Preparation of <i>Teucrium polium</i> extract-loaded chitosan-sodium lauryl sulfate beads and chitosan-alginate films for wound dressing application

Mariem Kharroubi; Fatima Bellali; Abdelhafid Karrat; Mohamed Bouchdoug; Abderrahim Jaouad; Mariem Kharroubi; Fatima Bellali; Abdelhafid Karrat; Mohamed Bouchdoug; Abderrahim Jaouad

doi:10.3934/publichealth.2021059

AIMS Public Health

2021, Volume 8, Issue 4: 754-775. doi: 10.3934/publichealth.2021059

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Research article

Preparation of Teucrium polium extract-loaded chitosan-sodium lauryl sulfate beads and chitosan-alginate films for wound dressing application

1.
Laboratory of Biotechnologies, Specialized Center of Valorization and Technology of Sea Products, National Institute of Fisheries Research (INRH), Agadir, Morocco
2.
Laboratory of Biological Engineering, Faculty of Science and Technology, Beni Mellal University Sultan Moulay Slimane, Morocco
3.
Research Team of Innovation and Sustainable Development & Expertise in Green Chemistry, “ERIDDECV”, Department of Chemistry, Cadi Ayyad University, Marrakesh, Morocco

Received: 16 August 2021 Accepted: 25 September 2021 Published: 29 October 2021

This study aimed to formulate sodium lauryl sulfate cross-linked chitosan beads and sodium alginate-chitosan films for designing a dressing that would shorten the healing time of skin wounds. Teucrium polium extract-loaded chitosan-sodium lauryl sulfate beads (CH-SLS) and chitosan-alginate (CH-ALG) films were prepared and characterized by using Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD) analysis, and scanning electron microscopy (SEM). The swelling properties of the CH-SLS beads were also analyzed in a water solution. The obtained Teucrium polium extract-loaded CH-SLS beads and CH-ALG films (TBF) were further incorporated into the commercial adhesive dressing. This TBF wound dressing was then investigated for evaluation of its wound healing potential in the mice using the excision wound model. Healing was assessed by the macroscopic appearance and the rate of wound contraction during 8 days. On day 4, the TBF-treated wounds exhibited 98% reduction in the wound area when they were compared with healing ointment, elastic adhesive dressing, and untreated wounds which were exhibited 63%, 43%, and 32%, respectively. Furthermore, the application of TBF dressing reduced skin wound rank scores and increased the percentage of wounds contraction. These results demonstrate that TBF dressing improves considerably the healing rate and the macroscopic wound appearance at a short delay and this application may have therapeutic benefits in wound healing.
- chitosan-sodium lauryl sulfate beads,
- chitosan-alginate films,
- characterization,
- Teucrium polium,
- wound dressing,
- wound healing
Citation: Mariem Kharroubi, Fatima Bellali, Abdelhafid Karrat, Mohamed Bouchdoug, Abderrahim Jaouad. Preparation of Teucrium polium extract-loaded chitosan-sodium lauryl sulfate beads and chitosan-alginate films for wound dressing application[J]. AIMS Public Health, 2021, 8(4): 754-775. doi: 10.3934/publichealth.2021059

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Abstract

This study aimed to formulate sodium lauryl sulfate cross-linked chitosan beads and sodium alginate-chitosan films for designing a dressing that would shorten the healing time of skin wounds. Teucrium polium extract-loaded chitosan-sodium lauryl sulfate beads (CH-SLS) and chitosan-alginate (CH-ALG) films were prepared and characterized by using Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD) analysis, and scanning electron microscopy (SEM). The swelling properties of the CH-SLS beads were also analyzed in a water solution. The obtained Teucrium polium extract-loaded CH-SLS beads and CH-ALG films (TBF) were further incorporated into the commercial adhesive dressing. This TBF wound dressing was then investigated for evaluation of its wound healing potential in the mice using the excision wound model. Healing was assessed by the macroscopic appearance and the rate of wound contraction during 8 days. On day 4, the TBF-treated wounds exhibited 98% reduction in the wound area when they were compared with healing ointment, elastic adhesive dressing, and untreated wounds which were exhibited 63%, 43%, and 32%, respectively. Furthermore, the application of TBF dressing reduced skin wound rank scores and increased the percentage of wounds contraction. These results demonstrate that TBF dressing improves considerably the healing rate and the macroscopic wound appearance at a short delay and this application may have therapeutic benefits in wound healing.

Acknowledgments

The authors thank Dr Abouabdillah Rachid for the availability of laboratory mice.

Ethical statement

We declare that all animal experiments in this study were in accordance to the guidelines for animal care and use of the Directive 2010/63/EU (European Parliament and Council of the European Union, 2010) and EU Directive 86/609/EEC (Council of the European Communities, 1986 on the approximation of the laws, regulations and administrative provisions of the Member States concerning the protection of animals used for experimental and other scientific purposes).

Conflict of interest

The authors declare no conflict of interest.

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