Surface plasmon resonance imaging of the conversion of clustered DNA lesions into double strand breaks by Fpg protein

Muriel Jourdan; Julia Pingel; Arnaud Buhot; Thierry Livache; Jean-François Constant; Muriel Jourdan; Julia Pingel; Arnaud Buhot; Thierry Livache; Jean-François Constant

doi:10.3934/matersci.2015.4.473

AIMS Materials Science

2015, Volume 2, Issue 4: 473-483. doi: 10.3934/matersci.2015.4.473

Previous Article Next Article

Research article Topical Sections

Surface plasmon resonance imaging of the conversion of clustered DNA lesions into double strand breaks by Fpg protein

1.
Univ. Grenoble Alpes, UMR 5250, F-38000 Grenoble, France CNRS, DCM UMR 5250, F-38000 Grenoble, France;
2.
Univ. Grenoble Alpes, INAC, SPrAM, F-38000 Grenoble, France CEA, INAC, SPrAM, F-38000 Grenoble, France CNRS, SPrAM, F-38000 Grenoble, France;
3.
Current address: DKMS German Bone Marrow Donor Center, 72072 Tübingen, Germany

Received: 01 October 2015 Accepted: 12 November 2015 Published: 16 November 2015

Ionizing radiations induce clustered damage sites known to be severely challenging for the cell's repair machinery. In the present study, we have grafted on a biochip four synthetic oligonucleotide duplexes containing either a single 8-oxoguanine, a single 2'-deoxyribose abasic site or two different clustered damages containing these two lesions on opposite strands. Using a SPR imaging-based DNA array, we qualitatively observed the hierarchy of lesion processing by the base excision repair (BER) enzyme Fpg (Formamidopyrimidine (fapy)-DNA glycosylase). We could notably show that Fpg can convert a cluster of lesions into a potentially lethal double strand break (DSB) and demonstrate that SPR imaging is suitable to investigate the incision steps occurring during the repair process.
- surface plasmon resonance imaging,
- DNA double strand break,
- Fpg,
- 8-oxoguanine,
- abasic site,
- DNA cluster lesion
Citation: Muriel Jourdan, Julia Pingel, Arnaud Buhot, Thierry Livache, Jean-François Constant. Surface plasmon resonance imaging of the conversion of clustered DNA lesions into double strand breaks by Fpg protein[J]. AIMS Materials Science, 2015, 2(4): 473-483. doi: 10.3934/matersci.2015.4.473

Related Papers:

Abstract

Ionizing radiations induce clustered damage sites known to be severely challenging for the cell's repair machinery. In the present study, we have grafted on a biochip four synthetic oligonucleotide duplexes containing either a single 8-oxoguanine, a single 2'-deoxyribose abasic site or two different clustered damages containing these two lesions on opposite strands. Using a SPR imaging-based DNA array, we qualitatively observed the hierarchy of lesion processing by the base excision repair (BER) enzyme Fpg (Formamidopyrimidine (fapy)-DNA glycosylase). We could notably show that Fpg can convert a cluster of lesions into a potentially lethal double strand break (DSB) and demonstrate that SPR imaging is suitable to investigate the incision steps occurring during the repair process.

References

[1]	Magnander K,Elmroth K(2012)Biological consequences of formation and repair of complex DNA damage.Cancer Lett 327: 90-96.
[2]	Eccles LJ, O'Neill P, Lomax ME (2011) Delayed repair of radiation induced clustered DNA damage: Friend or foe? MutatRes 711: 134-141.
[3]	Sage E, Harrison L (2011) Clustered DNA lesion repair in eukaryotes: Relevance to mutagenesis and cell survival.MutatRes 711: 123-133.
[4]	Okayasu R (2012) Repair of DNA damage induced by accelerated heavy ions-a mini review.IntJCancer 130: 991-1000.
[5]	Georgakilas AG, O'Neill P, Stewart RD (2013) Induction and repair of clustered DNA lesions: what do we know so far?RadiatRes 180: 100-109.
[6]	Pouget JP, Frelon S,Ravanat JL,et al. (2002) Formation of modified DNA bases in cells exposed either to gamma radiation or to high-LET particles.RadiatRes 157: 589-595.
[7]	Cadet J,Douki T,Ravanat JL(2008) Oxidatively generated damage to the guanine moiety of DNA: mechanic aspects and formation in cells.AccChemRes 41: 1075-1083.
[8]	Frelon S,Douki T,Ravanat JL,et al. (2000) High-performance liquid chromatography-tandem mass spectrometry measurement of radiation-induced base damage to isolated and cellular DNA.ChemResToxicol 13: 1002-1010.
[9]	Memisoglu A, Samson L(2000) Base excision repair in yeast and mammals.MutatRes 451: 39-51.
[10]	Wilson DM, Sofinowski TM and McNeill DR (2003) Repair mechanisms for oxidative DNA damage.FrontBiosci 8:963-981.
[11]	Shikazono N,Noguchi M,Fujii K,et al. (2009) The Yield, processing, and biological consequences of custered DNA camage induced by ionizing radiation.JRadiatRes 50: 27-36.
[12]	Blaisdell JO, Wallace SS (2001) Abortive base-excision repair of radiation-induced clustered DNA lesions in Escherichia coli.ProcNatlAcadSciUSA 98: 7426-7430. doi: 10.1073/pnas.131077798
[13]	D'Souza DI, Harrison L(2003) Repair of clustered uracil DNA damages in Escherichia coli.Nucleic Acids Res 31: 4573-4581.
[14]	Lomax ME,Cunniffe S,O'Neill P(2004) Efficiency of repair of an abasic site within DNA clustered damage sites by mammalian cell nuclear extracts.DNA Repair 43: 11017-11026.
[15]	Pearson CG,Shikazono N,Tacker J,et al. (2004) Enhanced mutagenic potential of 8-oxo-7,8-dihydroguanine when present within a clustered DNA damage site.Nucleic Acids Res 32: 263-270. doi: 10.1093/nar/gkh150
[16]	Budworth H,Dianova II,Podust VN,et al. (2002) Repair of clustered DNA lesions.JBiolChem 277: 21300-21305.
[17]	Tian K,McTigue M, de los Santos C(2002) Sorting the consequences of ionizing radiation: processing of 8-oxoguanine/abasic site lesions.DNA Repair 1: 1039-1049.
[18]	Corne C, Fiche JB,Gasparutto D,et al. (2008) SPR imaging for label-free multiplexed analyses of DNA N-glycosylase interactions with damage DNA duplexes.Analyst 133: 1036-1045. doi: 10.1039/b801086b
[19]	David-Cordonnier MH, Laval J, O'Neill P (2001) Recognition and kinetics for excision of a base lesion within clustered DNA damage by the Escherichia coli proteins Fpg and Nth.Biochemistry 40: 5738-5746. doi: 10.1021/bi002605d
[20]	David-Cordonnier MH, Laval J, O'Neill P(2000) Clustered DNA damage, influence on damage excision by XRS5 nuclear extracts and Escherichia coli Nth and Fpg proteins.JBiolChem 275: 11865-11873.
[21]	Harisson L, Hatahet Z, Wallace SS (1999) In vitro repair of synthetic ionizing radiation-induced multiply damaged DNA sites.JMolBiol 290: 667-684.
[22]	Zálešák J,Lourdin M,Krejčί L,et al. (2014) Structure and dynamics of DNA duplexes containing a cluster of mutagenic 8-oxoguanine and abasic site lesions.JMolBiol 426: 1524-1538.
[23]	BhartiSK,Varshney U (2010) Analysis of the impact of a uracil DNA glycosylaseattenuated in AP-DNA binding in maintenance of the genomic integrity in Escherichia coli.Nucleic Acids Res 38: 2291-2301 doi: 10.1093/nar/gkp1210

Reader Comments

Your name:*

Email:*
© 2015 the Author(s), licensee AIMS Press. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0)