Silver nanoclusters stabilized with PVP-BSA conjugate: Optical properties approach

Nataly Arrieta-Sandoval; Juan Francisco Hernández Paz; Imelda Olivas-Armendáriz; Laura Elizabeth Valencia-Gómez; Claudia Alejandra; Rodríguez González; Nataly Arrieta-Sandoval; Juan Francisco Hernández Paz; Imelda Olivas-Armendáriz; Laura Elizabeth Valencia-Gómez; Claudia Alejandra; Rodríguez González

doi:10.3934/matersci.2024010

AIMS Materials Science

2024, Volume 11, Issue 1: 173-199. doi: 10.3934/matersci.2024010

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Silver nanoclusters stabilized with PVP-BSA conjugate: Optical properties approach

Instituto de Ingeniería y Tecnología, Universidad Autónoma de Ciudad Juárez, 450 Del Charro Av., 32310, Ciudad Juárez, Chihuahua, México

Received: 08 September 2023 Revised: 12 December 2023 Accepted: 15 January 2024 Published: 05 February 2024

The objective of this research was to synthesize fluorescent silver nanoclusters (NC Ag-BSA/PVP) using polyvinylpyrrolidone polymer (PVP) as a stabilizer in conjunction with bovine serum albumin protein (BSA). The nanoclusters were prepared using a wet chemistry reduction technique with two distinctive pathways: the addition of PVP after BSA and the addition of PVP after the metal precursor. The optical properties of the materials were studied in samples with different BSA/PVP molar ratios and varying amounts of metal/reductant. The impact of protein and polymer amounts on fluorescence was determined. The materials were characterized using X-ray diffraction (XRD), infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), X-ray energy dispersive spectroscopy (EDS), dynamic light scattering (DLS), fluorescence spectroscopy, and UV-Vis-NIR spectroscopy.

It was observed that increasing the BSA/PVP ratio resulted in higher fluorescence intensity at λ = 450 nm and a decrease at λ = 600 nm. Regarding the metal/reductant ratio, the amount of metal ions impacted the intensity obtained at λ = 600 nm. The experiments revealed that BSA had the largest effect on fluorescence intensity at λ = 450 nm, with little effect on fluorescence intensity regardless of the amount of polymer used. Due to its one-step synthesis and favorable reaction conditions, the NC Ag-BSA/PVP obtained under the proposed methodology holds promise as an optical marker material. The use of the stabilizing duo BSA-PVP, as well as the proposed amounts in this research, serves as a precedent for developing new experimental syntheses of colloidal nanoparticles.
- silver nanoclusters,
- bovine serum albumin,
- polyvinylpyrrolidone,
- full factorial design,
- fluorescence,
- optical properties
Citation: Nataly Arrieta-Sandoval, Juan Francisco Hernández Paz, Imelda Olivas-Armendáriz, Laura Elizabeth Valencia-Gómez, Claudia Alejandra, Rodríguez González. Silver nanoclusters stabilized with PVP-BSA conjugate: Optical properties approach[J]. AIMS Materials Science, 2024, 11(1): 173-199. doi: 10.3934/matersci.2024010

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Abstract

The objective of this research was to synthesize fluorescent silver nanoclusters (NC Ag-BSA/PVP) using polyvinylpyrrolidone polymer (PVP) as a stabilizer in conjunction with bovine serum albumin protein (BSA). The nanoclusters were prepared using a wet chemistry reduction technique with two distinctive pathways: the addition of PVP after BSA and the addition of PVP after the metal precursor. The optical properties of the materials were studied in samples with different BSA/PVP molar ratios and varying amounts of metal/reductant. The impact of protein and polymer amounts on fluorescence was determined. The materials were characterized using X-ray diffraction (XRD), infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), X-ray energy dispersive spectroscopy (EDS), dynamic light scattering (DLS), fluorescence spectroscopy, and UV-Vis-NIR spectroscopy.

It was observed that increasing the BSA/PVP ratio resulted in higher fluorescence intensity at λ = 450 nm and a decrease at λ = 600 nm. Regarding the metal/reductant ratio, the amount of metal ions impacted the intensity obtained at λ = 600 nm. The experiments revealed that BSA had the largest effect on fluorescence intensity at λ = 450 nm, with little effect on fluorescence intensity regardless of the amount of polymer used. Due to its one-step synthesis and favorable reaction conditions, the NC Ag-BSA/PVP obtained under the proposed methodology holds promise as an optical marker material. The use of the stabilizing duo BSA-PVP, as well as the proposed amounts in this research, serves as a precedent for developing new experimental syntheses of colloidal nanoparticles.

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