Coupling (reduced) Graphene Oxide to Mammalian Primary Cortical Neurons<em> In Vitro</em>

Antonina M. Monaco; Anastasiya Moskalyuk; Jaroslaw Motylewski; Farnoosh Vahidpour; Andrew M. H. Ng; Kian Ping Loh; Milos Nesládek; Michele Giugliano; Antonina M. Monaco; Anastasiya Moskalyuk; Jaroslaw Motylewski; Farnoosh Vahidpour; Andrew M. H. Ng; Kian Ping Loh; Milos Nesládek; Michele Giugliano

doi:10.3934/matersci.2015.3.217

AIMS Materials Science

2015, Volume 2, Issue 3: 217-229. doi: 10.3934/matersci.2015.3.217

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Coupling (reduced) Graphene Oxide to Mammalian Primary Cortical Neurons In Vitro

1.
Theoretical Neurobiology and Neuroengineering Laboratory, Dept. of Biomedical Sciences, University of Antwerp, Universiteitsplein 1, 2610 Wilrijk, Belgium
2.
Institute for Materials Research, Material Physics Division, Hasselt University, 3590 Diepenbeek, Belgium; IMOMEC associated laboratory, IMEC, Kapeldreef 75, 3001 Leuven, Belgium
3.
Institute of Materials Research and Engineering, Agency for Science, Technology and Research, 3 Research Link, Singapore 117602
4.
Department of Chemistry and Graphene Research Centre, National University of Singapore, 3 Science Drive 3, Singapore 117543
5.
Brain Mind Institute, Swiss Federal Institute of Technology Lausanne, Switzerland; and Dept. Computer Science, University of Sheffield, S1 4DP, UK

Received: 14 May 2015 Accepted: 03 August 2015 Published: 11 August 2015

Neuronal nanoscale interfacing aims at identifying or designing nanostructured smart materials and validating their applications as novel biocompatible scaffolds with active properties for neuronal networks formation, nerve regeneration, and bidirectional biosignal coupling. Among several carbon-based nanomaterials, Graphene recently attracted great interest for biological applications, given its unique mechanical, optical, electronic properties, and its recent technological applications. Here we explore the use of Graphene Oxide (GO) and reduced Graphene Oxide (rGO) as biocompatible culture substrates for primary neuronal networks developing ex vivo. We quantitatively studied cytotoxicity and cellular viability as well as single-cell and network-level electrophysiological properties of neurons in vitro. Our results confirm previous reports, employing immortalized cell lines or pluripotent stem cells, and extend them to mammalian primary cortical neurons: GO and rGO are biocompatible substrates and do not alter neuronal excitable properties.
- graphene oxide,
- neuroengineering,
- primary neuronal cultures,
- cellular electrophysiology,
- cytotoxicity
Citation: Antonina M. Monaco, Anastasiya Moskalyuk, Jaroslaw Motylewski, Farnoosh Vahidpour, Andrew M. H. Ng, Kian Ping Loh, Milos Nesládek, Michele Giugliano. Coupling (reduced) Graphene Oxide to Mammalian Primary Cortical Neurons In Vitro[J]. AIMS Materials Science, 2015, 2(3): 217-229. doi: 10.3934/matersci.2015.3.217

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Abstract

Neuronal nanoscale interfacing aims at identifying or designing nanostructured smart materials and validating their applications as novel biocompatible scaffolds with active properties for neuronal networks formation, nerve regeneration, and bidirectional biosignal coupling. Among several carbon-based nanomaterials, Graphene recently attracted great interest for biological applications, given its unique mechanical, optical, electronic properties, and its recent technological applications. Here we explore the use of Graphene Oxide (GO) and reduced Graphene Oxide (rGO) as biocompatible culture substrates for primary neuronal networks developing ex vivo. We quantitatively studied cytotoxicity and cellular viability as well as single-cell and network-level electrophysiological properties of neurons in vitro. Our results confirm previous reports, employing immortalized cell lines or pluripotent stem cells, and extend them to mammalian primary cortical neurons: GO and rGO are biocompatible substrates and do not alter neuronal excitable properties.

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