyThe mathematics of physics and chemistry.Young Press, 2nd edition1956 .
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  • © 2015 the Author(s), licensee AIMS Press. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0)
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