Intrinsic role of coagulase negative staphylococci <em>norA</em>-like efflux system in fluoroquinolones resistance

Ligozzi Marco; Galia Liliana; Bertoncelli Anna; Mazzariol Annarita; Ligozzi Marco; Galia Liliana; Bertoncelli Anna; Mazzariol Annarita

doi:10.3934/microbiol.2017.4.908

AIMS Microbiology

2017, Volume 3, Issue 4: 908-914. doi: 10.3934/microbiol.2017.4.908

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Intrinsic role of coagulase negative staphylococci norA-like efflux system in fluoroquinolones resistance

Department of Diagnostics and Public Health, University of Verona, Verona, Italy

Received: 13 August 2017 Accepted: 06 November 2017 Published: 14 November 2017

NorA is a Staphylococcus aureus multidrug transporter that exports structurally distinct compounds including fluoroquinolones. In this study norA-like genes of Staphylococcus epidermidis (norA_SEP) and Staphylococcus haemolyticus (norA_SHAE) were identified and sequenced. The nucleotide identity of norA_SEP and norA_SHAE with norA was 75.3 and 74.1%, respectively, and the amino acid identity 87.7 and 86%, respectively. Inactivation of norA_SEP increased the ciprofloxacin susceptibility of E. coli DH5α carrying the pB SK 198 norA_SEP EZ cat norA_SEP plasmid.
- S. epidermidis,
- NorA-like gene,
- efflux pumps,
- S. haemolyticus,
- coagulase negative staphylococci
Citation: Ligozzi Marco, Galia Liliana, Bertoncelli Anna, Mazzariol Annarita. Intrinsic role of coagulase negative staphylococci norA-like efflux system in fluoroquinolones resistance[J]. AIMS Microbiology, 2017, 3(4): 908-914. doi: 10.3934/microbiol.2017.4.908

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Abstract

NorA is a Staphylococcus aureus multidrug transporter that exports structurally distinct compounds including fluoroquinolones. In this study norA-like genes of Staphylococcus epidermidis (norA_SEP) and Staphylococcus haemolyticus (norA_SHAE) were identified and sequenced. The nucleotide identity of norA_SEP and norA_SHAE with norA was 75.3 and 74.1%, respectively, and the amino acid identity 87.7 and 86%, respectively. Inactivation of norA_SEP increased the ciprofloxacin susceptibility of E. coli DH5α carrying the pB SK 198 norA_SEP EZ cat norA_SEP plasmid.

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