Mammalian cell adhesion on different 3D printed polymers with varying sterilization methods and acidic treatment

Sven Sölmann; Anke Rattenholl; Hannah Blattner; Guido Ehrmann; Frank Gudermann; Dirk Lütkemeyer; Andrea Ehrmann; Sven Sölmann; Anke Rattenholl; Hannah Blattner; Guido Ehrmann; Frank Gudermann; Dirk Lütkemeyer; Andrea Ehrmann

doi:10.3934/bioeng.2021004

AIMS Bioengineering

2021, Volume 8, Issue 1: 25-35. doi: 10.3934/bioeng.2021004

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Mammalian cell adhesion on different 3D printed polymers with varying sterilization methods and acidic treatment

1.
Faculty of Engineering and Mathematics, Bielefeld University of Applied Sciences, Bielefeld, Germany
2.
Virtual Institute of Applied Research on Advanced Materials (VIARAM)

Received: 22 September 2020 Accepted: 16 November 2020 Published: 26 November 2020

Cell growth on three-dimensional objects is of high interest for bioprocess engineering of adherent cells, tissue engineering and other biomedical applications. 3D printing by fusion deposition modeling (FDM) is a cheap possibility to generate tailor-made substrates for cell growth. Nevertheless, not all materials are chemically attractive for cells. Polylactic acid (PLA) and polyethylene terephthalate glycol (PETG) have been reported to be suitable polymers for tissue engineering. Thus, they might also be applicable for cost-effective bioprocessing of adherent cell lines. Here we report on the influence of printing material, surface structure, and sterilization method on Chinese hamster ovary (CHO) cell adhesion on a modified, high temperature resistant PLA, a PETG blend, and unmodified PETG, respectively. Our study revealed that CHO cells grew on all polymers tested without further surface modification. Samples could be efficiently chemically sterilized. Additional acid treatment had no significant effect on cell adhesion.
- 3D printing,
- FDM printing,
- sterilization,
- adherent cells,
- CHO cells
Citation: Sven Sölmann, Anke Rattenholl, Hannah Blattner, Guido Ehrmann, Frank Gudermann, Dirk Lütkemeyer, Andrea Ehrmann. Mammalian cell adhesion on different 3D printed polymers with varying sterilization methods and acidic treatment[J]. AIMS Bioengineering, 2021, 8(1): 25-35. doi: 10.3934/bioeng.2021004

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Abstract

Cell growth on three-dimensional objects is of high interest for bioprocess engineering of adherent cells, tissue engineering and other biomedical applications. 3D printing by fusion deposition modeling (FDM) is a cheap possibility to generate tailor-made substrates for cell growth. Nevertheless, not all materials are chemically attractive for cells. Polylactic acid (PLA) and polyethylene terephthalate glycol (PETG) have been reported to be suitable polymers for tissue engineering. Thus, they might also be applicable for cost-effective bioprocessing of adherent cell lines. Here we report on the influence of printing material, surface structure, and sterilization method on Chinese hamster ovary (CHO) cell adhesion on a modified, high temperature resistant PLA, a PETG blend, and unmodified PETG, respectively. Our study revealed that CHO cells grew on all polymers tested without further surface modification. Samples could be efficiently chemically sterilized. Additional acid treatment had no significant effect on cell adhesion.

Conflict of interest

All authors declare no conflicts of interest in this paper.

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