Icotinib inhibits proliferation and epithelial-mesenchymal transition of non-small cell lung cancer A549 cells

Guohai Wang; Zhiyuan Hu; Guohai Wang; Zhiyuan Hu

doi:10.3934/mbe.2019386

Mathematical Biosciences and Engineering

2019, Volume 16, Issue 6: 7707-7718. doi: 10.3934/mbe.2019386

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Icotinib inhibits proliferation and epithelial-mesenchymal transition of non-small cell lung cancer A549 cells

Guohai Wang ,
Zhiyuan Hu ^,

Department of Cardio-Thoracic Surgery, Zhuji Affiliated Hospital of Wenzhou Medical University, Zhuji 311800, Zhejiang, China

Received: 20 February 2019 Accepted: 10 July 2019 Published: 22 August 2019

ObjectiveTo investigate the inhibitory effect of Icotinib on proliferation and epithelial-mesenchymal transition of A549 cells.

MethodsA549 cells were treated with Icotinib of different concentrations. The morphology of the cells was observed by inverted phase contrast microscope. The effect of different concentrations of Icotinib on the proliferation inhibition rate of A549 cells was detected by MTT assay. Flow cytometry was used to detect effect of Icotinib on the apoptosis rate of A549 cells. ELISA assay was used to detect the expression of EMT-related proteins, E-cadherin, N-cadherin, Vimentin and fibronectin, in A549 cells cultured supernatant. Transwell assay was used to examine the effects of different concentrations of Icotinib on the migration and invasion abilities of A549 cells.

ResultsMorphological changes were observed after A549 cells exposed to different concentrations of Icotinib for 48 hours. The inhibition rate of proliferation for each treatment group increased from MTT assay results. Flow cytometry results showed that cell apoptosis rate was significantly increased. Expression of E-cadherin was up-regulated and expression of N-cadherin, Vimentin and fibronectin were down-regulated from ELISA results. A549 cell migration and invasion abilities were significantly suppressed by Icotinib at different concentrations.

ConclusionIcotinib inhibits A549 cell proliferation, migration and invasion in both concentration and time-dependent manners, in turn it promotes A549 cell apoptosis in the same way. Icotinib also inhibits epithelial-mesenchymal transition by regulating EMT-related proteins expression in A549 cells.
- Icotinib,
- non-small cell lung cancer,
- A549 cells,
- proliferation,
- epithelial-mesenchymal transition
Citation: Guohai Wang, Zhiyuan Hu. Icotinib inhibits proliferation and epithelial-mesenchymal transition of non-small cell lung cancer A549 cells[J]. Mathematical Biosciences and Engineering, 2019, 16(6): 7707-7718. doi: 10.3934/mbe.2019386

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Abstract

ObjectiveTo investigate the inhibitory effect of Icotinib on proliferation and epithelial-mesenchymal transition of A549 cells.

MethodsA549 cells were treated with Icotinib of different concentrations. The morphology of the cells was observed by inverted phase contrast microscope. The effect of different concentrations of Icotinib on the proliferation inhibition rate of A549 cells was detected by MTT assay. Flow cytometry was used to detect effect of Icotinib on the apoptosis rate of A549 cells. ELISA assay was used to detect the expression of EMT-related proteins, E-cadherin, N-cadherin, Vimentin and fibronectin, in A549 cells cultured supernatant. Transwell assay was used to examine the effects of different concentrations of Icotinib on the migration and invasion abilities of A549 cells.

ResultsMorphological changes were observed after A549 cells exposed to different concentrations of Icotinib for 48 hours. The inhibition rate of proliferation for each treatment group increased from MTT assay results. Flow cytometry results showed that cell apoptosis rate was significantly increased. Expression of E-cadherin was up-regulated and expression of N-cadherin, Vimentin and fibronectin were down-regulated from ELISA results. A549 cell migration and invasion abilities were significantly suppressed by Icotinib at different concentrations.

ConclusionIcotinib inhibits A549 cell proliferation, migration and invasion in both concentration and time-dependent manners, in turn it promotes A549 cell apoptosis in the same way. Icotinib also inhibits epithelial-mesenchymal transition by regulating EMT-related proteins expression in A549 cells.

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