A review of molecular biology detection methods for human adenovirus

Neelabh Datta; Neelabh Datta

doi:10.3934/biophy.2023008

AIMS Biophysics

2023, Volume 10, Issue 1: 95-120. doi: 10.3934/biophy.2023008

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Review Special Issues

A review of molecular biology detection methods for human adenovirus

Neelabh Datta ^,

Department of Biochemistry, Asutosh College (Affiliated to University of Calcutta), 92, Shyamaprasad Mukherjee Road, Kolkata 700026, West Bengal, India

Received: 03 December 2022 Revised: 05 February 2023 Accepted: 15 February 2023 Published: 10 March 2023

In humans particularly in children, adenovirus is one of the most common viruses that cause respiratory illnesses. Knowing how to detect adenovirus proficiently and rapidly will help reinforce surveillance of adenovirus infections, detect epidemic situations in real-time, and understand the trend of virus epidemics, which will allow effective actions to be taken quickly. The rapid detection of antiviral antibodies or viral antigens in clinical samples can be achieved by molecular diagnostic techniques like PCR, Real-Time PCR, LAMP, mPCR-RLB, PCR-ELISA, Tem-PCR, Gene Chip, and so on. Some of the molecular diagnostic methods are relatively economical, exceedingly sensitive and explicit. There are several commercially accessible molecular diagnostic techniques that enable their use in clinical laboratories all over the world. In this review, the principles, characteristics, and applications of molecular biology surveillance methods commonly used in labs and clinics for the detection of human adenoviruses are examined and highlighted.
- adenovirus,
- PCR,
- real-time PCR,
- LAMP,
- mPCR-RLB,
- PCR-ELISA,
- Tem-PCR,
- gene chip,
- NGS technology,
- MLPA
Citation: Neelabh Datta. A review of molecular biology detection methods for human adenovirus[J]. AIMS Biophysics, 2023, 10(1): 95-120. doi: 10.3934/biophy.2023008

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Abstract

In humans particularly in children, adenovirus is one of the most common viruses that cause respiratory illnesses. Knowing how to detect adenovirus proficiently and rapidly will help reinforce surveillance of adenovirus infections, detect epidemic situations in real-time, and understand the trend of virus epidemics, which will allow effective actions to be taken quickly. The rapid detection of antiviral antibodies or viral antigens in clinical samples can be achieved by molecular diagnostic techniques like PCR, Real-Time PCR, LAMP, mPCR-RLB, PCR-ELISA, Tem-PCR, Gene Chip, and so on. Some of the molecular diagnostic methods are relatively economical, exceedingly sensitive and explicit. There are several commercially accessible molecular diagnostic techniques that enable their use in clinical laboratories all over the world. In this review, the principles, characteristics, and applications of molecular biology surveillance methods commonly used in labs and clinics for the detection of human adenoviruses are examined and highlighted.

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