Lack of Association between Human µ-Opioid Receptor (<em>OPRM1</em>) Gene Polymorphisms and Heroin Addiction in A Sample of Southeast Iranian Population

Mansour Shakiba; Mohammad Hashemi; Zahra Rahbari; Salah Mahdar; Hiva Danesh; Fatemeh Bizhani; Gholamreza Bahari; Mansour Shakiba; Mohammad Hashemi; Zahra Rahbari; Salah Mahdar; Hiva Danesh; Fatemeh Bizhani; Gholamreza Bahari

doi:10.3934/medsci.2017.2.233

AIMS Medical Science

2017, Volume 4, Issue 2: 233-240. doi: 10.3934/medsci.2017.2.233

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Research article

Lack of Association between Human µ-Opioid Receptor (OPRM1) Gene Polymorphisms and Heroin Addiction in A Sample of Southeast Iranian Population

1.
Health Promotion Research Center, Zahedan University of Medical Sciences, Zahedan, Iran;
2.
Department of Psychiatry, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran;
3.
Cellular and Molecular Research Center, Zahedan University of Medical Sciences, Zahedan, Iran;
4.
Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran

Received: 17 March 2017 Accepted: 14 May 2017 Published: 24 June 2017

It has been proposed that genetic factors account for 30%–50% of the risk for cocaine and heroin addiction. The present study was aimed to find out the impact of µ-opioid receptor gene (OPRM1) rs1799971 A > G and rs9479757 polymorphisms on heroin dependence in a sample of southeast Iranian population. This case-control study was done on 123 heroin addicts and 140 non-addicts Iranian male. Genomic DNA was extracted from peripheral blood cells using salting out method. Genotyping of OPRM1 rs1799971 and rs9479757 polymorphisms were performed using PCR-RFLP method. Overall, our results did not support an association between OPRM1 variants and risk of heroin dependence in a sample of southeast Iranian population. Further studies with larges sample sizes and different ethnicities are required to validate our findings.
- OPRM1,
- polymorphism,
- addiction,
- heroin
Citation: Mansour Shakiba, Mohammad Hashemi, Zahra Rahbari, Salah Mahdar, Hiva Danesh, Fatemeh Bizhani, Gholamreza Bahari. Lack of Association between Human µ-Opioid Receptor (OPRM1) Gene Polymorphisms and Heroin Addiction in A Sample of Southeast Iranian Population[J]. AIMS Medical Science, 2017, 4(2): 233-240. doi: 10.3934/medsci.2017.2.233

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Abstract

It has been proposed that genetic factors account for 30%–50% of the risk for cocaine and heroin addiction. The present study was aimed to find out the impact of µ-opioid receptor gene (OPRM1) rs1799971 A > G and rs9479757 polymorphisms on heroin dependence in a sample of southeast Iranian population. This case-control study was done on 123 heroin addicts and 140 non-addicts Iranian male. Genomic DNA was extracted from peripheral blood cells using salting out method. Genotyping of OPRM1 rs1799971 and rs9479757 polymorphisms were performed using PCR-RFLP method. Overall, our results did not support an association between OPRM1 variants and risk of heroin dependence in a sample of southeast Iranian population. Further studies with larges sample sizes and different ethnicities are required to validate our findings.

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