Characterization of <em>Lactobacillus, Bacillus</em> and <em>Saccharomyces</em> isolated from Iranian traditional dairy products for potential sources of starter cultures

Farzad Rahmati; Farzad Rahmati

doi:10.3934/microbiol.2017.4.815

AIMS Microbiology

2017, Volume 3, Issue 4: 815-825. doi: 10.3934/microbiol.2017.4.815

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Research article

Characterization of Lactobacillus, Bacillus and Saccharomyces isolated from Iranian traditional dairy products for potential sources of starter cultures

Farzad Rahmati ^,

Department of Microbiology, Faculty of Science, Qom Branch, Islamic Azad University, Qom, IR Iran

Received: 06 August 2017 Accepted: 29 September 2017 Published: 12 October 2017

The aim of the present study was to investigate technological properties of starter strains from traditional dairy products collected from five villages of Lorestan province in Iran. Thirty five samples were cultured on selective media (MRS broth, Nutrient Broth and YGC and then typical colonies checked for morphological features and eventually eighty two strains selected for further examination. The strains were evaluated for Hydrolysis of casein, starch and citrate, growth at 15 and 45 °C, growth in 4 and 6.5% NaCl, resistance to antibiotics (ampicillin, bacitracin, chloramphenicol, erythromycin, gentamicin, penicillin, novobiocin, nalidixic acid) proteolytic and lipolytic and acidification activities. Sixteen strains chosen according to the difference in cell morphology and were identified using API galleries and ability to metabolize various carbohydrates, which consequently, led to identifying seven Lactobacillus casei, five Lactobacillus plantarum, two Saccharomyces cerevisiae and two Bacillus subtilis. In general, two strains of L. casei AKL2, DDL2, two strains of L. plantarum SYL5, ACL4 and one strain of S. cerevisiae DDy2 was demonstrated the most important technological characterization that suitable for using as starter cultures.
- Bacillus,
- Lactobacillus,
- Saccharomyces,
- starter cultures,
- technological properties
Citation: Farzad Rahmati. Characterization of Lactobacillus, Bacillus and Saccharomyces isolated from Iranian traditional dairy products for potential sources of starter cultures[J]. AIMS Microbiology, 2017, 3(4): 815-825. doi: 10.3934/microbiol.2017.4.815

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Abstract

The aim of the present study was to investigate technological properties of starter strains from traditional dairy products collected from five villages of Lorestan province in Iran. Thirty five samples were cultured on selective media (MRS broth, Nutrient Broth and YGC and then typical colonies checked for morphological features and eventually eighty two strains selected for further examination. The strains were evaluated for Hydrolysis of casein, starch and citrate, growth at 15 and 45 °C, growth in 4 and 6.5% NaCl, resistance to antibiotics (ampicillin, bacitracin, chloramphenicol, erythromycin, gentamicin, penicillin, novobiocin, nalidixic acid) proteolytic and lipolytic and acidification activities. Sixteen strains chosen according to the difference in cell morphology and were identified using API galleries and ability to metabolize various carbohydrates, which consequently, led to identifying seven Lactobacillus casei, five Lactobacillus plantarum, two Saccharomyces cerevisiae and two Bacillus subtilis. In general, two strains of L. casei AKL2, DDL2, two strains of L. plantarum SYL5, ACL4 and one strain of S. cerevisiae DDy2 was demonstrated the most important technological characterization that suitable for using as starter cultures.

References

[1]	Rodrı́guez E, González B, Gaya P, et al. (2000) Diversity of bacteriocins produced by lactic acid bacteria isolated from raw milk. Int Dairy J 10: 7–15. doi: 10.1016/S0958-6946(00)00017-0
[2]	Temmerman R, Huys G, Swings J (2004) Identification of lactic acid bacteria: culture-dependent and culture-independent methods. Trends Food Sci Tech 15: 348–359. doi: 10.1016/j.tifs.2003.12.007
[3]	Ghobadi DM, Yakhchali AH, Salmanian B, et al. (2011) High-level acetaldehyde production by an indigenous Lactobacillus strain obtained from traditional dairy products of Iran. Afr J Microbiol Res 5: 4398–4405.
[4]	Litopoulou-Tzanetaki E, Tzanetakis N (1992) Microbiological study of white-brined cheese made from raw goat milk. Food Microbiol 9: 13–19. doi: 10.1016/0740-0020(92)80057-B
[5]	Abdi R, Sheikh-Zeinoddin M, Soleimanian-Zad S (2006) Identification of lactic acid bacteria isolated from traditional Iranian Lighvan cheese. Pak J Biol Sci 9: 99–103. doi: 10.3923/pjbs.2006.99.103
[6]	Erkkila S, Petaja E (2000) Screening of commercial meat starter cultures at low pH and in the presence of bile salts for potential probiotic use. Meat Sci 55: 297–300. doi: 10.1016/S0309-1740(99)00156-4
[7]	Anderson NL, Noble MA, Weissfeld AS, et al. (2005) In: Sewell DL, Editor, Quality systems in the clinical microbiology laboratory, Washington, D.C: American Society for Microbiology, 80–82.
[8]	Harrigan W, McCance M (1976) Biochemical test for bacteria, In: Laboratory methods in food and dairy microbiology, 2 Eds., London: Academic Press Inc, 15–22.
[9]	Swearingen P, O'sullivan D, Warthesen J (2001) Isolation, characterization, and influence of native, nonstarter lactic acid bacteria on Cheddar cheese quality. J Dairy Sci 84: 50–59. doi: 10.3168/jds.S0022-0302(01)74451-7
[10]	Ray P, Chatter JK, Chakraborty C, et al. (2013) Lipolysis of milk: A review. IJASVM 1: 58–74.
[11]	Smibert R, Krieg N (1981) General characterization, In: Manual of methods for general bacteriology, 409–443.
[12]	Akabanda F, Owusu-Kwarteng J, Tano-Debrah K, et al. (2014) The use of lactic acid bacteria starter culture in the production of Nunu, a spontaneously fermented milk product in Ghana. Int J Food Sci 2014: 217.
[13]	Balows A, Truper HG, Dworkin M, et al. (1991) The Procaryotes, 2Eds., New York: Springer-Verlag, 385–389.
[14]	Fguiri I, Ziadi M, Atigui M, et al. (2016) Isolation and characterization of lactic acid bacteria strains from raw camel milk for potential use in the production of fermented Tunisian dairy products. Int J Dairy Technol 69: 103–113. doi: 10.1111/1471-0307.12226
[15]	Amor KB, Vaughan EE, de Vos WM (2007) Advanced molecular tools for the identification of lactic acid bacteria. J Nutr 137: 741S.
[16]	Sharma P, Tomar SK, Sangwan V, et al. (2016) Antibiotic resistance of Lactobacillus sp. isolated from commercial probiotic preparations. J Food Safety 36: 38–51.
[17]	Ayad E, Nashat S, El-Sadek N, et al. (2004) Selection of wild lactic acid bacteria isolated from traditional Egyptian dairy products according to production and technological criteria. Food Microbiol 21: 715–725.
[18]	Vuillemard J, Amiot J, Gauthier S (1986) Evaluation de l'activité protéolytique de bactéries lactiques par une méthode de diffusion sur plaque. Microbiol Alim Nutr 3: 327–332.
[19]	Ma C, Zhang L, Ma D, et al. (2012) Technological characterization of Lactobacilli isolated from Chinese artisanal fermented milks. Int J Dairy Technol 65: 132–139. doi: 10.1111/j.1471-0307.2011.00743.x
[20]	Khalid NM, Marth EH (1990) Lactobacilli-their enzymes and role in ripening and spoilage of cheese: a review. J Dairy Sci 73: 2669–2684. doi: 10.3168/jds.S0022-0302(90)78952-7
[21]	Oterholm A, Ordal ZJ, Witter LD (1968) Glycerol ester hydrolase activity of lactic acid bacteria. Appl Microbiol 16: 524–527.
[22]	Kayagil F (1976) Effect of traditional starter cultures on quality of cheese (Ms.c Thesis), Ankara: Middle east technical university.
[23]	Mangia NP, Murgia MA, Garau G, et al. (2008) Influence of selected lab cultures on the evolution of free amino acids, free fatty acids and Fiore Sardo cheese microflora during the ripening. Food Microbiol 25: 366–377.
[24]	Vélez MP, Hermans K, Verhoeven T, et al. (2007) Identification and characterization of starter lactic acid bacteria and probiotics from Columbian dairy products. J Appl Microbiol 103: 666–674. doi: 10.1111/j.1365-2672.2007.03294.x
[25]	Aplevicz KS, Mazo JZ, Ilha EC, et al. (2014) Isolation and characterization of lactic acid bacteria and yeasts from the Brazilian grape sourdough. Braz J Pharm Sci 50: 321–327. doi: 10.1590/S1984-82502014000200011

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