Minimizing <em>Pseudomonas aeruginosa</em> adhesion to titanium surfaces by a plasma nitriding process

Michelle de Medeiros Aires; Janine Treter; Antônio Nunes Filho; Igor Oliveira Nascimento; Alexandre José Macedo; Clodomiro Alves Júnior; Michelle de Medeiros Aires; Janine Treter; Antônio Nunes Filho; Igor Oliveira Nascimento; Alexandre José Macedo; Clodomiro Alves Júnior

doi:10.3934/biophy.2017.1.19

AIMS Biophysics

2017, Volume 4, Issue 1: 19-32. doi: 10.3934/biophy.2017.1.19

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Research article

Minimizing Pseudomonas aeruginosa adhesion to titanium surfaces by a plasma nitriding process

1.
Universidade Federal do Rio Grande do Norte, LabPlasma, 59072-970, Rio Grande do Norte, Brazil
2.
Universidade Federal do Rio Grande do Sul, Centro de Biotecnologia and Faculdade de Farmácia, Laboratório de Biofilmes e Diversidade Microbiana, 91501-970, Rio Grande do Sul, Brazil

Received: 04 October 2016 Accepted: 12 December 2016 Published: 23 December 2016

The research of the interaction between bacteria-surface has great importance for titanium biomedical applications once microorganisms offer risks because promoting implant loss. Therefore, study bacterial adhesion and colonization on titanium is interesting because are principal factors infections pathogeny on biomaterials. In this study, commercial grade II titanium was submitted to nitriding treatment to plasma at 2.2 mbar, using gas mixtures of 80% hydrogen (H2) and 20% nitrogen (N2) during 1 hour and 3 hour. The surfaces were physically and chemically characterized. In order to evaluate bacterial response, the surfaces were exposed to Pseudomonas aeruginosa. The titanium surface modified in nitriding plasma, although exposes a higher roughness as compared with untreated samples, exhibited lower bacterial growth. The nitrided sample for 3 hour exhibited the higher amount of TiN phase and the higher concentration of atomic nitrogen on surface and lower bacterial adhered count. These results were confirmed by scanning electron microscopy. Based on these results can be said to the thermochemical treatment of plasma nitriding on titanium samples results a significant reduction of adherence of Pseudomonas aeruginosa. It was found that the Ti surface nitrided offers significant reduction of bacterial adherence which prevent biofilm formation and offersing lower risk of infection and implant remotion.
- Pseudomonas aeruginosa,
- biofilm,
- plasma,
- titanium,
- thermochemical treatment
Citation: Michelle de Medeiros Aires, Janine Treter, Antônio Nunes Filho, Igor Oliveira Nascimento, Alexandre José Macedo, Clodomiro Alves Júnior. Minimizing Pseudomonas aeruginosa adhesion to titanium surfaces by a plasma nitriding process[J]. AIMS Biophysics, 2017, 4(1): 19-32. doi: 10.3934/biophy.2017.1.19

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Abstract

The research of the interaction between bacteria-surface has great importance for titanium biomedical applications once microorganisms offer risks because promoting implant loss. Therefore, study bacterial adhesion and colonization on titanium is interesting because are principal factors infections pathogeny on biomaterials. In this study, commercial grade II titanium was submitted to nitriding treatment to plasma at 2.2 mbar, using gas mixtures of 80% hydrogen (H2) and 20% nitrogen (N2) during 1 hour and 3 hour. The surfaces were physically and chemically characterized. In order to evaluate bacterial response, the surfaces were exposed to Pseudomonas aeruginosa. The titanium surface modified in nitriding plasma, although exposes a higher roughness as compared with untreated samples, exhibited lower bacterial growth. The nitrided sample for 3 hour exhibited the higher amount of TiN phase and the higher concentration of atomic nitrogen on surface and lower bacterial adhered count. These results were confirmed by scanning electron microscopy. Based on these results can be said to the thermochemical treatment of plasma nitriding on titanium samples results a significant reduction of adherence of Pseudomonas aeruginosa. It was found that the Ti surface nitrided offers significant reduction of bacterial adherence which prevent biofilm formation and offersing lower risk of infection and implant remotion.

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