Diversity and biological activity of culturable endophytic bacteria associated with marigold (<i>Calendula officinalis</i> L.)

Vyacheslav Shurigin; Burak Alaylar; Kakhramon Davranov; Stephan Wirth; Sonoko Dorothea Bellingrath-Kimura; Dilfuza Egamberdieva; Vyacheslav Shurigin; Burak Alaylar; Kakhramon Davranov; Stephan Wirth; Sonoko Dorothea Bellingrath-Kimura; Dilfuza Egamberdieva

doi:10.3934/microbiol.2021021

AIMS Microbiology

2021, Volume 7, Issue 3: 336-353. doi: 10.3934/microbiol.2021021

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Research article

Diversity and biological activity of culturable endophytic bacteria associated with marigold (Calendula officinalis L.)

Vyacheslav Shurigin ^1,3,
Burak Alaylar ²,
Kakhramon Davranov ³,
Stephan Wirth ⁴,
Sonoko Dorothea Bellingrath-Kimura ^4,5,
Dilfuza Egamberdieva ^{1,4
,
,}

1.
Department of Microbiology and Biotechnology, Faculty of Biology, National University of Uzbekistan, 100174, Tashkent, Uzbekistan
2.
Department of Molecular Biology and Genetics, Faculty of Arts and Sciences, Agri Ibrahim Cecen University, 04100, Agri, Turkey
3.
Institute of Microbiology of the Academy of Sciences of the Republic of Uzbekistan, 100128 Tashkent, Uzbekistan
4.
Leibniz Centre for Agricultural Landscape Research (ZALF), 15374 Müncheberg, Germany
5.
Faculty of Life Science, Humboldt University of Berlin, 14195, Berlin, Germany

Received: 23 July 2021 Accepted: 09 September 2021 Published: 13 September 2021

Endophytes colonizing plant tissue play an essential role in plant growth, development, stress tolerance and plant protection from soil-borne diseases. In this study, we report the diversity of cultivable endophytic bacteria associated with marigold (Calendula officinalis L.) by using 16S rRNA gene analysis and their plant beneficial properties. A total of 42 bacterial isolates were obtained from plant tissues of marigold. They belonged to the genera Pantoea, Enterobacter, Pseudomonas, Achromobacter, Xanthomonas, Rathayibacter, Agrobacterium, Pseudoxanthomonas, and Beijerinckia. Among the bacterial strains, P. kilonensis FRT12, and P. rhizosphaerae FST5 showed moderate or vigorous inhibition against three tested plant pathogenic fungi, F. culmorum, F. solani and R. solani. They also demonstrated the capability to produce hydrolytic enzymes and indole-3-acetic acid (IAA). Five out of 16 isolates significantly stimulated shoot and root growth of marigold in a pot experiment. The present study reveals that more than half of the bacterial isolates associated with marigold (C. officinalis L.) provided antifungal activity against one or more plant pathogenic fungi. Our findings suggest that medicinal plants with antimicrobial activity could be a source for selecting microbes with antagonistic activity against fungal plant pathogens or with plant growth stimulating potential. These isolates might be considered as promising candidates for the improvement of plant health.
- antagonism,
- auxin,
- endophyte,
- marigold,
- plant growth promotion
Citation: Vyacheslav Shurigin, Burak Alaylar, Kakhramon Davranov, Stephan Wirth, Sonoko Dorothea Bellingrath-Kimura, Dilfuza Egamberdieva. Diversity and biological activity of culturable endophytic bacteria associated with marigold (Calendula officinalis L.)[J]. AIMS Microbiology, 2021, 7(3): 336-353. doi: 10.3934/microbiol.2021021

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Abstract

Endophytes colonizing plant tissue play an essential role in plant growth, development, stress tolerance and plant protection from soil-borne diseases. In this study, we report the diversity of cultivable endophytic bacteria associated with marigold (Calendula officinalis L.) by using 16S rRNA gene analysis and their plant beneficial properties. A total of 42 bacterial isolates were obtained from plant tissues of marigold. They belonged to the genera Pantoea, Enterobacter, Pseudomonas, Achromobacter, Xanthomonas, Rathayibacter, Agrobacterium, Pseudoxanthomonas, and Beijerinckia. Among the bacterial strains, P. kilonensis FRT12, and P. rhizosphaerae FST5 showed moderate or vigorous inhibition against three tested plant pathogenic fungi, F. culmorum, F. solani and R. solani. They also demonstrated the capability to produce hydrolytic enzymes and indole-3-acetic acid (IAA). Five out of 16 isolates significantly stimulated shoot and root growth of marigold in a pot experiment. The present study reveals that more than half of the bacterial isolates associated with marigold (C. officinalis L.) provided antifungal activity against one or more plant pathogenic fungi. Our findings suggest that medicinal plants with antimicrobial activity could be a source for selecting microbes with antagonistic activity against fungal plant pathogens or with plant growth stimulating potential. These isolates might be considered as promising candidates for the improvement of plant health.

Acknowledgments

This research was supported by the CPEA-LT-2016/10095 grant from the Eurasia program of the Norwegian Centre for Cooperation in Education and by the German Academic Exchange Service (DAAD).

Conflict of interest

The authors declare no conflict of interest.

Author contributions:

DE designed the experiment. VS and BA conducted the experiment. SW and VS analysed the data. DE, VS and SDBK wrote the manuscript. KD and SW revised the manuscript and made critical comments. All authors read and approved the manuscript.

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