Effectiveness of synthetic calcite doped with Fe-EDDHSA as a slow-release Fe source: In-vitro experiment on kiwifruit (Actinidia chinensis var. deliciosa) plants

Baldi Elena; Marino Grazia; Muzzi Enrico; Marzadori Claudio; Ciavatta Claudio; Tavoni Marta; Di Giosia Matteo; Calvaresi Matteo; Falini Giuseppe; Zerbetto Francesco; Toselli Moreno; Baldi Elena; Marino Grazia; Muzzi Enrico; Marzadori Claudio; Ciavatta Claudio; Tavoni Marta; Di Giosia Matteo; Calvaresi Matteo; Falini Giuseppe; Zerbetto Francesco; Toselli Moreno

doi:10.3934/agrfood.2019.1.127

AIMS Agriculture and Food

2019, Volume 4, Issue 1: 127-135. doi: 10.3934/agrfood.2019.1.127

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Research article

Effectiveness of synthetic calcite doped with Fe-EDDHSA as a slow-release Fe source: In-vitro experiment on kiwifruit (Actinidia chinensis var. deliciosa) plants

1.
Dipartimento di Scienze e Tecnologie Agro-alimentari, Università di Bologna, Viale Fanin 44, 40127, Bologna, Italy
2.
Dipartimento di Chimica “Giacomo Ciamician”, Università di Bologna, Via F. Selmi 2, Bologna, Italy

Received: 25 October 2018 Accepted: 30 January 2019 Published: 15 February 2019

Doped calcite (Fe-EDDHSA/CaCO₃) was experimentally produced. The hypothesis of the present experiment is that, when roots get in contact with Fe-EDDHSA/CaCO₃, the extrusion of H⁺ decreases the pH and dissolves calcite with subsequent release of Fe that becomes available for roots. The aim of the experiment was to determine whether doped calcite might represent a slow-release Fe source for in-vitro grown kiwifruit plantlets.
The root elongation media used in the experiment had pH 8.0 and differed from each other for Fe supply as follow: Control medium that contained complete Murashige and Skoog salt mixture, including FeSO₄ and Na₂EDTA; calcite medium enriched with Fe-EDDHSA/CaCO₃ as the only Fe source; −Fe medium without Fe.
The absence of FeSO₄ in the medium caused a reduction of plantlet growth. The final pH was higher with calcite medium than in control and −Fe. The addition of Fe-EDDHSA/CaCO₃ increased Fe shoot concentration when compared with the −Fe medium. The data of the present experiment show the potential Fe slow release ability of Fe-EDDHSA/CaCO₃; however, further investigation on Fe containing fertilizers should be conducted on potted plants to validate our results.
- Fe-chelates,
- in-vitro culture,
- pH,
- alkaline growing media,
- EDDHSA/CaCO₃
Citation: Baldi Elena, Marino Grazia, Muzzi Enrico, Marzadori Claudio, Ciavatta Claudio, Tavoni Marta, Di Giosia Matteo, Calvaresi Matteo, Falini Giuseppe, Zerbetto Francesco, Toselli Moreno. Effectiveness of synthetic calcite doped with Fe-EDDHSA as a slow-release Fe source: In-vitro experiment on kiwifruit (Actinidia chinensis var. deliciosa) plants[J]. AIMS Agriculture and Food, 2019, 4(1): 127-135. doi: 10.3934/agrfood.2019.1.127

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Abstract

Doped calcite (Fe-EDDHSA/CaCO₃) was experimentally produced. The hypothesis of the present experiment is that, when roots get in contact with Fe-EDDHSA/CaCO₃, the extrusion of H⁺ decreases the pH and dissolves calcite with subsequent release of Fe that becomes available for roots. The aim of the experiment was to determine whether doped calcite might represent a slow-release Fe source for in-vitro grown kiwifruit plantlets.
The root elongation media used in the experiment had pH 8.0 and differed from each other for Fe supply as follow: Control medium that contained complete Murashige and Skoog salt mixture, including FeSO₄ and Na₂EDTA; calcite medium enriched with Fe-EDDHSA/CaCO₃ as the only Fe source; −Fe medium without Fe.
The absence of FeSO₄ in the medium caused a reduction of plantlet growth. The final pH was higher with calcite medium than in control and −Fe. The addition of Fe-EDDHSA/CaCO₃ increased Fe shoot concentration when compared with the −Fe medium. The data of the present experiment show the potential Fe slow release ability of Fe-EDDHSA/CaCO₃; however, further investigation on Fe containing fertilizers should be conducted on potted plants to validate our results.

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