Towards understanding of plant mitochondrial VDAC proteins: an overview of bean (<em>Phaseolus</em>) VDAC proteins

Hayet Saidani; Daria Grobys; Marc Léonetti; Hanna Kmita; Fabrice Homblé; Hayet Saidani; Daria Grobys; Marc Léonetti; Hanna Kmita; Fabrice Homblé

doi:10.3934/biophy.2017.1.43

AIMS Biophysics

2017, Volume 4, Issue 1: 43-62. doi: 10.3934/biophy.2017.1.43

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Towards understanding of plant mitochondrial VDAC proteins: an overview of bean (Phaseolus) VDAC proteins

1.
Structure et Fonction des Membranes Biologiques, Université Libre de Bruxelles (ULB), Boulevard du Triomphe CP 206/2, B-1050 Brussels, Belgium
2.
Laboratory of Bioenergetics, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University, Umultowska 89, 61-614 Poznan, Poland
3.
I.R.P.H.E., Aix-Marseille Université, CNRS, Technopôle de Château-Gombert, F-13384, Marseille Cedex 13, France

Received: 28 August 2016 Accepted: 13 December 2016 Published: 27 December 2016

As the main grain legume consumed worldwide, the common bean (Phaseolus vulgaris) is generally considered as a model for food legumes. The mitochondrial voltage-dependent anion-selective channel (VDAC) is the major transport pathway for inorganic ions, metabolites, and tRNA, and consequently it controls the exchange of these compounds between the cytoplasm and the mitochondrion. Two VDAC isoforms of Phaseolus coccineus have been investigated experimentally. However, plant VDACs are known to belong to a small multigenic family of variable size. Here, we combine available experimental as well as genomic and transcriptomic data to identify and characterize the VDAC family of Phaseolus vulgaris. To this aim, we review the current state of our knowledge of Phaseolus VDAC functional and structural properties. The genomic and transcriptomic data available for the putative VDACs of Phaseolus vulgaris are studied using bioinformatics approach including homology modelling. The obtained results indicate that five out of the seven putative VDAC encoding sequences (named PvVDAC1–5) share strongly conserved motifs and structural homology with known VDACs. Notably, PvVDAC4 and PvVDAC5 are very close to the two abundant and characterized experimentally VDAC isoforms purified from Phaseolus coccineus mitochondria.
- Phaseolus,
- mitochondria,
- VDAC,
- functional and structural properties
Citation: Hayet Saidani, Daria Grobys, Marc Léonetti, Hanna Kmita, Fabrice Homblé. Towards understanding of plant mitochondrial VDAC proteins: an overview of bean (Phaseolus) VDAC proteins[J]. AIMS Biophysics, 2017, 4(1): 43-62. doi: 10.3934/biophy.2017.1.43

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Abstract

As the main grain legume consumed worldwide, the common bean (Phaseolus vulgaris) is generally considered as a model for food legumes. The mitochondrial voltage-dependent anion-selective channel (VDAC) is the major transport pathway for inorganic ions, metabolites, and tRNA, and consequently it controls the exchange of these compounds between the cytoplasm and the mitochondrion. Two VDAC isoforms of Phaseolus coccineus have been investigated experimentally. However, plant VDACs are known to belong to a small multigenic family of variable size. Here, we combine available experimental as well as genomic and transcriptomic data to identify and characterize the VDAC family of Phaseolus vulgaris. To this aim, we review the current state of our knowledge of Phaseolus VDAC functional and structural properties. The genomic and transcriptomic data available for the putative VDACs of Phaseolus vulgaris are studied using bioinformatics approach including homology modelling. The obtained results indicate that five out of the seven putative VDAC encoding sequences (named PvVDAC1–5) share strongly conserved motifs and structural homology with known VDACs. Notably, PvVDAC4 and PvVDAC5 are very close to the two abundant and characterized experimentally VDAC isoforms purified from Phaseolus coccineus mitochondria.

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