Angiotensin II induces the secretion of ICAM-1 and MCP-1 in human airway smooth muscle cells in vitro

Ning Li; Yating Huo; Haojun Xie; Yuanxiong Cheng; Ning Li; Yating Huo; Haojun Xie; Yuanxiong Cheng

doi:10.3934/medsci.2018.3.259

AIMS Medical Science

2018, Volume 5, Issue 3: 259-267. doi: 10.3934/medsci.2018.3.259

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Research article

Angiotensin II induces the secretion of ICAM-1 and MCP-1 in human airway smooth muscle cells in vitro

1.
Department of Neurosurgery, The First Affiliated Hospital, Jinan University, Guangzhou, Guangdong, China
2.
Department of Respiratory Disease, The Third Affiliated Hospital, Southern Medical University, Guangzhou, China

Received: 23 March 2018 Accepted: 11 June 2018 Published: 27 June 2018

Background: Angiotensin [1] II is known to cause human airway smooth muscle (HASM) contraction and closely relate to asthma, however, the effect of Ang II on HASM cells (HASMCs) secretion function is still unclear. Methods: Primary cultured HASMCs were treated with Ang II, Ang II + Ang-(1–7), and Ang II + irbersatan (IRB), respectively. The nuclear translocation of the NF-κB p65 in HASMCs in each group was analyzed performed with Immunofluorescence. The expression levels of intercellular adhesion molecule-1 (ICAM-1) and monocyte chemoattractant protein-1 (MCP-1) were evaluated by Real-time polymerase chain reaction (PCR) and Enzyme linked immunosorbent assay (ELISA). Results: Ang II caused sharply increase of the nuclear translocation of NF-κB p65, also, Ang II significantly increased expression of ICAM-1 and MCP-1 secreted by HASMCs, this effect could be inhibited by Ang-(1–7) and IRB. Conclusion: These data may indicate the effect of Ang II on inducing HASMCs to secret ICAM-1 and MCP-1, which might be through the NF-κB p65 pathway by AT-1 receptor.
- human airway smooth muscle cells,
- angiotensin II,
- ICAM-1,
- MCP-1,
- NF-κB signal pathway
Citation: Ning Li, Yating Huo, Haojun Xie, Yuanxiong Cheng. Angiotensin II induces the secretion of ICAM-1 and MCP-1 in human airway smooth muscle cells in vitro[J]. AIMS Medical Science, 2018, 5(3): 259-267. doi: 10.3934/medsci.2018.3.259

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Abstract

Background: Angiotensin [1] II is known to cause human airway smooth muscle (HASM) contraction and closely relate to asthma, however, the effect of Ang II on HASM cells (HASMCs) secretion function is still unclear. Methods: Primary cultured HASMCs were treated with Ang II, Ang II + Ang-(1–7), and Ang II + irbersatan (IRB), respectively. The nuclear translocation of the NF-κB p65 in HASMCs in each group was analyzed performed with Immunofluorescence. The expression levels of intercellular adhesion molecule-1 (ICAM-1) and monocyte chemoattractant protein-1 (MCP-1) were evaluated by Real-time polymerase chain reaction (PCR) and Enzyme linked immunosorbent assay (ELISA). Results: Ang II caused sharply increase of the nuclear translocation of NF-κB p65, also, Ang II significantly increased expression of ICAM-1 and MCP-1 secreted by HASMCs, this effect could be inhibited by Ang-(1–7) and IRB. Conclusion: These data may indicate the effect of Ang II on inducing HASMCs to secret ICAM-1 and MCP-1, which might be through the NF-κB p65 pathway by AT-1 receptor.

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